15 December 2020, Volume 46 Issue 23
    

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  • LI Zhiqiang, LIU Ying, LIN Feng, WU Liyun
    Food and Fermentation Industries. 2020, 46(23): 1-6. https://doi.org/10.13995/j.cnki.11-1802/ts.025039
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    In this study,the citrinin content of fermented rice produced by 22 strains from three species ofMonascus were analyzed using high performance liquid chromatography with fluorescence detection,and two pairs of primers were used to amplify the citrinin biosynthesis related genes of these strains by polymerase chain reaction.The correlation between citrinin production and the key genes responsible for citrinin biosynthesis in these strains was investigated.All 11M.purpureus strains and fourM.ruber strains could produce citrinin,and the amplification of bothpksCT andctnA was positive.The amplification ofpksCT was positive,while the amplification ofctnA was negative in the other sixM.ruber strains with the phenotype of undetectable citrinin production.A single-base deletion was observed in the amplified pksCT coding regionsof M.sanguineus R18,and bothctnA amplification and citrinin detection were negative.It was indicated that the simultaneous presence of functionalpksCT andctnA genes was highly related to citrinin production amongM.purpureus,M.ruber andM.sanguineus.
  • WANG Shuai, ZHUANG Yibin, LIU Hao, BI Huiping, LIU Tao
    Food and Fermentation Industries. 2020, 46(23): 7-13. https://doi.org/10.13995/j.cnki.11-1802/ts.024788
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    Escherichia coli co-culture was designed for biosynthesis of caffeyl alcohol glucosides from glucose.Three caffeyl alcohol monoglucosides,namely caffeyl alcohol-3-O-glucoside,caffeyl alcohol-9-O-glucoside and caffeyl alcohol-4-O-glucoside,were produced by co-culturing two recombinantE.coli strains.A biosynthetic pathway ofp-coumaryl alcohol was constructed in the high-tyrosine-producingE.coli BTAL-CAD01 by expressing tyrosine ammonialyase,hydroxycinnamate:CoA ligase (4CL),and cinnamyl-CoA reductase fromRhodotorula glutinis,Petroselinum crispum,andArabidopsis thaliana,respectively,together with endogenous alcohol dehydrogenases or aldo-keto reductases.Then,4-hydroxyphenlacetate 3-hydroxylase (HpaBC) fromE.coli and glucosyltransferase UGT73C5 fromA.thaliana were co-expressed in wild-typeE.coliBL21 (DE3),resulting in a recombinant strain BWT-CAD01.Caffeyl alcohol monoglucosides were successfully produced by co-cultivating the recombinant strains BTAL-CAD01 and BWT-CAD01.The highest titer of caffeyl alcohol-4-O-glucoside reached (141.63±3.42) mg/L,with optimized inoculation ratio of 3:1.To our knowledge,this is the first report on heterologous biosynthesis of caffeyl alcohol glucosides,both caffeyl alcohol-3-O-glucoside and caffeyl alcohol-9-O-glucoside are unnatural compounds.This work expands the structural diversity of caffeyl alcohol glucosides,which makes it possible the discovery of new analogues with improved pharmaceutical properties.
  • LI Xinyu, SUN Yulu, XU Yan, ZHANG Rongzhen, TANG Ke
    Food and Fermentation Industries. 2020, 46(23): 14-20. https://doi.org/10.13995/j.cnki.11-1802/ts.024808
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    Three different aging containers (stainless steel tank,Chinese oak barrel and French oak barrel) were used to ageAronia melanocarpa (Michx.) Elliott wine.The changes of wine samples in different aging containers during aging process were studied by measuring the mono-phenol content and antioxidant capacity (ABTS,DPPH,FRAP) of wine samples.With the aging time,the total phenol content decreased by 14.53%,27.67% and 25.89%,respectively;and the total anthocyanin content decreased by 58%,71% and 74%,respectively.The changes of antioxidant capacity were similar to that of total phenol and anthocyanin content.The total antioxidant capacity of ABTS decreased by 17.16%,30.83% and 24.78%,correspondingly;free radical scavenging capacity of DPPH decreased by 18.31%,25.35% and 22.54%,while reduced iron capacity of FRAP decreased by 60.15%,74.05% and 52.80%,respectively.According to the correlation analysis,the correlation of these five indicators was significant.The determination results of mono-phenol showed that the content of chlorogenic acid,epicatechin and rutin decreased with the aging time.The contents of ellagicacid and protocatechuic acid increased with the aging time.This study provides basic theories for the in-depth study of the mechanism of antioxidant changes in the aging process ofA.melanocarpa (Michx.) Elliott wine,and it also has practical significance to regulate the aging process ofA.melanocarpa (Michx.) Elliott wine.
  • Dingding, HUANG Mengyi, ZHENG Huina, CAO Wenhong, LIN Haisheng, QIN Xiaoming, ZHANG Chaohua
    Food and Fermentation Industries. 2020, 46(23): 21-28. https://doi.org/10.13995/j.cnki.11-1802/ts.024605
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    In order to develop bioproducts from abalone (Haliotis discus hannaiIno),abalone muscle was enzymatically hydrolyzed and the immunoactivity of the hydrolysate was analyzed.Animal protease and flavor protease preparations were used for enzymolysis of abalone muscle,and protein recovery rate and degree of hydrolysis (DH) were used as indexes for optimization of the enzymolysis conditions.The immunoactivity of enzymatically hydrolyzed peptides (AHP) was analyzed with zebrafish model.The results showed that optimal protein recovery and DH were obtained after 4h hydrol-ysis,which were (55.88±0.03)% and (20.32±0.01)%,respectively.The results ofin vitro immunoactivity experiments showed that AHP increased the activities of lysozyme (LYS) and alkaline phosphatase (ALP) and level of IgG in zebrafish model,of which the activity of LYS and ALP reached (166.04±23.48) U/L and (7.46±1.02) IU/L,respectively,and the IgG reached (0.31±0.09) μg/mL in the freeze-dried sample group,which were significantly different from that of the control group (P<0.05).Among the ultrafiltration components,compared with the >10 kDa and 5 k-10 kDa sample groups,the activity of the <5 kDa ultrafiltration sample group was significantly higher (P<0.05),the highest activity of LYS and ALP were (1481.29±160.70) U/L and (1 617.39±206.70) IU/L,respectively,and IgG was up to (34.77±2.40) μg/mL.AHP exhibited certain immunoactivity and the <5 kDa group ultrafiltration components were the most effective.
  • ZHANG Meiyi, ZHAI Qixiao, ZHAO Jianxin, ZHANG Hao, CHEN Wei
    Food and Fermentation Industries. 2020, 46(23): 29-34. https://doi.org/10.13995/j.cnki.11-1802/ts.024742
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    This study was conducted to investigate the effect ofLactobacillus rhamnosus JS-SZ-2-1 againstHelicobacter pyloriinfection.L.rhamnosusstrains were tested for their ability to inhibit the growth ofH.pylorias well as to reduce the adhesion ofH.pylorito gastric epithelial cellin vitro,and then the clinical effect was further investigated.Twenty-sixH.pylori-positive patients were recruited and randomly divided into 2 groups:placebo group andL.rhamnosus JS-SZ-2-1 group.Probiotic powder was given twice a day to volunteers for one month and then the indicators were tested.In vitro results showed thatL.rhamnosus JS-SZ-2-1 significantly inhibited the growth ofH.pylori and the adhesion ofH.pylori to AGS.Compared to placebo group,Lactobacillusstrains can alleviate H pyloriinfection by reducing the value of 14C-urea breath test (UBT,△UBT=106.00) and gastrointestinal symptom rating scale (GSRS,P<0.05).Besides,there was a significant alteration in the structure of intestinal flora,while no significant changes were found in the serum levels of pepsinogen,IL-8 and TNF-α.No adverse reactions occurred in the process of probiotic intervention.These results suggested thatL.rhamnosus JS-SZ-2-1 may alleviate H.pyloriinfection and improve the value of GSRS.
  • YIN Mingyu, LIU Qijun, ZHANG Zongyi, TANG Shijie, RYOSUKE MATSUOKA, XI Yinci, WANG Xichang
    Food and Fermentation Industries. 2020, 46(23): 35-40. https://doi.org/10.13995/j.cnki.11-1802/ts.024938
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    To explore the effects of egg yolk phospholipid and soybean phospholipid in regulating the glycolipid metabolism of the body during multiple traumatic blood loss processes within 24 hours,this study selected healthy rats,gavage corn oil or corn oil and phospholipid compound,and monitored the changes of body weight,water consumption,blood sugar and serum phospholipid content during blood collection at 0,1,2,4,6,12,and 24 hours.Results showed that compared with the control group,egg yolk phospholipid and soybean phospholipid with different fatty acid composition can inhibit the weight loss caused by blood collection within 0-12 hours (P<0.05).There is a tendency to increase the amount of water consumed,and there is no significant difference.After replenishing phospholipids,the blood glucose level first increased and then decreased,egg yolk phospholipids had the best effect on smoothing blood glucose.Egg yolk phospholipids and soybean phospholipids can significantly improve the disorder of lipid metabolism and the loss of phospholipids caused by blood collection.In conclusion,both egg yolk phospholipid and soybean phospholipid can improve stress hyperglycemia after hemorrhage,and phospholipid has the potential to treat traumatic blood loss.
  • GUO Honghui, CHEN Hui, YU Yun, FANG Hua, CHEN Sijin, HONG Zhuan
    Food and Fermentation Industries. 2020, 46(23): 41-46. https://doi.org/10.13995/j.cnki.11-1802/ts.024631
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    In this paper,the main components,molecular weight distribution and amino acid composition of collagen peptide chelated zinc from puffer skin were analyzed and compared.Its toxicological safety was preliminarily evaluated by acute toxicity test.The results showed that the protein content of collagen peptide chelated zinc from pufferfish skin was (82.04±0.68)%,and the zinc content was (12.86±1.24)%.The molecular weight of collagen peptides from puffer skin was mainly concentrated around 600 Da.Amino acid analysis indicated collagen peptide chelated zinc from puffer skin contained rich glycine,hydroxyproline,aspartic acid and glutamate.The MALDI-TOF mass spectrometry showed the generation of the peptide-zinc chelate.Through the acute toxicity test,the LD50 of collagen peptide chelated zinc from puffer skin was 6.847 9 g/kg,and the 95% confidence limit was 6.122 4~7.676 5 g/kg.According to the acute toxicity dose grading standard,the collagen peptide chelated zinc from puffer skin was actually non-toxic.
  • GUO Zhuang, WU Puying, ZHAO Nan, ZHANG Zhendong, HOU Qiangchuan
    Food and Fermentation Industries. 2020, 46(23): 47-51. https://doi.org/10.13995/j.cnki.11-1802/ts.024255
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    In the study,the quality of Paocai brine was evaluated by electronic tongue and electronic nose,then the microbial community of Paocai was analyzed by MiSeq high throughput sequencing,and the relationship between the quality of Paocai brine and bacterial community was studied.It was found that the difference in sourness was the greatest among all taste indexes,and there were great differences in the relative intensity of aromatics,nitrogen oxides and ethanol.The bacteria in Paocai brine were mainlyPediococcus (55.53%) andLactobacillus (29.83%),which belong to Firmicutes.Meanwhile,the bacterial profiles of different samples were different at genus level.Procrustes analysis showed that the structure of the bacterial community and quality of Paocai brine were significantly correlated (P=0.001),while Pearson correlation analysis showed thatLactobacillus had a significant positive correlation with the response values of 3 sensors,which are sensitive to aromatic compounds (P<0.05).Thus,it can be seen that Paocai brine quality was affected by the bacterial community,andLactobacillus had significant influence on the quality of Paocai.
  • LI Hao, LIANG Qi, YANG Huilin, WEN He, WANG Xiaolan
    Food and Fermentation Industries. 2020, 46(23): 52-59. https://doi.org/10.13995/j.cnki.11-1802/ts.024799
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    In order to analyze the differences on bacterial succession ofAspergillus-type Douchi during rapid and traditional fermentation,direct culture and high-throughput sequencing methods were applied in sample analysis during fermentation.The results indicated that compared with the traditional technology of salt fermentation,the temperature of the rapid technology without mixing salt was over 10?C higher for each period,and the pH values of both technologies were decreasing steadily.The total amount of cultivable microbes in both processes continued to decline with time,and the bacteria were absolutely dominant,which mainly containedBacillus,Staphylococcus andLactobacillus.The dominant phylum in both processes was Firmicutes,and the dominant genera for rapid fermentation wereBacillus,Lysinibacillus andPaenibacillus,whileStaphylococcus,Lactobacillus,Corynebacterium andWeissella for traditional fermentation,the specific genera of both technologies accounted for less than 5%.The fluctuation of bacterial diversities was significant under both technologies,while the abundance of bacteria was lower for rapid processes,and the proportion of genes annotated as relating to proliferation pathways were also significantly lower (P<0.05).The results indicated that the bacteria may maintained low activity state under rapid process,which was not conducive to the flavor of Douchi.Therefore,it is necessary to further optimize the rapid process in subsequent production.
  • LI Wei, LUO Shenbin, QIU Zerui, LAI Denglei, WANG Hongwei, SUO Huayi
    Food and Fermentation Industries. 2020, 46(23): 60-67. https://doi.org/10.13995/j.cnki.11-1802/ts.024566
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    Using high-throughput sequencing method,the community structure and dynamic succession of the microorganisms inMucor-type Yongchuan Douchi during koji making stage and post fermentation stage were analyzed.The results showed that the bacterial diversity in whole process of fermentation was higher than that of fungi,and the diversity of microorganism community during koji making stage was more abundant than that of the post fermentation stage,with more significant community succession.In total,there were 17 fungal and 25 bacterial genera with relative abundance more than 5%.During koji making stage,the dominant fungi consisted of MucorandPenicillium, and the dominant bacteria wereLeuconostoc and Weissella.During the post fermentation stage, Aspergillus, Penicillium, Bacillus, Delftia and Rhizobiumhad relatively higher abundance,while a few fungi,which are mainlyMucor,played a key role with relatively lower abundance.Bacillus,Staphylococcus andCorynebacteriumwere crucial to the whole fermentation process.The result lays a foundation for the regulation and quality control of theMucor-type Douchi and the development of composite fermentation agent.
  • ZHANG Caiwen, LI Jinxia, CHEN Yiwen, DU Haibo, LIU Jiaqi, XIN Di, SHI Liang, CUI Shenhui, YAO Su
    Food and Fermentation Industries. 2020, 46(23): 68-73. https://doi.org/10.13995/j.cnki.11-1802/ts.025322
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    Taxonomic identification and virulence gene verification of 7Escherichia coli strains were carried out,and the feasibility of using them as the standard strains of GB 4789.6—2016 “Microbiological examination of food—Examination of diarrheogenicE.coli”was evaluated.Seven strains ofE.coli were identified by polyphase-taxonomy identification method;according to the methods of GB 4789.6—2016 standard,virulence gene verification of 7 strains of E.coli were carried out.Results showed that the strain CICC 10003 carried geneuidA;CICC 10667 carried genesuidA, sthand lt;CICC 10670 and CICC 24187 carried genesuidA,escV,stx1 andstx2;CICC 24186 carried genesuidA,pic,astA andaggR;CICC 24188 carried genesuidA andinvE;CICC 24189 carried genesuidA,bfpB andescV.The strains CICC 10667,CICC 24186,CICC 24188 and CICC 24189 were identified as enterotoxigenicE.coli (ETEC),enteroaggregativeE.coli (EAEC),enterotoxigenicE.coli (EIEC) and enteropathogenicE.coli (EPEC),respectively;CICC 10670 and CICC 24187 belonged to enterohemorrhagicE.coli(EHEC);CICC 10003 belonged to non-diarrheogenicE.coli.The performance of the 7 strains met the requirements of the corresponding types ofE.coliin the GB 4789.6—2016 standard,and they can be used as standard strains for the examination of diarrheogenicE.coli.
  • LI Song, XU Zhaoxu, LIN Chenying, WEI Shenhua, LI Yanbin
    Food and Fermentation Industries. 2020, 46(23): 74-80. https://doi.org/10.13995/j.cnki.11-1802/ts.024654
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    Eight filamentous fungi with feruloyl esterase ability were screened,and one with the highest yield of feruloyl esterase was identified asAspergillus terreus through sequencing and alignment of 5.8S-ITS.Single factor experiment and response surface methodology were adopted to optimize the liquid fermentation medium ofA.terreus and the optimum medium formula (g/L) was glucose 30,brewer's spent grains 24.5,bean dregs 81,FeSO4·7H2O 0.01,KCl 0.5,MgSO4 7H2O 0.5,K2HPO4 1,with pH 5.0.Under the optimized medium,the highest yield of feruloyl esterase in shaking flask fermentation was 205 U/L,which was about 3 times of the initial yield.The isolated feruloyl esterase producing strain and the optimized liquid fermentation conditions obtained in this study lay a foundation for the breeding of high-yield feruloyl esterase producing strain with an aim for further industrial application.
  • WANG Baoshi, TAN Fengling, LI Guangyao, Chen Yanyan, MA Shihang, ZHANG Mingxia, MENG Li, QIU Liyou
    Food and Fermentation Industries. 2020, 46(23): 81-85. https://doi.org/10.13995/j.cnki.11-1802/ts.024690
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    Starch is a natural macromolecule polymer,which is the main energy material for human and one of the most important industrial raw materials.However,there are still bottlenecks for its traditional enzymatic hydrolysis at high temperature,especially the pretreatment and enzymatic hydrolysis of starch-sugar platform.As a non-thermal processing technology,ultrasound has the advantages of energy saving and high efficiency in mass transfer,hence has become a hotspot in starch modification.In this paper,the application of ultrasound in starch modification,multi-scale structure change and its mode of action are reviewed.For environment friendly and intelligent manufacturing of starch,it is of great significance to explore the mode of action in ultrasound assisted enzymatic hydrolysis reaction,establish novel enzymatic hydrolysis reaction system of starch,and develop novel equipment for ultrasound assisted enzymatic hydrolysis at industrial scale.
  • QIANG Mengjie, LU Xiaoxiang
    Food and Fermentation Industries. 2020, 46(23): 86-91. https://doi.org/10.13995/j.cnki.11-1802/ts.024948
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    The essential oil of argyi leaves has antibacterial,anti-inflammatory and antioxidant effects,but its disadvantages such as poor solubility,easy decomposition and pungent smell limit its application in the field of food.In this study,the phase behavior,microstructure and free radical scavenging rate of the essential oil of argyi leaves microemulsion were studied by phase transformation titration.The emulsification area of the quasi-ternary system composed of Tween 80/anhydrous ethanol/Argyi leaves essential oil/deionized water reached 53.87%.The viscosity change of the microemulsion measured by rheometer could be divided into oil-in-water (W/O) type,bi-continuous phase (B.C) and oil-in-water type microemulsion (O/W).The water content was 90% of,Zeta (-2.18±0.77) mV,particle size (39.91±1.55) nm and PDI (0.28±0.068).The surface tension of the emulsion ranged from 20-30 mN/m and 35-50 mN/m at 20 ℃ and 30 ℃.The microemulsion system with 90% water content was photographed by transmission electron microscopy,and the surface of the particles showed concave-convex spherical or quasi-spherical distribution.At normal temperature and low speed,centrifugation is more beneficial to the storage of microemulsion.The free radical scavenging rate of 1,1-diphenyl2-nitrophenylhydrazine was increased with the increase of water content.
  • LIN Liu, TAO Ningping
    Food and Fermentation Industries. 2020, 46(23): 92-98. https://doi.org/10.13995/j.cnki.11-1802/ts.024932
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    The digestive behavior of micro/nano particles (MNPs) in big eye tuna head soup after optimizing the key exercise parameters of thein vitro biomimetic digestive system was studied.The gastric emptying curve was fitted to the datain vivoby adjusting the gastric tilt angle and the movement parameters of the pylorus,the pH in the stomach was adjusted by adding HCl during the digestion of the stomach to make it close to the real digestive environment of the stomach in the human body.An optical microscope was used to study the microstructure changes of MNPs in the soup.The results show when the gastric tilt angle movement parameter were 0°/min at 0-1 min,3°/min at 1-4 min,0.1°/min at 4-31 min,1.0°/min at 31-61 min,0.4°/min 61-91 min and 0.1°/min at 91-121 min.At 0-6 min,the pylorus was closed.At 6-121 min,the pylorus was opened when the gastric was squeezed 3 times.Backup pump was used to add 1 mol/L HCl with 0.3 mL/min at 1-21 min.The digestion conditions were optimal and the digestive properties of fish head soup were similar to the relatedin vivo researches.
  • GUO Quanyou, LI Song, LI Baoguo, YANG Xu
    Food and Fermentation Industries. 2020, 46(23): 99-107. https://doi.org/10.13995/j.cnki.11-1802/ts.024357
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    To investigate the effect of frozen storage at-18 ℃ on the body color and muscle quality of culturedPseudosciaena crocea,the fresh yellow croaker (F0) was used as a control to determine the difference in body surface color,total carotenoid content,pigment cell size and difference analysis of muscle quality characteristics.The results showed that thea* andb* of lips and muscles were significantly reduced during freezing,and the decrease ofL* anda* in the abdomen and back from F0 to F4 (frozen for 4 months) was relatively smooth,and the decline was significant at F6 (frozen for 6 months);the total pigment content in the abdomen gradually decreased,while the total carotenoid content in the back decreased relatively slowly;with the extension of the freezing time,the black and yellow pigment cells in the fin and scale parts are divergent,and the rate of change in the size of the pigment cells gradually increases,and the skin part shrinks;during frozen storage,the pH and conductivity values ofP.crocea first decreased and then increased,and significantly increased from F4 to F6;the texture characteristics of fish meat deteriorated,except for cohesion,the texture characteristics gradually decreased;juice loss and cooking loss increased significantly during frozen storage,muscle peroxide value and TBA value gradually increased,and increased significantly from F4 to F6,the degree of fat oxidation increased.The results showed that the body color and muscle quality characteristics of the culturedP.crocea frozen at -18 ℃ for six months had gradually declined.In addition to water:2020-06-08retention,the quality of the F4 to F6 stages declined rapidly,which can be used to further explore the mechanism of the body color change provide theoretical reference for quality improvement in the process of freezing storage.
  • CAO Qinglong, LEI Qiao, WU Hao, GAO Wenjing
    Food and Fermentation Industries. 2020, 46(23): 108-115. https://doi.org/10.13995/j.cnki.11-1802/ts.024855
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    To explore the preparation conditions of Pullulan (PUL) aerogels,so as to provide basis for the application of PUL aerogels.PUL aerogels were prepared by sol-gel process and vacuum freeze drying process.The porosity,mechanical strength,microstructure and thermal mechanical properties of the products were analyzed by adjusting the technological parameters such as precursor concentration (0.02-0.1 g/mL),pH value (5.0-9.0) and drying time (1-3 d).The porosity of PUL aerogels increased with the increase of precursor concentration,and the relationship between them wasy=99.11-1.21x.In addition,the porosity increases with the increase of pH and drying time.The compressive strength increased with the increase of precursor concentration (y=-4.19+1.56x),decreased slightly with the increase of pH and decreased with the extension of drying time.The hygroscopicity decreased first and then increased with the increase of precursor concentration,increased with the extension of drying time,but did not change significantly with pH.The loading capacity of Australian tea essential oil decreased with the increase of precursor concentration,and increased with the increase of pH.The best drying condition was 2 days.The aerogel products with good porosity,mechanical strength,low moisture absorption rate and good tea essential oil loading capacity can be obtained by using the preparation conditions of precursor concentration of 0.06 g/mL,pH 8.0 and drying time for 2 days,which meet the requirements of production and application in the future.
  • DUAN Songqi, TANG Tingting, LIU Yixi, LI Cheng, LIU Yuntao
    Food and Fermentation Industries. 2020, 46(23): 116-122. https://doi.org/10.13995/j.cnki.11-1802/ts.024784
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    As a new processing technology,3D printing is able to realize the personalized customization of food appearance and formula,and further improve the consumer's experience of functional food.Based on this,this paper developed a printableAloe vera gel-potato starch gel matrix and loaded different types and additions of food colloids into the gel matrix.The effects of κ-carrageenan gum,gum arabic (GA),sodium alginate (SA),pullulan (PUL),polydextrose (PD),β-cyclodextrin (β-CD) on rheological properties,texture characteristics and microstructure were explored respectively.The printing effect and deviation of different samples were investigated,and the stability of printed samples was also evaluated.The results showed that the 3D printed product with 1.3% κC has the highest accuracy,the most compact microstructure,the best chewing performance.
  • LI Luqian, YAN Qige, HA Yujie, CHEN Lianhong, LI Jian, QIN Fei, DU Rongsheng, WANG Linlin
    Food and Fermentation Industries. 2020, 46(23): 123-130. https://doi.org/10.13995/j.cnki.11-1802/ts.024579
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    The frozenLongissimus Dorsi of yak meat were as the research objects in order to determine and analyze the changes of the food quality,nutritional quality,sensory quality and muscle histological characteristics after different thawing treatments,and to obtain the best thawing method.The results showed that hydrostatic thawing was more beneficial to maintain the pH and water holding capacity than other methods.Meanwhile,hydrostatic thawing and refrigerated thawing were more conducive to maintaining the color stability.Microwave defrosting and refrigeration defrosting could promote the ripening and tenderizing of yak meat.Frozen thawing had the least effect on the sensory quality of yak meat.At the same time,air thawing,refrigerated thawing and microwave thawing had large muscle fiber gap and relatively heavy damage degree of muscle fiber,and still water thawing damage is small.From the above,refrigeration and microwave thawing had a positive effect on maintaining the quality of thawed yak meat on the whole,followed by hydrostatic thawing.The results of this study can provide data support and theoretical basis for the production and processing of thawed yak meat.
  • HU Xiao, XIE Yong, LIU Lin, LIAO Chao, LIU Yue, ZHENG Wanqin, LIU Xiong
    Food and Fermentation Industries. 2020, 46(23): 131-138. https://doi.org/10.13995/j.cnki.11-1802/ts.024818
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    To improve the eating quality of traditional pork meatballs,whole purple sweet potato powder (WPSPP) was used to replace some cassava starch to prepare pork meatballs.Texture analysis,volatile flavor analysis and fuzzy mathematics were used to evaluate the eating quality of the pork meatballs.Meanwhile,the basic components,cooking loss,total juice yield and color of pork meatballs with different WPSPP addition (0,5%,10%,15%) were determined in the current study.The results showed that the types and total amount of characteristic flavor substances,such as alcohol,aldehyde,ketone,ester,in the pork meatballs increased when increasing the WPSPP content.Meanwhile,the hardness,cohesiveness,chewiness and brightness of the pork meatballs gradually reduced,while the spring and cooking loss had no significant change.The total juice yield increased and then decreased with the increasing of WPSPP addition.Notably,the highest sensory score was obtained when the WPSPP content was 5%,and the anthocyanin and total dietary fiber content of the pork meatballs increased by 333% and 40%,respectively.Moreover,more softer in the texture and more attractive in color were observed compared to other addtions of WPSPP.However,the texture and taste of the pork meatballs became worse when the additive amount was 15%.The results indicated that adding WPSPP could not only keep the original eating quality,but also balance the nutritional components and enrich the flavor composition of the traditional pork meatballs.
  • SHU Nan, FU Fuhua, LI Tao, ZHANG Qun, LI Qili
    Food and Fermentation Industries. 2020, 46(23): 139-146. https://doi.org/10.13995/j.cnki.11-1802/ts.024751
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    In order to study juice processing quality characteristics of navel orange varieties in different producing areas of Hunan province,17 indexes including appearance,physicochemical and nutrition of 20 varieties from 10 main navel orange producing areas were determined and their significance was analyzed.Principal component analysis(PCA) was used for analysis and comprehensive evaluation.The results showed that there were significant differences in navel orange quality from different producing areas of Hunan province (P<0.05).Navel orange seeds,titratable acid and total phenol content were significantly different,and the coefficient of variation were 382.43%,37.08% and 31.79%,respectively.While the fruit longitudinal diameter,transverse diameter and juice yield were slightly different,and the coefficient of variation were 9.22%,7.62% and 4.65%,respectively.The results of factor analysis showed that the first five principal component factors including size,function,processing,nutrition and color were extracted with eigenvalue over 1 and a cumulative contribution rate of 82.514%.Based on the analysis of five factors,Newhall navel orange Ⅴ,Newhall navel orange Ⅹ,Newhall ⅩⅠ,and ‘Yizhang' navel orange showed the best suitability for juice processing.The results of this study could provide references for selecting proper navel orange varieties for juice processing in producing areas of Hunan province.
  • CHEN Hua, WANG Jianjun, WANG Jianwu, AI Yinting
    Food and Fermentation Industries. 2020, 46(23): 147-155. https://doi.org/10.13995/j.cnki.11-1802/ts.024965
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    The purpose of this study is to explore the antagonistic yeast compound technology for postharvest storage ofMalus micromalus fruit,Botrytis cinerea was inoculated into the fruit ofM.micromalus,andCryptococcus laurentii was used as the antagonistic yeast to determine the optimal concentration of yeast for biocontrol.Then,the effect of antagonistic yeast combined with water-soluble chitosan (WSC) on the storage quality ofM. micromalus fruit at room temperature was studied.The results of the optimal concentration biocontrol test of antagonistic yeast showed that the yeast solution of 105-108 CFU/mL could significantly reduce the incidence and diameter of the disease in the storage process of the fruit (P <0.05),and the bacteriostatic effect was best under the 107 CFU/mL of yeast solution.The storage quality ofM.micromalus fruit showed that the lowest content of malonaldehyde and maximum activity of antioxidant enzyme in the 7.50 g/L WSC composite treatment group,the weight loss rate,decay rate,soluble solids and VC in the 10.00 g/L WSC composite treatment group were better than other groups.From the 9th day of storage,the indexes of 7.50-10.00 g/L WSC treatment group were significantly(P<0.05)better than yeast treatment group,but there was no significant difference between 7.50 g/L and 10.00 g/L WSC treatment group.In order to reduce cost,postharvest storage ofM.micromalus fruit should be treated with 107 CFU/mLC.laurentii combined with 7.50 g/L WSC.
  • ZHANG Xiaojuan, LIU Guishan, YU Jiangyong, LI Xiaorui, ZHANG Haonan
    Food and Fermentation Industries. 2020, 46(23): 156-161. https://doi.org/10.13995/j.cnki.11-1802/ts.024727
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    In order to study the effect of vacuum precooling on storage property of post-harvest soybeans,this experiment used the fresh soybean as test material to find the optimum vacuum precooling parameters and compare the changes of quality indexes during storage.The results showed that the optimal precooling parameters were as follows:precooling final temperature was 5 ℃,water replenishment was 3%,treatment capacity was 2.5 kg.At that moment,the weight loss rate was 2.8%,the precooling time was 780 s,and the storage quality was good after vacuum precooling.Compared with the cold storage precooling and the control group,vacuum precooling could effectively maintain the nutritional quality and delay the change of respiration rate and hardness during storage.Meanwhile,it could inhibit the increase of weight loss rate and malondialdehyde contents of soybean.Therefore,vacuum precooling could effectively reduce the nutritional loss,inhibit deterioration of soybeans and extend shelf life.
  • LI Yuehong, RAN Maoqian, XU Menghuai, CHEN Lu, YOU Yuanding, ZHAO Yang, JIAO Yanchao
    Food and Fermentation Industries. 2020, 46(23): 162-168. https://doi.org/10.13995/j.cnki.11-1802/ts.024429
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    In order to scientifically evaluate the fruit quality of kiwifruit from Liupanshui city in Guizhou and establish the quality comprehensive evaluation model,different varieties of kiwifruit from Liupanshui were used as experimental materials,the appearance quality and nutrient component of different kinds of kiwifruit were measured and compared by Chinese standard method and agricultural standard method.The quality indexes were analyzed by cluster analysis and the principal component analysis was applied to evaluate the fruit quality of kiwifruit.The results showed that the quality indexes of different varieties of kiwifruit were different and correlated.The difference of Fe was largest and the difference of longitudinal diameter was smallest.The principal component analysis showed that three principal components represented the 14 quality indexes of different varieties of kiwifruit,and their cumulative variance contribution rate was up to 95.890%,making 95.890% cumulative contribution to the total information.According to the cluster analysis,the 14 quality indexes can be divided into four categories.Four quality indicators including sugar-acid ratio,TSS,vitamin C and Fe were screened out by using correlation analysis and principal component analysis as the core indicators to comprehensively evaluate the fruit quality of kiwifruit.The comprehensive evaluation results showed that Hongyang and Donghong were of good quality and suitable for cultivation as fresh food varieties,while Cuixiang and Xuxiang were of poor quality and suitable for cultivation as processing varieties.
  • SHI Jiangchun, XIAO Yuxue, FENG Guiqiang, CAI Tian, CHEN Kewei
    Food and Fermentation Industries. 2020, 46(23): 169-177. https://doi.org/10.13995/j.cnki.11-1802/ts.024609
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    In order to explore the relationship between the appearance color and photosynthetic pigment content of different dried laver and roasted laver (Pyropia yezoensis),the correlation between color index and photosynthetic pigment content of six dried laver and five roasted laver was analyzed.The results showed that the photosynthetic pigment in dried laver was significantly different (P<0.05) from roasted laver.The most abundant photosynthetic pigments in dried laver and roasted laver were phycocyanin,phycoerythrin,allophycocyanin and chlorophyll a.The photosynthetic pigment content of dried laver and roasted laver had different degrees of correlation (|r|=0.037-1.000,|r|=0.041-0.994);the photosynthetic pigment content was also correlated with the color index although not so obvious (|r|=0.033-0.994,|r|=0.034-0.914).Through a multi-parameter prediction model for photosynthetic pigment content established by stepwise regression analysis,it was found that the correlation coefficients between the predicted and actual values of photosynthetic pigment content were improved to some degrees,and it showed phycocyanin content was very accurate for the prediction of total photosynthetic pigment content in laver (P<0.01).This study will provide a reference basis for the quality evaluation of commercially produced laver products.
  • LIN Chunxiu, LIN Yizi, SHEN Shaodan, TENG Qiaoqiao, CAO Yong, CHEN Yunjiao
    Food and Fermentation Industries. 2020, 46(23): 178-183. https://doi.org/10.13995/j.cnki.11-1802/ts.024624
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    A compoundMomordica charantia andCyclocarya paliurus tea (compound tea) was found to not only inhibit the accumulation of reactive oxygen species and age pigments and improve neuroprotection,but also alleviate fat accumulation in previous study.The model of Caenorhabditis elegans was used to further study its antioxidative stress activity,which provided a theoretical basis for further promotion and application.First,it provided the protection toC.elegans exposed to H2O2-and paraquat-induced oxidative stress,especially in the mutant TK22 sensitive to oxidative stress.Further exploration of its antioxidant properties revealed that the antioxidant defense system ofC.elegans was significantly activated by compound tea.In addition,the expression of antioxidant enzyme genes (sod-3,sod-5,ctl-1,andctl-2) and stress resistance genes (hsp-16.1 andhsp-16.2) were also significantly increased.Finally,it was found that y did not damage the reproduction and development ofC.elegans,and even alleviated the decline of mobility.Overall,these results provided promising data for compound tea as a dietary adjuvant in oxidative stress management,and added value toMomordica charantia andCyclocarya paliurus by seeking new and innovative applications.
  • YAN Junling, FAN Yang, QIN Chuan, OU Xue, CHEN Shujuan, AO Xiaolin
    Food and Fermentation Industries. 2020, 46(23): 184-192. https://doi.org/10.13995/j.cnki.11-1802/ts.024733
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    The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from the shoots ofP.amarus,and to determine its anti-inflammatory activity in vitro.Before the dynamic adsorption and desorption tests,the resin screening,adsorption kinetics,adsorption isotherm and thermodynamics were studied successively.The screening showed that AB-8 resin is the most suitable resin for purifying the total flavonoids of the shoots ofP.amarus.The static experimental data correlated well with the pseudo-second-order kinetics model and Langmuir isotherm model.The adsorption process is spontaneous and exothermic.The optimum enrichment conditions were as follows:for adsorption the total flavonoids concentration,flow rate and volume of sample were 1.55 mg/mL,1 BV/h and 4.2 BV.Respectively,and for desorption the flavonoids-loaded AB-8 resin column was desorbed by 8 BV of 50% ethanol at a rate of 4 BV/h,the recovery yield of total flavonoids was 75.03%,total flavonoids content increased from 2.46% to 15.72%.The total flavonoids of the purified could better inhibit the production of NO.When the total flavonoids concentration reached 1.04 mg/mL,the inhibition rate reached 97.80%(the concentration of NO was 5.60 mol/L),while the concentration of NO in the crude extracts was 13.81 mol/L,inhibition rate was only 73.22%.In summary,the purification method of total flavonoids of the shoots ofP.amarus established in this experiment is effective and feasible.
  • YU Yao, LI Yansong, LU Shiying, YANG Yong, HU Pan, REN Honglin, LIU Zengshan, ZHOU Yu
    Food and Fermentation Industries. 2020, 46(23): 193-197. https://doi.org/10.13995/j.cnki.11-1802/ts.024301
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    Based on a monoclonal antibody(McAb),an indirect competitive enzyme linked immunosorbent assay (ic-ELISA) was developed for detection of fumonisin B1 (FB1)and B2(FB2).Experimental parameters,including amount of the coating antigen and McAb,coating conditions of the antigen,dilution rate of horseradish labeled secondary antibody (HRP-IgG) and time of chromogenic reaction were optimized.The linear detection range of the ic-ELISA was 1.41-54.2 ng/mL.The detection limit was 24.5 μg/kg,and the recovery rate of FB1 and FB2 from maize samples was from 89.36% to 108.54%.The cross-reaction rate of this ic-ELISA with FB1 and FB2 were 100% and 129.88% respectively,and lower than 10% with other mycotoxins.The ic-ELISA can be used for detection of the total amount of FB1 and FB2 in corn samples.
  • HU Yuanqing, SHEN Zichen, LI Fengxia, LYU Linxue, ZHOU Zanhu
    Food and Fermentation Industries. 2020, 46(23): 198-206. https://doi.org/10.13995/j.cnki.11-1802/ts.024574
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    A highly specific loop-mediated isothermal amplification (LAMP) assay targeting theblaCARB-17 gene was developed for rapid and sensitive detection ofVibrio parahaemolyticus in aquatic products.The assay was optimized and conducted at 63 ℃ for 60 min usingBacillus stearothermophilus(Bst) DNA polymerase.Amplification was analyzed via 20 g/L agarose gel electrophoresis followed by SYBR Green Ⅰ staining.The specificity was determined by detectingV.parahaemolyticus ATCC 17802 and other 5 foodborne pathogens.The sensitivity was evaluated by detecting diluted genome DNA samples.The reliability was proved in both simulation experiment using experimentally contaminated shrimp samples and detection of aquatic samples from fish markets.The optimum detection condition was:2.4 mmol/L Mg2+,0.96 mmol/L dNTPs,4.8 UBst DNA polymerase,the ratio of inner and outer primer was 8:1,and react at 65 ℃ for 60 min.The result of specificity showed thatV.parahaemolyticus ATCC 17802 was positive,and other 5 control strains were negative.The detection limit of LAMP assay was 3.64×102 ng/μL,and the detection limit in the simulation experiment was 10 CFU/mL.The LAMP assay showed 100% consistency with conventional PCR for detecting practical samples.The LAMP assay established in this experiment is convenient,sensitive and specific,and is suitable for rapid in-field detection ofV.parahaemolyticus.
  • SHI Yi, WU Songwen, LI Wenjuan, YUAN Lu, LI Yan, ZHANG Haofan, MA Guozhu, LIU Dongli
    Food and Fermentation Industries. 2020, 46(23): 207-212. https://doi.org/10.13995/j.cnki.11-1802/ts.024386
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    This study aims to analyze the microorganism changing on eggshells from 3 different areas by quantitative test.The eggs were stored at 25°C for 56 days.Bacterial samples were collected from eggshells on day 0,14,28,42 and 56.The standard curve lines were made for 4 dominant bacteria (Staphylococcus,Lactobacillus,Streptomyces andEnterococcus) using pMD18-T as control referee.The results of quantitative polymerase chain reaction (qPCR) were analyzed statistically by using a one-way analysis of variance method.The results showed that the amount ofStaphylococcus andLactobacillus increased in the first 14 days of the storage time but declined afterwards.There was a continuous increase ofStreptomyces.The number ofEnterococcus decreased from day 28.The amount ofStaphylococcus andEnterococcus was significantly different among groups (P<0.05).There was no significant difference among areas regardingLactobacillusandStreptomyces.These results revealed that the contamination on eggshells could be evaluated by real-time qPCR accurately.The current study may offer new insight into study on the shelf-life of fresh eggs.
  • TAN Ailing, WANG Xiaosi, CHU Zhenyuan, ZHAO Yong
    Food and Fermentation Industries. 2020, 46(23): 213-219. https://doi.org/10.13995/j.cnki.11-1802/ts.024847
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    In order to explore the feasibility of spectral fusion combined with deep learning for quantitative detection of maize components,one-dimensional convolution neural network (1D-CNN) models were constructed for the original,first-order derivative,second-order derivative spectra and the first three types of serial fusion spectra of 80 maize samples,then quantitative regression models of four components of moisture,oil,protein and starch in maize samples were built.The results showed that the performance of the four component models of the 1D-CNN based on serial fusion spectra were all superior to the other three models based on a single spectrum.Compared with the traditional partial least squares and support vector machine regression,the performance of the quantitative modes established by this method is optimal.For the test set,the coefficients of determination and root mean square errors of the four component models were 0.956 and 0.211,0.972 and 0.118,0.982 and 0.239,0.949 and 0.428,respectively.The experimental results showed that the method of serial spectrum fusion combined with convolutional neural network can fully mine the information contained in the spectrum,thus to enhance the model prediction ability,which provides a new idea for the quantitative analysis of near infrared spectroscopy.
  • SUN Yulin, ZHAO Juan, LUO Qinqin, LI Xuyan, ZENG Lin, LI Rui, PAN Qiting, WANG Liyun, CHEN Daohai
    Food and Fermentation Industries. 2020, 46(23): 220-227. https://doi.org/10.13995/j.cnki.11-1802/ts.024793
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    In order to evaluate the nutritional value and provide the basis data for the development of marine functional food,the general nutrients,fatty acids,amino acids,cholesterol,taurine,vitamins and inorganic elements inNotarchus leachii freeri eggs were analyzed.The results showed that the contents of moisture,crude protein,crude fat and ash inNotarchus leachii freeri eggs were 90.73%、2.53%、0.62% and 1.76%,respectively.Twenty-four kinds of fatty acids were detected.The contents of the unsaturated fatty acids were rich,accounting for 57.51% of the total fatty acids content,and the total amount of EPA and DHA accounted for 27.28%.Seventeen amino acids were found inNotarchus leachii freeri eggs.The contents of the essential amino acids (EAA) and delicious aminoacids (DAA) were 7.41 g/100 g and 6.86 g/100 g,respectively,accounting for 34.76% and 32.18% of the total amino acids (TAA).The EAA composition basically met the FAO/WHO criteria.According to nutritional evaluation in amino acid score (AAS) and chemical score (CS),the first limiting amino acid ofNotarchus leachii freeri eggs was methionine.The contents of cholesterol and taurine were 19.8 mg/100 g and 180 mg/100 g,respectively,inNotarchus leachii freeri eggs,and the ratio of taurine to cholesterol (T/C) was 9.09.Their vitamins VD、VE and VB2contents were 47 μg/100 g,42.2 mg/100 g and 0.02 mg/100 g,respectively.The macroelements (Ca,Mg,K and Na) and trace elements (Fe,Zn,Mn and Se,etc.) were abundant inNotarchus leachii freeri eggs.In summary,Notarchus leachii freeri eggs are rich in various nutrient elements with a balanced proportion,and are high-quality seafoods that meet the nutritional needs of human body.
  • Dongxu, HU Qijie, WANG Fengli, WANG Xincai, LI Fen, CHEN Chujian
    Food and Fermentation Industries. 2020, 46(23): 228-232. https://doi.org/10.13995/j.cnki.11-1802/ts.024801
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    A ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of maillard reaction product [5-(5,6-Dihydro-4H-pyridin-3-ylidenemethyl)furan-2-yl]methanol (F3-A) in roasted coffee beans was constructed,and the contents of F3-A in coffee beans roasted to different degrees were detected by using this method.The sample was extracted with 1 mol/L HCl in an ultrasonic bath,purified by MCX solid phase extraction column.The separation of F3-A was performed on a Waters ACQUITY UPLC BEH C18 column (100 mm×2.1 mm,1.7 μm),and determined in the positive MRM modes by MS/MS using external standard method.The method showed a good linearity over the range of 10-2 000 μg/kg withr2=0.999.Recovery rates of F3-A were 85.7%-93.3% at three spiked levels in roasted coffee beans and the relative standard deviations were 2.56%-3.97%.The roasted coffee bean samples results showed that with increasing roasting degree,the formation of F3-A was found and the contents of F3-A first increased and then decreased,light roasting degree showed the maximum F3-A content.The types of coffee bean,the roasting temperature and the roasting time were the major factors in the formation of F3-A during roasting coffee beans,the optimum conditions for the formation of F3-A were Kenya++ coffee beans roasted at 200 ℃ for 10 min,and the content of F3-A was 329.5 μg/kg.
  • WANG Suli, FEI Huaxi, ZHOU Can, YE Chenzhi, JIANG Wenping
    Food and Fermentation Industries. 2020, 46(23): 233-237. https://doi.org/10.13995/j.cnki.11-1802/ts.024502
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    DNA-Cu(II) complex with peroxidase-like activity was formed through coordination reactions of Cu2+ with specific DNA sequence.Because of the peroxidase-like activity,this complex could accelerate the reaction rate of 3,3′,5,5′-tetramethylbenzidine-H2O2 and led to a distinct color change of the solution from colorless to blue.On this basis,a colorimetric method was developed for the detection of Cu2+ in grain while the samples were pretreated by dry ashing method.Good quadric relationship was revealed for the standard curve in the range of 0-1.0 μg/mL with a correlation coefficientR2 of 0.998 and a detection limit of 0.01 μg/mL.The determination of four certified reference samples showed that the deviation was -9.2% to +8.2% and coefficient of variation ranged from 3.93% to 6.32%.Ten positive grain samples were determined by both this colorimetric method and flame atomic absorption spectrophotometry,no significant difference was found in the detection results.This method was simple and accurate,which was suitable for the determination of copper in grain.
  • NING Yawei, MA Mengge, YANG Zheng, HOU Linlin, ZHAO Zhongqing, CHEN Yi, WANG Zhixin, JIA Yingmin
    Food and Fermentation Industries. 2020, 46(23): 238-247. https://doi.org/10.13995/j.cnki.11-1802/ts.025341
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    γ-aminobutyric acid(GABA),a natural four-carbon non-protein amino acids,has gained increasing attention due to the beneficial physiological functions,such as anti-hypertension,anti-depression,anti-anxiety,improving brain function.The natural content of GABA in plants and animals is low,leading to high production cost.Therefore,an efficient production method for GABA needs to be developed.The enrichment for GABA mainly includes plant enrichment and microbial synthesis.The enriched GABA can be used for the development of various food rich in GABA (such as grain products,dairy products,meat products) to meet people's requirement for healthy food.Therefore,the synthesis mechanism,enrichment technology of GABA,and the development status of GABA functional food were briefly introduced,aiming to provide theoretical reference for the enrichment of GABA and the development of functional food.
  • XU Zheng, ZHU Yafeng
    Food and Fermentation Industries. 2020, 46(23): 248-254. https://doi.org/10.13995/j.cnki.11-1802/ts.024471
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    Cellobiose 2-epimerase has attracted growing attentions as an industrial enzyme.It was applied to lactulose bio-production,as well asD-mannose preparation.Lactulose was used for treating constipation and hepatic encephalopathy as a common over-the-counter drug,and showing a high efficiency.The annual global demand for lactulose was nearly 100 000 tons,showing great commercial value.In this review,recent advances in new enzyme characterization,protein engineering and enzyme immobilization for cellobiose 2-epimerase are summarized,which would shed light on researches in this field.
  • YANG Yang, WANG Songtao, XU Zhenghong, SHI Jinsong
    Food and Fermentation Industries. 2020, 46(23): 255-262. https://doi.org/10.13995/j.cnki.11-1802/ts.024607
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    Hemicellulose is one of the most abundant and low-price renewable resources on the earth.As a collection of important biocatalysts,the hemicellulase system possesses high application value in degradation and utilization of hemicellulose,which is worthy studying in details.Based on the close correspondence between hemicellulose and the microbial enzymes involved in its complete breakdown,the molecular structure characteristics of hemicellulose as well as the classification,functional features,and development approaches of microbial hemicellulase were summarized.In view of the application of hemicellulase in the fermentation processes of wine,sake,beer,Baijiu and soy sauce,further development in academic research and industrial application of the microbial hemicellulase system was recommended.
  • ZHENG Ruilong, SUO Jingyi, NIU Chengtuo, ZHENG Feiyun, YI Chonghua, SHENG Diqing, WANG Jinjing, LI Qi, LIU Chunfeng
    Food and Fermentation Industries. 2020, 46(23): 263-268. https://doi.org/10.13995/j.cnki.11-1802/ts.024717
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    Purple (black) rice is a kind of colored rice which is widely cultivated in China,and is rich in active substances such as anthocyanins,flavonoids and phenolic acids.Besides,it has anti-cancer,anti-allergic,anti-obesity properties and can prevent arteriosclerosis and cardiovascular diseases.Due to its characteristics and nutritional components,purple (black) rice has important economic value and market potential in brewing industry.This review summarizes the nutritional value,bioactive functions of purple (black) rice and its applications in brewing industry.Purple (black) rice can be widely applied in brewing industry and improve the added value of wine,which will then promote the diversified development of brewing market.
  • WANG Jingbang, XIE Jing
    Food and Fermentation Industries. 2020, 46(23): 269-278. https://doi.org/10.13995/j.cnki.11-1802/ts.024720
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    Polysaccharide edible membrane(PEM) is characterized by richness,naturalness,biodegradability,which has become a research hotspot in the preservation of aquatic products.This paper briefly introduces the main components,film formation mechanism,functional properties and application in aquatic products of PEM.In addition,it reviews the main performance and modification methods,and proposes that the application of new film formation methods and low-cost and high-efficiency constituent materials is the future research direction.The purpose is to provide a theoretical basis for the application of PEM in the preservation of aquatic products.
  • HAN Xiaomin, LI Fengqin
    Food and Fermentation Industries. 2020, 46(23): 279-285. https://doi.org/10.13995/j.cnki.11-1802/ts.024324
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    Aspergillus nigeris commonly used in food fermentation industry.It was also found in food spoilage.Precise classification and identification ofA.niger isolates are the research hotspot for taxonomists.At present,besides traditional morphological identification,polyphasic classification methods based on features of physiology,extrolites,and molecular biology are exploited in the classification and identification ofA.niger strains.The advantages and disadvantages of each method and its practical application in the identification ofA.niger strains are elaborated in detail.The future development direction about the classification and identification ofA.nigerstrains is put forward.
  • HUANG Xiaoyan, LIU Chengjun, LI Changcheng, LIU Limin, FANG Ting
    Food and Fermentation Industries. 2020, 46(23): 286-292. https://doi.org/10.13995/j.cnki.11-1802/ts.024436
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    Microorganisms are the main factors leading to food spoilage.Because of its low free water content,most microorganisms cannot grow and reproduce in low water activity food.In the past,low water activity food has been considered as no microbial safety hazards.But in recent years,the occurrence of food safety events caused by microorganisms' germination in low water activity food is more and more frequent,especially the presence of some pathogens,which seriously threaten human health.Therefore,extensive attention has been paid to the microbial safety of low water activity food,as well as the microbial control technologies.In this paper,the microorganisms that are easy to survive in low water activity food,and corresponding microbial control technologies,are summarized.
  • WANG Song, GUO Keyu, TANG Lin, YANG Yong, LIU Shuliang
    Food and Fermentation Industries. 2020, 46(23): 293-300. https://doi.org/10.13995/j.cnki.11-1802/ts.024527
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    Application of starter cultures promoted the industrialization of traditional meat products,however,brought to the consumers an impression of monotonous,tasteless and unsafe.Some novel strains of coagulase-negativeStaphylococci (CNS) can improve the flavor,color,safety and health of fermented meat products.In this review,the diversity of CNS in fermented meat products and influencing factors on its dynamic variations are summarized,the technological and safety characteristics of CNS in the production of fermented meat products are discussed,and the screening strategy of novel functional CNS strains are proposed.This review provides reference for the application research of indigenous CNS strains and promote the development of traditional fermented meat industry.
  • LI Anqi, LI Xiaofei, LI Qianjin, YANG Xi, GUO Yurong
    Food and Fermentation Industries. 2020, 46(23): 301-308. https://doi.org/10.13995/j.cnki.11-1802/ts.024536
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    Due to extensive accessibility and abundant types,polysaccharides have been widely used in the food industry as the dispersing agent,stabilizing agent,gelling agent and emulsifying agent.It has been recently reported that,in addition to the conventional gel-set methods,freeze-thaw method is also effective to induce polysaccharide gelation,which enables food polysaccharides to have a greater application prospect.This review firstly introduced the basic principles and formation process of polysaccharide cryogelation,and then listed the examples of polysaccharide cryogelation,and further summarized the main parameters affecting the cryogelation process.Besides,this review also summarized the application of polysaccharides in improving the textures of frozen foods.It is expected that the review can provide a novel approach for promoting the further utilization of polysaccharides in the food industry.
  • ZHENG Xiaoning, LI Jun, MU Jianlou, CHEN Yonghao, HAO Yanbin, QI Jianxun, DONG Ningguang, ZHANG Yunqi
    Food and Fermentation Industries. 2020, 46(23): 309-315. https://doi.org/10.13995/j.cnki.11-1802/ts.024580
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    Plant polyphenols are secondary metabolites with polyphenolic structure in plants.They are widely found in the bark,roots,leaves and fruits of plants and have a variety of biological activities such as antioxidant and anti-inflammatory.The effects of plant polyphenols on the improvement of gastrointestinal function are significant.This paper summarizes the research progress of plant polyphenols in gastrointestinal microflora optimization,mucosal damage recovery,intestinal structure improvement and effects of macromolecules interaction on gastrointestinal function.
  • HU Wenze, LI Miao, GUO Dongxu, SHI Ying, YUE Guoxin, MA Fengming
    Food and Fermentation Industries. 2020, 46(23): 316-322. https://doi.org/10.13995/j.cnki.11-1802/ts.024859
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    Aronia melanocarpa is a new small berry fruit tree,whose fruit contains not only extremely high nutrition,but also contains a lot of polyphenols.Therefore,it is a natural functional food raw material,which has many physiological functions such as antioxidant and so on.However,the storage time and the transportation performance of the freshA.melanocarpa is poor.So further processing of the fresh fruit into wine and other products could effectively avoid these problems.This article summarizes the main active ingredients,physiological functions and products development status ofA.melanocarpa,and also reviews the market prospects ofA.melanocarpa in China,aiming to provide a reference for the further development ofA.melanocarpa.