RAN Yonglan, KAN Jianquan
To explore the expression regulation of differential genes in vegetables under the stress of diethylhexyl phthalate (DEHP), observation of the submicroscopic structure of radish roots was conducted using transmission electron microscope, also, analysis of the response transcriptome by RNA-Seq technology for radish treated by different concentrations (10, 30 and 50 mg/kg) of DEHP was applied. The results indicated that DEHP stress destroyed the structure of radish root cells, with thickened, deformed or partially fractured cell walls, and with less mitochondrial cristae as well as blurring and even disintegrating internal cristae. Higher concentration of DEHP led to more serious damage. In the analysis of response transcriptome, 52 707 576, 47 261 670 and 48 651 920 Clean Reads were obtained in three contaminated groups, which generated 9 885, 9 385 and 11 880 differentially expressed genes, respectively. Gene Ontology (GO) analysis showed that the differentially expressed genes were mainly concentrated in the biological processes of metabolism, stimulus response and catalytic activity. And Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the differentially expressed genes were mainly involved in the metabolism of phenylalanine, linoleic acid, α-linolenic acid, glycine, serine, threonine, cysteine and methionine, biosynthesis of phenylalanine, tyrosine, tryptophan, phenylpropionic acid and carotenoid. Alanine-glyoxylate transaminase, spermine synthase, lipoxygenase, acetyl-CoA C-acetyltransferase participated in the stress response of radish through metabolism of cysteine and methionine, linoleic acid as well as α-linolenic acid. To sum up, these data are helpful for revealing the mechanism and molecular basis of radish roots under DEHP stress, and for mining relevant resistance genes and molecular-assisted breeding.
