15 February 2019, Volume 45 Issue 3
    

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  • PANG Zhihua, CAO Jinnuo, ZHENG Yumeng, LUO Yuwan, LIU Xinqi, XIAO Lin
    Food and Fermentation Industries. 2019, 45(3): 1-6. https://doi.org/10.13995/j.cnki.11-1802/ts.018305
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    The effects of five different types of hydrocolloids, including neutral polysaccharide guar gum (0.01%-0.1%), locust bean gum (0.01%-0.1%), protein polysaccharide bovine bone gelatin (0.1%-1%), ionic polysaccharide carrageenan (0.01%-0.1%), and xanthan gum (0.001%-0.01%) on water-holding capacity, textural and rheological properties of soymilk yogurt were studied. The results showed that adding gelatin (≥0.5%) into soymilk yoghurt significantly increased its water holding capacity to 100%. Adding neutral polysaccharides reduced the water holding capacity of soymilk yogurt. No significant effect on water holding capacity was caused by ionic polysaccharide carrageenan. In terms of texture, adding gelatin (1%) and carrageenan (≥0.05%) significantly increased the firmness and adhesiveness of soymilk yogurt. Carrageenan at low concentration and xanthan at high concentration significantly decreased the elasticity of the yogurt. 1% gelatin also significantly increased the cohesiveness of the samples. Neutral polysaccharide guar gum had little effects on the texture of soymilk yogurt. The rheological results showed that 1% gelatin could enhance the viscoelasticity, yield stress, consistency, and pseudoplasticity of soymilk yogurt. Carrageenan at high concentration could significantly increase the viscoelasticity of soymilk yogurt. Furthermore, all types of hydrocolloids used in this study could increase the apparent viscosity of soymilk yogurt significantly.
  • GUO Shuai, HAN Zhihao, HUANG Tian, ZHENG Yan, WANG Yuejiao, BAI Mei, WANG Jicheng, SUN Tiansong, ZHANG Heping
    Food and Fermentation Industries. 2019, 45(3): 7-13. https://doi.org/10.13995/j.cnki.11-1802/ts.018265
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    Soymilk fermented by compounded Streptococcus thermophilus S10 and probiotics Lactobacillus plantarum P-8 (S10+P-8) was compared against commercial starter culture A and B. Their viable bacterial counts, acidity, viscosity, water holding capacity, proportion of active soybean isoflavones, taste characteristics, texture parameters, and sensory evaluation during fermentation at 42 ℃ and storing at 4 ℃ were investigated. The results showed that time of reaching the fermentation end point, acidity, viscosity, and water holding capacity of these three fermented soymilk samples were not significantly different when they reached their fermentation end points. After fermentation, the active isoflavone content changed significantly (P<0.05) in the experimental group and commercial starter culture A. The number of probiotic L. plantarum P-8 in the experimental group was higher than 1.0×109 CFU/g from the completion of fermentation to the end of storage. At the end of storage, the pH of all three samples were about 4.30, and their titration acidity were lower than 74.00 °T. The relative intensity analysis and variation analysis of six basic tastes and three aftertastes indicated that bitterness, astringency, saltiness, and sweetness of these three samples were significantly different (P<0.05). During storage, the sensory score of S10+P-8 group (93.7 on day 28) was distinctly higher than those of the control groups (P<0.05) . Therefore, S10+P-8 as a starter has a great potential in developing fermented soymilk products.
  • LU Jiayi, LI Biansheng, RUAN Zheng
    Food and Fermentation Industries. 2019, 45(3): 14-20. https://doi.org/10.13995/j.cnki.11-1802/ts.018508
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    Strains of lactic acid bacteria are one of the most important factors that impact the qualities of fermented juices. Autochthonous Lactobacillus plantarum and allochthonous L. plantarum LP-115 were used alone or together to ferment pear juices. The volatile aroma compounds of the juices before and after fermentation were measured by gas chromatography-mass spectrometry (GC-MS) after preparing the samples by static-headspace solid phase microextraction (HS-SPME). Changes in free amino acids, polyphenolic compounds, the growth and acidification of bacteria in the juices were also detected. Both autochthonous and allochthonous L. plantarum could metabolize well in pear juices, but autochthonous L. plantarum performed better with a higher final viable count (10.0 lg CFU/mL) and lower pH (pH=3.30). It also had better preservative effects on phenolics and free amino acids. Therefore, it could adapt better to pear juices for fermentation. Lactic acid bacteria fermentation had significant effects on the flavors of pear juices. The allochthonous strain tended to oxidize aldehydes into acids, while the autochthonous strain tended to reduce aldehydes into alcohols. These two strains had synergistic effects on fermentation, which was superior to single bacteria fermentation in terms of proliferation and metabolism of acid production, enriching volatile flavor substances, and preserving free amino acids and phenols.
  • NI Caixin, JIN Xing, ZHOU Wei, CHEN Xiaohua, YIN Boxing, FANG Dongsheng, WANG Gang, ZHAO Jianxin, ZHANG Hao, CHEN Wei
    Food and Fermentation Industries. 2019, 45(3): 21-27. https://doi.org/10.13995/j.cnki.11-1802/ts.018228
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    The scavenging effects of twenty-four strains of Lactobacillus on hydroxyl radicals, DPPH free radicals, and their reducing activities were investigated to compare their antioxidative activities in vitro. Then, Caenorhabditis elegans fed with these twenty-four Lactobacillus strains were put into a 0.003%(mass froution) hydrogen peroxide solution that induced oxidative stress. By comparing the lifespan of C. elegans fed with different Lactobacillus strains, abilities of Lactobacilli to mitigate oxidative damages in C. elegans were analyzed. The results showed that L. plantarum Lp11 not only exhibited high antioxidant capacity in vitro, but also effectively alleviated the oxidative stress in C. elegans with the best effect. The correlation analysis between antioxidant indexes in vitro and LT50 of C. elegans showed that hydroxyl radical scavenging capacity had a strong correlation with oxidative stress alleviation in C. elegans (P<0.01). Therefore, hydroxyl radical scavenging capacity of Lactobacilli can be used to reflect the antioxidant activity of Lactobacilli in C. elegans.
  • QIN Xiuyuan, LIU Wenying, CHEN Licheng, LIU Qi, LU Jun, GU Ruizeng, CAI Muyi, ZHANG Chunle
    Food and Fermentation Industries. 2019, 45(3): 28-33. https://doi.org/10.13995/j.cnki.11-1802/ts.015777
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    Pea oligopeptides and sodium selenite were used as raw materials, the best process for preparing selenium-chelating pea oligopeptides was studied by using orthogonal methods. The results showed that when chelating at 80 ℃ for 30 min, with 5% pea oligopeptides and an oligopeptide to salt mass ratio 2∶1 and at pH=9, the yield and the chelating rate were 27.87% and 57.23%, respectively. The structural characteristics of pea oligopeptides were analyzed by ultraviolet full wavelength scanning (UV), scanning electron microscopy, and infrared radiation (IR). The results showed that the structure of the chelating substances changed before and after chelation, and the optical absorption properties were enhanced. The coordination bonds between selenium ions and NH2+ and —COO— in oligopeptides were formed.
  • LI Yang, LIU Baohua, MA Chunfang, NIU Xiangchen, WANG Zhongjiang, JIANG Lianzhou, LIU Jun
    Food and Fermentation Industries. 2019, 45(3): 34-39. https://doi.org/10.13995/j.cnki.11-1802/ts.017804
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    Rehydration is an important characteristic of freeze-dried foods, to clarify the rule of moisture distribution of rehydrated freeze-dried tofu, effects of ultrasound power (0, 100, 200, 300, 400, 500 W) on moisture distribution and microstructure of fresh tofu and rehydrated freeze-dried tofu were studied by low field nuclear magnetic resonance (NMR). The results showed that the water holding capacity of freeze-dried tofu was significantly lower than that of fresh tofu, which may be due to the changes in the size of the hole in the microstructure of rehydrated freeze-dried tofu, and therefore reduced the retention of water molecules. The results of water distribution showed that suitable ultrasound treatment could provide fresh tofu and rehydrated freeze-dried tofu more structures for intercepting free water components, decreased the T21 relaxation time and increased the M21 peak area percentage. The results of scanning electron microscope showed that ultrasound pulping and freeze drying process could significantly influence the microstructures of these two kinds of tofu.
  • WANG Xin, JIN Peng, Song Peng, Dong Zixing, LIU Xiaoguang, WANG Zhengxiang
    Food and Fermentation Industries. 2019, 45(3): 40-46. https://doi.org/10.13995/j.cnki.11-1802/ts.018387
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    Acid protease has wide applications in foods, brewing, feeds, and leather industries. However, current acid proteases are unstable when the temperature or pH values are higher than 50 ℃ or 3.0, respectively, which restricts their applications. Thus, acid protease encoding gene expA from Aspergillus niger CICIM F0510 was successfully cloned and expressed in Pichia pastoris, and the recombinant strain GS115 (pPIC-expA) was generated in this study. In shaking flask experiments, the activity of recombinant enzyme EXPA was 257 380 RFU/h. Bioinformatics analysis showed that this enzyme belonged to family A1A of aspartic proteases. The optimal temperature and pH of EXPA were 50 ℃ and 3.0, respectively. After incubating at 40-50 ℃ or pH 2.5-3.5 for 1 h, the recombinant enzyme still retained more than 80% of its maximum activity. Zn2+, Ca2+, Fe2+, and Mn2+ enhanced EXPA activity, whereas Cu2+, Co2+, Fe3+, EDTA, and SDS had significant inhibitory effects on EXPA activity. Besides, EXPA also hydrolyzed soybean protein isolates, water-soluble corn protein, and wheat protein. Good thermostability and pH stability of EXPA provide a solid foundation for its applications in food and feed industries.
  • WANG Xuhui, XU Xin, BAO Zhe, WANG Hui, YE Kai, LI Guan, DENG Yu
    Food and Fermentation Industries. 2019, 45(3): 47-55. https://doi.org/10.13995/j.cnki.11-1802/ts.018491
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    Effects of different proportions of microbial agents on the fermentation of cow dung-corn straw combined with biogas fermentation were compared, the high-throughput sequencing technology was used to analyze the microbial diversity in the biogas fermentation broth with the best bacteria. The results showed that the optimal condition to ferment corn straw and cow dung was as follows: microbial agents were Bacillus cereus, Pseudomonas formosensis, Bacillus subtilis, Pseudomonas songnenensis (mass ratio 3∶2∶3∶2). Under these conditions, the highest biogas and methane yield reached 72 mL/g VS and 39 mL/g VS while the highest cumulative biogas and methane yield reached 778 mL/g VS and 532 mL/g VS, respectively. The high-throughput sequencing results showed that Bacteroidetes were the most abundant bacteria and was the most important group of the dominant bacterial species. The relative abundance trend in the biogas fermentation process increased from 33.8% during the early stage of fermentation to 43.6% at the end stage of fermentation. Ascomycota was the most abundant among the fungi. The main dominant group in the biogas fermentation process first increased and then decreased, went from 65.7% during the initial fermentation stages to 84.7% in the middle of the fermentation process and dropped to 66.8% at the end of the fermentation process. The research on combined fermentation of cow dung and corn straw provides a theoretical basis for preparing a biogas agent.
  • WANG Ting, HAN Chao, MAO Qian, ZHANG Dezhi, CAI Ningyun, LIU Hongliang, LI Yanjun, CHEN Ning
    Food and Fermentation Industries. 2019, 45(3): 56-63. https://doi.org/10.13995/j.cnki.11-1802/ts.018611
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    L-2-aminobutyrate is a critical chiral precursor of new drugs, which has been widely used in chemical and pharmaceutical industries. In this study, the metabolic pathway of L-threonine in Escherichia coli THRD was extended to obtain an L-2-aminobutyrate producing strain. The threonine dehydratase encoding genes ilvA2 and ilvA4 were firstly individually overexpressed in E. coli THRD. The resulting strain THRD/pTrc99a-ilvA2 produced 18 g/L L-2-ketobutyrate in a 5 L fermenter by fed-batch fermentation. Subsequently, encoding genes of tyrosine aminotransferase, glutamate dehydrogenase, and leucine dehydrogenase, tyrB, gdh, and bcdBS, respectively, were overexpressed together with ilvA2 to catalyze the conversion of L-2-ketobutyrate to L-2-aminobutyrate. The strain THRD/pTrc99a-bcdBS-ilvA2 produced 19 g/L L-2-aminobutyrate. The effects of disrupting L-threonine exporters on L-2-aminobutyrate fermentation were investigated, and the production of L-2-aminobutyrate in strain THRDΔrhtC/pTrc99a-bcdBS-ilvA2 increased to 22 g/L. Taken together, the results clearly indicated that the L-threonine producing strain could be effectively transformed into an L-2-aminobutyrate producer by extending its downstream metabolic pathway. This study lays a solid basis for constructing L-2-aminobutyrate high producing strains. This study can also be referred in other metabolic engineering studies to biosynthesize new products by pathway extension.
  • ZHOU Jinhu, LI Liang, FANG Shangling, DONG Xiaoyuan, ZHANG Chao, CHEN Maobin
    Food and Fermentation Industries. 2019, 45(3): 64-70. https://doi.org/10.13995/j.cnki.11-1802/ts.018526
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    The experiment was carried out using dilution coating method to coat the mud of the Yellow Crane Tower Luzhou-flavor liquor. Plate scribing purification, microscopic examination, and single-bacteria fermentation were conducted, followed by using gas chromatography-mass spectrometer (GC-MS) to screen functional bacteria with high yield of guaiacol. The results of molecular biology techniques showed that two functional strains, B-1 and B-3, with high-yield of guaiacol were Bacillus amyloliquefaciens and Delfitia sp. X-a12, respectively. Their total yields were 3 632.33 μg/L and 14 527.31 μg/L, respectively. The tolerance analysis results showed that both strains were resistant to 4% vol. alcohol, pH=4.5, and 15% sugar.
  • WU Jiajia, WANG Siqi, DAI Zhiyuan
    Food and Fermentation Industries. 2019, 45(3): 71-76. https://doi.org/10.13995/j.cnki.11-1802/ts.018141
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    Accumulation of biogenic amines in fermented seafood products caused by bacterial activity is a serious food safety problem. This study used selective plates to screen histamine-producing bacteria from traditional salted Chinese herring that were collected from different fermentation periods during its production. The isolates were identified by 16S rDNA sequencing. Histidine decarboxylase genes were amplified using polymerase chain reaction (PCR), while the histamine content in the culture supernatant was detected using high performance liquid chromatography (HPLC). The results indicated that thirty-two presumptive histamine-producing isolates were obtained, which were classified into three genera and eight species. Staphylococcus was the predominant genus (twenty-nine strains), with S. saprophyticus was the dominant species (sixteen strains). Histidine decarboxylase genes were detected among twenty-four isolates, and different concentrations of histamine were detected in the cultures of twenty-three isolates. The average content of histamine produced was 38.10 μg/mL. Among which, one S. saprophyticus strain produced the highest histamine of 100.21 μg/mL. Studying histamine-producing bacteria in salted Chinese herring during its production has theoretical significances in evaluating the safety of salted Chinese herring as well as guiding the selection of fermentation strains scientifically.
  • B1WU Chenshuo, LI Xiaoyue, QIN Mingzhen, YAN Fen
    Food and Fermentation Industries. 2019, 45(3): 77-82. https://doi.org/10.13995/j.cnki.11-1802/ts.018288
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    The recombinant alginate lyase B1SM was isolated and purified, and its enzymatic properties were characterized. The alginate lyase gene B1SM was amplified from the genome of Pseudomonas aeruginosa B1 by Touch down polymerase chain reaction (PCR) and Thermal Asymmetric Interlaced PCR (TAIL-PCR). The gene B1SM was inserted into a pGEX-4T-1 expression vector and expressed in Escherichia coli BL21 (DE3). The results showed that the optimal pH and temperature for B1SM were 8.0 and 25℃, respectively. Moreover, B1SM maintained more than 60% of its relative enzyme activity after incubating at 25℃ for 1 h at pH=7.0-9.0, indicating that it had a good pH stability. Furthermore, it maintained its relative enzyme activity to more than 60% after incubating at 30 ℃ and at pH=8.0 for 1 h. It was concluded that heterologous expression of alginate lyase gene B1SM was achievable in E. coli BL21 (DE3), which laid a foundation for preparing and further study of alginate lyase.
  • NIU Chengtuo, LI Zhengxue, FAN Linxu, QU Guanyi, WANG Songtao, LI Ling, LI Kai, ZHANG Rui, ZHENG Pengfei, LI Qi
    Food and Fermentation Industries. 2019, 45(3): 83-88. https://doi.org/10.13995/j.cnki.11-1802/ts.018174
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    A mulberry fruit wine with good flavors was obtained through yeast screening and optimized fermentation in previous studies. To further elaborate the dynamic changes in bioactive compounds as well as their antioxidant abilities during mulberry wine fermentation, based on the characteristics of mulberry, the pH differential method was optimized to measure the content of anthocyanin. Two anthocyanin monomers in mulberry wine were determined as coumarin-3-glucoside and coumarin-3-rutin using liquid chromatography-mass spectrometry (LC-MS). Furthermore, the contents of bioactive compounds and DPPH scavenging capacity were much higher in this fermented mulberry wine in comparison to commercial grape and mulberry wines. Finally, dynamic changes in the contents of bioactive compounds and anti-oxidant ability of the wine during fermentation and storage period were analyzed. The results showed that both bioactive compounds and DPPH scavenging capacity decreased during fermentation process while both of them maintained stable during storage.
  • WANG Yanli, MENG Yajing, HE Hongkui, ZHANG Huimin, LIU Guoying, WANG Lu, LIU Lu, LI Jingxin
    Food and Fermentation Industries. 2019, 45(3): 89-94. https://doi.org/10.13995/j.cnki.11-1802/ts.018089
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    To reveal the effects of various physicochemical factors on pit mud bacteria and their correlations, differences in physicochemical factors and total bacteria count among degraded, normal, and high-quality Nongxiang flavored pit mud were investigated. The results revealed that the contents of moisture, ammonium nitrogen, calcium, and iron in these three pit mud exhibited significant differences (P<0.05). The contents of calcium and iron in degraded pit mud were 1.90-6.65 and 6.56-24.95 times higher than that in normal and high-quality pit mud, respectively, while other physicochemical indicators showed opposite results. There were no significant differences in pH, humus, available phosphorus and potassium, magnesium, and total bacteria count between degraded and normal pit mud (P>0.05). Compared with high-quality pit mud, there were marked differences in normal pit mud (P<0.05) except magnesium. Principle component analysis (PCA) showed that physicochemical factors in pit mud had clear correlations with the quality of pit mud. The relevance results indicated that the total bacteria count in the pit mud was positively correlated with moisture, pH, ammonium nitrogen, available phosphorus, humus, and available potassium, while it was significantly negatively correlated with the contents of magnesium, calcium, and iron.
  • SHENG Ran, SUN Zhigao, ZHANG Zhen, FANG Ming, YU Fengsheng, ZHANG Qunlin, LI Tian
    Food and Fermentation Industries. 2019, 45(3): 95-102. https://doi.org/10.13995/j.cnki.11-1802/ts.018282
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    The crude carotenoid extracts from Acinetobacter lwoffii UL fermentation were purified by thin layer chromatography (TLC) and silica gel column chromatography to obtain component 1 and component 2. Solubility test, high performance liquid chromatography (HPLC), fourier transform infrared spectrometer (FTIR), 1 hydrogen-nuclear magnetic resonance (1H-NMR), and UPLC-Q-TOF-MS-MS were used to identify component 2. The results showed that pigments in component 2 were fat soluble and had carotenoid characteristic structure, such as methyl , methylene, polyene long chain, benzene ring, and carbonyl group. It was preliminarily deduced that component 2 contained astacen (C40H48O4, molecular weight 592) and few astaxanthin (C40H52O4, molecular weight 596). ABTS, FRAP, and DPPH were used to evaluate the antioxidant activities of carotenoid crude extracts, purified component 1 and component 2. The results showed that carotenoid crude extracts had antioxidant activities, and the antioxidant activities of purified carotenoids increased significantly.
  • TANG Yu, ZHANG Xiaoli, HE Xiaoqin, LI Weizhou, LI Fuhua, MING Jian
    Food and Fermentation Industries. 2019, 45(3): 103-111. https://doi.org/10.13995/j.cnki.11-1802/ts.017675
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    In this study, the Tartary buckwheat bran was pretreated by steam explosion, together with stimulated gastrointestinal digestion in vitro. The amounts of polyphenols released from Tartary buckwheat bran before and after steam explosion and their antioxidant and anti-proliferative activities at different gastrointestinal digestion stages were investigated. The results showed that the amounts of polyphenols released before and after steam explosion as well as their absorption capacity of oxygen free radicals both improved. The cellular antioxidant activity of steam exploded buckwheat bran enhanced during in vitro gastrointestinal digestion. The EC50 values of gastric digestion group and intestinal digestion group were 19.95±0.37 mg/mL and 150.32±6.43 mg/mL, respectively. The gastric digestive fluid of the Tartary buckwheat bran after steam explosion treatment had inhibitory effect on HepG2 cells (EC50=9.37±1.20 mg/mL). This indicated that Tartary buckwheat bran pretreated by steam explosion had certain antioxidant and anti-proliferative activities. This study provides a theoretical basis for scientific evaluation of Tartary buckwheat bran's nutritional value.
  • WANG Yuyan, LIU Haibo, YANG Rong, CHEN Jing, LIU Xiong
    Food and Fermentation Industries. 2019, 45(3): 112-118. https://doi.org/10.13995/j.cnki.11-1802/ts.017659
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    In present study, seven kinds of starch were used to mix with gluten to form reconstituted flour. The rheological properties and microstructures of dough samples with reconstituted flour were determined. This study aimed to investigate whether the starch type is a main factor affects the characteristics of reconstituted dough. The results showed that starch types significantly affected the rheological properties and microstructures of reconstituted dough. 0.05% was selected as the constant strain value of the dynamic frequency sweep. The relationships between storage modulus, loss modulus, and angular frequency of dough samples were well-fitted to the Power-law equation. The elasticity of dough with reconstituted rice flour was the highest, followed by sweet potato, potato, pea, wheat, maize, and highland barley dough. The trend of stickiness was similar to that of elasticity, only the top three orders changed (sweet potato>potato>rice dough). Highland barley and potato dough showed better liquidity than sweet potato, pea, wheat, maize, and rice dough. The creep-recovery tests of each dough indicated that highland barley dough deformed the most, followed by potato, wheat, maize, sweet potato, and pea dough. The rice dough exhibited the smallest deformation. In terms of microstructure, the order of starch binding level in each dough network was: wheat, barley>maize, sweet potato>pea, potato and rice starch.
  • LI Changle, WU Yaqin, WANG Lisha, ZOU Mi, TANG Jiahui, LI Tianxiang, BAO Hairong
    Food and Fermentation Industries. 2019, 45(3): 119-123. https://doi.org/10.13995/j.cnki.11-1802/ts.017678
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    This study aimed to investigate the effects of single ultrasonic treatment and ultrasonic combined acid (glucono-delta-lactone, GDL) treatment on gelling properties of skipjack myofibrillar protein (MP), to improve the added value of skipjack and the quality of skipjack products. MP was used as raw material and either ultrasonic for 3 min or ultrasonic for 3 min combined with GDL, to observe the changes of gelling characteristics of MP. The results showed that ultrasonic for 3 min improved the gel preparation stage of MP, but weakened the gel enhancement stage. In addition, the hardness, elasticity, cohesion, and chewing properties of MP gel decreased obviously. Ultrasonic for 3 min combined with GDL treatment enhanced the MP gel preparation stage and maintained the strengthening ability of original gel. No obvious differences observed between samples treated by ultrasonic for 3 min combined with GDL and original samples in terms of hardness and elasticity, but the coherency and chewiness of treated samples greatly increased. After ultrasonic combined with GDL treatment, the water retention ability of samples increased obviously compared with samples treated by single ultrasonic, but it was lower than that of the untreated group. The solubility of samples treated by ultrasonic and GDL increased obviously compared with original ones, and there was no significant difference in comparison to samples after single ultrasonic treatment.
  • XU Yihan, CHEN Yujing, ZHANG Jianhua
    Food and Fermentation Industries. 2019, 45(3): 124-130. https://doi.org/10.13995/j.cnki.11-1802/ts.018456
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    There are some quality problems after adding potato flour to staple foods, including excessive adhesion and difficulty in molding. Lactic acid bacteria fermentation may improve the texture of staple foods made from potatoes. In this study, Lactobacillus plantarum CGMCC 14177 was used to ferment potato flour in order to improve the texture quality of potato-wheat noodles. By determining the broken rate, cooking loss rate, turbidity, textural property indexes, and sensory characteristics, the effects of fermentation time and ratio of potato flour added on the quality of potato-wheat noodles were investigated. The results indicated that the optimal fermentation period was 10 h, and the maximum addition ratio of fermented potato flour was 30%. Compared with noodles made without adding fermented potato flour, the viscosity of noodles made with fermented potato flour significantly reduced, and the maximal shearing hardness value increased obviously. Besides, their organoleptic properties were similar to wheat flour noodles.
  • GAO Qi, XU Yanling, ZHAO Mengge, ZHU Biyang, HE Hui
    Food and Fermentation Industries. 2019, 45(3): 131-137. https://doi.org/10.13995/j.cnki.11-1802/ts.018313
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    In order to utilize salted duck egg white protein, which is a by-product in food industries, the duck egg white protein was used to produce high calcium-binding peptides by hydrochloric acid deamidization and enzymolysis. The results showed that the degree of deamidization was significantly affected by temperature and hydrochloric acid concentration (P<0.05). The deamidization degree reached 72.67% under the optimized deamidization conditions. Furthermore, papain was chosen as the optimal enzyme. In terms of calcium binding capacity, effects of enzymolysis were significantly affected by enzymatic hydrolysis time and solid-liquid ratio (P<0.05) and extremely affected by enzyme concentration (P<0.01). Under the optimal enzymatic hydrolysis condition, the binding amount of soluble calcium in the deamidated duck egg white peptide was 14.42 mg per peptide. After ultrafiltration, it was demonstrated that the fraction with smaller molecular weight had stronger calcium binding ability.
  • LI Ping, LI Tong, GAO Yingying, ZHANG Cuiying
    Food and Fermentation Industries. 2019, 45(3): 138-142. https://doi.org/10.13995/j.cnki.11-1802/ts.018462
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    This study aimed to explore the effects of inoculating Lactobacillus plantarum during alcoholic fermentation (AF) at different time to generate a co-fermentation of Saccharomyces uvarum and L. plantarum, to balance the effect of AF and malolactic fermentation (MLF) on wine. The results showed that L-malic acid metabolized from 5.04 g/L to 1.30 g/L on day 15 when L. plantarum was inoculated on the first day of AF, leading to increased fermentation rate and considerable reduction in overall fermentation duration. In addition, simultaneous inoculation not only reduced acidity and higher alcohol contents, but also improved the production of esters, especially when the ethyl lactate content increased to 24.313 mg/L, which improved the flavor quality of the wine.
  • SU Hao, HE Zhigang, LI Weixin, REN Xiangyun, LIN Xiaozi, LIN Xiaojie, LIANG Zhangcheng
    Food and Fermentation Industries. 2019, 45(3): 143-147. https://doi.org/10.13995/j.cnki.11-1802/ts.018571
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    This study aimed to explore the effects of free SO2 in wines on oxidative browning of wines and to establish an early and rapid warning strategy regarding the oxidative browning of wines. The browning degree, free SO2 content, as well as the changes in redox potential during oxidative browning of two kinds of southern wild wine were analyzed using accelerated oxidation method. The results showed that free SO2 decreased rapidly, while the browning degree and the redox potential of wines increased rapidly during accelerated oxidation. Free SO2 inhibited oxidative browning of wines, but its antioxidant activity became weaker when its content was lower than 3.92 mg/L. There was a significant negative correlation between free SO2 and redox potential of wines. The redox potential can be measured in real time to monitor free SO2 content and to provide a rapid warning of oxidative browning of wines.
  • LIU Shan, YANG Liu, HE Shudong, SUN Hanju, YAO Shengfei, XU Shangying
    Food and Fermentation Industries. 2019, 45(3): 148-152. https://doi.org/10.13995/j.cnki.11-1802/ts.016581
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    In order to improve the product quality and utilization value of rice wines lees, producing cooking wine using protease hydrolysates of rice wine lees was investigated. The effects of pretreatment methods on dissolution rate of yeast protein, effects of protease kinds on enzymatic hydrolysis of lees protein and also effects of the addition form and amount of enzyme hydrolysates on the fermentation of wine were explored. The results showed that using boiling water bath for 5 min was beneficial for dissoluting yeast protein and the dissolution rate of yeast protein increased by 48.21% compared with that of control. Alkaline protease was selected to hydrolyze rice wine lees. Cooking wine was produced using 2.0 g/L protease hydrolysate mixture of lees as adjuvant together with rice mash. Amino acid nitrogen and free amino acids contents in base liquor were 0.521 g/L and 3.553 g/L, respectively, which were 391.51% and 404.69% higher than those of control, respectively. The amino acid flavor ratio (the sum of umami amino acids and sweet amino acids to the sum of bitter amino acids and astringent amino acids) was 0.690, which was 62.35% higher than that of control
  • LI Rui, ZOU Qian, SUN Yulin, WANG Lin, LI Xiang, FENG Minghui
    Food and Fermentation Industries. 2019, 45(3): 153-160. https://doi.org/10.13995/j.cnki.11-1802/ts.018280
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    The crayfish (Procambarus clarkia) head contains abundant endogenous enzymes. Its protein hydrolysates with umami flavour can be prepared by ultraviolet-induced autolysis. According to the degree of hydrolysis (DH), single-factor experiments and response surface methodology were used to investigate the optimal autolysis conditions. The optimal autolysis conditions were as follows: ultraviolet irradiated for 22.94 min,autolysed with an initial pH of 7.16 at 50.06 ℃ for 3 h. Under this condition, the DH of crayfish head was 44.39%. The umami substances in crayfish head original fluid and autolysed products were analyzed by sensory evaluation and physicochemical indexes. The results showed that the fishy and bitter tastes of autolyzed crayfish head decreased, while the umami flavour and the mellow sense increased significantly (P<0.05). The contents of umami total amino acids (2 150.12 mg/kg) and free amino acids (8 605.45 mg/kg) in autolyzed products increased by 1.41 and 1.29 times, respectively, compared with the original fluid. The contents of nucleotides and its related compounds after autolysis changed slightly (P>0.05). Inosine monophosphate (IMP), which greatly contributes to umami flavor, was 8.36 mg/kg in autolysed products, which was increase by 47.18%. The autolysed hydrolysates of crayfish head had intense delicious prawn flavour and rich tastes, which can be used as a nutritious, healthy, and delicious material for condiments.
  • SHE Wanwan, WEI Yaqing, WANG Yameng, HE Xiaomei, ZHANG Hongcui, JIN Miaomiao, ZHANG Min
    Food and Fermentation Industries. 2019, 45(3): 161-167. https://doi.org/10.13995/j.cnki.11-1802/ts.017715
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    The temperature changing logistics (cold storage, transported at room temperature with insulating packaging for 3 days, stored in household or commercial refrigerator ) of spicy chicken was studied to investigate the effect of intermittent microwave treatment on suppressing the inferior quality of spicy chicken slice. At the end of transportation at normal temperature (day 5), the spicy chicken was left untreated or treated by intermittent microwave (microwave for 30 s, intermittent cooling for 60 s, re-microwave for 30 s) and continuous microwave (microwave for 60 s). The results showed that two kinds of microwave treatments effectively avoided water loss from spicy chicken, and delayed increases in pH, total volatile basic nitrogen (TVB-N) value, and total number of colonies during shelf life. The intermittent microwave treatment prolonged the logistics period to 35 days, and the flavor, color, and texture of the tissue maintained better with the best preservative effects. However, the intermittent microwave treatment had no obvious inhibitory effect on fat oxidation. In summary, intermittent microwave treatment has better effects on controlling quality deterioration of spicy chicken during temperature changing logistics.
  • ZHANG Dongya, HU Bokai, Li Weian, XU Li
    Food and Fermentation Industries. 2019, 45(3): 168-175. https://doi.org/10.13995/j.cnki.11-1802/ts.017661
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    In order to obtain Zao-peppers with better tastes and flavour, this experiment improved the formulation and technology used for producing traditional Zao-peppers. This experiment measured volatile compounds, organic acids, and physicochemical properties of two kinds of Zao-peppers. The results showed that in comparison to traditional Zao-peppers, the contents of reducing sugar, amino acid nitrogen, and total organic acids in flavored Zao-peppers increased by 2.978 0 g/kg, 0.03 g/kg, and 1.241 7 g/kg, respectively. The contents of lactic acid, malic acid, and citric acid in flavored Zao-peppers increased, which modified the stimulating, sour, and astringent tastes of traditional Zao-peppers. In other words, the sourness of flavored Zao-peppers was more gentle and refreshing, with an increased taste score. Secondly, more than twelve kinds of flavor compounds were detected in flavored Zao-peppers. The flavor and sensory scores of flavored Zao-peppers increased, as the types and contents of flavor-contributing alcohols and esters increased, which made flavored Zao-peppers more fragrant. The flavored Zao-peppers produced by the new processing formula has higher commodity value. Therefore, this study can be a reference for developing Zao-peppers industries in Yunnan and Guizhou provinces.
  • LIU Jiaxuan, PENG Mengchen, YANG Xuejie, LEI Yang, HUANG Xiaoli, WANG Jun
    Food and Fermentation Industries. 2019, 45(3): 176-182. https://doi.org/10.13995/j.cnki.11-1802/ts.017509
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    Hot air drying experiments on apricot were conducted after radio frequency (RF) pretreatment with different treatment time (20,30,40,50 min). The moisture content, changes in nutritional components of apricot during drying as well as dried apricot were determined. The drying kinetic model of hot air dried apricot was established (Page model, Herdenson and Pabis model, Lemus model). The results showed that RF pretreatment could improve the hot air drying rate of apricot. Herdenson and Pabis models were better fit to the experiment data according to the analysis of R2, χ2, and root-mean-square error (RMSE) of different models. After RF pretreatment, levels of flavonoids, polyphenols, and vitamin C in dried apricot fruit were higher than those of fresh fruit. The nutrient contents of dried apricot were the highest when the RF pretreatment time was 30 min, with the contents of vitamin C, flavonoid, and polyphenols increased by 0.172 6 mg/100g, 4.501 4 mg/100g, and 6.244 9 mg/100g, respectively. RF may be used as a pretreatment to improve the drying rates of fruits and vegetables, and also to help nutrients' dissolution or extraction.
  • WANG Tingting, YUAN Wei, LI Hua, WANG Hua
    Food and Fermentation Industries. 2019, 45(3): 183-188. https://doi.org/10.13995/j.cnki.11-1802/ts.018443
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    The contents of phenolic substances in compounded small berry wines made from different raw materials in four different ratios, as well as their retention rates and antioxidant capacities were studied. Meanwhile, the effects of each phenolic on free radical scavenging capacity of ABTS, DPPH and hydroxyl (·OH) were also analyzed. The results showed that in comparison to compounded wines made from red small berries, total phenol, total flavonoid, total anthocyanin, flavonol and flavanol contents in compounded wines made from black small berries were higher, while total phenolic acid and ellagitannin contents showed opposite results. Total phenolic content showed a strong positive correlation with ABTS free radical scavenging rate (r=0.817). Additionally, total phenolic acid and ellagitannin contents showed positive correlations with DPPH free radical scavenging rate (r=0.950 and r=0.895, respectively) and hydroxyl (·OH) free radical scavenging rate (r=0.769 and r=0.812, respectively). This indicated that black small berry wine had better ABTS free radical scavenging capacity, and compounded small berry wine with red berries had better DPPH and hydroxyl (·OH) radical scavenging capacity.
  • LIU Xiaoyu, LI Ke, WEI Guangxin, ZHANG Weiguang
    Food and Fermentation Industries. 2019, 45(3): 189-195. https://doi.org/10.13995/j.cnki.11-1802/ts.017367
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    Simultaneous distillation extraction (SDE), space-solid phase micro extraction (SPME), and monolithic material sorptive extraction (MMSE) were used to extract volatile compounds from Stauntonia chinensis wine made by pure fermentation. All the extracted flavor compounds were identified by gas chromatography-mass spectrometry (GC-MS) and retention index analysis. The results showed that seventy-five volatile compounds were extracted, including alcohols, esters, acids, carbonyls, aromatics, terpenes, and other compounds. Comparing the characteristics of the extracts extracted by these three methods, SDE performed better in terms of extracting alcohols and acids with high molecular weights and boiling points, while SPME was the best to extract alcohols, esters, and carbonyls with low boiling points. Meanwhile, MMSE could extract alcohols, esters, carbonyls, and aroma aromatics normally, but it was not as efficient as SPME to extract esters. Overall, SPME was the most suitable method to extract flavor compounds from Stauntonia chinensis wine.
  • HUANG Yukun, TIAN Hongmei, CHEN Fang, CHEN Tingting, CHE Zhenming, LIU Ping, DING Wenwu
    Food and Fermentation Industries. 2019, 45(3): 196-205. https://doi.org/10.13995/j.cnki.11-1802/ts.017118
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    To explore the differences of three types of edible tallow flavors (no fragrance, light fragrance, fragrant) in terms of their volatile compounds as well as their formation mechanisms. Volatile compounds and their relative contents in three kinds of tallow were determined by electronic nose (E-nose) and headspace solid-phase microextraction-gas chromatography-mass spctrometry (HS-SPME-GC-MS). Relative odor activity value (ROAV) was used to analyze the key flavor compounds in beef tallow. The results showed that E-nose could distinguish the flavors of different types of tallow sensitively. Moreover, linear discriminant analysis(LDA)showed better results than principle component analysis (PCA). Besides, a total of sixty-two volatile compounds were identified by HS-SPME-GC-MS. Aldehydes were found to contribute to most of the aroma, which mainly contained isovaleraldehyde, hexanal, heptaldehyde, octanal, and 1-nonanal. Both trans,trans-2,4-nonadienal and valeraldehyde acted as flavor enhancers to different levels. Other than aldehydes, both dextroterpenes and 2-n-pentylfuran also contributed to different aroma of the tallow. (-)-limonene and 2-methylnaphthalene were important sources of characteristic flavor of fragrant butter, and ester compounds probably had special effects on enhancing the flavor. In conclusion, main flavor compounds and their relative contents showed significant differences in three types of tallow. The mechanisms of forming different flavors of tallow were preliminarily inferred as aldehydes, which were the main ingredients caused changes in key aromas.
  • FAN Zhenyu, ZHANG Long, YUAN Kai, WANG Xichang, LIN Yujin, LIU Yuanping
    Food and Fermentation Industries. 2019, 45(3): 206-214. https://doi.org/10.13995/j.cnki.11-1802/ts.017698
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    This study aimed to explore the effect of pH shift on odor characteristics of fish protein. Scomberomorus niphonius was used as the research object in raw and cooked states. The proximate composition and amino acid composition of raw meat, water washing surimi (WM), acid-aided protein isolates samples(AC), and alkali-aided protein isolates samples(AK) were compared. The odor characteristics of AC and AK were focused. Electronic nose (E-nose) was used to determine the differences in odor profiles of the four samples. The odor substances were further quantified by solid phase microextraction-gas chromatography mass spectrometry (SPME-GC-MS). The results showed that E-nose could effectively distinguish the odor profiles of the four cooked samples. Compared to raw meat, the differences in odor profiles from the largest to the smallest: WM>AC>AK. Compared with cooked samples, raw samples had the same variability but less discrimination. Further quantitative analysis showed that twenty-six, nineteen, twenty-three, and twenty-two volatile compounds were detected in four raw samples. Twenty-three, twenty-six, thirty-two, and twenty-four volatile compounds were detected in four cooked samples. Aldehydes, ketones, and alcohols were the main volatile compounds in all samples. Compared with raw meat, the kinds and contents of alcohols and ketones in other processed samples changed, and there was a big difference between cooked and raw samples. It was concluded that pH shift could effectively reduce the fishy off-flavor of Scomberomorus niphonius. The fish protein isolates had better odor characteristics than WM. Therefore, protein sources with heavy fishy off-flavor could use this method to make proteins have good odor and have high protein content and low lipid content etc., providing a theoretical basis for its further processing.
  • CHEN Xiaoyu, LU Lixia, XIONG Xiong, LIU Yuanjian, XIONG Xiaohui
    Food and Fermentation Industries. 2019, 45(3): 215-222. https://doi.org/10.13995/j.cnki.11-1802/ts.017614
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    To establish a highly specific, sensitive, and time-saving polymerase chain reaction (PCR) method to identify bovine-derived ingredients, a set of primers were designed, based on conserved sequence of the mitochondrial cytochrome b gene (Cytb) of bovine species. The specificity of the method was evaluated by analyzing three kinds of cattle (yak, buffalo, scalper), and several other common tissues (mammalians, ruminants, avian, vegetation). The results showed that the method was highly specific for bovine-derived ingredients, as non-bovine ingredients were not amplified. The sensitivity of the method was evaluated by using common cattle breeds. The results showed that the minimum amount of bovine DNA that could be detected was 0.2 pg (fresh meat). The beef was mixed with pork, donkey, lamb, and soybean tissues, respectively, for PCR analysis. The results indicated that the lowest detection limits of bovine-derived ingredients in both fresh samples and also in samples that were autoclaved at 121 ℃ under 0.1 Mpa for 20 min were 0.01% mass fraction. The test results of commercial foods tested by this method were completely consistent with current standard methods for detecting bovine-derived ingredients.
  • ZHANG Jing, PAN Shuli, MA Liang, GUO Ting, ZHANG Yuhao
    Food and Fermentation Industries. 2019, 45(3): 223-230. https://doi.org/10.13995/j.cnki.11-1802/ts.018185
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    A novel indirect highly sensitive competitive chemiluminescent immunoassay was established to determine ochratoxin A. Oxidation of 3-(4-hydroxyphenyl) propionic acid urea peroxide was catalyzed by horseradish peroxidase that was on an enzyme-labeled ochratoxin A secondary antibody to generate a fluorescent 3-(4-hydroxyphenyl) propionic acid dimer. A strong chemiluminescence was produced by the reaction between bis (2,4,6-trichlorophenyl) oxalate and urea peroxide in acetonitrile medium under the action of imidazole. The content of ochratoxin A in samples was determined by luminescence intensity. The results showed that under the optimal conditions, the IC50 was 0.55 ng/mL with a good linearity in the range of 0.05-6.08 ng/mL, and a detection limit of 0.01 ng/mL. The average recovery rates of raisins and grape juice samples were 84.55%-91.36% and 73.32%-87.64%, respectively. The intra-assay and inter-assay variation coefficients were both less than 10% with good precision. This novel chemiluminescence method has higher luminescence intensity and longer emission time when detecting ochratoxin A. It can be used for detecting trace ochratoxin A in foods with high-sensitivity.
  • CHEN Jinghui, SHI Hao, ZHANG Qiang, MA Yiyu, WANG Hongxin
    Food and Fermentation Industries. 2019, 45(3): 231-236. https://doi.org/10.13995/j.cnki.11-1802/ts.017488
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    Electronic nose (E-nose) and headspace-solid phase micro extraction-gas chromatography mass spectrometry (HS-SPME-GC-MS) were used together to evaluate and compare volatile compounds of Cymbopogon Citratus extracted by different methods. The ability of E-nose to distinguish volatile components of Cymbopogon Citratus was also evaluated. The results of both E-nose and HS-SPME-GC-MS showed that volatile compounds of Cymbopogon Citratus extracted by water were similar to its essential oil, especially from boiling water. In addition, twenty-four kinds of volatile compounds were analyzed by HS-SPME-GC-MS,such as neroli, geranyl, and geraniol etc. Therefore, Cymbopogon Citratus brewed with boiling water can obtain most of its volatile compounds. E-nose combined with HS-SPME-GC-MS can be used as an effective technique to analyse and discriminate volatile compounds of Cymbopogon citratus, and also for its quality control during process.
  • LI Dalei, WENG Yanru, DU Liping, WANG Chao, MA Lijuan, XIAO Dongguang
    Food and Fermentation Industries. 2019, 45(3): 237-245. https://doi.org/10.13995/j.cnki.11-1802/ts.017410
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    In order to understand the differences in aroma components of different brands of Pu-erh tea, volatile compounds and characteristic aroma of three kinds of Pu-erh tea were analyzed and identified by electronic nose (E-nose) and headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that E-nose could distinguish three brands of products well. The principal component analysis showed that the differences between different brands were obvious with good distinction. Further HS-SPME-GC-MS analysis showed that seventy-four compounds in total were identified from three brands of Pu-erh tea. Among them, thirty-eight compounds were shared between all brands of Pu-erh tea tested. Sixty-six, fifty-three, and forty-eight volatile compounds were identified in Dayi Pu-erh tea, Lao Tongzhi Pu-erh tea, and Lancang ancient tea, respectively. The main compounds were aldehydes, alcohols, ketones, and methoxy-phenolic compounds etc. Differences in varieties and contents of volatile compounds between different brands of Pu-erh tea were significant. The main compounds that showed differences including 2-hydroxy-6-methoxy-benzaldehyde, safranal,linalool oxide, terpinen-4-ol, 6-methyl-5-hepten-2-one, (E,E)-3,5-octadien-2-one, 1,4-dimethoxy-benzene, 1-methoxy-4-(1-propenyl)-benzene, 1-methyl-2-(1-methylethyl)-benzene, α-terpinene, dimethyl phthalate, and caffeine. Analysis of odour active values showed that different odors were caused by (E)-2-octenal, (E,E)-2,4-nonadienal, nonanal, α-ionone, and geranyl acetone.
  • CHANG Rui, MA Mengjun, LUO Liyong, JIANG Rui, ZHANG Li, ZENG Liang
    Food and Fermentation Industries. 2019, 45(3): 246-253. https://doi.org/10.13995/j.cnki.11-1802/ts.017381
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    This study aimed to investigate the variations in quality components and to screen the characteristic components of Hubei Qingzhuan brick tea with different storage years. Fifteen samples of Qingzhuan brick tea were detected by high performance liquid chromatography and automatic amino acid analyzer. The experimental data was analyzed by multivariate statistical analysis and Heatmap for screening and clustering. The results showed that the contents of flavonoids and flavonols (myricetin, quercetin, kaempferol), theabrownin, gallic acid, salicylic acid, and succinic acid increased with storage time. In comparison, polyphenols, catechins (epicatechin and epigallocatechin gallate), free amino acid monomers (theanine, alanine, cysteine, leucine, phenylalanine, lysine, and histamine acid) decreased with storage time. The principal component analysis screened ten kinds of characteristic components, such as flavonoids, myricetin, salicylic acid, theabrownin, theanine, and tea polyphenols etc. The results of cluster analysis and Heatmap showed that the quality of Hubei Qingzhuan brick tea began to change after storing for 7 years, and the differences were significant after storing for 9 years. The key quality components affected clustering by year were tea polyphenols, theaflavin, and flavonoids.
  • YANG Yini, YANG Guiqiu, YANG Xiaohong, ZHENG Fuping, YU Ainong
    Food and Fermentation Industries. 2019, 45(3): 254-260. https://doi.org/10.13995/j.cnki.11-1802/ts.017579
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    This study aimed to determine the volatile compounds of Wuling area Hawk tea by extracting volatile compounds by headspace solid-phase microextraction and needle trap. The results indicated that forty-seven volatile compounds were identified in Hawk tea, which included thirty-two kinds of free volatile compounds and twenty-six kinds of bound volatile compounds. The results showed that the main free volatile compounds were bornyl acetate, cadinol, and α-eudesmol. Bound volatile compounds were γ-eudesmol, α-selinene, and guaiene. There were seventeen free and bound volatile compounds with odor activity value (OAV) greater than 1. Based on the odor characteristics of those identified compounds, it was concluded that free odor of Hawk tea was mainly from decanal, dodecanal, bornyl acetate, spathulenol, undecan-2-one, and hexanal. Bound odor were β-damascenone, spathulenol, linalool, 2-hexanone, D-limonene, undecan-2-one, and decanal.
  • ZHANG Jiukui, JIA Nan, KE Runhui, WANG Shuo, LI Xiaobin, YIN Jianjun
    Food and Fermentation Industries. 2019, 45(3): 261-265. https://doi.org/10.13995/j.cnki.11-1802/ts.015641
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    A new solid phase extraction coupled with high performance liquid chromatography method for quantitatively analyzing two fatty acid tryptamides (FATs), behenic acid tryptamide and lignocerinic acid tryptamide, was established. The cocoa shell content in cocoa powder was calculated according to the differences in FATs contents in the powder and shell. The samples were extracted by trichloromethane with vortex and ultrasonic extraction. The Venusil C18 chromatographic column was used and eluted with acetonitrile/tetrahydrofuran/water,followed by using a fluorescence detector. The results showed that these two FATs showed good linear relationship (r>0.999 1) within their linear range(50-1 000 μg/L). The detection and quantification limits were 0.03 mg/kg and 0.1 mg/kg, respectively. The spike recovery rate was 92%-103% and the RSDs was 2.1%-6.2%. In addition, FATs levels and the cocoa shell contents in cocoa powder samples were processed to establish a simple analysis model. The prediction values had good corresponding relationship with true values. The results showed that cocoa powder adulterated with 5% or more (mass fraction) cocoa shell could be identified by this method.
  • ZHANG Hongyan, DING Wu, WU Fuxiang, WANG Xiaoqiao, LIU Xiaoxiao, MIAO Qian, ZHANG Jie
    Food and Fermentation Industries. 2019, 45(3): 266-269. https://doi.org/10.13995/j.cnki.11-1802/ts.017281
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    This study aimed to establish a method that uses inductively coupled plasma mass spectrometry (ICP-MS) to simultaneously determine lead, arsenic, aluminum, chromium, iron, and copper in dried aquatic products. Samples were digested in automatic graphite digestion, followed by quantitative tests using internal standard method by ICP-MS. The results showed that the concentrations of lead, arsenic, aluminum, chromium, iron, and copper showed good linear relationships in the range of 0.25-25 μg/L, with correlation coefficients (R2) higher than 0.990. The detection limits for lead,arsenic,aluminum,chromium,iron,and copper were 0.02 μg/L, 0.08 μg/L, 0.12 μg/L, 0.10 μg/L, 0.03 μg/L, and 0.05 μg/L, respectively. The recovery rate was 84.0%-108.4%. The relative standard deviation of reproducibility was less than 10% (n=6). This method was proved to be simple, accurate, with low detection limits and good reproducibility, which can be used to detect metals in dried aquatic products.
  • BAI Juan, ZHANG Yao, WANG Xuerui, XUE Shuanshuan, WANG Chengming
    Food and Fermentation Industries. 2019, 45(3): 270-274. https://doi.org/10.13995/j.cnki.11-1802/ts.017848
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    The flavor of fermented meat rice during different fermentation periods were determined by E-tongue and E-nose. The data was analyzed by principal components analysis (PCA), discriminate factor analysis (DFA) and radar charts. The results showed that different fermentation time had great impacts on the flavor of fermented meat rice. Both E-tongue and E-nose could be used to analyze the flavor changes in fermented meat rice during different fermentation periods. Moreover, DFA could better distinguish the changes in flavor substances of fermented meat rice than PCA. Furthermore, the radar charts could directly show the variation of flavor substances.
  • JIN Ting, SUN Xin, LI Zhuowa, FENG Xiaohui, TAN Shengbing, WANG Minglin
    Food and Fermentation Industries. 2019, 45(3): 275-280. https://doi.org/10.13995/j.cnki.11-1802/ts.015976
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    QuEChERS and gas chromatography-triple quadrupole mass spectrometry were adopted as an efficient method to detect and analyze nineteen organophosphorus pesticide residues in pomegranates. The sample was prepared by optimized QuEChERS, extracted with ethyl acetate and purified by primary secondary amine sorbent (PSA) adsorbent. The nineteen organophosphorus pesticide residues were separated by an Rtx-5MS column, and determined using MS/MS in multiple reactions monitoring mode. All correlation coefficients of calibration curves were over 0.99 in the linear concentration range 0.01-1.0 mg/L. The detection limit (S/N=3) was 0.9-3.7 μg/kg, and the quantitation limit (S/N=10) was 3-12.2 μg/kg. The recovery rate was 73.5%-120.9% with relative standard deviations (RSDs) between 1.1% and 15.7%. Two kinds of pesticides, monocrotophos and dimethoate, were detected from pomegranates available from the market by this method. This method proved to be rapid, accurate, and with high sensitivity. It can be applied for simultaneous determination of organophosphorus pesticide residues in pomegranates
  • WANG Jinjin, YUAN Haibo, DENG Yuliang, HUA Jinjie, DONG Chunwang, JIANG Yongwen
    Food and Fermentation Industries. 2019, 45(3): 281-287. https://doi.org/10.13995/j.cnki.11-1802/ts.017261
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    Tea production has a strong timeliness and seasonal characteristics. As tea is porous, this makes it very susceptible to the environment during long-term storage. As one of the three most popular drinks in the world, tea is favored by consumers. Therefore, maintaining the quality of tea during storage has an important role in improving the value of tea commodity and promoting the development of tea industries in China. In recent years, various preservative techniques have arisen based on the reasons of quality changes during storage. This research summarized the mechanisms of quality deterioration of green tea, oolong tea, and black tea by discussing oxidative degradation of tea polyphenols, amino acids, vitamin C, chlorophyll, lipid, and other substances. Factors that affect tea quality during storage, such as moisture, temperature, oxidation, and light exposure were also reviewed. The preservative techniques of tea, including drying, refrigeration, deoxygenation, and bio-preservation etc. were discussed in detail. Further research direction in terms of tea preservation was prospected. This study provided scientific guidance for studying new technology for tea storage.
  • YU Zhuoran, HONG Housheng
    Food and Fermentation Industries. 2019, 45(3): 288-292. https://doi.org/10.13995/j.cnki.11-1802/ts.018413
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    Red yeast has a range of applications in wine making, fermented bean curd, vinegar, food coloring, and traditional Chinese medicine. In recent years, functional Monascus with cholesterol-lowering substance Monacolin K as the main component has been increasingly favored by the health care market. How to improve the content of Monacolin K in red yeast fermentation products has become a main technical problem that restricts the development of industries that produce functional red yeast. In this paper, characteristics of Monascus, the structure of Monacolin K and its cholesterol-lowering mechanism and its yield controlling strategy were summarized, aimed to provide references for improving functional red yeast Monacolin K production and promote the development of industries that produce red yeast health care products.