YANG Jixia, ZENG Xiangping, PU Huimin, YANG Xiahe, HE Zhifei
Fu brick tea is a kind of post-fermented tea, and microorganisms play a critical role in the formation of its unique qualities. Shaanxi is the “birthplace” of Fu brick tea, and also one of the major producing areas. This study aimed to analyze the microbial profiles of Shaanxi Fu brick tea. Six Fu brick tea samples were collected from Shaanxi province, their 16S rRNA genes and ITS1 genes were sequenced with the use of Illumina MiSeq technology, and then were analyzed with Quantitative Insights Into Microbial Ecology (QIIME) pipeline. The results showed that only sx4 and sx5 contained bacteria and the dominant genera including Enterococcus, Lactobacillus, Akkermansia, Paracharacteroids and Faecalibacterium. The dominant fungi was Aspergillus, with an average relative abundance of 99.123%. At species level, one or more following species may involve in Fu brick tea samples which including Aspergillus amstelodami, Aspergillus chevalieri, Eurotium sp., Aspergillus spiculosus, Aspergillus hetrocaryoticus, Aspergillus sp., Aspergillus ruber and Aspergillus cristatus. Besides, species with lower abundance such as Aspergillus cibarius (4.138%), Aspergillus piperis (0.879%), Aspergillus penicillioides (0.019%) and Aspergillus gracilis (0.0058%) Candida, Cyberlindnera, Cryptococcus, Guehomyces, Mortierella, Humicola, Fusarium, Gibberella, Phoma were also be found in the collected samples. This study provided the basic diversity and community structure of microorganisms in Shaanxi Fu brick tea, and may help for the protection of Fu brick tea as the geographical indication products.
