25 October 2014, Volume 40 Issue 10
    

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    Food and Fermentation Industries
  • LIU Wen-ying, GU Rui-zeng, LIN Feng, LU Jun, CAI Mu-yi
    Food and Fermentation Industries. 2014, 40(10): 1-5. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.001
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    Iron-chelating black-bone silky fowl oligopeptides(BSFP-Fe) were prepared by using black-bone silky fowl as raw material.Taking molecular weight distribution and iron content as indicators,the effects of temperature,pH and in vitro simulated gastrointestinal digestion on the stability of BSFP-Fe were investigated.Results showed that the change of the molecular weight distribution ranges did not exceed 2% and the iron content of BSFP-Fe showed no significant difference under the conditions of temperature 20 ~ 80℃.Under the conditions of pH 3 ~ 9,the molecular weights below 1000 u of BSFP-Fe were slightly elevated,but the content did not exceed 6%.The iron content of BSFP-Fe decreased,but was still higher than 58.4%.After digested by proteases,the molecular weights below 1000 u of BSFP-Fe were slightly elevated,but the content did not exceed 8%.After digested by pepsin and trypsin together,the iron content of BSFP-Fe was 37.6%.The results indicated that BSFP-Fe had certain thermal stability,pH stability and digestion stability.
  • XU Guo-qiang, CHEN Xiu-lai, WU Man-zhen
    Food and Fermentation Industries. 2014, 40(10): 6-10. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.016
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    Four-carbon dicarboxylic acids are widely used in food,pharmaceutical and chemical industries.Thus there is a huge market potential for then.S.cerevisiae is regarded as the best candidate microorganism for producing four-carbon dicarboxylic acids.Unfortunately,there is strong tendency for ethanol formation under fully aerobic conditions when sugar is present in access in batch culture,which leads to the loss of carbon flow.To reduce ethanol formation,the positive regulate gene THI2 in thiamine synthetic pathway was deleted,and the ethanol titer decreased from 5.27 ± 0.23 g /L to 0.53 ± 0.12 g /L.However,this strategy led to a week growth and glucose consumption.Then cell growth and glucose consumption were improved but still weak when 0.04 μmol /L ThDP was added.To further improve cell growth and glucose consumption,the effect of NAD+on FMME-002△THI2 was explored.Results show that glucose consumption and cell growth were improved by 48.6% and 47.2%,respectively,while ethanol titer only increased 0.56 g /L when 1 000 μg /L NAD+was added.In brief,ethanol formation was reduced efficiently by regulating the level of cofactors thiamine and NAD+,which provideed a novel strategy to solve this universal problem in the field of production of four-carbon dicarboxylic acids from S.cerevisiae.
  • ZHOU Wan-hai, WEI Qin, WANG Tao, FENG Rui-zhang, DU Yong-hua, PANG Jian-yi
    Food and Fermentation Industries. 2014, 40(10): 11-15. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.047
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    In order to study the effect of inoculating microbes on degradation and maturity of composting,rnitrogen-fixation bacteria(NF),cellulose-decomposing bacteria(CD),nitrogen-fixation bacteria plus cellulose-decomposing bacteria(NF + CD) were inoculated in vinegar dregs.Then,temperature,moisture,pH value,total organic carbon,crude fibre and seed germination index were determined across the composting process.The results showed that the rise of temperature,water loss and pH change of NF + CD appeared earlier and more obviously compared with other treatments,and NF + CD entered mature period 5-6 days ahead than NF and CD,13 days ahead than CK.After the fermentation accomplished,the content of total organic carbon and crude fiber of NF + CD decreased 46.22% and 28.1%respectively.Moreover,for NF and CD,the degradation efficiency of the total organic carbon and crude fiber was markedly higher than that in CK,although less than that in NF + CD,and the degradation efficiency of crude fiber reached 36.8% in treatment of CD.The seed germination index was over 80% after 42 d,49d and 49 d,when inoculated NF + CD,NF and CD respectively,while the CK needed 56 d.Therefore,composting with microbial inoculation could improve temperature of materials at the initial stage,advance the period of high temperature,increase the accumulated temperature,which stimulated the moisture loss,degradation of total organic carbon and crude fiber,improved seed germination index,and shortened the composting period.In addition,the effect of nitrogen-fixation bacteria and cellulosedecomposing bacteria inoculating mixed on vinegar dregs composing were more prominent than they inoculating singly.
  • QI Xiang-hui, WANG Xu, LIN Jing, ZHU Jing-fei, LUO Yan, SUN Wen-jing
    Food and Fermentation Industries. 2014, 40(10): 16-21. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.048
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    By a screening method with high concentration of D-glucose in the medium,99 osmophilic yeasts were isolated from crude honeycomb and honey.Then,40 strains isolated from different samples were fermented in flaskshaking to study the variety of polyol in the fermentation broth by TLC(Thin Layer Chromatography) analysis methods.An effective method was established in this study to separate D-glucose and polyol.The qualitative detection of D-arabitol and byproducts was performed by TLC using a developing solvent system of normal ethyl acetate /pyridine /glacial acetic acid /water(16∶ 10∶ 2∶ 3) on microcrystalline cellulose plate.Experimental results showed that most of these yeasts were D-arabitol-producing and glycerol-producing strains.According to the observation and analyses of morphological,physiological and biochemical characteristics,ITS sequences and the established phylogenetic tree,the three different colonies were identified as Lodderomyces elongisporus,Zygosaccharomyces rouxii and Starmerella sp..A high-performing strain was isolated by screening tests,and the yield of D-arabitol was 61.33 ± 0.49 g /L,which detected by gas chromatography.The above results laid a foundation for further study on D-arabitol production by yeast.
  • LEI Jun-hua, GAO Qun-yu
    Food and Fermentation Industries. 2014, 40(10): 22-26. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.017
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    The native waxy rice starch was applied to prepare dextrin with different hydrolysis degrees by using β-amylase at different time,and the physicochemical properties of the dextrin were also investigated.Results showed that starch treated with β-amylase for 20 hours reached the maximum starch hydrolysis rate.The results from Scanning electron microscopy(SEM) showed that all enzyme-treated samples had more irregular fragments without granular structures.The maximum absorbance and iodine binding of enzyme-treated starches were reduced compared with their native starch parents.The position of maximum absorption peak of starch sample shifted obviously as the increasing degree of hydrolysis.With the prolonging time of hydrolysis,macromoleculars were reduced and small molecules were increased.At the same temperature,the solubilities of enzyme treated starchs were higher than that of native starch,which was different from the swelling power.Meanwhile,the solubility and swelling power increased with the increase of hydrolysis degree and the temperature.Compared with native waxy rice starch,the enzyme-treated samples revealed more remarkable improvement on transparency;but which were constantly descending with the hydrolysis degree increasing.
  • FAN Jin-bo, CAI Xi-tong, HOU Yu, ZHOU Su-zhen, FENG Xu-qiao
    Food and Fermentation Industries. 2014, 40(10): 27-31. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.049
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    α-Lactalbumin(α-LA) is one of the main allergens in cow milk.In this study,α-LA was glycated with different size saccharides(glucose,maltose,maltotriose,maltotetraose and maltopentaose) to study the effect of the size of the saccharide on protein antigenicity.The results showed that,with an increase in the size of the saccharide,the degree of glycation decreased.The antigenicity of all glycated samples significantly decreased with an increase in incubation time,suggesting that the extent of glycation might be an important factor to decrease antigenicity.However,the largest reduction of antigenicity was observed after incubation with maltotriose,whose antigenicity declined by 51.67%.This could be related to the difference in binding specificity of the saccharides to the allergenic sites of the protein.Through comparing the glycation sites and the reduction of the antigenicity by incubating with the five saccharides,we found Lys58 may play an important role in the reduction of the antigenicity of α-LA.The results suggested that the glycation size could affect the glycation site,and eventually impact the antigenicity of the glycated protein.When the main epitope was glycated,the degree of antigenicity reduction increased with the increase in the size of the saccharide.
  • ZHAO Lei, LI Si-ran, ZHANG He-yan, HAO Tian-yang, WU Di, WANG Hong
    Food and Fermentation Industries. 2014, 40(10): 32-36. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.050
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    The objective of this study was to investigate the antifungal activities of sugar fatty acid esters against food spoilage fungi strains.Sugar fatty acid esters were synthesized by immobilized lipase in acetone in the presence of molecular sieve and further purified by slica-gel column chromatography.The susceptibility of four strains of fungi(Penicilium oxalicum,Aspergillus tubingensis,Mucor circinelloides,Rhizopus nigricans) to different sugar fatty acid esters at different pH values were evaluated.Fructose monolaurate had the highest antifungal activity against Penicilium oxalicum and Aspergillus tubingensis.The antifungal activity enhanced with increasing concentration of fructose monolaurate.Fructose monolaurate affected not only the size,but also the thickness and pigment production of mold colony.The pH value had a significant impact on antifungal activity.The antifungal activity of fructose monolaurate under neutral conditions was lower than that under acidic and alkaline conditions.
  • MA Teng-zhen, YANG Xue-shan, ZHANG Li, ZHANG Yan-fang, LI Ying, ZHU Xia, WANG Jing, HAN Shun-yu
    Food and Fermentation Industries. 2014, 40(10): 37-42. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.051
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    p-NPG(4-nitrophenyl-β-D-glucopyranoside) method is commonly used for β-glucosidase activity determination,however,quantifying conditions significantly influence enzyme activity,not only decrease the reliability and authenticity of results,but also disadvantaged to the comparability among different papers.In order to build β-glucosidase activity determination conditions which suit for wine yeast,a selected yeast Vintage Red was used to test the influence of reaction time,temperature,substrate concentration and pH values on the activity of glucosidase by single factor and orthogonal tests.The results showed that the best conditions for quantifying β-glucosidase activity were as follows:pH 5.5 citrate phosphate buffer,40 mmol /L p-NPG solution,incubation at 50 ℃ for 10 min.Vintage Red yeast also showed relative high β-glucosidase activity,higher in extracellular activity than those of the other11 wine yeasts,and with peak production at 36 h growth.
  • ZHANG Lin, CAI Guo-lin, GAO Xian-li, LU Jian, DONG Jian-jun, YU Jun-hong, YANG Mei
    Food and Fermentation Industries. 2014, 40(10): 43-48. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.021
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    After blade grinding and screening pretreatment,the undersize brewer's spent grain was dealt with ball milling.Consequently,the content of crude protein was increased up to 38.8%,which was 36.1% higher than that in origin sample,while the content of soluble protein was up to 2.53%,which was 3.1 times higher than that in origin sample.The soluble protein content of undersize brewer's spent grain was increased up to 12.85% after the enzymatic hydrolysis of neutral protease,which was 1.6 times higher than that in origin sample(7.88%).The results showed that the efficiency of enzyme hydrolysis had been significantly improved by mechanical pretreatment.The enzyme hydrolysis conditions were optimized by single factor experiment and uniform design.The optimal conditions were as follows:the dosage of enzyme of 2400 U /g,enzyme hydrolysis temperature of 45 ℃,enzyme hydrolysis pH of8.0,and enzyme hydrolysis time of 8 h.Under these conditions,the content of soluble protein was up to 21.31%.The exaction rate was up to 54.9%.The hydrolysis liquid was dealt with vacuum freeze drying to prepare feed protein peptide.The crude protein content of feed protein peptide was up to 78.8%.The amino acids content was increased to 74.6%.Essential amino acids content was 28.18%,and polypeptides content was 60.8%.
  • LIU Jun-mei, WANG Hong-ling, WANG Ji-zhi, FAN Chun-yan, WU Jie
    Food and Fermentation Industries. 2014, 40(10): 49-53. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.052
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    Corynebacterium glutamicum was cultivated in two parallel 10 L fermenters under 5.0 m3/h and 3.5m3/h ventilation,respectively.The fermentation conditions of batch cultivation were investigated systematically.The experiment result showed that pH value decreased sharply when the ventilation was 5.0 m3/h.pH value was higher under 5.0 m3/h of ventilation than that under 3.5 m3/h at the end of the fermentation.High concentration of dissolved oxygen fermentation anaphase in fermentation liquid had a significant influence on DO.The L-tryptophan yield and the weight of dried cells reached 43.3 g /L and 18.3 g /L while the ventilation was 5.0 m3/h.Fermentation residual sugar did not change significantly.
  • WAN Hong-gui, DENG Chun-ya, TAN Hai-tao, GONG Yin-cong
    Food and Fermentation Industries. 2014, 40(10): 54-57. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.011
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    The effects of oxidation stimulation and hypertonic stimulation on the production of GSH by waste beer yeast were studied in this research.These two kinds of stimulations were proved to effectively increase the production of GSH.In the oxidation stimulation,when 0.012 g /L of KMnO4 was added after fermentation for 21 hr,the production of GSH was 512 mg /L,which was increased 20% compared to that of control.When 30 mmol /L of H2O2 was added after fermentation for 12 h,the yield of GSH was highest.15 g /L KCl was added after 15 h of fermentation to get a higher production of GSH.The combination of these two kinds of stimulations showed no superimposed effect and less effective than using either of them alone.
  • HU Xiang-dong, PAN Ling-yan, ZHANG Qi, YE Mao, LIANG Xin-le
    Food and Fermentation Industries. 2014, 40(10): 58-62,68. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.053
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    Herein,Phaffia rhodozyma wild-type strain(P.rhodozyma 02) was used as original strain to carry out two rounds of ultraviolet(UV)-LiCl and60Co-γ mutagenesis breeding successively.By using YM medium with 40μmol/L diphenylamine as screening agent,an astaxanthin high-yielding P.rhodozyma mutant strain H4-4 was screened,which was genetically stable and named as HSC-125.The volumetric productivity of astaxanthin of H4-4was 25.36 mg /L,which was 5.34 times higher than P.rhodozyma 02(4.75 mg /L).Two rounds of UV-LiCl and60Co-γ mutagenesis with diphenylamine as selection factor showed good results of breeding high astaxanthin-producing and genetically stable phaffia rhodozyma strains.
  • QI Xi-guang, LI Xiu-fen
    Food and Fermentation Industries. 2014, 40(10): 63-68. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.054
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    Anaerobic acidogenesis provides a novel pathways to reutilize soy sauce residue.The results showed that the efficiency of anaerobic acidogenesis was improved along with decreasing F /M.When F /M was 1 ∶ 1,more VFA,especially acetate,was produced from soy sauce residue.The degradation rates of soluble proteins and carbohydrates were highest with 71.23% and 73.43%.The activities of acid-protease,neutral-protease,alkaline-protease and α-amalyse were also highest with 4.116 U /mL,3.812 U /mL,3.049 U /mL and 13.72 ug maltose /min,respectively.The yields of the total organic acids and acetatic acid per unit substrate were 0.17 g /mL and 0.14 g /mL.And,the percentage of acetatic acid in the total VFA amounted to 82.19%.The conversion of soluble proteins at the beginning period of fermentation was due to the catalytic degradation of acid-protease.The fermentation products involved in ethanol,acetatic aicd,propionatic acid,iso-butyratic acid,butyratic acid and valeratic acid.A certain amount of propionatic acid accumulated during the anaerobic acidogenesis.Under 20∶ 1 F /M,the concentration of propionatic acid was highest with around 4.00 g /L.
  • FENG Yun, LI Bian-sheng, ZHOU Hou-yuan, ZHENG Yuan, GUO Wei-bo, LIN Guang-ming, YANG Huan-bin
    Food and Fermentation Industries. 2014, 40(10): 69-75. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.022
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    In this research,the effects of microwave power density(η = 3.31,4.43,5.59 W /g) and pulse ratio(PR = 1,2,3) on the drying properties and quality of broiler chicken wings were studied.The results showed that the drying occurred under constant rate throughout drying period.The moisture content and drying rate had a significant change at different microwave conditions(P < 0.05).The effective moisture diffusivity had significant change at different microwave conditions(P < 0.05).The value of Deff was between 2.71 × 10-7m2/s and 0.84 × 10-7m2/s at the condition of η = 3.31 W /g,PR = 1 ~ 3;between 3.51 × 10-7m2/s and 1.1 × 10-7m2/s at the condition of η= 4.43 W /g,PR = 1 ~ 3;between 4.44 × 10-7m2/s and 1.35 × 10-7m2/s at the condition of η = 5.59 W /g,PR =1 ~ 3.Eight different empirical drying models were compared and based on statistical tests results,the Page model gave the best fits(R2= 0.9958,χ2= 0.0003) and could be used to accurately predict the moisture content of dried broiler chicken wings during intermittent microwave.The calculated specific energy consumption(ES) ranged from13.48 MJ /kg to 17.97 MJ /kg under different microwave condition,and the effect of pulse ratio on specific energy consumption was higher than that of power density.The intermittent microwave processing could reduced LSR and improved the integrity of appearance.The influence of intermittent processing was lower than continuous processing on shear force at the same η.
  • YAN Ting-cai, CAO Sen, LI Jiang-kuo, ZHANG Peng, CHEN Shao-hui
    Food and Fermentation Industries. 2014, 40(10): 76-82. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.045
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    The effect of different concentration wood vinegar of 5% and 10% on postharvest physiology and quality of Liaoning No.1 green walnuts at cold storage temperature((0 ± 0.5) ℃) were studied.The results showed that wood vinegar treatment could better maintain fruit intrinsic storage quality,effectively control the decrease of nuts L*value,moisture content and firmness effectively,obviously delay the rise of nuts browning index,malondialdehyde(MDA) content,polyphenoloxidase(PPO) activity and lipoxygenase(LOX) activity,preferably keep nuts the activity of peroxidase(POD) and catalase(CAT) during later period storage.Moreover,10% wood vinegar treatment had the best freshness-keeping effect of green walnuts during cold storage,maintained good storage quality,and significantly inhibited oxidation and senescence of green walnuts.Therefore,treatment with 10% wood vinegar could provide the best freshness-keeping effect of green walnuts.
  • QIAO Ning, ZHANG Kun-sheng, REN Yun-xia
    Food and Fermentation Industries. 2014, 40(10): 83-89. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.023
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    In order to study the effects of high pressure processing on the characteristics of mung bean protein,four protein isolates including albumin,globulin,gliadin and glutenin were extracted from mung bean using Osbron method.The effect of high pressure processing(from 200 to 800 MPa for 10 and 20min) on UV-vis spectra and thermal stability of four protein isolates were studied.The results indicated that albumin UV absorption peaks under different pressure treatments was at 265 nm.Ten minutes' UHP treatment could enhance the UV absorption of globulin.However,UHP treatment could reduce the UV absorption of gliadin and glutenin.The effects of pressure on thermal stability of four mung bean protein isolates were different.
  • WEI Xiao-yi, LOU Wen-gao, Wang Shen-qiao, BAI Chen, ZHANG Yan-yan, LU Wen-wei, LIU Tian-qi, MA Bin-jie
    Food and Fermentation Industries. 2014, 40(10): 90-96. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.055
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    Lettuce(Lactuca sativa),spinach(Spinacia oleracea),and kinds of green leafy vegetables were exposed to pesicide chlorpyrifos and carbofuran to study the effects of commercial wash methods(water and NaHCO3solution) on the removal of the residues.The response surface method was used to modify the best cleaning mode which involves washing temperature,time,and solution concentration.The results showed that,NaHCO3 solution could significantly lower pesticide of the green leafy vegetables than water,and the best cleaning condition was washing at 42℃for 28 min with 18‰ mass fraction of NaHCO3 solution.The pesticide residue removal rate was up to 73.88%.
  • ZHU Yan-qing, HU Hua-li, GUO Feng, LI Peng-xia, HU Bo-ran
    Food and Fermentation Industries. 2014, 40(10): 97-102. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.056
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    To investigate the influence of different films packaging on postharvest browning of fresh lotus,we studied the browning and related enzyme activity of fresh lotus with two kinds of film packages in low temperature(5 ±1℃) storage.The concentrations of CO2 and O2,browning indexes,the content of malondialdehyde(MDA) and total phenol,the activities of polyphenol oxidase(PPO),peroxidase(POD) and phenylalnine ammonialyase(PAL) were measured.The results showed that all packages could decrease browning indexes and PPO activities.However,the POD activity was not significantly affected by film packaging.P2 can significantly inhibited the increase of MDA content in fresh lotus,and maintain the higher total phenol content.In addition,P2 also can significantly decrease the PPO activity and increase the activity of PAL.In conclusion,P2 had the best effect on inhibiting the browning of fresh lotus.
  • HU Huai-rong, ZHANG You-hua, TANG Ping, XIAN Xin-yan, Li Ming-yuan
    Food and Fermentation Industries. 2014, 40(10): 103-107. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.057
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    Low-salt pickled turnips are easy to be deteriorated caused by microbial.Antibacterial experiments for microbial isolated from low-salt pickled turnips were conducted in order to extend the product shelf-life.Meanwhile,the screening and optimization of preservatives were carried on.An obvious suppression effect of sodium benzoate,sodium dehydroacetate on spoilage microbial was observed.A compound preservative was optimized at 0.4g /L sodium benzoate,0.2g /L sodium dehydroacetate by orthogonal test.This compound preservative exhibited an excellent suppression effect on Lysinibacillus sphaericus,Bacillus sp.The antibacterial rate of the two strains could reach to99.77%,99.86% respectively.
  • GUO Ying, TU Shun-ming, LUO Lian, LIU Xin-zheng, ZHENG Fang-yan
    Food and Fermentation Industries. 2014, 40(10): 108-112. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.058
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    In this paper,Streptococcus thermophilus,Lactobacillus bulgaricus and Lactobacillus plantarum subsp.plantarum were domesticated to adapt to the fermentation environment of 100% pure soybean milk.The optimal production conditions were selected by single-factor experiments and orthogonal experiment.Ratio of three kinds of bacteria was 1∶ 1∶ 1,inoculum amount was 5%,fermentation temperature was at 40 ℃ and fermentation time was 5 h.Under the above optimum conditions,the acidity of fermented soybean milk was 98.7° T,the viscosity was 3875mPa·s,and the total bacterial count was 5.63 × 108 CFU /g.The fermented soybean milk had good curd effect,no whey separation,no bean flavor.The milk was delicate,smooth and white.This research provide technical reference for the development of high quality soy yogurt.
  • TANG Ming-li, WANG Bo, LIU He, HE Yu-tang, HUI Li-juan, MA Tao
    Food and Fermentation Industries. 2014, 40(10): 113-118. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.059
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    Lactic acid bacteria of Fermented pancake batter was identified by VITEC-2 compact microbial identification system and the protective agent for preparation of freeze-dried bacteria ferment was screened and optimized.The results showed that lactic acid bacteria in pancake batter fermentation was pediococcus pentosaceus.Through single factor and response surface methodology of experiments,the formulation of cryoprotectant was Trehalose 1.38%,sodium glutamate 4.80%,sucrose 13.80%,skim milk 13.50%,Under optimized conditions,the survival rate of pediococcus pentosaceus could reach 88%.
  • YANG Xiao-ping, YUE Si-jun, XU Chun-yan, SU Jian-yu
    Food and Fermentation Industries. 2014, 40(10): 119-122. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.014
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    To get the complex fermentation technique of Cordyceps militaris and Lycium barbarum,the growth curve of Cordyceps militaris and the active ingredients contents of complex fermentation product were detected.The optimum fermentation conditions of complex fermentation were obtained as follows:5 g /L of the Lycium barbarum homogenates was introduced into the medium when the fermentation time of Cordyceps militaris was 72 h,and the fermentation period was 144 h.Under optimum conditions,the yield of complex fermentation product reached 11.73 g /L,which was 112.12% higher than that of Cordyceps militari mycelia.The contents of cordycepin,cordyceps acid,and polysaccharides of the complex fermentation product were respectively 11.23 mg /g,178.21 mg /g,and 30.73 mg / g,which were increased by 29.98%,64.46% and 97.20% compared with those of Cordyceps militari mycelia.The antioxidant capacity of complex fermentation product was increased by 44.62% than that of Cordyceps militari mycelia based on the determination of the hydroxyl radical scavenging activity.
  • YAO Min, TAN Shu-ming, ZHANG Shao-cai, LIU Feng-qi, LI Dong, DENG Yi
    Food and Fermentation Industries. 2014, 40(10): 123-127. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.029
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    Rosa roxburghii Tratt dry wine(RRT-DW) made from Rosa roxburghii Tratt juice(RRTJ) was produced in this study.With its alcoholic strength 13.46 °,surge content 3.7 g /L,and acids content 8.37 g /L,it had good properties and typical Rosa roxburghii Tratt flavor.Effective compound in Rosa roxburghii Tratt dry wine and Rosa roxburghii Tratt juice were detected,such as amino acids,alcoholic strength,surge content,total acid,pH value,vitamin C,polyphenols,flavonoids.The results showed that there were 11 types of amino acids in Rosa roxburghii Tratt dry wine,5 of them were essential,and contained 52.71 % of amino acids from Rosa roxburghii Tratt juice.The acids of Rosa roxburghii Tratt dry wine decreased 2.76 g /L,compared with those of Rosa roxburghii Tratt juice,and the rang of pH value was from 3.52 to 4.10.Vitamin C contained 73.48 % of its content in Rosa roxburghii Tratt juice,and polyphenols contained 73.45 %,flavonoids contained 87.54 %,which were relatively stable during the fermentation process.
  • WANG Su-ya, HU Dan-dan, YANG Xiao-ya, JU Xin-grong
    Food and Fermentation Industries. 2014, 40(10): 128-132. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.060
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    After immersion 6h in 10 mg /L Na2SeO3 solution,soybean was germinated 96 h at optimal conditions.Soybean protein isolate(SPI) was obtained from germinated soybean with alkaline extraction and acid precipitation method.After adding GDL,soy protein gels were analyzed by Texture Analyzer and the impact of Se and the germination time on gel property was investigated.The results showed that the hardness of soybean gel and SPI gel decreased rapidly from 25.25 g to 10.77 g and from 27.73 g to 13.37 g respectively during 48 h germination.Water holding capacity(WHC) of soybean gel and SPI gel decreased from 61.42% to 51.55% and from 62.64% to 55.54% respectively.The changes of SPI during germination were discussed by SDS-PAGE mapping.The results showed that β-7S subunit and B-11 S subunit were relatively stable,while α'-7S,α-7S,A3-11 S and A-11 S subunits were easily hydrolyzed by intra-proteases.At the same germination time,the protein bands of se-enriched soybean were similar with the control group.It showed that enriched selenium treatment had a little effect on gel property of soybean protein during germination.
  • SHI Yan-chang, DU Jin-suo, CHEN Jian, LI Miao-miao, WANG Chun-hua, ZHU Yong-zhen, WANG Xiao-gang
    Food and Fermentation Industries. 2014, 40(10): 133-136. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.061
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    In this paper,the effect of ozone disinfection process on xanthan gum fermentation sterilization was studied,and was compared with the traditional steam sterilization method.The results showed that the residual ozone of ozone sterilized water could be cleared after incubation at pH 8.5,30 ℃ for 15 min.The ozone sterilized water could be better applied in fermentation of xanthan gum to improve the quality of products.Compared with conventional steam sterilization,ozone sterilization was more conducive to reduce energy consumption of xanthan gum fermentation.
  • ZHAI Fei-hong, WANG Lin-lin, HAN Jian-rong
    Food and Fermentation Industries. 2014, 40(10): 137-141. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.062
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    The aim of this work was to evaluate the antioxidant activity and polysaccharides content in mycelial pellets of Agaricus blazei and Agaricus bisporus by liquid state fermentation.The EC50 values of Agaricus blazei in scavenging effect on DPPH radical,reducing power,Ferrous Ions' chelating capacity and superoxide anion's scavenging capacity were respectively 4.16 mg /mL,8.28 mg /mL,6.54 mg /mL and 6.16 mg /mL.The EC50 values of Agaricus bisporus in scavenging effect on DPPH radical,reducing power,Ferrous Ions' chelating capacity and superoxide anion's scavenging capacity were respectively 2.56 mg /mL,22.48 mg /mL,14.06 mg /mL and 7.16 mg /mL.These results showed that the antioxidant activity in mycelia pellets of Agaricus blazei was superior to that of Agaricus bisporus.The polysaccharide content of Agaricus blazei was 9.85%,while the Agaricus bisporus was 8.42%.
  • WANG Bi-ni, HUANG Qing-yuan, GAO Hui, CHENG Ni, CAO Wei
    Food and Fermentation Industries. 2014, 40(10): 142-145. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.063
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    Organic solvents with different polarities were used to extract jujube polyphenols with high antioxidant activity.The extraction rates,total phenolic contents and antioxidant activities of the obtained extracts were compared.The results indicated that liquid-liquid extraction was a successful method for separating jujube polyphenols with different polarities.The stronger the polarity of organic solvent is,the higher the extraction rates,total phenolic contents and antioxidant activities of the extracts are.Most jujube polyphenols with high antioxidant activity are polar phenols.Therefore,weak polar polyphenols should be removed from crude extracts to achieve high antioxidant activity extract.
  • JIANG Bao, LUO Mei-juan, ZHANG Xiao-zhuan, ZHANG Zhen-wen
    Food and Fermentation Industries. 2014, 40(10): 146-150. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.064
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    To understand the phenolic compounds and antioxidant activities of Cabernet Sauvignon and Merlot berries,providing some theoretical evidence for wine-grape from four different region(Yuquanying of Ningxia,Xiangning of Shanxi,Changli and Shacheng of Hebei),the physicochemical index,the phenolic content,antioxidant activities and individual anthocyanin concentration of two kinds of grapes were analyzed.The results showed that the contents of total phenolic,flavonoids,flavanols and anthocyanins compounds and antioxidant capacity of Yuquanying grape were the highest,Shacheng of Hebei took the second place,the other two regions were varied;the total 22 and23 individual anthocyanin compounds from Cabernet Sauvignon and Merlot berries were identified,including 5 basic anthocyanins and 18 individual anthocyanin deriatives;effect of ecological condition on individual anthocyanin composition mainly reflected in anthocyanin deriatives,and the grape variety factor had significantly effect on concentration of individual anthocyanin compounds.So the ecological condition of territory could influence the phenolic content and anthocyanin composition.
  • HAN Lin, DING Bo, WANG Zhao-dan, WANG Kai-tuo, ZHENG Hong, ZHENG Hai-ying
    Food and Fermentation Industries. 2014, 40(10): 151-155. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.065
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    In order to research the effects of hydrolysis time and proteases types on functional properties and antioxidant activity of the hydrolysates from Monopterus albus meat,Papain,Alkaline Protease,Trypsin and their two combinations were used to hydrolyze the Monopterus albus meat under their optimum conditions,and different degrees of hydrolysis were obtained,meanwhile,their functional properties and antioxidant activity were tested.The results showed that,with the prolonging time of hydrolysis,the antioxidant activity of hydrolysates from Monopterus albus meat were increasing.The best radical scavenging for DPPH and ABTS were 62.18% and 81.76%,respectively.However,the radical scavenging activity of the hydrolysates were decreased with the further prolonging time of hydrolysis.The hydrolysates obtained by two enzymes showed better antioxidant activity,solubility and heat stability than those obtained by single enzyme,but were worse in emulsibility.The functional properties of the hydrolysates were affected greatly by the hydrolysis time.
  • FAN Qiao, CHEN Hou-rong, XU Xiao-ping, TIAN Mei-ling, ZHANG Fu-sheng
    Food and Fermentation Industries. 2014, 40(10): 156-161. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.066
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    The combination of proteins and polysaccharides showed the excellent properties than they used alone,it affects flavor,nutrition,and texture of food system.Now,the research of polysaccharides /protein complexes is becoming a hot-spot for investigators in the world.By using konjac glucomannan as a representative polysaccharides,the physical,chemical and biological modification of konjac glucomannan /protein complexes as well as the change of modified composite' function were reviewed and the modified mechanisms were also discussed in the hope of providing a reference for other polysaccharide /protein complexes.
  • ZHAO Ting, YAO Su, XU You-qiang, CHENG Chi
    Food and Fermentation Industries. 2014, 40(10): 162-167. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.067
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    Bacterial antimicrobial resistance(susceptibility to antimicrobials) is a hot problem internationally and domestically.Based on the European Food Safety Authority relevant regulations,this article introduces assessment and supervision system of bacterial susceptibility to antimicrobials in food and feed industry,including MIC method and molecular gene method from phenotype to genotype,which provides some references for bacterial susceptibility to antimicrobials assessment in food and feed industry of China.
  • ZHAO Jing, QI Yan, WANG Yue-hua, FENG Xu-qiao
    Food and Fermentation Industries. 2014, 40(10): 168-174. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.068
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    In this review,technological advances on isolation and purification of phycocyanin and their application were summarized from detailed introduction to the purified methods such as mutli-step salting out,aqueous twophase extraction,salting out combined with aqueous two-phase extraction,reversed micellar extraction,chitosan affinity precipitation-activated carbon adsorption-DEAE Sephadex A-25 column chromatography,combined ion exchange chromatography and hydrophobic interaction chromatography.In addition,factors that influence the effect of these methods were discussed.The present problems related to the isolation and purification were analyzed and future application of phycocyanin was proposed.
  • YING Jian, HAO Bin-xiu, WANG Li-ming, WANG Chun-ling
    Food and Fermentation Industries. 2014, 40(10): 175-179. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.069
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    Honey has a long history used as food and medicine.It has a pleasant taste and extensive physiological activities.Heating,filatration and concentration were common steps of modern honey processing to sterilize honey and suppress crystalization.Physical and chemical properties of honey could change during processing;nutritional value and functional activities could also be affected.To provide references for production of high quality honey,the effects of honey processing toward product quality were reviewed from the perspective of the appearance,taste,composition,shelf-life and biological activity.The most recent advances on honey processing were introduced.
  • HE Hai-fen, YAN Jie, LIN Hai-lin, LIU Qi-hai
    Food and Fermentation Industries. 2014, 40(10): 180-183. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.039
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    The usage of soy sauce residue has a great value,but few applications are available.The methods of extracting crude fat from soy sauce residue including squeezing methods,single solvent extraction,mixed solvent extraction and extraction assisted by ultrasound were introduced.On the basis of preliminary research,taking characteristics of soy sauce residue into account,all theoretical analysis and operational feasibilities of these methods are reviewed seriously.It also points out that removing the salt and water quickly and efficiently from fresh soy sauce residue is the bottleneck problem of the current? research.An ideal method with acetone as solvent is worth of further studying.
  • WU Shuai, YANG Xi-hong, XIE Wan-cui, YANG Ya-dong, FENG Xian
    Food and Fermentation Industries. 2014, 40(10): 184-188. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.070
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    Research progress on new technology for fermentation of fish sauce and improvement of its flavor.Fish sauce is a kind of natural fermentation seasoning produced with low-value fish,shrimp or fish by-products.Its production has a long history and tradition because of its delicious taste and nutrition.In this paper,the specific compounds related to the taste and aroma of fish sauce were outlined,and the mechanism of flavor formation was analyzed.The traditional fish sauce fermentation technology and new fermentation technologies were introduced according to the main factors affected the flavor,then the characteristics and shortcomings of these two types of fermentation technologies were pointed out.The effects of different fermentation technologies on fish sauce flavor,the microorganisms and enzymes improving the flavor of fish sauce and the deodorization of fish sauce were discussed.Finally,the outlook of fish sauce as the industrial fermented seasoning was prospected.We hope it could provide some reference for future research.
  • AI Dui, ZHANG Fu-xin, LI Yan-hua, SU Wei-li, LIU Ling, YU Ling-ling, GE Ping
    Food and Fermentation Industries. 2014, 40(10): 189-193. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.071
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    The volatile compounds of the goat milk and cow milk were extracted by head space solid phase micro extraction(HS /SPME),separated and identified by gas chromatography-mass spectrometry(GC /MS).The results showed that 45 compounds were obtained from goat milk,mainly consisting of acids,hydrocarbons,ketones,and aromatic.33 compounds were obtained from cow milk,mainly consisting of acids,hydrocarbons,alcohols,and ketones.Nineteen of them were the same components including acids(7 kinds),hydrocarbons(4 kinds),aromatic(3kinds),esters(2 kinds),ketones(2 kinds),alcohols(1 kinds),aldehydes(1 kind),and other compounds(2kinds).Volatile component of goat's milk was more complex than that of cow's milk,especially in volatile acids.
  • WU Su-su, ZHAO Gai-ming, LIU Yan-xia, TIAN Wei, SUN Ling-xia
    Food and Fermentation Industries. 2014, 40(10): 194-199. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.072
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    In order to analyze the forming process of the flavor substances in chicken during cooking.Chicken thighs were selected as the study material.The amount of the spice was doubled that of the Daokou Roasted Chicken.Chicken thighs were divided into eight groups and weighed at 1.80 ± 0.20 kg /each group.Then chicken thighs were all put into a covered pot.The electric oven(a total of six grades and maximum power of 2 kW) was set at grade 5 to cook the meat till the temperature of water reached 95 ℃.Then chicken thighs were keep in 95 ± 2 ℃ for 10,20,30,40,50,60,90,120 min respectively.The lost water due to evaporation was not added.Electronic nose(EN)and Headspace solid-phase micro-extraction(HS-SPME) combined with Gas chromatography-Mass spectrometry(GCMS) were adopted to detect flavor compounds and three-parallel experiments were conducted.The results were performed by principal components analysis(PCA).In different groups,56,54,54,62,60,63,61 and 58 kinds of volatile compounds were identified successively;the characteristic components in each group were as follows:10 min(benzene;a-Terpineol),20 min(hexane;2-butanon e,3-hydroxy-),30 min(botanic acid,butyl ester),40 min(2-decenal,(E)-),50 min(pentane),60 min(styrene;benzene,methyl-;2-hexenal,(E)-;2-propenoic acid,butyl ester;pentanal),120 min(limonene);The data areas of EN dispersed according to certain rules:areas of 10,20,30 min could be easily set apart from each other;areas of 40,50,60 min were next to one another;areas of 90,120 min had a substantial overlapping portion.The results indicate that the relative content and kinds of volatile compounds in the chicken thighs varies over the time.At the initial stage,the overall flavor of chicken thighs changes quite rapidly,and then the changes slowed down gradually.After cooking for 90 min or more,little changes were occurred.
  • ZHAO Xiang-ying, ZHANG Li-he, YANG Li-ping, HAN Yan-Lei, TIAN Yan-Jun, ZHANG Jia-xiang, LIU Jian-jun
    Food and Fermentation Industries. 2014, 40(10): 200-204. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.073
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    The earlier investigation indicated that,in addition to erythritol,the erythritol mother liquor still contained other unknown sugar alcohol constituents.In this paper,the high performance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS) analysis were employed in order to qualitatively determine these unknown substances.To be first,the HPLC analysis was undertook using the NH2 column and Shodex SC1011 column,respectively.Based on the comparison between the mother liquor peaks and standard samples peaks,the preliminary conclusions were drawn that the unknown constituents were mainly arabitol and mannitol,as well as a few amounts of ribitol.Furthermore,the LC-MS analysis revealed that their molecular weights were coincided with that of arabitol,mannitol and ribitol.Altogether,the results provided a scientific basis for the further resource utilization of the mother liquor.
  • XU Rui, TAN Hong, YANG Hong-bo, SUN Hai-da, HE Jin-lin
    Food and Fermentation Industries. 2014, 40(10): 205-209. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.074
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    A method was established for the determination of 7 perfluorinated compounds(PFCS) in four kinds of food wrapping materials(popcorn bucket,barbecue oiled papers,hamburger bags and egg tart tinfoil) by solid phase extraction coupled with liquid chromatography-tandem mass spectrometry(SPE-LC-MS /MS).The results showed that four kinds of PFCs including Perfluorohexanoic acid(PFHxA),perfluoroheptanoic acid(PFHpA),perfluorooctanoate acid(PFOA) and perfluorononanoic acid(PFNA) were detected in popcorn bucket and their average concentrations were 15.8114,0.4690,0.2225 and 0.0363 μg /kg,respectively;PFHxA was detected in barbecue oiled papers,and the average concentrations was 1.3344 μg /kg;PFHxA and PFHpA were detected in hamburger bags,and their average concentrations were 0.1639 and 0.4599 μg /kg;there was a different pollution degree between 3batches of egg tart tinfoil.PFHxA was detected in both batch 1 and 2,the average concentrations were 1.461 and0.1770 μg /kg,respectively;however six kinds were detected in bach 3 including perfluorochemicals including perfluorohexanoic acid,perfluoroheptanoic acid,perfluorooctanoate acid,perfluorododecanoic acid(PFDoA),perfluorotetradecanoic acid(PFTA) and perfluorooctane sulphonate(PFOS),and the average concentrations were 1.68,3.15,0.68,0.74,0.64 and 0.07 μg /kg,respectively.The limits of detection for the method(LODS),average recoveries and RSD for repeated measurements(n = 6) were 0.03 ~ 0.52 μg /kg,88% ~ 110% and 1.7% ~ 5.0%,respectively.The method was simple,accurate and sensitive.It was suitable for the determination and confirmation of PFCs in different kinds of food wrapping materials.
  • LI Huan, SHI Long-mei, ZHOU Jian-ke
    Food and Fermentation Industries. 2014, 40(10): 210-212. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.075
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    Subzero-temperature frozen extraction technique which using acetonitrile as a solvent was applied to the extraction of pesticide such as Tribenuron,Primisulfuron,Hexaflumuron,Teflubenzuron,Triflumuron from flavored milk drinks.HPLC was used for the determination.The pesticide were separated by a reversed-phase C18 column and detected by Variable wavelength UV.The mobile phase was the mixture of methanol and water and the volume ratio was 73:27.Acetonitrile was used in extracting under 18 degrees below zero.Salt effect,frozen time and the volume ratio of extraction agent on the effect of extracting efficiency were studied.Under the optimal conditions,the linear regression equations of five pesticide was good and the correlation coefficient was more than 0.9994.The relative standard deviations were less than 2.333%.The recovery rates of the target analyte were between 92.3% and 98.7%.
  • ZHANG Rui, LUO Xin, ZHU Li-xian, SUN Bao-zhong, HAN Ming-shan
    Food and Fermentation Industries. 2014, 40(10): 213-218. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.076
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    In order to study the effects of high pressure processing on the characteristics of mung bean protein,four protein isolates including albumin,globulin,gliadin and glutenin were extracted from mung bean using Osbron method.The effect of high pressure processing(from 200 to 800 MPa for 10 and 20min) on UV-vis spectra and thermal stability of four protein isolates were studied.The results indicated that albumin UV absorption peaks under different pressure treatments was at 265 nm.Ten minutes' UHP treatment could enhance the UV absorption of globulin.However,UHP treatment could reduce the UV absorption of gliadin and glutenin.The effects of pressure on thermal stability of four mung bean protein isolates were different.
  • YANG Wan-gen, XIE Qiong, ZHU Qiu-jing, GU Ren-yong, HUANG Qun, YU Ji
    Food and Fermentation Industries. 2014, 40(10): 219-222. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.077
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    Qianzhou dry-salted duck is a traditional cure meat product.To investigate the degradation law of the proteins of the duck during processing,we used SDS-PAGE to analyze the degradation of the duck breast at different processing phases.The change of muscle fiber was investigated by scanning electronic microscopy.The results showed degradation was very obvious in whole muscle proteins,sarcoplasmic proteins and myofibrillar protein on day 14.During the period of 21 th day of curing to 14 th day of air-drying,the degradation of proteins was not obvious.Tight muscle fibers could be observed at 14 th day of curing.But it became loose with the muscle fibers reducing and fractured after the 7th day of air-drying.When the duck was air-dried for 14 days,the interspace among the muscle fibers were getting bigger,so the amount of muscle fibers reduces.The investigation provides scientific guidance of modification to the process of traditional Qianzhou dry-salted duck production.
  • WANG Ming-hui, YAN Jia, HAN Yong-bin, GU Zhen-xin
    Food and Fermentation Industries. 2014, 40(10): 223-228. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.078
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    In order to optimize the parameters for the aqueous ethanol extraction of Melanoidin from Chinese traditional lotus roots filled with sticky rice,the single factor and L9(34) orthogonal experimental design were applied.The results showed that the extraction temperature was the principal factor,followed by ethanol concentration,extraction time,and solid-liquid ratio.The optimum extraction condition was that the grinded lotus roots was immersed into10% ethanol water solution with a ratio of 1∶ 40(w /v),maintaining for 1.5 h at 80℃.Under this condition,AV value of extraction of Melanoidin reached 8.325.The antioxidant activities of Melanoidin in the extracts were estimated by scavenging capacities of a 2,2-diphenyl-picrylhydrazyl radical(DPPH·),hydroxyl radical(·OH) and reducing power under different concentrations.We found that the antioxidant activities of three indexes significantly increased(P < 0.05) with the increasing concentration of Melanoidin,and when the relative concentration was 1.0,the three indexes of antioxidant activities reached to 53.39 %,10.16 % and 15.95 %,respectively.The extracts DPPH· scavenging capacity was the highest,followed by reducing power and ·OH scavenging capacity.It was concluded that Melanoidin in Chinese traditional lotus roots filled with sticky rice had of good antioxidant activity and has a dose-effect relationship.
  • XU Wei, KE Zeng-guang, YAN Xiu-hua, SHAO Rong, YUN Zhi
    Food and Fermentation Industries. 2014, 40(10): 229-233. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.079
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    The microwave-assisted extraction of ricinine from castor meal was studied by single factor experiment and response surface methodology.The antibacterial activity of ricinine was also investigated by filter paper method.The optimum extraction parameters of ricinine were as follows:microwave power 408.01 W,extraction time 5.21 min and ratio of liquid to solid 12(mL∶ g).Under the above conditions,the predicated extraction rate was 50.74 %,and the actual extraction rate was 50.81 %.The relative deviation was 0.14 %.There was no significant difference between predictive value and actual value.The antibacterial activity of ricinine showed that ricinine had inhibition capacity to all tested strains,and the inhibition order was:Escherichia coli > Bacillus amyloliquefaciens > Staphyloccus aureus.
  • WAN Hong-gui, TAN Hai-tao, DENG Chun-ya, GONG Yin-cong, XUE Cai-li
    Food and Fermentation Industries. 2014, 40(10): 234-237. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.080
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    A feasibility study on extracting riboflavin from fermentation by spiral-wound membranes with molecular weight cut-offs(MWCO) of 1000 Da,10 kDa and 30 kDa was explored.The membrane with MWCO of 10 kDa was selected to have the best separation effect through comparing the efficiency and flux of the membrane.After the suitable membrane was ascertained,the effects of various parameters for operation such as pressure,flow rate,temperature,concentration factor were studied.The optimal operating conditions were obtained as follows:operation pressure0.2 MPa,operation temperature 30 ℃,flow rate 5 m /s,concentration factor 3.8.The riboflavin purity could reach98.6 % and total recovery rate was up to 97% after ultrafiltration and crystallization
  • SUN Yan, GUO Qing-xing, TONG Qun-yi
    Food and Fermentation Industries. 2014, 40(10): 238-244. https://doi.org/10.13995/j.cnki.11-1802/ts.2014.10.081
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    Flavonoids were extracted from Brassica napus L.bee pollen by ultrasonic-microwave synergistic method.The effects of alcohol concentration,extraction time,liquid-solid ratio,and microwave power on the extraction yield of total flavonoids were studied.The optimal extraction conditions determined through single-factor and orthogonal experiments were as follows:alcohol concentration 90%,liquid-solid ratio 30∶ 1,microwave power 260 W and extraction time 180 s.Under the above conditions,the extraction yield of total flavonoids was up to 3.36%.Compared with hot reflux extraction,ultrasonic and microwave extraction,ultrasonic-microwave method had obvious high efficiency.Moreover,ultrasonic-microwave method had the advantages of low energy consumption and safe.LC-MS analyses exhibited that four kinds of flavonoid aglycones were identified in the ultrasonic-microwave synergistic extraction of Brassica napus L.bee pollen:Quercetin-3-O-β-D-pyranoid glucose base-(1 → 2)-β-D-pyranoid glycosidase,Kaempferol-3,4 '-double-O-β-D-pyranoid glycosidase,Isohtamnetin-3-O-β-D-pyranoid glucose base-(1 →2)-β-Dpyranoid glycosidase and Kaempferol-3-O-β-D-pyranoid glucose base-(1→2) β-D-pyranoid glycosidase.