Li Jun-lan, Yu Qun-li, Zhang Li, Guo Zhao-bin, Liu Liang-liang
The purpose of this paper is to optimize the conditions for extraction of pancreatic enzyme from the ovine pancreas.With low concentration of isopropanol for extraction and high concentration of isopropanol for precipitant,the effects of activating time,precipitant concentration,extracting pH and amount of CaCl2 on pancreatin preparation were studied by the means of uniform design on the basis of a single factor experiment.With the extracting rates of trypsin,amylase and lipase as responding results,the factors for pancreatin preparation were optimized by partial least square(PLS) regression analysis.The results showed that the optimal processing conditions for preparation of sheep pancreatic enzymes: activating time 12h,precipitant concentration 41%,extracting pH 6.34 and CaCl2 0.08%.Under the conditions,activities of trypsin,amylase and lipase were 3 062.781u/g,30.06 ku/g,32.37 ku/g respectively and the extract rate was 6.16%.The relative errors of the model values were 8.11%,1.28%,3.55%,5.30%,respectively.These showed the suitability of the mathematical quadratic polynomial regression model and the rationality of PLS optimization results.
