25 January 2021, Volume 47 Issue 2
    

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  • ZHANG Xiaoxiao, REN Jianxing, LIU Kaiyi, LI Xiao, DONG Jian
    Food and Fermentation Industries. 2021, 47(2): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.024907
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    Saccharomyces cerevisiae is the most common and widely used yeast strain,and it is the most classic strain of ethanol fermentation using saccharide and starch as raw materials.Yeasts are confronted with several inevitable environmental stresses such as hyper-osmotic stress,high temperature,which can negatively affect yeast growth rate and fermentation performance.This will cause some economic losses in related fermentation industry.In order to improve stress tolerance,strain AY12a was used as the starting strain,the URA3 gene was used as a screening marker,and the long primer knockout method was used to realize the gene deletion in this study.Finally,the mutant strain AY12a-tor1Δ was successfully constructed.Tolerance determination of yeast showed that AY12a-tor1Δ had certain high temperature resistance,oxidation environment tolerance and had certain tolerance under other stress conditions.AY12a-tor1Δ and AY12a were participated in high-temperature concentrated fermentation of corn,and the alcohol content,residual sugar,48h cell survival rate,CO2 weight loss and fermentation time after fermentation were determined.The results showed that the ethanol yield of the mutant strain AY12a-tor1Δ increased,the residual sugar content decreased,the cell survival rate did not decrease at 48 h,and the fermentation time was prolonged.
  • HUANG Hao, HA Zude, GU Jingsai, YANG Xinghua, WANG Zhaoji, CHEN Shuang, XU Yan
    Food and Fermentation Industries. 2021, 47(2): 8-14. https://doi.org/10.13995/j.cnki.11-1802/ts.024418
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    CT Using the WL yeast morphological identification medium,a total of 48 strains of typical yeast morphology were isolated and identified from Zangqu (traditional koji for brewing Qingke wine) of different regions.Further,31 Saccharomyces cerevisiae strains,nine Saccharomycopsis fibuliger strains and three Saccharomycopsis malanga strains were identified by molecular identification.The brewing performance of different yeasts was studied by fermentation in Qingke culture medium.It was found that S.cerevisiae was the main alcohols producing microorganism in Qingke wine fermentation.While S.malanga was the main organic acid producing microorganism.Through the analysis of 26 aroma metabolites and comparison of three kinds of yeasts,it was found that S.cerevisiae was the main aroma producing microorganism in Qingke wine,which produced abundant esters,alcohols and organic acids.S.fibuliger had important contribution to trace aromatic substances in Qingke wine,however Saccharomycopsis malanga has weak ability to produce aroma compounds.This paper systematically studied the brewing performance and flavor function of the main yeasts in Zangqu for traditional Qingke wine brewing,and provided a basis for the scientific control of the flavor quality of Qingke wine.
  • CHEN Ke, LIU Lili, HAO Weiming, YANG Xiaopan, LI Yuanyuan
    Food and Fermentation Industries. 2021, 47(2): 15-21. https://doi.org/10.13995/j.cnki.11-1802/ts.022114
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    CT This study aims to investigate the effect of inlet temperature of spray drying on the rheological and structure properties of egg white protein(EWP).EWPs were dried at different drying temperatures (140,160,180 ℃) to determine the rheological and structure properties of the products.The results showed that the storage modulus (G′) was higher than the loss modulus (G″),it could be seen that the elasticity of all samples was better than the viscosity.In the low-frequency range,the order of the G′ value was as follows:140 ℃>180 ℃>160 ℃.However,in high-frequency range,the stability order of G′ was 160 ℃>140 ℃>180 ℃.Moreover,the ratio of bound water (T21) to adsorbed water (T22) peak was 180 ℃>160 ℃>140 ℃,and the order of the proportion of free water (T23) peaks was 140 ℃>160 ℃>180 ℃.The red shift of the amide I band at 180 ℃ to the low wavenumber direction was significantly different (P<0.05).And 180℃ EWP powder has the highest content of β-fold and β-curl which indicating a greater influence on the secondary structure of EWP powders.With the increase of spray drying inlet temperature,the structure of EWP powders was changed from relatively complete to broken,rugged and loosened.The change of these micro morphology might be due to the water removal rate and shear force during spray drying,which led to the breakage of hydrogen bonds between protein molecules and destroy the cross-linking of protein molecules.Results indicated that the spray drying temperature of 160 ℃ promotes the breakage of the molecular chain of EWP,and had a certain degree of aggregation of EWP and decreased in apparent viscosity,which could improve its rheological properties and helped to expand the application of EWP in food,healthcare,biology,medical care and other fields.
  • PENG Songlin, PAN Chenglei, KANG Mengyao, LI Yixuan, ZHAO Ziyue, ZHENG Renbing, SHANG Yongbiao
    Food and Fermentation Industries. 2021, 47(2): 22-29. https://doi.org/10.13995/j.cnki.11-1802/ts.024839
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    In order to improve the stability of color and flavor during the storage of meat products,the properties of melanoidin,one of the main pigments on the surface of meat products,were studied.The extraction process of melanoidin from duck skin was optimized by single factor test and orthogonal test.The antioxidant activity and stability of melanoidin from duck skin were measured.The results showed that the order of the factors affecting the extraction of melanoidin from duck skin was:Ethanol volume fraction >extraction temperature >extraction time >ratio of material to liquid.Moreover,the optimal extraction parameters were 5% ethanol at the ratio of 1∶40 by solid-liquid at 60 ℃ for 4 h.The DPPH radical scavenging ability,reducing power and ·OH scavenging ability of duck skin melanoidin were stronger,and were significantly positively correlated with the concentration.With the prolongation of storage time,the content of melanoidin in the skin of stewed roast duck was increased first and then decreased (P<0.05) and reached the maximum value after 1 month of storage.Illumination time (sunlight),temperature,pH and reducing agent (Na2SO3) all had a certain effect on the stability of melanoidin in the skin of stewed roast duck.So,avoiding sunlight exposure,reducing storage temperature,adjusting pH to neutral or slightly alkaline and adding reductants appropriately can improve the stability of melanoidin.
  • LI Zihe, XIA Yibing, ZHANG Zhong, BI Yang, QIAO Caihong, LI Binshan, WILLIAM OYOM
    Food and Fermentation Industries. 2021, 47(2): 30-37. https://doi.org/10.13995/j.cnki.11-1802/ts.025102
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    The essential oils of four umbellifers (Cuminum cyminum L.,Coriandrum sativum L.,Carum carvi,Anethum graveolens L.) were hydro-distilled and the nanoemulsion of the compound essential oil was prepared.Potato tubers were fogged with the nanoemulsion to test its antigermination effect on potato tubers.To explore the antigermination mechanism,the contents of reactive oxygen species and key antioxidants,and the activities of key antioxidant enzymes of treated tubers were tested.The results showed that the fogging with the nanoemulsion significantly inhibited the germination of potato tubers and also reduced the content of tuber hydrogen peroxide.Besides,it could significantly increase the contents of nicotinamide adenine dinucleotide phosphate (NADPH).Moreover,glutathione and the contents of total flavonoids and total phenols of the tubers were also decreased.Furthermore,the activities of the key antioxidative enzymes in the tuber were significantly elevated by fogging treatment.In conclusion,nanoemulsion of the compound essential oil reduced the content of reactive oxygen species by adjusting the activities of key antioxidant enzymes and antioxidant contents of potato tuber,and thus extended the dormant period of potato tuber and inhibited its germination.
  • WANG Junqi, HUANG Weihong, LI Shuangtong, YUAN Jianjun, CHEN Hongbin, MA Yinglun, ZHANG Qiufang
    Food and Fermentation Industries. 2021, 47(2): 38-44. https://doi.org/10.13995/j.cnki.11-1802/ts.022788
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    In order to explore the characteristics of bacterial and fungal communities and abundance in different periods of Yongchun aged vinegar,representative vinegar samples collected at four important periods,including fermentation (Y1),1-month product (Y2),1-year (Y3) and 10-year storage (Y4),were analyzed by Illumina Miseq high-throughput sequencing of 16S rRNA and 18S rRNA genes.The results showed that Alphaproteobacteria and Bacilli were the dominant bacteria classes in the former three periods (Y1,Y2 and Y3),Acetobacter (>60%) and Lactobacillus (>20%) were the dominant bacteria genera.However,bacterial 16S rRNA gene could not be detected in the vinegar sample of the last period (Y4).For fungal composition,18S rRNA gene could be found in vinegar samples of all four periods.In addition,Saccharomycetes and Eurotiomycetes were dominant at the fungal class level,an unclassified genus of Saccharomycetales (>95%) was the dominant fungal genus in the vinegar of Y1,Y2 and Y3,while an unclassified genus of Trichocomaceae (>50.36%) was the dominant genus in that of Y4,followed by an unclassified genus of Saccharomycetales (28.07%) and Pichia (17.68%).The bacterial composition in Y2 was similar to that of Y3 but was different from that of Y1.On the contrary,there were high similarities of fungal composition among those of Y1,Y2 and Y3,while they were clearly different from that of Y4.Our results provide a scientific evidence for revealing the dynamic changes of bacterial and fungal diversity of Yongchun aged vinegar during the production and storages.
  • LI Xia, CHEN Haiou, HAN Shufang, LU Fengying, ZHOU Yuheng, SHAN Yang, LI Jing
    Food and Fermentation Industries. 2021, 47(2): 45-50. https://doi.org/10.13995/j.cnki.11-1802/ts.024603
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    To explore the potential of carboxymethyl xylan as a prebiotics,we modified by sodium hydroxide chloroacetic acid reaction system was used to carboxymethylate xylan,and the structure was characterized by infrared spectroscopy.Its anti-digestibility was explored by simulating the human digestive environment,and its prebiotic effects on four probiotics (Lactobacillus brevis,Lactobacillus plantarum, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophiles) were studied.The results showed that carboxymethyl xylan had characteristic absorption peak at 1 609 cm-1、1 405 cm-1 and 1 320 cm-1,which indicated that it was successfully modified and the degree of substitution was 0.68.In the digestion experiments of simulated saliva,gastric juice and intestinal fluid,the hydrolysis degree of carboxymethyl xylan was lower than 9%,5% and 6%,respectively,indicating its resistance to digestion in human digestive tract.Moreover,carboxymethyl xylan could promote the growth of four probiotics and the promoting effect increased with its concentration.The optimum concentration of carboxymethyl xylan for the growth and reproduction of probiotics was 3%,reaching the maximum growth within 24-36 hours.Carboxymethyl xylan has the potential to be used as prebiotics.
  • LI Yifei, QIN Xiaoli, KAN Jianquan, LIU Xiong, ZHONG Jinfeng
    Food and Fermentation Industries. 2021, 47(2): 51-56. https://doi.org/10.13995/j.cnki.11-1802/ts.024872
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    In order to further explore the properties of cycloartenyl ferulate,the molecular structure,reactive sites (electrostatic surface potential,the atomic charge,frontier molecular orbital,conceptual density functional activity index),weak intramolecular force,aromaticity and infrared spectrum of cycloartenyl ferulate,were analyzed based on the density functional theory.The results showed that there was a maximum electrostatic potential point (53.580 kcal/mol) near the hydroxyl hydrogen atom (H99),and H99 had a higher positive charge (0.352 a.u.) which indicating that it had strong nucleophilic activity and was prone to nucleophilic reaction.Moreover,there was a minimum electrostatic potential point (-36.400 kcal/mol) near the hydroxy oxygen atom (O4),and O4 has a high negative charge (-0.378 a.u.) and a high f - value (0.078) which indicating that it had a strong electrophilic activity and was prone to electrophilic reaction.Furthermore,the analysis of weak intramolecular force showed that van der Waals force and steric hindrance together maintained the stability of cycloartenyl ferulate.The cycloartenyl ferulate had aromaticity.The theoretical simulated infrared spectrum of cycloartenyl ferulate was similar to the experimental infrared spectrum,suggesting that the density functional theory could be used to research the structure and properties of cycloartenyl ferulate.This article provides a theoretical basis for revealing the relationship between the structure and properties of cycloartenyl ferulate,and also provides a reference basis for the further development of the physiological activity of cycloartenyl ferulate and broadening its application in food.
  • CHEN Zhiyin, LIU Weipeng, WANG Yingxi, ZENG Li, XIANG Guohong, LIU Taoli, GONG Yihui
    Food and Fermentation Industries. 2021, 47(2): 57-62. https://doi.org/10.13995/j.cnki.11-1802/ts.025112
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    The present paper aimed to study the effect of modification methods on the structural and physicochemical properties of insoluble dietary fiber (IDF) from sweet potato residue.The insoluble dietary fiber of sweet potato residue was modified via colloid wet grinding technology,ultrasonic assisted acid method and cellulase enzymatic method.Particle size distribution and morphology of insoluble dietary fiber before and after modification were compared.Results showed that the particle size distribution of IDF from sweet potato residue was significantly decreased by the three modification methods (P<0.01).The water holding capacity and oil absorption capacity were significantly changed (P<0.01),while the nitrite and cholesterol adsorption capacity of IDF were improved by these three methods.In summary,colloid wet grinding technology had the best effects on oil absorption,water holding capacity,nitrite and cholesterol adsorption of IDF from sweet potato residue.Modified IDF from sweet potato residue could be applied as a functional ingredient in diverse food products.
  • WANG Wenxia, YANG Yue, ZHANG Huijun, LIU Bo, XU Tingting
    Food and Fermentation Industries. 2021, 47(2): 63-69. https://doi.org/10.13995/j.cnki.11-1802/ts.024285
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    Rheological properties of potato pectin (PPCH) and the factors,such as pectin concentration,shear rate,temperature,pH value,metalions on rheological properties were investigated.The results showed that the increased pectin concentration resulted in the increase of solution viscosity.However,with the increase of shear rate,the solution viscosity was reduced.Moreover,the shear-thinning became more obvious with the increase of pectin concentration,which could be attributed to non-Newtonian fluid.The rheological behavior of PPCH solution was affected by temperature.At specific shear rates(50 s-1),the viscosity of PPCH solution decreased rapidly with the increase of temperature in range from 10 ℃ to 47 ℃.In addition,the viscosity of PPCH solution fluctuated slowly with the temperature increasing in the range from 47 ℃ to 65 ℃,and the viscosity of PPCH solution decreased slowly as the temperature increase in the range from 65 ℃ to 80 ℃.At specific shear rates(100,500 s-1),the viscosity of PPCH solution decreased slowly with the increase of temperature.In the temperature range from 10 ℃ to 47 ℃,the activation energy of PPCH solution had a greater correlation with the shear rate.In the temperature range from 47 ℃ to 80 ℃,the activation energy had a smaller correlation with the shear rate.The rheological properties of the PPCH solution were influenced greatly by pH value.Besides,the viscosity of pectin solution increased first and then decreased with the increase of pH value.And at pH 4.0,pectin solution had the lowest viscosity and the lowest fluidity.Divalent metalions obviously improved the viscosity of PPCH solution.Compared with Mg2+,Ca2+ could significantly increase PPCH solution viscosity.
  • WU Yun, WANG Chunlin, WANG Lipeng, ZHANG Lala, HU Haobin
    Food and Fermentation Industries. 2021, 47(2): 70-77. https://doi.org/10.13995/j.cnki.11-1802/ts.025714
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    In order to study the adsorption separation and oxidation properties of Lycium ruthenicum Murr.Polyphenols,the static adsorption method was adopted to screen nine different types of macroporous resins.The static adsorption kinetics and thermodynamics of HPD500 macroporous resin on Lycium ruthenicum polyphenols were studied to explore the adsorption mechanism of HPD500 resin.Dynamic purification process and oxidation resistance were also investigated.The results showed that HPD500 macroporous resin showed better adsorption performance than others.The adsorption process was consistent with the pseudo-second-order model and was mainly controlled by the chemical reaction model.Langmuir model could accurately describe the adsorption behavior of HPD500 macroporous resin in the temperature range investigated,and ΔH<0、ΔG<0、ΔS<0 indicating that it was a spontaneous exothermic and entropy-decreasing adsorption process.The optimum purification parameters of dynamic adsorption and desorption were as follow:8 BV of was loaded onto the column at a flow rate of 2.4 BV/h and eluted with 6 BV of 60% ethanol with the same flow rate.Under these conditions,the purity of polyphenols purified increased 2.36 times than control.Oxidation resistance tests showed that purified polyphenols from Lycium ruthenicum espectively.It would promote the development and utilization of Lycium ruthenicum Murr.and lengthen its industrial production chain.
  • LU Yibei, LYU Chunxia, LIAO Huiqi, HU Yuanhui, LEI Yesi, CAO Shaoqian, YANG Hua
    Food and Fermentation Industries. 2021, 47(2): 78-86. https://doi.org/10.13995/j.cnki.11-1802/ts.024125
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    Cultured Pseudosciaena crocea was used as the research object to explore the effect of NaCl on the gel properties of myofibrillar protein added with serine protease.Firstly,the serine protease obtained from the meat of cultured P.crocea was studied and the optimum temperature,optimum pH and the effects of NaCl on serine protease activity were analyzed.Furthermore,the effect of NaCl on the texture,water holding capacity,whiteness,Raman spectrum,fluorescence analysis and microstructure of myofibrillar protein gel containing serine protease were further studied.The results showed that the optimum temperature and pH value of serine protease were 50 ℃ and 7.0 respectively.With the addition of NaCl,the secondary structure of myofibrillar protein gel was changed,and the structure of myofibrillar gel became more compact and stable,but the fluorescence intensity decreased.When the addition of NaCl was at 0%-2%,the gumminess and chewiness of myofibrin gel increased continuously,and the whiteness increased.When the addition of NaCl was 4%,myofibrillar protein gel had the strongest water holding capacity.Therefore,it can be concluded that with the addition of NaCl,the activity of serine protease is inhibited,which weakens the damage of serine protease to myofibrillar protein gel,thus improving the gel properties of surimi.
  • CAI Yawen, ZHANG Xuan, CHEN Ou, DENG Lili, ZENG Kaifang
    Food and Fermentation Industries. 2021, 47(2): 87-94. https://doi.org/10.13995/j.cnki.11-1802/ts.024768
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    Biological control by antagonistic yeast has been considered to be an effective and promising strategy to reduce the postharvest diseases and loss of fruits.To determine the biocontrol effect and phenylpropane mechanism of Pichia galeiformis on the postharvest brown rot of plum (Prunus) caused by Monilinia fructicola during storage,the growth of M.fructicola co-cultured with P.galeiformis and the biocontrol effect under inoculation in the same and different pores and soaking were detected.Also,the changes of the key enzymes and substance content in the phenylpropane pathway after treatment with P.galeiformis were studied.The results showed that P.galeiformis (1×108 cells/mL) had significant biocontrol efficacy against M.fructicola rot of plums:the diameter of M.fructicola colony was significantly reduced after 7 d,the incidence and spot diameter of plum fruit treated with P.galeiformis were significantly lower than those of the control.Furthermore,the activities of key defense-related enzymes including phenylalanine ammonia-lyase,peroxidase,cinnamate 4-hydroxylase,4-coumaric acid coenzyme-A-ligase were potentially induced by this yeast.Besides,the accumulation of metabolites,such as lignin,total phenol and flavonoid,also increased.Consequently,P.galeiformis can control the postharvest brown rot and improve disease resistance of plum.
  • KANG Huifang, QIAO Yongjin, LIU Chenxia, ZHANG Yi, Sun Dapeng, TONG Xiaoyang
    Food and Fermentation Industries. 2021, 47(2): 95-101. https://doi.org/10.13995/j.cnki.11-1802/ts.023594
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    To study the inhibitory effect of ClO2 on Alternaria alternata,and provide basis for reducing the loss caused by A.alternata rot during grape storage,isolated bacteria were treated under different concentrations of ClO2 (1.5,3.0,4.5,6.0,7.5,9.0 μg/L) for 10,20 and 30 minutes respectively,the bacteria without any treatment was used as control group.The inhibition effects of ClO2 on A.alternata were explored by measuring of mycelial growth,spore formation,germ tube elongation and observing of the hypha morphology by scanning electron microscopy.The grapes inoculated with A.alternata were treated with 3.0,6.0 and 9.0 μg/L ClO2 respectively for 30 min and stored in the incubator at 28 ℃.The inhibition effect of ClO2 on A.alternata rot was observed by measuring the diameter of lesions,incidence and related enzyme activities.The results showed that ClO2 at different concentrations could significantly inhibit the mycelial growth,spore germination rate and germ tube elongation of Alternaria spp. In vivo experiments inoculated with A.alternata showed that ClO2 treatment could inhibit the diameter of lesions and the rot rate of fruits,delay the accumulation of malondialdehyde content in fruits and maintain high antioxidant activity.Among them,the grapes treated with 9.0 μg/L ClO2 for 30 minutes had the best fresh-keeping effect.
  • XU Jinzhao, TANG Mengqi, XU Jinghan, TENG Guoxin, XU Xiaoxi
    Food and Fermentation Industries. 2021, 47(2): 102-108. https://doi.org/10.13995/j.cnki.11-1802/ts.024997
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    In this experiment,a high-salt diet model was established in mice.The model mice were given pyroglutamic acid intervention to explore the effect of pyroglutamic acid on intestinal health and intestinal flora in mice with high-salt diet.Pathological analysis and morphological observation were performed on the small intestine of mice,followed by detection of intestinal inflammatory cytokines and albumin leakage in feces.Alpha diversity,beta diversity,and intestinal flora composition at the phylum and genus levels were analyzed by high-throughput sequencing.The results showed that pyroglutamic acid significantly alleviated the intestinal structural damage of mice caused by high-salt diet and reduced the secretion of inflammatory cytokines in small intestine.While pyroglutamic acid inhibited the significant increase in the abundance of some flora caused by high-salt diet,such as Bacteroides,Alistipes and other microorganisms.In addition,pyroglutamic acid also restored and increased the abundance of flora that had been reduced during high-salt diet,including Candidatus Arthromitus,Bifidobacterium,Lactobacillus and other probiotics.In summary,pyroglutamic acid has a significant beneficial effect on high-salt diet mice,which can stabilize the intestinal structure,reduce the level of intestinal inflammation,and improve the intestinal flora structure and abundance.
  • GUO Feng, DONG Minghui, GAO Mengyuan, SHU Fang, SUN Dongdong, WANG Weiyun
    Food and Fermentation Industries. 2021, 47(2): 109-113. https://doi.org/10.13995/j.cnki.11-1802/ts.024679
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    To expand natural food additives in replacement of chemicals,steam distillation was applied to extract essential oil of lemon balm,followed by gas chromatography-mass spectrometry(GC-MS) to clarify components of the essential oil.After dilution,an antimicrobial screening was performed with three pathogenic bacteria (Staphylococcus aureus,Escherichia coli,Micrococcus tetragenus) using kirby-bauer method.IC50 was determined by dilution plating procedure subsequently.The results showed that the 25.91% of lemon balm essential oil were aliphatic compounds,20.16% were aromatic compounds and 53.93% were terpenoids.The lemon balm essential oil showed the weakest inhibition on S.aureus and the strongest inhibition on M.tetragenus.The inhibition intensity increased with the concentration of essential oil.The IC50 of lemon balm essential oil to M.tetragenus was 457 μg/mL.The essential oil of lemon balm had strong inhibitory effect on M.tetragenus,and the antimicrobial components were caryophyllene oxide,citronellal and 2,6-octadienal,3,7-dimethyl.
  • LI Zhengtao, NI Yongpei, XIANG Xingben, CAO Rongsheng, ZHAO Jinsong
    Food and Fermentation Industries. 2021, 47(2): 114-120. https://doi.org/10.13995/j.cnki.11-1802/ts.024891
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    Aging is an essential process of Luzhou-flavor Baijiu (Chinese liquor),the special environment of cave is beneficial to the aging of Baijiu.Cave aging Yingjiagong liquor stored at different storage years and locations were studied,the changes of its trace components were determined by gas chromatography,sensory analysis and physicochemical indexes.The results showed that,with the increase of aging time,the content of esters,aldehydes and ketones decreased,while the content of acids and alcohols increased ,there were differences in acids,esters,alcohols and aldehydes in different aging positions.This study reveals the effect of cave storage on the aging process of Luzhou-flavor Baijiu.It has certain guiding significance to optimize the cave aging of Luzhou-flavor Baijiu and improve liquor quality.
  • LI Chongyang, XIE Shengli, MA Liang, HOU Yong, FU Yu, ZHANG Yuhao
    Food and Fermentation Industries. 2021, 47(2): 121-129. https://doi.org/10.13995/j.cnki.11-1802/ts.024731
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    The effects of pH,ionic strength and TG enzyme treatment on the gel property of insoluble silkworm pupa protein were investigated.Fresh silkworm pupa was used as raw material to separate insoluble protein,and its amino acid composition was determined.After the treatment with different pH,ionic strength and TG enzyme,it was measured by rheology and Raman spectroscopy.The results showed that the hydrophobic amino acid ratio of insoluble silkworm pupa protein reached 40%.Rheological scanning showed that the insoluble silkworm pupa protein gel was a weak gel in the range of pH 5.0-8.0 and salt concentration 0-35 g/L after different pH and ionic strength treatment.And the G′ value and G″ value of the system were the highest,and the viscoelasticity was the best with the treatment of pH 6.0 and salt concentration was adjusted to 35 g/L.Moreover,the increases of pH led to the increase of protein structure ordering,but was not conducive to the formation of protein network.So G′ value and G″ value were the lowest at pH 8.0.Tryptophan residues tended to be embedded at pH 6.0 and 35 g/L salt concentration,while tyrosine residues were exposed at least at pH 6.0 and 35 g/L salt concentration,and G′ and G″ values were the highest.After adding TG enzyme,the modulus of G′ and G″ of the system increased obviously,and the viscoelasticity was enhanced.The experimental results could provide a theoretical reference for the processing suitability of insoluble silkworm pupa protein.
  • CHEN Ru, ZENG Lingying, LI Hongjun, WANG Chunxing, HE Zhifei
    Food and Fermentation Industries. 2021, 47(2): 130-136. https://doi.org/10.13995/j.cnki.11-1802/ts.025131
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    The objective of this study is to investigate the effect of composite adhesive (0.86% β-glucan,and 1.5% sodium caseinate,0.25% glucose -δ- lactone) on the quality of rabbit steak during the restructuring process.The hind legs of Ira rabbit and pork were used as raw material to produce restructured rabbit steak.The rabbit steaks were divided into the treatment and control group according to whether to add composite adhesive.The basic physical and chemical properties,quality and structure characteristics,rheological properties,and the microstructure of rabbit steak were observed at 0,6,12,18,24 h.The results showed that,compared with the control group,the content of protein and ash,the gel strength and the hardness of treatment group increased significantly (P<0.05).However,the fat content decreased significantly (P<0.05).In the treatment group,the moisture content,pH,elasticity,cohesiveness,adhesion and mastication of treatment group were less affected by the restructuring time.Moreover,the frequency and dynamic temperature scanning showed that compound adhesive could enhance the gelatinous rheological properties of rabbit meat steak during the recombination process,while the recombination thermal stability of rabbit meat steak was poor at higher temperature.Furthermore,the results of scanning electron microscope showed that the uniform,dense and narrow space structure of reconstituted rabbit steak were better maintained by the addition of composite adhesive.Hence,the quality characteristics of the recombined rabbit steak was improved by composite adhesive,and the results provided a reference for the application of composite adhesive.
  • FANG Jun, ZHOU Jingwen, ZENG Weizhu
    Food and Fermentation Industries. 2021, 47(2): 137-144. https://doi.org/10.13995/j.cnki.11-1802/ts.024665
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    In order to improve the fermentation intensity of α-ketoglutaric acid (α-KG) with Yarrowia lipolytica,a high α-KG production strain 1-C6 screened previously was treated by iterative mutagenesis,and the fermentation conditions for α-KG production with the final obtained mutant were systematically optimized.Firstly,based on the previous established high-throughput screening method,a high-yield mutant 4-E2 was obtained by iterative mutagenesis with ethyl methane sulfonate,nitrosoguanidine and ultraviolet light.Compared with the starting strain 1-C6,the production of α-KG was enhanced by 56.7%.Then,effects of agitation speed,ventilation ratio,addition of sodium acetate and CaCO3 on the accumulation of α-KG with mutant 4-E2 were investigated in 3 L fermenter.It was found that a two-stage ventilation ratio combined with relative higher rotating speed was more suitable for α-KG production,and the optimum addition of sodium acetate and CaCO3 were 6 g/L and 10 g/L,respectively.On this basis,a strategy of constant feeding strategy was further determined after exploring different strategies of fed-batch fermentation.Under the optimal conditions,the production of α-KG reached 75.86 g/L,which was 44.4% higher than that of the batch fermentation.The results could provide references for the industrialization of α-KG fermentation.
  • LI Yanhong, WANG Wenhang
    Food and Fermentation Industries. 2021, 47(2): 145-152. https://doi.org/10.13995/j.cnki.11-1802/ts.025056
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    Yak beef with high nutritional value is difficult to be accepted by the elder who suffer from the physiological decline and swallowing disorders for its poor chewing.The objective of this study was to explore the effects of different heating temperatures (55,65,75,85 ℃) and time (20,30,40 min) on the physicochemical properties and sensory characteristic of yak meat.Results showed that,with the increase of heating temperature and the extension of heating time,cooking resulted in the meat significantly loss (P<0.05) and the water content decreased (P<0.05).Moreover,the pH value increased and the muscle fiber contracted significantly by SEM.Besides,according to the texture analysis,the hardness,springiness,chewiness and resilience of sample increased with increasing temperature,however,it decreased firstly and then increased with the extension of time.Combined with sensory evaluation and the above results,the best physicochemical properties and sensory quality of yak meat would be 75 ℃ for 30 min.The results demonstrated that low-temperature and a relative long time heating had a significant effect on the quality of yak meat,which provided theoretical basis and technical support for the development of meat-based food for the aged.
  • ZONG Yuan, LIU Dengfeng, LIU Yian
    Food and Fermentation Industries. 2021, 47(2): 153-159. https://doi.org/10.13995/j.cnki.11-1802/ts.024368
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    For identifying the model parameters of rice wine fermentation process based on the Levenberg-Marquardt method,it is easy to fall into local optimum and slow to converge.This paper proposed an enhanced ant lion optimization algorithm called LCALO (ant lion optimization with Levy flight and Cauchy mutation,LCALO),which employed Levy flight and Cauchy mutation to overcome this problem.Levy flight could improve the global search ability of the algorithm,and the Cauchy mutation with a long tail helped trapped ant lions escape from local optima.The results showed that compared with the genetic algorithm,the particle swarm algorithm and the ant lion algorithm,the LCALO had the advantages of faster convergence speed,better global search ability,and local development ability.Finally,the improved algorithm was applied to the parameter identification of a rice wine fermentation model.Simulation results proved that the algorithm had good identification ability.
  • LI Zhe, ZHANG Suyi, YANG Hongjun, QIN Hui, YUAN Ling, YANG Ping, AO Zonghua, SONG Pan
    Food and Fermentation Industries. 2021, 47(2): 160-166. https://doi.org/10.13995/j.cnki.11-1802/ts.024888
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    The solid waste produced in brewing process,including sludge and distilled grains,is about three times of liquor.To investigate the conversion of the solid wastes discharged from Luzhou Laojiao Co.,Ltd.into an organic fertilizer by thermophilic composting,sorghum was supplied with the organic fertilizer to evaluate the fertilizer efficiency.The results showed that the mixture of sludge,distilled grains and decomposable inoculant was subject to heating,high temperature and cooling stages in the composting process.Concomitantly,nitrogen,phosphorus,potassium and humic acid in the compost substrates increased with moisture decreased while the substrate color changed from tawny to brown,black-brown or black.The mixture of sludge and distilled grains at the rate of 1∶2 in weight contained 65% moisture with about 25∶1 of C∶N ratio and pH 6.5 composted most rapidly.The organic fertilizer produced had high quality and fitted the National Organic Fertilizer Quality Standard (NY525-2012).The application of this organic fertilizer (OF) in combination with chemical fertilizers (CF) at the proportion of 1∶1 in the terms of nitrogen increased sorghum yield by 12.55%,grain nitrogen content by 36.70%,plant phosphorus uptake by 22.22% and potassium uptake by 29.20%,respectively,compared with those of CF alone.Besides,there were higher available nitrogen and enzyme activities (including phosphatase,urease and dehydrogenase) in the soil supplied with OF+CF than those of sole CF.Taken together,the mixture of sludge and distilled grains at an adequate proportion converted into an organic fertilizer through thermophilic composting,which fitted the National Standard.The application of OF plus CF at a proper rate increased sorghum yield,improved plant nutrient,and stimulated soil enzyme activity.
  • ZHAO Ziyue, ZHANG Yinong, PENG Songlin, LI Yixuan, KANG Mengyao, SHANG Yongbiao
    Food and Fermentation Industries. 2021, 47(2): 167-173. https://doi.org/10.13995/j.cnki.11-1802/ts.024825
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    The effect of ultrasonic power on soybean oil pre-emulsification was investigated and the mechanism of ultrasonic treatment was discussed.A novel method to improve the quality of emulsified meat products was explored.Pure water was mixed with soybean oil at the ration of 1∶1 with the addition of 2 g/L sodium caseinat and emulsified homogeneity for 3 min with ultrasonic intermittents intermittently,and then the indicators including emulsification activity and apparent stability,emulsion viscosity were measured.The results showed that ultrasonic treatment had a significant effect on the emulsifying activity and stability of soybean oil pre-emulsion.And the emulsification performance was the best which reached 59.95 m2/g when the ultrasonic power was 360 W.With the treatment of ultrasonic,the viscosity of emulsion was increased,and the particle size of emulsion was decrease.Besides,the adsorption capacity of protein on the surface of emulsion was also increased which enhanced the emulsification effect.Ultrasonic treatment was an effective means to increase the emulsification and emulsification stability of pre-emulsifiers.
  • ZHAO Zhijun, ZHANG Yanzhu, LIU Yanbo, ZHOU Pingping, GE Shaohua, SUN Xiyu
    Food and Fermentation Industries. 2021, 47(2): 174-181. https://doi.org/10.13995/j.cnki.11-1802/ts.024958
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    In order to obtain bacteria with high esterase production,combinatorial mutagenesis using ultraviolet (UV) and diethyl sulfate (DES) technology was applied to previous isolated strain Bacillus velezensis Nz 1.201.The high-yielding mutant strain D27 was selected by the transparent circle method for primary screening and the esterase activity measurement for rescreening.After five consecutive subcultures,its genetic performance was relatively stable.The enzyme activity reached 62.45 U/100 g,which was 66% higher than that of the original strain.Mutant strain D27 was further used in solid-state fermentation.Based on single factor experiment,Box-Behnken design-response surface methodology was used to optimize culture conditions for mutant D27 with esterase activity as the response value.The results showed that the optimal culture conditions were:33℃,6 days,strain inoculation volume of 3%,and raw material moisture content of 73%.Under the optimal condition,the highest esterase activity was 89.52 U /100 g,an increase of 137% compared with the initial strain.Studies have shown that the mutant strain D27 has a higher ability to produce esterase under solid-state fermentation,and has certain application potential in liquor production.
  • SHI Jia, ZHANG Hongmei, SU Zijie, XU Fang, YU Xiang, FENG Yanli
    Food and Fermentation Industries. 2021, 47(2): 182-187. https://doi.org/10.13995/j.cnki.11-1802/ts.024765
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    To explore the key components in soybean powder which promotes monacolin K (MK) production and screen the alternatives to soybean powder for the production of functional red yeast rice with high MK content,the influences of classical carbon source,glucose,and substrates from soybean on MK production were analyzed using Monascus pilosus MS-1 as the experimental strain and rice as the main fermentation substrate.The results indicated that MK production was enhanced significantly when 3% soy isoflavone or 5% soy protein isolate was added to the substrate (P<0.05).MK production was significantly inhibited when 3% glucose was added to the substrate (P<0.05).However,3% glucose and 5% soy protein isolate showed significantly synergistic effect on MK production when they were used simultaneously (P<0.05).It was more efficient for producing MK when 3% glucose and 5% soy protein isolate were added to the substrate simultaneously.The MK yield were 1.53 and 16.80 times as that of adding soybean powder and control,respectively.The results provide theoretical and technical support,as well as using glucose and soy protein isolate as an alternative to soybean powder for the production of functional red yeast rice with high concentration of MK.
  • LI Hui, NIE Congning, XIONG Bingquan, TANG Qian, HUANG Li, DU Xiao, YANG Yi, PENG Lingli, LI Qian
    Food and Fermentation Industries. 2021, 47(2): 188-195. https://doi.org/10.13995/j.cnki.11-1802/ts.024660
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    In order to study the effect of shaking technology on the aroma quality of “Chongqing Pipa tea” black tea in Sichuan province,the fresh leaves (Pipa) of wild arbor type “Chongqing Pipa tea” were used as raw materials in this study.Compared with the processing technology of traditional black tea in Sichuan Province,the shake-green technology of Oolong tea was used to process “Chongqing Pipa tea” for 20 min (CP1),40 min (CP2) and 60 min (CP3).The composition,content,sensory quality,aroma characteristics and relative odor activity value of black tea aroma were analyzed.The results showed that 10-11 aroma compounds were found in the “Chongqing Pipa tea” black tea compared with controlled groups.Moreover,the sensory quality of aroma of “Chongqing Pipa tea” black tea could be significantly improved by the new technology of shake-green,which showed that:CP1 had an outstanding aroma of fruity,roasted and sweet;CP2 had a strong aroma of sweet,floral and woody,and CP3 had a rich aroma of fruity;the aroma compounds of isopentaldehyde,isobutyraldehyde and 2-methyl butyraldehyde may be the main aroma components in the aroma of “Chongqing Pipa tea” black tea.So the “Chongqing Pipa tea” black tea processed by the shake-green technology had excellent aroma quality,which could provide theoretical support for the actual production.
  • JIANG Feifeng, TAN Xiaohui, HU Penggang, PAN Xuemei, YAN Jin
    Food and Fermentation Industries. 2021, 47(2): 196-204. https://doi.org/10.13995/j.cnki.11-1802/ts.025208
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    In this study,pomelo peel was used as raw material,ultrasonic-microwave collaborative extraction technology was used to extract pomelo peel polysaccharide.The optimal extraction process was optimized in response to the surface.Decolorization and deproteinization of pomelo peel polysaccharide were carried out,and their structure and antioxidant activity were analyzed experimentally.The results showed that the optimum process of ultrasonic-microwave collaborative extraction of pomelo peel polysaccharide was as follows:Microwave power 400 W for 34 min with liquid-solid ratio 39∶1 (mL∶g) at pH 9.0.Under above conditions,the yield of polysaccharide reached 10.41%.Moreover,Uv-spectrum analysis showed that there was no protein and nucleic acid residue.Gel permeation chromatography was used to determine the average molecular weight of pomelo peel polysaccharide to 2.4×104 Da.The results of LC-MS showed that the polysaccharide was acid heteropolysaccharide which consisting of Mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,arabinose with a molar ratio of 0.502∶0.960∶0.295∶6.331∶40.673∶10.732∶7.923.Furthermore,scanning electron microscope and infrared spectrum analysis showed that the polysaccharide was mainly irregular fragment structure,accompanied by some small irregular particles,the surface of the fragment was rough,and the resulting polysaccharide presented irregular shape,indicating that the polysaccharide had amorphous structure and was a pyranoid glycoside ring skeleton.The results of antioxidant activity showed that pomelo peel polysaccharides had better free radical scavenging effect,and the scavenging rates of DPPH free radical,hydroxyl free radical and ABTS free radical were 55.58%,46.32% and 40.25%,respectively,when the mass concentration was 1.0 mg/mL.The results of this experiment provide a basis for further separation and purification,structural characterization,bioactivity research of pomelo peel polysaccharides and development of functional food with potential.
  • XIONG Tian, HE Jianjun, CAI Fang, WANG Shaohua, SHI Jianbin, CAI Sha, SUI Yong, CHEN Xueling, FAN Chuanhui, MEI Xin
    Food and Fermentation Industries. 2021, 47(2): 205-211. https://doi.org/10.13995/j.cnki.11-1802/ts.024407
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    To improve the quality and stability of potato hot dry noodles,the effects of the amount of wheat gluten on the characteristics of mixed flour and the quality characteristics of fresh and cooked potato hot dry noodles,such as cooking,texture and storage,were studied based on the comprehensive analysis of basic components and physicochemical properties of wheat gluten.The results showed that wheat gluten has poor solubility and water retention,but good foaming and emulsifying properties.With the increase of gluten content in the mixed powder,its water holding capacity decreased and its water absorption and swelling capacity increased.Moreover,with the increase in the amount of wheat gluten,the water absorption of hot dry potato noodles showed a downward trend,the cooking loss rate increased first and then decreased.Besides,the shear and tensile properties of fresh wet and cooked noodles decreased first and then increased,and the shear and tensile properties of the fresh and wet potato hot dry noodles were higher than those of the cooked potato hot dry noodles when the amount of gluten was the same.The optimal addition amount of gluten is 10%-15%,under which the shear force of the potato hot dry noodle was the smallest,the tensile force and viscosity were moderate with good texture properties.The quality stability of potato hot dry noodles could be improved by storage after cooking.
  • ZHANG Zhaoyun, ZHOU Qiping, GONG Qifu, ZHAO Baotang, SHANG Qi, ZHANG Zhihua, YANG Fumin
    Food and Fermentation Industries. 2021, 47(2): 212-219. https://doi.org/10.13995/j.cnki.11-1802/ts.024713
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    The aim of this study was to investigated the technological conditions of ultrasonic assisted extraction and the characteristics of albumin from “Longli 1” seeds.The new quinoa variety “Longli 1” was used as raw material,and the albumin was extracted by ultrasonic assisted extraction after defatted and air-dried.Based on the single factor tests of different ultrasonic power,solid-liquid ratio,ultrasonic time and water bath temperature,the extraction process conditions of the quinoa albumin from “Longli 1” were optimized by the response surface tests of four factors and three levels.And its amino acid composition,molecular weight distribution and oxidation resistance were measured.The results showed that under the conditions of ultrasonic power 354 W for 40 min at 36.9 ℃ and with the solid-liquid ratio 1∶25.5 (g∶mL),the extraction rate of quinoa albumin could reach 64.11 mg/g.Albumin extracted from “Longli 1” contained 17 kinds of amino acids,and cysteine was the first limiting amino acid.The molecular weight determination results showed that albumin subunits were mainly distributed in 15 kDa-25 kDa and 25 kDa-35 kDa.When the albumin concentration was 1.2 mg/mL,the clearance rate was 46.34% and 67.15%,respectively.The results provided a basis for the extraction and utilization of albumin from quinoa seeds.
  • DONG Yining, LI Yu, HUANG Kaijun, JIA Sheng, SONG Zhiwen
    Food and Fermentation Industries. 2021, 47(2): 220-225. https://doi.org/10.13995/j.cnki.11-1802/ts.024829
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    To evaluate the effect of Chuzhou chrysanthemum addition on liquor product,Chuzhou chrysanthemum was soaked into the liquor to enrich the total flavonoids,and the galacto-oligosaccharide (GOS) was added as a sweetener.The flavonoids-enriched low alcohol Chuzhou chrysanthemum-soaked liquor was developed by ultrasonic soaking at low temperature.The content of total flavonoids was used as quality index,and the optimum conditions were as follows.The ratio of dried Chuzhou chrysanthemum to base liquor was 1∶50 (g∶mL).The pre-soaked liquor was then subjected to extraction for 25 min in ice bath (0℃) under 160 W ultrasonic.After storage for 48 h,the alcohol content of the pre-soaked liquor was reduced to 8%vol.At last,the galacto-oligosaccharide was added to the low alcohol Chuzhou chrysanthemum-soaked liquor to reach a concentration of 50 mg/mL.The total flavonoid content in the product obtained by the optimized process reached 79.51 mg/g,and the retention of GOS reached 98.47%.The scavenging rate of 2,2-diphenyl-1-picylhydrazyl (DPPH) free radical was 88.86%,and the scavenging rate of superoxide anion (O-2·) free radical was 79.91%.The results showed that the low alcohol Chuzhou chrysanthemum-soaked liquor had high total flavonoids content and significant antioxidant activity in vitro,which has the potential for functional food development.
  • XIA Xinke, ZHANG Yuane, LEI Shengjiao, HU Biao, CHEN Yuting, FU Caixia
    Food and Fermentation Industries. 2021, 47(2): 226-232. https://doi.org/10.13995/j.cnki.11-1802/ts.024957
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    In order to screen out a high naringinase yielding mutant and explore its industrial application value,a naringinase producing strain Aspergillus tubingensis MN589840 was isolated from the mildewed pomelo peel.Then,a naringinase producing mutant UA13 was screened by the combination of UV irradiation and atmospheric and room temperature plasma (ARTP).The naringinase activity of UA13 was 41.524 U/mL,which was 3.06 times of that of MN589840.The results of naringinase hydrolysis conditions showed that the optimum temperature range was 40~50 ℃,and the optimum pH was 5.0.The naringinase produced by UA13 was used to debitter Yichang tangerine juice and the physical and chemical indexes of juice were detected.The results showed that the content of naringin in juice was lower than the threshold with the hydrolysis time of about 50 min,and only a small amount of naringin was left in the juice after 75 min of hydrolysis.High performance liquid chromatography (HPLC) was used to test the debittering results.The naringinase produced by UA13 could hydrolyze naringin effectively and improve the flavor of juice.Therefore,the mutant UA13 has industrial application potential,which lays a foundation for further research.
  • WANG Dongdong, BAO Yongbi, GUAN Rui, TANG Yao, MING Jianying, LI Jiayi, HUANG Runqiu, CHEN Gong, ZHANG Qisheng
    Food and Fermentation Industries. 2021, 47(2): 233-240. https://doi.org/10.13995/j.cnki.11-1802/ts.024954
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    To study the effect of temperature on the flavor of cabbage Paocai (Chinese pickle) during fermentation,the organic acids,amino acids,and volatile components of Paocai at 10 ℃,15 ℃,25 ℃,and 35 ℃ were analyzed by high performance liquid chromatography and headspace solid phase microextraction coupled with gas chromatography-mass spectrometry.Principal component analysis was used to determine the important volatile components of Paocai liquid.The results showed that in the range of 10-35 ℃,increasing the fermentation temperature was conducive to the rapid acid production of Paocai and shortened the maturation period.Temperature was positively correlated with lactic acid and acetic acid,and negatively correlated with citric acid,malic acid and amino acids.Glycine,threonine,and proline was dominant in Paocai liquid fermented at 10 ℃.Alanine and arginine were dominant in the remaining Paocai.The high temperature fermented Paocai liquid had more volatile components.The important volatile components of the fermented Paocai liquid at 10 ℃,15 ℃,25 ℃ and 35 ℃ were 1-pentanol,hexanal;allyl isothiocyanate,cedrol,2,4-di-tert-butylphenol;cedrol,3,5-octadien-2-one,2,4-heptadienal,hexanal;phenethyl isothiocyanate and cedrol.Low temperature was good for preventing rancidity of Paocai,and high temperature prolonged fermentation was prone to spoilage,which produced a lot of acetic acid.The results provide data support for the production and processing of Paocai.
  • XU Wenwen, LIANG Yulin, WANG Yunxia, LIU Xiu, YIN Jianjun, ZHOU Guangjun, SONG Quanhou, DING Mengxuan, ZHOU Pengfei
    Food and Fermentation Industries. 2021, 47(2): 241-246. https://doi.org/10.13995/j.cnki.11-1802/ts.024936
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    The aim of this research is to establish a loop-mediated isothermal amplification (LAMP) method that can simultaneously detect Salmonella and Staphylococcus aureus in milk powder.LAMP primers were designed based on the conserved region of invA gene in Salmonella and the nuc gene in S.aureus.After primer concentration optimization and melting curve analysis to determine the source of amplification target,a double LAMP detection method for simultaneous detection of Salmonella and S.aureus was established.The results showed that when the primer concentration ratio of Salmonella to S.aureus was 3∶1,the established double detection system had the best amplification efficiency.The detection system had good specificity and high sensitivity.Six target strains and nine non-target strains were tested,resulting in no false positive and false negative result.The sensitivity of the two foodborne pathogens reached 102 fg/μL.In addition,according to the annealing temperature in the melting curve,the target strain can be accurately judged to complete the strain differentiation.The established detection method can be used for rapid on-site screening of Salmonella and S.aureus in milk powder samples of dairy companies.
  • ZHU Wenzheng, YAN Shunyang, XU Yan, WANG Qiuyu, ZHANG Man, ZHANG Huimin, ZHOU Xiaoyan, YANG Zhangping
    Food and Fermentation Industries. 2021, 47(2): 247-253. https://doi.org/10.13995/j.cnki.11-1802/ts.025351
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    This article explored the effect of cooking time on the volatile flavors of braised pork,and solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was applied to volatilize.Sexual flavor substances were separated and identified.The main flavor components were calculated by adding internal standard substances,and combined with odor activity value (OAV),the key flavor substances of braised pork in the cooking process were discussed.Studies have shown that 93 kinds of volatile flavor compounds have been identified in braised pork during the cooking process and cooking time had a significant effect on the volatile flavor compounds of braised pork.Flavor compounds such as hexanal and nonanal were cooked in braised pork for 90,120 and 150 min were the most important flavor substances.When cooking for 120 minutes,aldehydes,aliphatic hydrocarbons,and alcohols were contributed significantly to the flavor composition of braised pork.Furthermore,if cooked for 150 minutes,the content of hexanal reached 22.75 mg/kg,the content of nonanal reached 12.17 mg/kg,and the content of 2-pentylfuran was 7.60 mg/kg respectively.There were 9 kinds of braised pork with OAV value ≥1.According to the degree of flavor contribution,they were nonanal,methyl caproate,hexanal,2-heptenal,1-octen-3-ol,2-pentylfuran,trans-2-heptanone,2-pentanone,3-hydroxy-2-butanone.This research provides a certain theoretical basis for product development and control of braised pork.
  • ZHANG Pei, WANG Yinhong, LI Gaoyang, SHAN Yang, ZHU Xiangrong
    Food and Fermentation Industries. 2021, 47(2): 254-259. https://doi.org/10.13995/j.cnki.11-1802/ts.025288
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    The chilling injury of juicy peach caused by low temperature was identified and analyzed by near infrared (NIR) spectroscopy.In this paper,two-classification and multi-classification models of different chilling injury stages of juicy peach were established during low temperature storage,and the effects of different spectral pretreatment methods on the model were discussed.The classification performance of partial least squares discriminant analysis (PLS-DA),principal component discriminant analysis (PCA-DA),K-nearest neighbor (K-NN) and SIMCA modeling methods were compared.The results showed that the performance of PLS-DA model was the best,and the total accuracy of two-classification model and multi-classification model were 0.93 and 0.71,respectively.The two-classification model could be used for rapid and accurate identification of cold injury browning,while multi-classification model could be used for preliminary screening of different chilling injury stages of juicy peach during low temperature storage.
  • YUE Cuinan, QIN Dandan, CAI Hailan, WANG Zhihui, LI Chen, LI Yansheng, YANG Puxiang
    Food and Fermentation Industries. 2021, 47(2): 260-267. https://doi.org/10.13995/j.cnki.11-1802/ts.024971
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    The aim of this study was to explore the taste characteristics and molecular sensory basis of Congou black tea in northern Jiangxi province.Quantitative description analysis and liquid chromatography-mass spectrometry were used to evaluate and detect the taste attributes and non-volatile compounds of 11 samples of Congou black tea.Then,the key compounds which played important role in the taste attributes were screened by partial least square-variable import project.The results showed that nine taste descriptors and definitions of Congou black tea in northern Jiangxi Province were obtained,and the main taste characteristics were sweetness,mellowness and sweet after taste.Among them,bitterness was positively correlated with astringency,and negatively correlated with sweetness.And,sweetness was negatively correlated with tainted taste.Besides,umami was found to be positively correlated with sweet after taste.Moreover,seventy four kinds of nonvolatile compounds were screened by LC-MS which including 25 phenols,23 amino acids,9 pigments,9 organic acids,5 sugars,2 alkaloids and tea saponins.The results of PLS-VIP revealed that the VIP values of 33 compounds were greater than 1,including 10 amino acids,12 phenols,3 pigments,1 alkaloid,4 acids and 3 sugars,which indicated that these compounds were the key components contributing to the taste of Congou black tea in northern Jiangxi Province.This paper determined the taste characteristics of Congou black tea in northern Jiangxi Province and its important compounds,which provided theoretical basis for further study on the directional regulation of taste quality of Congou black tea in northern Jiangxi province.
  • WEI Jinmei, LIU Caiyun, FANG Yan, JIAN Naidan
    Food and Fermentation Industries. 2021, 47(2): 268-273. https://doi.org/10.13995/j.cnki.11-1802/ts.025053
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    This study determined fatty acids compositions and the contents of 8 trace elements in seabuchthorn fruit oil.The oil was obtained by subcritical fluid extraction.Fatty acid compositions were determined by gas chromatography-mass spectrometry (GC-MS) after fatty saponification and fatty acid methyl esterization.It was quantified by using internal standard method to determine the content of fatty acids in the oil.And the trace elements were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) after microwave digested in nitric acid.Eight trace elements were quantified by using their standard reference materials respectively.The results showed that a total of 19 fatty acids were detected.And the amount of saturated,monounsaturated and polyunsaturated fatty acids were 24.42%、47.26% and 28.31%,respectively.The contents were higher than others that they were 39.71、44.29、48.15、35.25 and 35.25 mg/g of C16∶0、C16∶1、C18∶1、C18∶2、C18∶3 in the oil,respectively.In addition,the detection limits were 0.5、0.25、0.125、0.1、0.1、0.1、0.2 and 0.4 μg/g of K、Na、Ca、Mg、Cu、Zn、Fe and Mn,respectively.Moreover,the average recovery of each element was 85.0%-94.4%,and the relative standard deviation was 3.28%-9.23% (RSD<10%).And the contents were higher than others that they were 149.28、65.29、41.08、21.27、14.43 and 4.71 μg/g of Na、Ca、K、Mg、Fe and Zn in the oil,respectively.The contents of unsaturated fatty acids and trace elements were riched in seabuckthorn fruit oil so that the oil was nutritious.The results provided basic data for the further development and utilization of seabuckthorn.
  • ZHANG Mengqin, XU Lu, SUN Yazhen, SHI Jianhui
    Food and Fermentation Industries. 2021, 47(2): 274-278. https://doi.org/10.13995/j.cnki.11-1802/ts.024985
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    To investigate the correlation between the nutrient contents of different species of Chinese rose petals,Kjeldahl nitrogen determination,inductively coupled plasma atomic emission spectroscopy (ICP-AES),and acid hydrolysis were employed to measure the principal nutrient contents in ten species of Chinese rose petals from Nanyang in Henan.Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were then utilized to evaluate each nutrient.Using these methods,the average protein and fat levels across ten different species were 8.93% and 1.28%,respectively.The average contents of mineral elements K,Ca,Mg,Na,Fe,Mn,Cu,Zn,Se were 14 459.41,2 171.43,1 692.21,53.04,93.31,30.98,3.70,20.89,0.87 μg/g.However,there were statistically significant differences in the concentration of protein,fat,macroelements,and microelements across species.Moreover,the coefficient of variances of difference species were significantly different.PCA of each nutrient content resulted in a four-factor model,and the cumulative contribution rate of the four principal components was 83.24%.The ten species were categorized into 5 groups by PCA,which was consistent with the result obtained using HCA and indicating that PCA and HCA were suitable methods for classification of different Chinese rose species.The study thus provides scientific references and valuable insights for germ plasm screening,quality evaluation,integrated utilization,and product development of Chinese roses.
  • XIA Xuanze, LI Yan, QIAN Haifeng, ZHANG Hui, QI Xiguang, WANG Li
    Food and Fermentation Industries. 2021, 47(2): 279-284. https://doi.org/10.13995/j.cnki.11-1802/ts.024730
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    Pea protein is a good plant protein,which is hypo-allergenic,as well as balanced amino acid ratio and high nutritional value.The emulsibility is an important property of protein.However,the emulsibility of natural pea protein is not good enough for its application in food industry.Recently,physical,chemical and enzymatic treatments were used to improve the emulsibility of pea protein.The composition and emulsibility were introduced in this paper.Meanwhile,the effect of modification on the structure and emulsification property of pea protein was also summarized.Furthermore,the problem in the pea protein industry was discussed.And the research emphasis of pea protein emulsification in the future is prospected,which is helpful for further development and application of pea protein in food industry.
  • WU Han, SHI Wenzheng, WANG Yixin, YANG Wenxian
    Food and Fermentation Industries. 2021, 47(2): 285-291. https://doi.org/10.13995/j.cnki.11-1802/ts.024947
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    Salting is one of the main processing methods of fish preservation.Compared with frozen,it requires less equipment,and can produce unique flavor,so salting has a great business application potential in this filed.In the process of salting,the flavor and physicochemical properties of fish will change greatly because of microbial activities,endogenous enzyme and salt.Detecting the changes can provide theoretical basis for regulating and improving product quality,guiding enterprise production and standardizing market operation.The volatile and non-volatile flavor compounds of fish and the changes of some physicochemical properties in the process of salting were reviewed,and the development direction of salting fish was prospected.
  • WEN Chengkun, LI Qin, CAI Kaiyun, CHEN Ping, GUO Tingting, CHEN Maobin
    Food and Fermentation Industries. 2021, 47(2): 292-297. https://doi.org/10.13995/j.cnki.11-1802/ts.024678
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    Ferulic acid esterase (FAE) is an enzyme with potential application,which can be widely found in nature and belongs to a subclass of carboxylic acid ester hydrolase.After a series of reactions,it can release phenolic acid,ferulic acid (FA) and p-coumaric acid from natural hemicellulose and pectin.It is used to obtain several different products,such as human and animal food,pulp and paper,fuel ethanol and pharmaceutical preparations.This paper reviews the enzymatic characteristics,production,structure analysis and potential applications of FAE.The aim is to accelerate scientific research of ferulic esterase driven by industrial demand,promote knowledge transfer,realize efficient utilization of such material,and contribute to the transformation of plant biomass and the production of high value-added molecules biosynthesis.
  • ZHANG Qiang, LI Weihua
    Food and Fermentation Industries. 2021, 47(2): 298-304. https://doi.org/10.13995/j.cnki.11-1802/ts.024999
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    Antioxidant peptides show great potential in the fields of food,health products,medicine and cosmetics.In this paper,the preparation,purification,structure identification,molecular modification,antioxidant activity evaluation and mechanism of antioxidant peptides were reviewed,the applications of in silico tools in the research of antioxidant peptides were analyzed and summarized.The problems existing in the research and development of antioxidant peptides were pointed out,and the development prospect of antioxidant peptides was outlooked,aiming to provide a reference for their further development and utilization.
  • ZHANG Mingjuan, WANG Juan, YUAN Lei, XIAO Zhaojing, LONG Mei, LI Genrong
    Food and Fermentation Industries. 2021, 47(2): 305-310. https://doi.org/10.13995/j.cnki.11-1802/ts.024585
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    Multiplex PCR technology is developed on the basis of conventional PCR technology,its reaction principle,reaction reagent and operation process are the same as conventional PCR.The difference is that multiplex PCR technology adds two or more pairs of primers in the same reaction system to amplify different templates and get different target fragments.Multiplex PCR technology has the advantages of high throughput and cost saving in the detection of foodborne pathogens,so it has a good development prospect in the actual detection.This paper summarizes the research progress of multiplex PCR technology in the detection of foodborne pathogens and its current situation in standard formulation and commercial application,and puts forward the technical points and future research directions of multiplex PCR technology in the detection of foodborne pathogens.
  • GAO Tao, TANG Huali, SUN Guiju, LIANG Xiai, LUO Huangyang
    Food and Fermentation Industries. 2021, 47(2): 311-321. https://doi.org/10.13995/j.cnki.11-1802/ts.024908
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    This paper briefly introduces the development status of Chinese health food and the policy changes in various aspects since the reform of the dual-track system.And it systematically analyzes the problems existing in the product,market,regulatory approval,and other aspects of Chinese health food.It is found that the research on the efficacy of health food is mainly focused on enhancing immunity.In addition,for regulatory agencies,with the improvement of health food policies in registration and filing,raw and auxiliary materials management,function claims,product naming and publicity,the main problem of health food has changed from the stage of no legal basis to the stage of how to effectively enforce the law.For consumers,it has become an important force to promote the development of the market to enhance the awareness of anti-fraud of health food and assist the regulatory authorities to strengthen the supervision and management of the health food market.It is believed that after this,the health food industry will enter rapid development period again.
  • XUE Shan, JIANG Wenhui, LI Bianhua, PAN Yutian, LIU Jingna, LI Hui, FEI Peng, HU Yonghua
    Food and Fermentation Industries. 2021, 47(2): 322-328. https://doi.org/10.13995/j.cnki.11-1802/ts.024311
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    Under the general background of the first-class undergraduate major construction,the major of Food Science and Engineering in Minnan Normal University(MNU) find its own school-running major orientation and development direction.The innovation mode of major construction implemented in MNU is summarized as "one foundation,two cores,three types,four combinations",that is taking serious measures on "one foundation (ideological and political education)",resonating "two cores (teaching staff and talent training)",building "three types"(teaching and research oriented,service-oriented,innovative),and paying attention to "four combinations"(teacher specialty,professional characteristics,subject characteristics,integration of innovation and entrepreneurship).Driven by this innovative mode,the specialty construction of Food Science and Engineering in our school has achieved fruitful practical achievements in the construction of teaching staff and talent training etc.Besides,in the followed-up construction,the major of Food Science and Engineering will hold the practical train of thought,adhere to the "one foundation"as the driving force,focus on the improvement of "two cores"as the key point,and take the promotion of "three types"as the foothold.In addition,we should strictly follow the standard and dare to reform and innovate,so as to provide reference and innovation impetus for the construction of food specialty.