15 January 2021, Volume 47 Issue 1
    

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  • ZENG Weizhu, SHAN Xiaoyu, FANG Jun, ZHOU Jingwen
    Food and Fermentation Industries. 2021, 47(1): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.024789
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    An engineered Escherichia coli constitutively expressing sorbose dehydrogenase (SDH) was previously constructed for producing the vitamin C precursor 2-keto-L-gulonic acid (2-KLG) with L-sorbose as substrate. However, this strain has a poor tolerance to L-sorbose. In order to solve this problem, adaptive laboratory evolution was carried out. Firstly, to enhance the tolerance of L-sorbose, the starting strain was grown and subcultured in the medium with different gradient concentration of L-sorbose by applying Microbial Microdroplet Culture System designed based on microfluidic technology. An evolutionary strain 2-F6 that could tolerate high concentration of L-sorbose was obtained after further verifying on the shaking flasks. Then, the sorbosone dehydrogenase (SNDH) was expressed in 2-F6 to promote the strength of 2-KLG production. The inoculation amount, fermentation temperature, induction time for SNDH, IPTG concentration and L-sorbose addition were optimized in the shaking flasks, and the titer of 2-KLG reached 6.05 g/L under the optimized optimal conditions. Finally, after scaling up in a 5 L fermenter, 2-KLG of 5.70 g/L was obtained. The results obtained could provide references for the production of vitamin C precursor 2-KLG with one-step fermentation process.
  • SUN Xi, LIU Haiqing, ZHANG Jun, FAN Zhihua, HUANG Liang
    Food and Fermentation Industries. 2021, 47(1): 8-13. https://doi.org/10.13995/j.cnki.11-1802/ts.025066
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    Stress tolerance of baker’s yeast (Saccharomyces cerevisiae) is critical to the baking industry. In this study, the high sugar tolerance of cryotolerant yeast B+MAL62 obtained previously was determined and its growth characteristics, morphological stability, intracellular trehalose and glycerol content and gas production under high sugar stress were tested and compared with the commercially high-sugar tolerant yeast. Results showed that compared with the control strain, at 40%-60% sugar stress, the intracellular trehalose and glycerol levels in B+MAL62 strain were increased by 55.03%-64.27% and 1.2-1.3 times, respectively. Moreover, its morphological stability, gas production rate and final gas production in high sugar stress were better than the control strain and the commercial sugar-tolerance yeast. These results showed that overexpression of MAL62 could improve the sugar tolerance of baker's yeast. The study reveals a new way of enhancing multi-tolerance of baker’s yeast and improving the knowledge of how the maltose metabolism affects the multi-tolerance of baker’s yeast. This study provides a valuable insight into breeding of novel multi-resistant baker's yeast strains and a better understanding of other sugar metabolism, which has the potential to promote the technological level of yeast industry.
  • SHI Jiajia, QI Tianyi, ZHANG Meng, CHEN Linxia, ZHANG Di, BAO Zhihua
    Food and Fermentation Industries. 2021, 47(1): 14-20. https://doi.org/10.13995/j.cnki.11-1802/ts.024698
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    The aims of this study was to examine the lactic acid bacteria (LAB) microflora during Jiaosu (naturally fermented product from plant) fermentation and to determine the heavy metal tolerance of LAB isolates. A high-throughput sequencing approach based on 16S rRNA gene amplicons was used to analyze the bacterial community structure, including LAB and pathogenic bacteria, at different fermentation periods in two tailor-made fermenters. The predominant LAB was isolated and their tolerance and absorption to heavy metals such as Pb2+ and Cd2+, was determined. Results showed that the changes in the bacterial community were relatively similar in both samples throughout the fermentation process; Lactobacillus was the predominant LAB at all four fermentation stages (relative abundance, 25.8%-70.9%), with the highest relative abundance at day 20 of fermentation. As to the species, the diversity varied greatly during different fermentation periods. The relative abundance of Lactobacillus pentosus increased from day 10 (22.5%-26.5%) to day 20 (61.6%-65.7%), while the relative abundance of Escherichia coli decreased sharply. A total of 39 LAB strains were isolated, of which L. pentosus and L. brevis were the predominant strains. All three representative strains demonstrated stronger tolerance to Pb2+ than to Cd2+, with a Pb2+ removal rate of 78.47%. in L. pentosus strain AA11. Therefore, Lactobacillus was the dominant bacterial group present in Jiaosu, and L. pentosus not only markedly inhibited E. coli during the early stage of fermentation, but also indicated application potential for removal of Pb2+ in food.
  • YU Jie, XU Qinqian, LI Ziyuan, LIU Hongyan, HAO Zaibin, LI Haiyun
    Food and Fermentation Industries. 2021, 47(1): 21-26. https://doi.org/10.13995/j.cnki.11-1802/ts.025545
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    In order to obtain new microbial strains that can efficiently transform polydatin into resveratrol, four endophytic fungi, numbered as HZ001, HZ002, HZ003 and HZ004 were isolated from Polygonum cuspidatum by selective media using polydatin as the sole carbon source. The activity of four strains of cells and fermentation broth to transform polydatin were identified, and the morphological and molecular biological identification of the strains were carried out. The results of cell transformation assays showed that, polydatin could be transformed into resveratrol by cells of HZ001 and HZ004 with the molar conversion rates of 100%, while no resveratrol was detected in HZ002 and HZ003 cell transformation systems. The results of fermentation broth biotransformation assays showed that polydatin could be transformed into resveratrol by fermentation broths of HZ001, HZ003 and HZ004 with the conversion rates of 55%, 40% and 38%, respectively. The results indicated that HZ001 and HZ004 had the strongest activities to convert polydatin into resveratrol, which mainly depended on the intracellular enzymes. The results of morphological and molecular biological identification indicated that HZ001 and HZ002 were attributed to Aspergillus aculeatus and Penicillium georgiense respectively, both HZ003 and HZ004 were attributed to Aspergillus flavus.
  • WU Xiaohong, GAO Shengping, JIANG Caiyun, WANG Qingzheng
    Food and Fermentation Industries. 2021, 47(1): 27-34. https://doi.org/10.13995/j.cnki.11-1802/ts.025106
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    Zhacai is a popular fermented product made from Brassica vegetable in China. The prokaryotic microbial diversity and physicochemical properties during the fermentation process of Zhacai were investigated using 16S rDNA-based high-throughput sequencing and physicochemical methods. The results showed that the relative abundance of Lactobacillus, which was the dominant genus, increased from 0.92% to 74.77% during fermentation. Leuconostoc could be used as a starter with the relative abundance of 3.61% at the beginning of fermentation, which increased rapidly in the early stage of fermentation and thereafter decreased continuously in the later stage of fermentation. Furthermore, the redundancy analysis showed that the prokaryotic microbial communities were positively correlated with pH value and reducing sugar contents in the early stage of fermentation, and positively correlated with the contents of nitrite, pyruvic acid and succinic acid in the meta stage of fermentation, while they were positively correlated with the levels of total acid and other organic acids (malic acid, tartaric acid, citric acid, oxalic acid, acetic acid, lactic acid) in the later stage of fermentation. The successions of prokaryotic microbial communities and its influencing factors were systematically studied in this work, which could be a theoretical basis for further precise artificial regulation and data reference for screening characteristic strains.
  • WANG Jungang, LI Yuhui, LIU Chengjiang, GUO Anmin, YUE Janping
    Food and Fermentation Industries. 2021, 47(1): 35-42. https://doi.org/10.13995/j.cnki.11-1802/ts.025181
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    The aim of this study was to elucidate the structure of fungi in traditional dried meat of Kazak nationality in Xinjiang,the gene sequences of internal transcribed spacer 1 (ITS1) of fungi in traditional dried meat samples of Xinjiang were analyzed by IlluminaMiSeq high-throughput sequencing technology.The results showed that a total of 1 936 392 gene sequence bands were obtained from 26 samples in four groups,and 371 operational taxonomic unit (OTU) numbers were obtained.The total number of OTU in four groups was 84,and the total number of OTU in 26 samples was only 13.The dominant fungi at genus level were Candida 30.09%,Mrakia 16.26%,Debaryomyces 11.14%,Mycosphaere 6.37%,Penicillium 4.23%,Aspergillus 2.39%,Mrakiella 2.88%,Naganishia 1.71% and Vishniacozyma 1.55%.At the level of phylum classification,the structure of fungal flora in air-dried meat was similar to that of soil,air,and water.At the genus level,Candida,Debaryomyces and Aspergillus had developed into dominant bacteria.These fungi could produce proteases and lipases,which are critical to the quality of meat products during fermentation.The fungal flora in Xinjiang traditional air-dried meat is rich in diversity,and most of them play a positive role in meat fermentation.High-throughput sequencing technology can monitor the fungal diversity in traditional air-dried meat.
  • ZHAO Yifan, CHANG Xiaojiao, DU Wen, SUN Changpo, LIU Hujun
    Food and Fermentation Industries. 2021, 47(1): 43-49. https://doi.org/10.13995/j.cnki.11-1802/ts.024449
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    In order to improve the yield of fumonisin B1 (FB1) carboxylesterase produced by recombinant Pichia pastoris in shaking flask, the fermentation condition and medium were optimized with enzyme activity as index. The fermentation conditions were optimized through single factor experiment. The key components of fermentation medium were screened through Plackett-Burman experiment and then orthogonal experiment was carried out to establish the experimental data samples. Finally, the error Back Propagation (BP) neural network model was established to predict and search for the optimal composition of fermentation medium. After optimization, the optimal fermentation conditions were induction temperature 28 ℃, initial pH 6.0, 1% of methanol addition every 24 h, induction for 96 h. The optimal fermentation medium was peptone 23 g/L, KH2PO4 44 g/L, PTM4 1.5 mL/L, K2SO4 7.15 g/L, MgSO4·7H2O 5.85 g/L, yeast extract 5 g/L. FB1 carboxylesterase activity was over 402 U/mL, 1.19-fold higher than that of before optimization. Fermentation optimization in shaking flask can significantly improve the enzyme production capacity of recombinant strains. These results presented basic data for the optimization of FB1 carboxylesterase in fermenter.
  • LOU Zhihua, LIU Xiang, ZHANG Jingnan
    Food and Fermentation Industries. 2021, 47(1): 50-54. https://doi.org/10.13995/j.cnki.11-1802/ts.025483
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    To investigate the heterologous expression in Bacillus licheniformis and improve the fermentation level of maltotetraose-forming amylase, a xylose-inducible expression system was constructed in B. licheniformis CICIM B1391 to express the maltotetraose-forming amylase from Pseudomonas saccharophila, and a preliminary fermentation optimization was conducted. It was observed that maltotetraose-forming amylase was successfully secreted into the fermentation broth induced by xylose. After optimized induction time, temperature and fermentation time, the maximum enzyme activity reached to (168.2±9.41) U/mL in shake flask fermentation. The optimal fermentation conditions were adding the inducer at 24 h, the induction temperature of 30 ℃, and ending fermentation at the end of stable period.
  • YOU Ling, TAN Yi, LONG Qingyang, CHEN Hong, ZHOU Rongqing, ZHAO Dong
    Food and Fermentation Industries. 2021, 47(1): 55-61. https://doi.org/10.13995/j.cnki.11-1802/ts.024565
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    In order to explore the roles of Geotrichum sp. in the strong-flavor Baijiu fermentation, two strains were isolated from the fermented grains of strong-flavor Baijiu. Their growth and metabolism characteristics were studied. It was found that the similarity of 26S rDNA sequence between the two isolated strains and four strains of Geotrichum was higher than 99%, and the utilization of sugars were also similar to Geotrichum sp., while could not form pseudohyphae. The two strains could tolerate pH 4.0 and 6% vol ethanol, and the optimum growth temperature was 28 ℃ in L-YPD medium. In the fermented grains, the two isolated strains started the slow but constantly fermentation ethanol production was 7.91 and 6.71 mL/100 g, respectively, which was 2.5 and 4.5 times of liquid fermentation, while the total yield of flavor substances was 91 and 340 times of liquid fermentation, respectively. They produced 1.5 and 3.0 g/kg ethyl acetate, 216 and 573 mg/kg isoamyl acetate, 658 and 477 mg/kg β-phenylethanol in grains. The Geotrichum sp. grew well in the fermented grains of strong-flavor Baijiu, with constant fermentation and produced more ethanol than that of commercial yeast, and could improve the flower and fruit aroma of strong-flavor Baijiu, thus showed application prospect of new commercial yeast for Baijiu.
  • TANG Ke, ZHANG Xiaoqian, LI Jiming, JIANG Wenguang, XU Yan
    Food and Fermentation Industries. 2021, 47(1): 62-70. https://doi.org/10.13995/j.cnki.11-1802/ts.024802
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    In order to investigate the evolution of aroma compounds during fermentation, Vidal ice wine fermented by R2 yeast was selected and the volatile compounds during fermentation were determined by headspace solid phase microextraction combined with full two-dimensional gas chromatography mass spectrometry. The results showed that the volatile components of ice wine changed significantly during the fermentation. The total contents of alcohols, aromatics, esters, acids, terpenes, phenols, nitrogen-containing, furans, lactones and aldehydes compounds began to increase gradually, and reached the highest concentration on the 14th day of fermentation, and then decreased and tended to be stable; the total content of ketones decreased with fermentation, and tended to stabilize after 14 days of fermentation; sulfur-containing compounds gradually increased until the 28th day of fermentation, and then decreased. In addition, the partial least squares discrimination analysis results showed that the volatile compounds changed significantly at the early stage of fermentation (0 d, 7 d and 14 d), but the samples were more aggregated in the middle and late stage of fermentation, and the volatile compounds tended to be stable. This study is expected to provide basic data and theoretical basis for further regulation of flavor in ice wine production.
  • HAO Nan, MA Tengzhen, YANG Xueshan, ZHANG Bo, HAN Shunyu
    Food and Fermentation Industries. 2021, 47(1): 71-78. https://doi.org/10.13995/j.cnki.11-1802/ts.023194
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    Pediococcus parvulus C30 from Gansu Hexi Corridor was used as a malolactic fermentation starter for Cabernet Sauvignon wine, and the effect of malolactic fermentation on the wine quality by P. parvulus C30 of different inoculation methods were compared. P. parvulus C30 was inoculated at the early, middle and late period of the fermentation using commercial lactic acid bacteria as a control. The results showed that the shortest duration to complete the malolactic fermentation was 27 days when P. parvulus C30 was inoculated in the late period of the alcoholic fermentation. The contents of polysaccharides, biogenic amine, other substances, reducing sugar and alcohol degree met national standards. The total contents of aroma components in wine samples fermented with P. parvulus C30 were significantly higher compared to commercial strains. In conclusion, P. parvulus C30 can complete malolactic fermentation. It has excellent performance in malolactic fermentation when inoculated in the late period of alcohol fermentation, and it has a positive impact on the quality of Cabernet Sauvignon wine.
  • WANG Jing, LI Min, GAO Pingping, ZHAO Mei, YANG Jie
    Food and Fermentation Industries. 2021, 47(1): 79-87. https://doi.org/10.13995/j.cnki.11-1802/ts.025161
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    To study the application potential of Lachancea thermotolerans and Torulaspora delbrueckii in ice wine fermentation, growth parameters including killer property, growth dynamics, tolerance, and fermentation properties of three L. thermotolerans strains (LT1, LT2, LT3) and three T. delbrueckii strains (TD1, TD2, TD3) collected from different sources were evaluated. The results showed that all the tested L. thermotolerans and T. delbrueckii strains had good adaptability for ice wine fermentation conditions, and strains LT2 and TD2 revealed good tolerance to 400-500 g/L glucose, 15% alcohol content, 400 mg/L SO2 at 13-19℃. In addition, strain TD2 and the three L. thermotolerans strains showed strong killer activity to sensitive Saccharomyces cerevisiae strain 1296. In the ice wine fermentation, LT2 strain had the highest lactic acid production (2.195 g/L). The alcohol content of ice wine fermented by LT2 and TD2 strains can reach 11%-12% vol, and the total contents of volatile esters and fatty acids in the ice wine were significantly reduced than the wine samples fermented by Saccharomyces cerevisiae, but the content of volatile terpenes were significantly increased. Sensory analysis showed that the LT2 strain enhanced the acidity to balance the sweetness, helping to harmonize the taste of ice wine, while the TD2 strain strengthened the flower and fruit aroma of the ice wine. It was suggested that either LT2 or TD2 inoculated fermentation have certain positive effects on the sensory quality of ice wine, both L. thermotolerans and T. delbrueckii have the potential to brew ice wine.
  • WU Yuxia, LIU Yulian, MA Chaoling, MAO Juan, YANG Jiangshan, WANG Yanxiu, CHANG Yongyi
    Food and Fermentation Industries. 2021, 47(1): 88-96. https://doi.org/10.13995/j.cnki.11-1802/ts.024584
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    The quality of grape berries is greatly affected by the ecological environment.Under the delayed cultivation conditions in the alpine desert, the quality of berries is significantly different from that in open cultivation.The aim of this research is to verify and Compare the adaptability and quality performance of eight cultivars of table grape.It could provide a theoretical reference for the variety selection and quality identification of delay-cultivated grape in the alpine desert.Under the delayed cultivation conditions, the eight grape cultivars were preliminarily selected as the research object.The test cultivars were collected from the introduction greenhouses located in Tianzhu county.The experiment determined the berry sensory quality, intrinsic quality, efficacy components, sugar and organic acid composition and content.In order to improve the credibility of the analysis, the method of principal component analysis was adopted.The comprehensive quality of 8 cultivars were ranked according to the comprehensive scores.The results showed that, the fruit mass, vertical and horizontal diameter of ‘Red Globe’ and ‘Zidiqiu’ could maintain the original characteristics of large fruits.The berries of the seed cultivars were larger than the seedless.The fruit shape index of 8 cultivars was greater than 1, which were oval.Except for ‘Ruidu Xiangyu’, the peel color of other cultivars was darker.The darker the peel, the higher the content of anthocyanins, flavonoids and total phenols.In terms of intrinsic quality and active ingredients, ‘Ruby Seedless’, ‘Zitian Seedless’, and ‘Red Globe’ had higher soluble solids content, with an average content of more than 21%.No sucrose was detected in berry of 8 cultivars.The sugar components were mainly fructose and glucose.Glucose content was slightly lower than fructose.The main organic acids were tartaric acid and malic acid, and the content of citric acid and oxalic acid were extremely small.Fruit sweetness value showed that the taste ‘Ruby Seedless’ was the sweetest, followed by ‘Red Globe’, and the worst were ‘Moldova’.Using the method of principal component analysis, the quality indexes were converted into 5 independent principal components, which cumulative contribution rate was 93.150%.‘Ruby Seedless’, ‘Red Globe’ and ‘Muscat Hamburg’ had the best comprehensive quality among the 8 cultivars.
  • SUN Ye, LI Yinghao, WULANDARI, LYU Lishuang, ZHANG Qiuting
    Food and Fermentation Industries. 2021, 47(1): 97-106. https://doi.org/10.13995/j.cnki.11-1802/ts.025020
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    In order to explore the roles of ultrasonic pretreatment in the preparation of zein nanoparticles (ZNP) by anti-solvent method, the formation of composite particles by ZNP and flaxseed gum (FSG), and the impact on the stability of the Pickering emulsion, the intrinsic fluorescence, secondary structure, particle size and potential of ZNP were analyzed.The results show that ultrasonic pretreatment of zein ethanol solution can significantly reduce the average particle size of ZNP and increase the absolute value of ZNP potential.It was also found that the particle size of ZNP further decreased with the increasing ultrasound time at lower ultrasonic power density (230 W/cm2), the absolute value of the potential increased, that is, the stability increased.However, when the ultrasonic power density up to 460 W/cm2, the particle size of ZNP increased slowly, and the absolute value of potential decreased.Then, ZNP pretreated by ultrasound were mixed with FSG by mass ratio of 1∶1, which stated ultrasound treatment make ZNP combine more FSG by surface hydrophilicity analysis.The surface potential of the ZNP was increased, which promoted more FSG to bind to the surface of the ZNP, resisting the aggregation and breakage of droplets in the emulsion.At the same time, through the study of thermal stability, it was confirmed that ultrasonic pretreatment can significantly improve the stability of Pickering emulsion.Therefore, the results of this study can provide a certain basis for the application of ultrasonic pretreatment in the preparation of ZNP and Pickering emulsion, and provide a new green way for the preparation of Pickering particles with higher efficiency and thermal stability.
  • PENG Songlin, ZHANG Yinong, ZHAO Ziyue, LI Yixuan, KANG Mengyao, SHANG Yongbiao
    Food and Fermentation Industries. 2021, 47(1): 107-115. https://doi.org/10.13995/j.cnki.11-1802/ts.024968
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    This paper aims to explore the influence of heterogeneous liquid system conditions on the emulsifying effect and improve the emulsifying effect of oil.Sodium caseinate was used as emulsifier to study the effects of different system conditions (protein dosage, fat-water ratio, pH, NaCl concentration) on the characteristics of soybean oil emulsion.The results showed that in the range of 0.1%-0.5%, the viscosity of emulsion and the adsorption capacity of interfacial proteins were significantly increased with the increase of protein addition.The average particle size of the droplet decreased from 60 μm to 7.28 μm, and the range of particle size distribution became narrower.In the range of 1∶2-2∶1, the viscosity of emulsion increased with the increase of the ratio.When the ratio of lipid water was larger than 1∶1, the viscosity was about 5 times higher than that of other groups, which was found to be related to the compact accumulation of milk droplets through the microscopic structure diagram.Except that the lipid/water ratio of 2∶3 and 1∶1 did not change significantly, the interfacial protein adsorption of emulsion showed an increasing trend.When the fat-water ratio was less than 1∶1, there was little difference in the average particle size and distribution range of the droplets, and then the particle size first decreased and then increased.When the pH of emulsion ranged from 3 to 11, the emulsion droplet aggregation was serious and the average particle size and distribution range were higher than those of other groups.The addition of NaCl can significantly improve the emulsion viscosity and interfacial protein adsorption, 0.6 mol/L NaCl concentration or less, the emulsion particle size is less than the control group, the concentration of 0.12 mol/L reached the minimum value, but is 0.84 mol/L NaCl concentration in larger emulsion droplet diameter emulsion droplet, the average particle size than the control group, acuity 0.36 mol/L NaCl concentration emulsion zeta potential absolute value smaller than in the control group.The appropriate conditions for emulsification are:casein addition amount 0.5%, fat-water ratio 3∶2, pH 7-9, and NaCl concentration≤0.6 mol/L.
  • BI Rixiu, LU Jing, ZHANG Shuwen, PANG Xiaoyang, HU Cheng, YU Jinghua, LYU Jiaping
    Food and Fermentation Industries. 2021, 47(1): 116-124. https://doi.org/10.13995/j.cnki.11-1802/ts.024695
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    The changes of Maillard reaction by-products in different commercial brown yoghurt under different storage conditions were studied. The results indicated that the Maillard reaction mainly occurred in the early stage during storage, and the content of by-products increased with the increase of temperature in the early and advanced stages. However, the rate of Maillard reaction in the advanced and final stages was slow, and it was mainly affected by storage time. With the extension of storage time, the by-products of advanced and final reactions accumulated, but the color change was not significant. Maillard reaction was still in progress during the storage of brown yogurt. The degree of reaction was affected by different storage conditions. There were differences in the content of by-products at different reaction stages in the later storage. It is suggested that the storage temperature of brown yogurt should be strictly controlled at 4 ℃ and consumed within the shelf life. In addition, it is found that the Maillard reaction degrees for different brands of brown yogurt are different, which is related to the process conditions and the content of physicochemical indexes of brown yogurt. Therefore, the Maillard reaction degree should be controlled to reduce the production of potential harmful by-products while optimizing the product process.
  • KONG Qingmin, ZHU Huiyue, TIAN Peijun, ZHAO Jianxin, ZHANG Hao, CHEN Wei, WANG Gang
    Food and Fermentation Industries. 2021, 47(1): 125-131. https://doi.org/10.13995/j.cnki.11-1802/ts.024501
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    To evaluate the effects of Lactobacillus acidophilus La28 on relieving peripheral inflammation and improving liver function in rats induced by valproic acid, Wistar rats were intraperitoneally injected with VPA or normal saline on day 12.5 after pregnancy and the male offspring were randomly grouped as the control group, VPA group, La28 group and risperidone (MED) group (n=5). La28 group was treated with L. acidophilus La28 (109 CFU/mL) by gavage for 28 days. The control, VPA and MED groups were treated with equivalent saline and risperidone by gavage. After the intervention, peripheral inflammation, liver function and gut microbiota were evaluated. Compared with rats in VPA group, L. acidophilus La28 significantly improved peripheral inflammation and liver function in rats. The effects on IL-6, TNF-α, alanine aminotransferase (ALT) andγ-glutamyl transpeptidase (GGT) were more significant. In addition, L. acidophilus La28 might improve the peripheral inflammation and liver function through regulating the relative abundance of Allobaculumhe and Mucispirillum, increasing Firmicutes abundance, and decreasing Bacteroides abundance.
  • DONG Chenyang, ZHANG Hongxing, JIA Yu, XIE Yuanhong, LIU Hui, JIN Junhua
    Food and Fermentation Industries. 2021, 47(1): 132-137. https://doi.org/10.13995/j.cnki.11-1802/ts.023705
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    This study investigated the antioxidant function of Lactobacillus salivarius M18-6 bacterial suspension, cell-free supernatant and cell contents in vitro, respectively, by testing 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) clearance rate, inhibition of linoleic acid peroxidation rate, hydroxyl radical (·OH) clearance rate, antioxidant related enzyme activity, etc. Furthermore, combined with strain genome information, this study detected the transcription level of 11 genes involved in antioxidant function using real-time fluorescence quantitative PCR. The results showed that activity of scavenging DPPH free radicals and ·OH, and inhibiting linoleic acid peroxidation rate were significantly higher than that of L.rhamnosus GG (P<0.05). Meanwhile, the glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activities were also high in the cell-free supernatant of the strain. The results indicated that the cell-free supernatant of L. salivarius M18-6 had strong antioxidant capacity. Under 2.5 mmol/L H2O2 stress, the transcription level of the genes encoding NADH oxidase and superoxide dismutase of the L. salivarius M18-6 were up-regulated by 6.2 and 4.5 times, respectively, and the transcription level of thioredoxin series genes (trxA1, trxA2, trxA3, trx) and catalase(cat) were also up-regulated by more than 2 times. It is speculated that L. salivarius M18-6 might exhibit antioxidant effects by increasing the expression of NADH oxidase and thioredoxin to activate the thioredoxin system, and by up-regulating the expression levels of sod and cat genes to increase the activity of SOD and catalase.
  • GAO Xuejing, LUO Siyi, TANG Kaijie, LUO Qiushui
    Food and Fermentation Industries. 2021, 47(1): 138-142. https://doi.org/10.13995/j.cnki.11-1802/ts.024873
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    The hypolipidemic and antioxidant activity of crude glycoprotein from hawthorn in high-fat mice were investigated in the paper.The active ingredients were extracted by water extraction and alcohol precipitation, the free protein was removed by Sevage method, DEAE-52 cellulose chromatography and SephadexG-75 purification were used to prepare crude hawthorn glycoprotein.KM mice were selected as the research objects, and randomly divided into blank control group, high-fat model group, hawthorn glycoprotein low, medium and high dose groups.After 8 weeks of gavage, the content of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) in serum were measured, the activities of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and the content of malonaldehyde (MDA) in liver were measured.Results showed that compared with the blank control group, the levels of TC, TG and MDA in the high-fat model group increased, while HDL-C, CAT, T-SOD and T-AOC decreased, all the differences were significant (P<0.01).Compared with the high-fat model group, the content of TC, TG and MDA in the hawthorn glycoprotein group decreased (P<0.05), and the content of HDL-C and the activities of CAT, T-SOD and T-AOC increased (P<0.05).The dose-effect relationship was significant, from this we can find that the crude glycoprotein from hawthorn had a better effect on hypolipidemic and antioxidant activity in high-fat mice.
  • GONG Chunyu, LIU Yuting, SHAN Jiaming, XU Shuo, XING Yue
    Food and Fermentation Industries. 2021, 47(1): 143-147. https://doi.org/10.13995/j.cnki.11-1802/ts.024495
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    By ethanol precipitation, the polysaccharide (CSP) extracted by ultrafiltration from Stigma maydis was isolated to three fractions, CSP-1, CSP-2 and CSP-3.The effect of this four kinds of polysaccharides on humoral and cellular immune functions and mainly organs in mice were studied.The results showed that CSP mainly contained CSP-2 which was precipitated by 80% ethanol.CSP, CSP-1 and CSP-2 could effectively improve antibody production and humoral immunity, activate macrophage,improve macrophage phagocytosis, but CSP-3 only could improve humoral immunity.CSP, CSP-1 and CSP-3 could increase immune organs index in mice.But all kinds of polysaccharides could not increase the heart, kidney, lung, liver and brain growing.Compared with each other, CSP-1 has a better activating effect on humoral immunity function than CSP-2 and CSP-3.CSP-2 has a better activating effect on cellular immune function than CSP and the positive control.
  • GAO Jian, LIN Wei, LIU Xiaolan, LIU Xiang, ZHENG Xiqun, LI Guanlong
    Food and Fermentation Industries. 2021, 47(1): 148-154. https://doi.org/10.13995/j.cnki.11-1802/ts.024921
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    In order to expand the application of corn gluten meal(CGM) in the field of functional food, the effects of corn gluten hydrolysate (CGH) on spleen lymphocyte proliferation in vitro, immune regulation and antioxidant activities in immunosuppressed mice were studied.CGH was obtained from CGM by synergistic hydrolysis catalyzed by protamex and alcalase.The immunoactivity of CGH was determined by the proliferation of spleen lymphocytes in vitro, then, an immunosuppressive mouse model was constructed.Mice were given CGH by gavage and obtained with cyclophosphamide at twenty-first day.Cytokines (TNF-α, IFN-γ, IL-2 and IL-6), the antioxidant enzymes activities (SOD, GSH-Px, CAT) and malondialdehyde (MDA) content were measured.The results showed that CGH at concentration between 80 μg/mL and 320 μg/mL could promote the proliferation of spleen cells cultured in vitro.Compared to the model group, the high, medium and low doses [200, 100, 50 mg/(kg·d)] could significantly increase the content of TNF-α and IFN-γ;the high and medium doses could significantly increase the content of IL-2;the high dose could significantly increase the content of IL-6 (P<0.05).The high and medium doses [200, 100 mg/(kg·d)] could significantly increase the contents of SOD, GSH-Px, CAT and could significantly decrease the content of MDA (P<0.05).Therefore, CGH has a good immunomodulatory effect in vitro and in vivo, and is a potential immunomodulator, which lays a foundation for the application of immunomodulatory peptides from corn gluten meal in functional foods.
  • CHEN Qiong, CHEN Peng, LI Junying, XU Minjun
    Food and Fermentation Industries. 2021, 47(1): 155-159. https://doi.org/10.13995/j.cnki.11-1802/ts.025185
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    To investigate the effect of the compound formula of Semen Ziziphi Spinosae extract and theanine on mice’s sleeping quality and its mechanism. Male Balb-c mice were randomly divided into 3 groups, low-dose group (Semen Ziziphi Spinosae extract: 105 mg/(kg·d), theanine: 58 mg/(kg·d), high-dose group (Semen Ziziphi Spinosae extract: 158 mg/(kg·d), theanine: 87 mg/(kg·d), and control group (distilled water) for 30 d continuous intragastric administration. The sleeping indicators and some indexes in blood and brain tissue were measured. The results showed that, compared with the control group, the hypnotic test sleep rate and the brain 5-hydroxytryptamine concentration were significantly increased, while the brain β-endorphin concentration was significantly decreased both in low-dose group and high-dose group. Besides, mice in the high-dose group had prolonged sleeping time (P<0.01), shortened sleeping latency (P<0.01), and higher blood tryptophan concentrations (P<0.05) compared to the control group. The results suggested that Semen Ziziphi Spinosae extract and theanine compound formula improved the sleeping quality with a dose of 158 mg/(kg·d) Semen Ziziphi Spinosae extract and 87 mg/(kg·d) theanine. It may be achieved by affecting the tryptophan concentration in mouse blood, the 5-hydroxytryptamine concentration and β-endorphin concentration in mouse brain.
  • GE Xiangyang, HE Hongkui, LIU Guoying, MA Jintong, SONG Shuyu, GUO Kaikai, XIONG Weinan, LI Anjun, ZHOU Qingwu
    Food and Fermentation Industries. 2021, 47(1): 160-164. https://doi.org/10.13995/j.cnki.11-1802/ts.024488
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    In order to determine metabolism rate of ethanol in human body, breathalyzer was used to track and detect volunteers after drinking beer, wine, and Baijiu(Chinese liquor). The results showed that the average metabolic rate of all volunteers was Baijiu>red wine>beer, and the metabolism rate in the first 2 h was highly positive correlated with that of the following 2 h. Through the analysis of the skeleton flavor substances of typical beer, wine and Baijiu, it was found that alcohols, such as N-propanol, furfuryl alcohol, propylene glycol, N-pentanol, β-phenylethanol were the key flavor substances that affected the early metabolism rate, and N-propanol was the most significantly flavor substance that decreased the metabolism rate.
  • WAN Yin, YU Xinjin, FENG Silin, WANG Jiahuai, XIAO Hui, LEI Yu, PI Xiaowen, LI Wenhui, FU Guiming
    Food and Fermentation Industries. 2021, 47(1): 165-171. https://doi.org/10.13995/j.cnki.11-1802/ts.024554
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    In order to develop dried bean curd sticks with low purine, resin adsorption combined with nuclease enzymatic hydrolysis of nucleic acid were used to remove purine substances in soybean milk. The optimal conditions for the adsorption of resin combined with nuclease hydrolysis were determined by orthogonal experiment. The results showed that NUF20 resin was the best adsorption resin with the adsorption rate of 98.40% on purine and nucleotide. The optimum adsorption condition was obtained when soybean milk was stirred at 70 ℃ for 1 h after mixed with 0.2 g/mL resin and 26.67 U/mL nuclease. After treatment, the purine content of the dried bean curd sticks in the 1st to 5th sheets decreased by 89.70%, 88.28%, 76.69%, 72.03% and 65.62%, respectively. The research combined resin adsorption and nuclease digestion under optimal technical conditions to effectively reduced the purine content of dried bean curd sticks. It provides a technical basis for the development of dried bean curd sticks with low purine content for the special population such as hyperuricemia persons, also it provides a theoretical reference for the research and processing of other low purine bean products.
  • DAI Wenting, WANG Yuan, XING Lijie, WU Hong, KANG Xiaoning, WU Hongbin, WANG Shiping, DAI Jiahui
    Food and Fermentation Industries. 2021, 47(1): 172-179. https://doi.org/10.13995/j.cnki.11-1802/ts.024714
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    In this study, black wolfberry, flat peach and grape were used as the raw materials to optimize the flat peach grape black wolfberry compound beverage recipe, which was aimed to make the sensory evaluation results more scientific and objective and to obtain an optimum formula as well.The single factor tests and the response surface method in combination with fuzzy mathematics comprehensive evaluation method were applied to optimize the compound beverage recipe.The results showed that, when 1% xylitol, 0.001 4% sucralose, 0.001 4% mogroside, and 0.1% citric acid were added to the compound beverage, and the sensory evaluation score was as high as 8.38±0.03, which was close to the theoretical value of the model.The compound beverage displayed a bright color, pleasant aroma, sweet and sour flavor.Meanwhile the acid-sugar ratio was 4.2∶1, and the contents of anthocyanin, total sugar, total acid were 144 μg/mL, 11.2 g /100 g, 3.4 g/100 g, respectively, and it was rich in 13 kinds of amino acids.The experimental results can provide a theoretical basis for the industrial production of flat peach,grape and black wolfberry compound beverage.
  • SUN Liting, LIU Lizeng, LIU Aiguo, WANG Weijia, MA Jiang, YANG Yi
    Food and Fermentation Industries. 2021, 47(1): 180-185. https://doi.org/10.13995/j.cnki.11-1802/ts.024830
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    With ruby plum as experimental raw materials, the preserved fruits were processed by various sugar infiltration technologies such as atmospheric pressure, vacuum, ultrasonic wave and microwave, in order to study how different sugar infiltration methods affect the color, texture characteristics, total sugar content, rehydration, water composition, taste and sensory evaluation of preserved ruby plum.The results demonstrated that vacuum sugar infiltration technology could improve the color brightness of the product significantly, while the color obtained by microwave sugar infiltration was poor.Preserved fruits which applied ultrasonic wave sugar technology had observable higher rehydration rate, hardness and toughness (P<0.05), and the gross sugar content was the highest.The relative content of bound water in the preserved fruit prepared by microwave and ultrasonic wave sugar infiltration technologies was remarkably higher (P<0.05).Moreover, the results of electronic tongue measurement and sensory evaluation showed that the preserved fruit processed by ultrasonic wave sugar infiltration has the best taste.
  • ZHOU Kui, HONG Yan, LIANG Shangyun, ZHANG Yayuan, YOU Xiangrong, LI Mingjuan, WEI Ping, WANG Ying
    Food and Fermentation Industries. 2021, 47(1): 186-192. https://doi.org/10.13995/j.cnki.11-1802/ts.025149
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    The selenium retention and quality of rice flour of spray-drying, vacuum freezing drying and drum drying were studied.It was to develop the Se-rich rice meal replacement powder.The order of the three methods of selenium retention was that spray-drying<vacuum freezing drying<drum drying.The order of the three methods of degree of gelatinization was that raw material<drum drying<vacuum freezing drying<spray-drying.The Se-rich rice flour (selenium content 192.08 μg/kg) prepared by drum drying has lower degree of gelatinization, the highest selenium retention rate (48.82%).The sheet structure showed good solubility.Considering the selenium content, nutritional quality and dissolution stability, results showed that the better formula of meal replacement powder was:Se-rich rice flour 50 g/100 g, jujube powder 5 g/100 g, black sesame powder 0.5 g/100 g, kudzu powder 5 g/100 g, yam powder 24.5 g/100 g, red bean powder 5 g/100 g, coix seed powder 5 g/100 g, brown rice powder 5 g/100 g.
  • DAI Yafeng, WANG Donghui, QING Pengcheng, WU Hao, ZONG Ruiye, MA Shaoshuai, GUO Xiaohui, WANG Zengli
    Food and Fermentation Industries. 2021, 47(1): 193-197. https://doi.org/10.13995/j.cnki.11-1802/ts.024544
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    The aim of the current study was to compare the effects of hot-air drying with different temperatures and the application of color protection on the quality of the Dendrobium huoshanense flower.The best hot-air drying condition was confirmed by comparing moisture ratio, drying rate and main biologically active components (polysaccharides, total phenol, flavonoids) of the Dendrobium huoshanense at different temperatures(50, 60, 70, 80 ℃)and further explore the effects of different color protection.The hot-air drying with 50 ℃ had the longest drying time while drying at 70 ℃ showed the lowest total phenol contents and drying at 80 ℃ showed the lowest contents of flavonoids.After fully consideration both of the drying efficiency and contents of bioactive ingredients, drying with 60 ℃ was selected as the best hot-air drying condition.Specifically, the drying time was 4.5 h drying at 60 ℃ and the contents of flavonoids, polysaccharides and total phenol of dried products were (10.94±0.10), (16.80±2.69) and (28.06±0.14) mg/g, respectively.Furthermore, after comparing the color protection effects from various color protection agents, we found the use of 0.5 g/L sodium erythorbate significantly improved the color quality of the Dendrobium huoshanense flower under drying at 60 ℃.The Dendrobium huoshanense flower was pretreated with 0.5 g/L sodium erythorbate and hot-air drying at 60 ℃ for 4.5 h had the best quality in color and bioactive ingredients.
  • ZHANG Yingmin, REN Guangyue, DUAN Xu, LIU Panpan
    Food and Fermentation Industries. 2021, 47(1): 198-205. https://doi.org/10.13995/j.cnki.11-1802/ts.024666
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    To determine ensure the quality of sweet potato leaf powder and reduce processing energy consumption, this study used heat pump-hot air combined drying technology to treat sweet potato leaves, based on a single factor test, the Box-Behnken Design (BBD) method was used to design and optimize the test to study the heat pump drying temperature, The influence of hot air drying temperature and moisture content of conversion point on unit energy consumption, chlorophyll content, color L* value and hygroscopicity, a polynomial regression model is derived by weighted comprehensive scoring method, and the parameters of combined drying process are optimized.The optimized parameters of the response surface are:heat pump drying temperature 52 ℃, hot air drying temperature 73 ℃, conversion point moisture content 58%, unit energy consumption under this process is 3 621.36 kJ/g, chlorophyll content 6.42 mg/g, color L value 46.21, moisture absorption 7.19%, and the comprehensive score value and the predicted value are as high as 99.63%, which lays a theoretical foundation for the comprehensive utilization of sweet potato leaves.
  • DUAN Zhirong, JIN Miaomiao, WU Shanhong, DAI Hui, ZHANG Min
    Food and Fermentation Industries. 2021, 47(1): 206-213. https://doi.org/10.13995/j.cnki.11-1802/ts.024480
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    The germination time of sweet potato which not treated with exogenous ethylene was taken as the node.The concentration of 1 g/L exogenous ethylene was treated before the node, after the node and during storage and transportation, respectively.To study the effects of exogenous ethylene treatment timing on the regulation of germination and carbohydrate metabolism of sweet potato during ambient temperature storage and transportation.The results revealed that ethylene treatment could promote the respiration of sweet potato, decrease the content of tuberous starch and sugar, and increase the activation of α-amylase and β-amylase.Ethylene treatment with the dose of 1 g/L of whole process or post the node can better inhibit sweet potato sprout, and can significantly enhance the sucrose phosphate synthase (SPS) and the neutral invertase(NI) of sweet potato, reduce the acid invertase (AI), maintain higher soluble total sugar and sucrose content.Thus, sweetness of sweet potato would be higher.
  • CHEN Cong, HU Changli, XIE Jing
    Food and Fermentation Industries. 2021, 47(1): 214-221. https://doi.org/10.13995/j.cnki.11-1802/ts.024922
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    In order to investigate the effects of concentrating and freezing methods on milk quality, the milk was concentrated into samples of different total solids content (11.83%, 14.94%, 17.61%, 23.01%) by membrane separation.Milk samples were frozen in four ways (general refrigerator, cold storage, spiral freezer, and plate freezer).The indices were determined by color, pH, titratable acidity, particle size, ethanol stability and heat stability, and the combination of confocal microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were also measured.The results showed that with the increase of total solids content, the pH value, titratable acidity, ethanol stability and heat stability of milk decreased, and the total color difference value and mean particle diameter increased.β-lactoglobulin content in milk samples decreased after freezing treatment.Among the four freezing methods, the plate freezing rate was the fastest, and ethanol stability and thermal stability were the best.The mean particle size of general refrigerator frozen milk and the size of milk fat globules were the largest.In conclusion, milk samples concentrated to a total solids content of 23.01% and frozen with a plate freezer were recommended.
  • MA Yifei, LIU Huan, SHAN Qianyi, REN Shan, BAO Jianqiang
    Food and Fermentation Industries. 2021, 47(1): 222-228. https://doi.org/10.13995/j.cnki.11-1802/ts.025025
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    In this study, five different thawing methods, including low-temperature air thawing, water soaking thawing, flowing water thawing, microwave thawing and ultrasonic thawing, were individually used to thaw frozen fish.The fish was treated with two different freezing methods, ordinary freezing and liquid immersion rapid freezing.The thawing time, color, pH, texture, malondialdehyde (MDA) content, sufhydryl content and other indexes of thawed fish were measured and compared.And the texture indexes such as the hardness and chewiness of fish meat after thawing were measured.The results showed that the microwave thawing rate was the fastest among the five thawing methods, and the low-temperature air thawing rate was the slowest.For little yellow croaker treated with quick freezing and ordinary freezing, ultrasonic thawing could effectively maintain the quality of the fish after thawing.
  • WANG Fang, WANG Hongbo, XI Bin, YANG Xiaoling, LI Weihong, GAO Yaqing
    Food and Fermentation Industries. 2021, 47(1): 229-235. https://doi.org/10.13995/j.cnki.11-1802/ts.024899
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    The study was conducted to evaluate the effect of different sheep (Tibetan sheep, Ujumqin sheep and Hu sheep) on physical indexes, nutrition ingredients, amino acids and fatty acids according to the national standard.The results showed that the cooking hooding percentage of Tibetan mutton was 64.74%, which was significantly higher than that of the other two types of mutton (P<0.05) and the protein content was the highest (19.45%).The shear force of Ujumqin mutton was 61.03 N, which was significantly higher than that of the other two types of mutton (P<0.01) and the water content was the highest (76.42%).The ash content and fat content of Hu mutton were significantly higher than those of Tibetan mutton and Ujumqin mutton (P<0.01).The proportion of essential amino acids in total amino acids in Tibetan mutton, Ujumqin mutton and Hu mutton were 41.41%, 39.90% and 40.59%, respectively.The SFA/UFA ratios were 0.85, 1.07 and 0.88 respectively, which were close to the 1∶1 fatty acid composition ratio recommended by the Nutrition Association.In conclusion, Tibetan mutton showed good meat quality in terms of eating quality, nutritional quality and the composition ratio of amino acids and fatty acids.
  • HAN Fang, LI Jingling, YUAN Jing, YU Jie
    Food and Fermentation Industries. 2021, 47(1): 236-242. https://doi.org/10.13995/j.cnki.11-1802/ts.024509
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    In order to study the composition of water shield flavonoids and their antioxidant and antibacterial activities in vitro, in this study, the composition and content of flavonoid in water shield from four major producing areas in China were determined by ultraviolet-visible spectrophotometry (UV-VIS) and high performance liquid chromatography (HPLC).Three antioxidant methods, DPPH, ABTS and FRAP were used to evaluate the antioxidant capacity of flavonoids in water shield from various regions.The filter paper diffusion method was used to determine the antibacterial effects of flavonoids from water shield on Proteus vulgaris, Escherichia coli and Pseudomonas aeruginosa.The results showed that the content of total flavonoids in Shizhu water shield was the highest (42.505±0.458) mg/g, and West Lake was the lowest (15.845±0.119) mg/g.A total of 7 flavonoids were detected in the test materials, mainly isorhamnetin, quercetin and kaempferol.Water shield in each production area has good antioxidant activity and different degrees of antibacterial activity, and the order of their size changes is the same:all are:Shizhu﹥Lichuan﹥Leibo﹥West Lake.This will provide reference data for further research on water shield flavonoids.
  • FAN Shanshan, GUAN Guikun, SU Yazhi, WAN Ziran, LIU Mingkun, WANG Tao, GUO Yimin, XU Yan, FAN Wenlai, CHEN Shuang
    Food and Fermentation Industries. 2021, 47(1): 243-249. https://doi.org/10.13995/j.cnki.11-1802/ts.025000
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    The sensory-directed analysis method was used to analyse the aroma-active components of Lanling Meijiu, a northern style Chinese rice wine. Firstly, the aroma profile was constructed by quantitative descriptive analysis. The results showed that Lanling Meijiu had strong soy aroma, floral, alcoholic and caramel notes. A total of 50 aroma compounds were identified by headspace solid phase microextraction and liquid-liquid extraction combined with gas chromatography-olfactometry-mass spectrometry (GC-O-MS), including esters, alcohols, acids, phenols, aldehydes and ketones. Based on the precise quantification and odor activity value (OAV) calculation, ethyl acetate, ethyl butanoate, ethyl valerate, ethyl hexanoate, ethyl octoate, isoamyl alcohol, phenethyl alcohol, benzaldehyde, phenylacetaldehyde, vanillin, γ-nonalactone and 2-methoxy-4-vinylphenol could be potentially important to the overall aroma profile of Lanling Meijiu.
  • ZHOU Qi, YI Xin, OUYANG Zhu, LI Guijie, FENG Yu, LI Guijie, TAN Anqun, HUANG Linhua, WANG Hua
    Food and Fermentation Industries. 2021, 47(1): 250-258. https://doi.org/10.13995/j.cnki.11-1802/ts.025216
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    The specific differences of aroma components in sweet orange juice and mandarin juice were compared in order to provide further data support for the juicing of mandarin. Juices were extracted from 8 varieties of common sweet orange and mandarin citrus. The aroma components of juice were qualitatively and quantitatively analyzed by using headspace-solid-phase-microextraction-gas-chromatography-mass-spectrometry (HS-SPME-GC-MS) and principal component analysis (PCA) was used to compare and distinguish. A total of 77 aroma components were detected in 16 kinds of citrus juice, including 14 kinds of unique aroma components in sweet orange juice and 7 kinds of unique aroma components in mandarin juice. By calculating OAV, 47 characteristic aromas were obtained, of which linalool, hexanal and nonanal contributed greatly to the main aromas of the two types of citrus juice. Taking the citrus juice sample as the object, 31 aroma substances were selected for principal component analysis, and the two types of citrus juice can be clearly distinguished. The types and contents of terpenoid compounds in the two varieties of citrus juice are the most, and the types and contents of ester and alcohol compounds in sweet orange juice are much higher than those in mandarin juice.
  • WANG Bo, YANG Hongyao, LU Fenggui, CHEN Zidong, CAO Zhenxia, LIU Dengyong
    Food and Fermentation Industries. 2021, 47(1): 259-265. https://doi.org/10.13995/j.cnki.11-1802/ts.024419
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    To explore the machine vision model that can quickly and accurately identify all the colors of smoked chicken legs during the smoking process, this article builds an Xception-CNN model for the recognition of smoked chicken leg colors based on machine vision technology, while compares and analyzes the Xception-CNN model’s recognition effect on smoked chicken leg color by applying ResNet-50, Inception and traditional CNN and other three models.A total of 4 352 smoked chicken leg images of different colors were collected and image pre-processed as experimental samples of four models, of which 3 482 were randomly selected as the training group, and the remaining 870 as the test group.The average recognition accuracy of the four models is Xception-CNN-92%, ResNet-50-91%, Inception-89%, traditional CNN-87%, and test time is Xception-CNN-1.36 s, ResNet-50-0.81 s, Inception-0.98 s, traditional CNN-2.48 s.Therefore, the Xception-CNN model has the highest color recognition accuracy for sugar-smoked chicken leg images, reaching 92%.The test time is slightly higher than the ResNet-50 model and the Inception model, but lower than the traditional CNN model.It only takes 1.36 s to complete the recognition.The fast and accurate identification of the color of smoked chicken legs can provide a reliable basis for the precise control of the parameters of the sugar smoked process and the standardization of product colors.
  • DU Rubing, REN Cong, WU Qun, XU Yan
    Food and Fermentation Industries. 2021, 47(1): 266-275. https://doi.org/10.13995/j.cnki.11-1802/ts.025292
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    Traditional fermented food is increasingly favored by consumers because of its unique flavor and high nutrition.However, the traditional fermented food is usually produced by manual way with natural inoculation resulted in poor stability, low production efficiency and uncontrollable food safety.Therefore, we put forward a novel fermented technology -“Ecological fermentation technology”.Ecological fermentation technology is composed of three major technologies:microbiota deconstruction technology (upstream), core function microbiota recombination technology (middle) and fermentation process control technology (downstream).Ecological fermentation technology is expected to realize the high quality, high efficiency and high safety production of traditional fermented food.This review mainly expounds the necessity, content and future development of establishing ecological fermentation technology.
  • QU Lisha , YU Wenwen, LYU Xueqin, LI Jianghua, DU Guocheng, LIU Long
    Food and Fermentation Industries. 2021, 47(1): 276-284. https://doi.org/10.13995/j.cnki.11-1802/ts.025246
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    Vitamin D is a fat-soluble steroid hormone precursor, which plays an important role in balancing metabolism and maintaining body health. At present, the yield of traditional industrial production methods cannot satisfy the market demand. While, applying synthetic biology to synthesize sterol and using semi-chemical method to synthesize vitamin D is one of the effective ways to solve the contradiction. The research progress of the synthesis of vitamin D by bio-chemical method were reviewed, and dynamic regulation, metabolic compartmentalization and subcellular engineering were especially focused on. Besides, this review puts forward the possible challenges and solutions in the future production.
  • LU Junmeng, ZHAO Xue, XU Xinglian
    Food and Fermentation Industries. 2021, 47(1): 285-292. https://doi.org/10.13995/j.cnki.11-1802/ts.024973
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    The stability of emulsified food products determines its value and customer satisfaction, and emulsifying properties of natural proteins have attracted wide attention in food colloid and interface science for many years.Protein molecules contain both polar and nonpolar groups.The characteristic of amphipathic enables proteins to dispersed in both oil and water phases.Under favorable conditions, proteins in water-oil emulsion can absorb the two-phase interface and organize to form viscoelastic film, which can reduce interfacial tension, resist mechanical force and maintain emulsion stability.Studying the physicochemical properties of the protein interfacial film is helpful to understand the adsorption law of protein macromolecules at the water-oil interface, which can be used to evaluate, characterize and predict the stability of protein emulsion, and provide theoretical support for optimizing the stability of protein emulsion.For these purposes, the structure and influencing factors of protein interfacial film in the oil in water (O/W) emulsion are discussed.The current methods used to study the interfacial properties of proteins were systematically reviewed, including microscopic imaging techniques, thermodynamic methods, spectral techniques and interfacial rheology, in order to understand the relationship between protein-stabilized emulsions properties at a macroscopic and microscopic scale and provide an insight into the application of protein interfacial film in emulsified food.
  • QI Libo, WU Chao, Zhong Limin, SHANG Shan, DONG Xiuping, LIN Songyi
    Food and Fermentation Industries. 2021, 47(1): 293-302. https://doi.org/10.13995/j.cnki.11-1802/ts.026452
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    The shortage of micronutrients is a global problem.In order to improve the nutritional status of poor children,Chinese government have developed an effective nutritional supplement and put forward the concept of “nutrition package”.Infant nutrition packs can be functional and specialized by different ratios of base materials,minerals,vitamins and other components.With the rapid development of nutrition package,the composition of its base material has not been limited to soybean powder,milk powder,etc.,and the use of cereal powder as the base material of nutrition package has emerged.At the same time,functional components such as DHA or prebiotics can be added to the nutrition package for infants and young children.However,during storage and sale,nutrient attenuation and oxidation have been noticed in the nutrition package.Therefore,the microencapsulation method has been applied to improve the stability of attenuated nutrients and fats in nutrient packs.A large number of statistical experiments show that adding nutrition package has a good effect on improving the growth and development of infants,among which the most significant one is improving the anemia status of infants.A low rate of adverse reactions was also found after taking the nutritional package.By reviewing the components,quality controlling,and the functional effects of the nutrition package,this study may provide useful information for the development of the nutrition package.
  • YANG Lianzhan, LI Yan, QIAN Haifeng, ZHANG Hui, QI Xiguang, WANG Li
    Food and Fermentation Industries. 2021, 47(1): 303-308. https://doi.org/10.13995/j.cnki.11-1802/ts.024857
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    Natural plant preservatives are antibacterial substances extracted from different kinds of plants and used as ideal antibacterial and antiseptic resources. As the nutritional and healthy trend of food industry and healthy consideration by consumers, the demand for natural plant preservatives increase rapidly. In this review, the application of natural plant preservatives in animal products, aquatic products, vegetable produces, and wheat-based products are reviewed. Meanwhile, the potential antibacterial mechanisms are discussed, which is helpful to broaden the application of natural plant preservatives in food industry.
  • WANG Shengnan, WANG Xiaoyu, ZHAO Pengtao, LEI Xiaoqing, LIU Pei, ZHANG Qianting, GUO Yurong
    Food and Fermentation Industries. 2021, 47(1): 309-316. https://doi.org/10.13995/j.cnki.11-1802/ts.024557
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    Polysaccharides are one of the important macromolecular substances which can interact with other compounds in wine to improve its stability and sensory quality. The content of various polysaccharides in wine depends on the grape variety, berry ripeness, the wine-making technology, and the wine aging process. The evolution of polysaccharides in wine is unclear because of their complex structure. Moreover, as one of the main methods to improve the quality of wine, the new technology of wine-making has a great impact on the structure and content of polysaccharides. This paper reviewed the changes of polysaccharides during wine-making processes, as well as the effects of grape variety, berry ripeness, origin, vintage, and different wine-making processes on polysaccharides in wine. Furthermore, this study pointed out wine polysaccharides research emphasis in the future, aiming to optimize the wine-making technology and provide a reference to improve the quality of the wine.
  • XIE Jiayu, ZHANG Wen, OU Jie
    Food and Fermentation Industries. 2021, 47(1): 317-326. https://doi.org/10.13995/j.cnki.11-1802/ts.024563
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    Aflatoxin B1 (AFB1) is one of the most carcinogenic chemicals, which has a great threat to human and animal health, especially to liver damage.AFB1 is the most common mycotoxin in cereals and oils, and it is also the most harmful one.And the long-term and low-dose intake of AFB1 will not only cause liver damage and induce liver cancer, but also damage other organs of the human body.Therefore, functional foods and new health medicine that can effectively protect human body from AFB1 damage have become a research focus.In recent years, natural products have attracted much attention due to their advantages of significant biological activities, low side effects and multi-target effects.A variety of natural products of plant origin have been reported to be able to inhibit the growth and toxin production of Aspergillus flavus and the toxic effects of AFB1.In this article, we reviewed the inhibition of plant-derived natural products on the growth and toxicity of Aspergillus flavus and the toxicity of AFB1, as well as the related inhibition mechanism, which will hopefully provide a reference for further research on the protection of AFB1 toxic injury and the development of new health care drugs and functional food that can reduce the toxic injury of AFB1 to human and animals.
  • JI Xianqiu, LUO Xin, ZHU Lixian, LIANG Rongrong, CHEN Xue, HAN Mingshan, ZHANG Wenhua, ZHANG Yimin
    Food and Fermentation Industries. 2021, 47(1): 327-333. https://doi.org/10.13995/j.cnki.11-1802/ts.024809
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    Tenderness is a key indicatorfor evaluating beef quality, which directly affects purchasing decision of consumers.Hence, how to improve beef tenderness has always been the focus of meat research.With the development of science and technology, new technologies of meat tenderization continue to emerge.At present, the newly introduced technologies, such as smartstretch, ultrasound, pulsed electric field, have received extensively attention in the field of beef production.Therefore, based on the recent research results, this paper summarized the tenderization principle, tenderization effectiveness and application prospects of the above three technologies, and found that high-intensity (10-1 000 W/cm2) and low-frequency (20-100 kHz) ultrasound treatment is effective in improving beef tenderness and is an effective beef tenderization technology.The composite application of multiple meat tenderization technologies is also the future development trend of meat industry.
  • GUO Jing, CHEN Yulong, WANG Ping, WANG Ling, CHEN Feiping, LUO Zheng, YU Xin
    Food and Fermentation Industries. 2021, 47(1): 334-340. https://doi.org/10.13995/j.cnki.11-1802/ts.024902
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    Passion fruit is easy to lose water, shrink, rot and deteriorate after harvest because it ripens at high temperature. So it is difficult to store and transport. Therefore, it is of great economic significance to study the postharvest preservation technology and extend the shelf life of passion fruit. In this paper, the postharvest physiological changes and diseases of passion fruit were described, and the research progress of postharvest preservation technology of passion fruit was summarized from the aspects of physics, chemistry, biology and compound preservation. The future development trend of passion fruit preservation technology was prospected, which will provide reference for the in-depth study of postharvest preservation technology of passion fruit.
  • CHEN Yefu
    Food and Fermentation Industries. 2021, 47(1): 341-344. https://doi.org/10.13995/j.cnki.11-1802/ts.025365
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    Chinese liquor culture has a long history with substantial content. Chinese liquor making techniques have great impact on the development of world brewing technology and industrial biotechnology. The course of “Liquor and Culture” is helpful to improve students’ humanistic spirit and scientific literacy, better adapt to social life, and enhance students’ cultural confidence and patriotic feelings. For the purpose of teaching improvement, the connotation of liquor culture, liquor tasting, healthy drinking, ideological and political education, and the origin of alcoholic drink, were discussed, based on the author’s teaching experience of this course.