25 July 2023, Volume 49 Issue 14
    

  • Select all
    |
  • SU Yingying, LIU Song
    Food and Fermentation Industries. 2023, 49(14): 1-8. https://doi.org/10.13995/j.cnki.11-1802/ts.035479
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Gas vesicles, which are composed of gas vesicle protein (Gvp), exist in Cyanobacteria, halophilic Archaea, and other microorganisms, providing buoyancy or stress resistance for cells. Although gvp gene cluster widely exists in Streptomyces strains, its function is still unclear. In this study, the endogenous gvpA, gvpO, and complete gene cluster gvpOAFGJLSK (gvp40587) were expressed in a transglutaminase gene deleted strain (smY2019Δtg) respectively to investigate their effects on the trypsin synthesis and the physiological status of the host. As indicated by enzyme activity analysis, overexpressing gvp40587 and gvpA increased the highest extracellular trypsin activity by 64.3% and 17.0%, respectively; the gvpO overexpression had little effect on trypsin activity. SDS-PAGE analysis showed that the trypsin bands (26 kDa) in the extracellular supernatant of gvp40587 and gvpA overexpression strains were significantly thicker than that of the control strain. In the strain over-expressing the complete gvp gene cluster, the transcriptional levels of the eight gvp genes were increased to different degrees. In addition, the intracellular ATP content and NADPH/NADP+ of the gvp40587-overexpressed strain increased 1.4 and 0.4 times than those of the control strain, respectively. These results showed that the overexpression of endogenous gvp gene cluster could improve the trypsin synthesis by S. mobaraensis, and the increases in energy metabolism and protein precursor synthesis may be the main reason for the increased yield. This is the first report regarding the important role of gvp gene in the enzyme production in S. mobaraensis, providing new ideas for the regulation of protein expression in Streptomyces.
  • SHEN Jiaying, LI Youran, SHI Guiyang
    Food and Fermentation Industries. 2023, 49(14): 9-15. https://doi.org/10.13995/j.cnki.11-1802/ts.033358
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The (R)-citramalate is a chemical precursor for synthesis of methacrylic acid (MAA), which is used in food processing, medical device, construction, lighting, and the home appliance industries. At present, (R)-citramalate is mainly produced by chemical method, and its biological synthesis technology is still in the research stage. In this study, Bacillus licheniformis was used as the chassis cell to successfully construct the (R)-citramalate production pathway for the first time, the heterologous expression of citramalate synthase was realized in the recombinant B. licheniformis and the glucose was used as the substrate to achieve the synthesis and accumulation of (R)-citramalate. Subsequently, the conditions for whole-cell biocatalysis were optimized. It was found that temperature and time could significantly affect the yield of (R)-citramalate. Finally, whole-cell catalysts were prepared in CMC medium, the yield of (R)-citramalate was achieved at 8.57 g/L and the conversion rate of it achieved at 142.84 mg/g glucose after a reaction time of 120 h, by controlling the transformation condition at a pH of 7.0, temperature of 37 ℃, rotation speed of 250 r/min, substrate concentration of 100 g/L and OD600 of 70. To the best of our knowledge, the titer of (R)-citramalate is the highest reported to date in shake flask culture, which provided a reference for further research on efficient biosynthesis of (R)-citramalate.
  • DU Yang, HUA Tianyu, XIAO Zhuangbo, QU Yunhe, ZHAO Mingxing, RUAN Wenquan, SHI Wansheng
    Food and Fermentation Industries. 2023, 49(14): 16-24. https://doi.org/10.13995/j.cnki.11-1802/ts.035389
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Wastewater with high content of protein usually produced in many factories for food processing. For treating this kind of wastewater, anaerobic digestion could be an efficient and cost-effective method, and resource recovery can be attained. However, the activity of the methanogens could be inhibited by the produced ammonia that converted from the contained protein, and result in lower anaerobic digestion performance. In this study, a membrane contactor for ammonia removal was applied for ammonia recovery during the anaerobic digestion process of wastewater with high content of protein, aiming at alleviating the inhibitory effect of ammonia and at the same time recovering ammonia from wastewater. The results showed that, compared with the control group, the ammonia maintained at a low level (lower than 2 000 mg/L) in the experimental reactor with ammonia recovery, the cumulative methane production increased by 54%, and the COD removal rate increased by 88%. Hydrogentrophic methanogens were dominant during the anaerobic digestion of wastewater with high content of protein, and a large number of protein and acid degrading bacteria enriched in the experimental reactor after ammonia recovery. It was beneficial for the conversion of organic substances into acetic acid, H2 and CO2, and promoted the hydrophilic methanogenesis and direct interspecies electron transfer (DIET) efficiency. Furthermore, ammonia recovery alleviated the inhibitory effect of ammonia, promoted the activity of key enzymes, and ultimately enhanced the anaerobic digestion performance. This study could provide a promising method for anaerobic digestion of wastewater with high content of protein.
  • PENG Jiayi, LI Yao, ZHANG Mengyu, WANG Qingqing, ZHONG Qingping
    Food and Fermentation Industries. 2023, 49(14): 25-34. https://doi.org/10.13995/j.cnki.11-1802/ts.033241
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    A space mutation strain of Leuconostoc enterica L21-49 that is of high-yield of exopolysaccharide (EPS) was token as the research object, its EPSs was extracted and purified by sequential column chromatography on DEAE-Sepharose Fast Flow and Sephadex G-200. The molecular mass and the structure of the purified EPSs were determined by gel permeation chromatography, infrared spectroscopy and liquid chromatography. Meanwhile, the anti-biofilm, anti-oxidation, and the inhibitory activities on α-amylase and α-glucosidase were analyzed. The results showed that EPS-A, EPS-B, and EPS-C were purified from the exopolysaccharides produced by L21-49, with the relative molecular weights of 60 629, 36 959 and 24 714 Da, respectively. EPS-A mainly contained mannose and glucose, EPS-B mainly contained mannose and galactose, and EPS-C mainly contained glucose and galactose. In vitro functional activity studies showed that the purified EPSs exhibited good inhibitory activities against pathogenic biofilms. The antioxidant capacity and inhibitory activities on α-amylase and α-glucosidase of EPS-C were higher than those of EPS-A and EPS-B, it might be related to its molecular weight and functional groups. The findings might be of positive significance for the development and utilization of the EPSs of lactic acid bacteria.
  • LU Zhihao, ZHAO Xiaohan, PEI Chenhao, FENG Xiaowen, CHENG Qingli, GU Ruizeng, LI Guoming, LIU Wenying
    Food and Fermentation Industries. 2023, 49(14): 35-40. https://doi.org/10.13995/j.cnki.11-1802/ts.026488
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The antioxidant effect and antiallergic activity of corn oligopeptides, wheat oligopeptides, calcium-chelating corn oligopeptides, and ferrum-chelating wheat oligopeptides were studied based on the physicochemical property of two oligopeptides. The ability to scavenge DPPH radical and hydroxyl radical as well as the reductive power and hyaluronidase activity inhibition were analyzed. The antioxidant capacity analysis showed that the four samples had the strong antioxidant capacity, and the hydroxyl radical scavenging ratio of the two chelates was significantly higher than that of the corresponding oligopeptides. The analysis of antiallergic activity indicated that the hyaluronidase inhibition ratio of the chelates was much higher than that of the corresponding oligopeptides when the concentration was less than 20 mg/mL. There was a certain positive correlation between the antioxidant capacity and antiallergic activity of the sample because of the similarity between the results of hydroxyl radical test and the hyaluronidase inhibition test. However, the inhibition ratio of oligopeptides increased significantly at a concentration of 20-100 mg/mL, while the inhibition ratio of chelates tended to be flat. The chelates possess both strong antioxidant capacity and antiallergic activity when the concentration was less than 20 mg/mL, so the chelates can be further applied and developed in the food and medicine area.
  • WANG Xiaoyu, LI Tong, CHANG Chenguang, REN Guiping, YANG Haiyan, WU Yun, HUANG Wenshu
    Food and Fermentation Industries. 2023, 49(14): 41-49. https://doi.org/10.13995/j.cnki.11-1802/ts.032140
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The epidermis of Lycium barbarum is attached with a thick waxy layer, which makes the drying speed slow. During drying, due to the influence of heat and oxygen for a long time, it will undergo a browning reaction and cause color deterioration, the drying speed and appearance quality of Lycium barbarum should be improved by using a drying-promoting and color-protecting agent. This paper aimed to explore the mechanism of the drying-promoting and color-protecting agent in the drying process of wolfberry. In this study, taking the non-sulfur drying promoting and color-protecting agent as the treatment group and the untreated wolfberry as the control, the related indexes of enzymatic browning and non-enzymatic browning were measured during the whole drying process and the microstructure of the epidermis was observed. Results showed that the enzymatic browning of Lycium barbarum was dominant in the early drying stage and the non-enzymatic browning was dominant in the later drying stage. The drying-promoting and color-protecting agent could inhibit the activities of polyphenol oxidase (PPO) and peroxidase (POD) during the drying process of wolfberry and the accumulation of 5-hydroxymethylfurfural (5-HMF), maintain a high reducing sugar and free amino acid content, and had a strong inhibitory effect on the enzymatic browning and carbonyl ammonia reaction browning of Lycium barbarum. After the microstructure observation, it was found that the drying-promoting and color-protecting agent destroyed the waxy layer structure of the epidermis of wolfberry, which could promote the penetration of the color-retaining agent while increasing the drying speed. Through the measurement and analysis of each index and the observation of microstructure, the type of browning inhibition, and the mechanism of promoting drying and color protection of Lycium barbarum can be judged, which provides a theoretical basis for effectively controlling browning and improving the overall quality of Lycium barbarum.
  • PU Tianting, LAN Weiqing, ZHU Shengyun, ZHAO Xinyu, XU Zhenfei, XIE Jing
    Food and Fermentation Industries. 2023, 49(14): 50-59. https://doi.org/10.13995/j.cnki.11-1802/ts.032468
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To study the effect of sodium lactate combined with osmotic technology on quality changes of large yellow croaker (Pseudosciaena crocea) during refrigerated storage, samples were treated with head, tail, and viscera removed, washed with distilled water, and drained, then they were divided into 4 groups randomly. Samples were treated by 20/28 kHz, 175 W dual-frequency ultrasound (DUS), 3% sodium lactate (SL), and sodium lactate combined with dual-frequency ultrasound (SLUS) for 10 min, respectively, samples treated with sterile distilled water for 10 min were used as the control group (control, CK). Then they were drained, put into sterile blender bags, and stored at 4 ℃. Different parameters, such as microbial indexes , physicochemical indexes , texture profile analysis (TPA), water migration , protein characteristics , and sensory evaluation were measured every 2 days. Results showed that DUS treatment could properly inhibit the growth of microorganisms at the beginning of storage, the TVC of CK and DUS groups at day 0 were 4.31±0.02 and 3.22±0.09, respectively, which were significantly different from each other (P<0.05). The shelf-life of Pseudosciaena crocea with dual-frequency ultrasound treatment during refrigerated storage was 8 days. SL treatment could improve the WHC and sensory scores of samples. The shelf-life of the large yellow croaker was prolonged to 12 days during the whole storage period. Meanwhile, the WHC of the SL group was significantly higher than those of the CK and DUS groups (P<0.05). SLUS treatment could slow down the growth of TVC and PBC effectively, the increase of pH, TVB-N, and TBA value was inhibited, and its good texture characteristics and quality were maintained at the end of storage. The results of WHC, LF-NMR, and MRI analysis showed that SLUS treatment postponed the migration of immobilized water to free water, indicating that SLUS treatment could greatly maintain the water content and slow down the rate of water loss. The TVB-N, TBA, hardness, springiness, and chewiness of the SLUS group were significantly different from those of the CK group since the 2nd day (P<0.05). From the results of IFI and SDS-PAGE analysis, SLUS treatment could effectively reduce the rate of protein oxidation. Based on the analysis of various parameters, it was concluded that SLUS treatment could prolong the shelf-life of Pseudosciaena crocea for another 6 days when compared with the CK group. In conclusion, dual-frequency ultrasound-assisted sodium lactate treatment could significantly extend the shelf-life of Pseudosciaena crocea and effectively maintained the sensory quality. Results could provide a theoretical reference for the preservation of Pseudosciaena crocea during refrigerated storage.
  • MO Yi, RAN Xiaoqin, WANG Zancheng, CHEN Yudian, PENG Nian, LI Jianghong, LI Yonghao
    Food and Fermentation Industries. 2023, 49(14): 60-66. https://doi.org/10.13995/j.cnki.11-1802/ts.033370
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    A mixture of glucose and sophorose (MGD) as developed from glucose through the transglycosylation reaction catalyzed by β-glucosidase, resulting in the overproduction of cellulase by Trichodema reesei Rut C30. However, proteomics showed that the significantly low expression of the SWO1 gene resulted in a low efficiency of cellulase hydrolysis compared with that of cellulose as an inducer. Therefore, the endogenous Trswo1 gene was overexpressed in T. reesei Rut C30 using a constitutive promoter pyruvate decarboxylase 1(PDC1), and the SWO1 transcription levels of the two transformants T. reesei OEswo1-1 and OEswo1-2 were increased by 4.65 times and 3.91 times, respectively. There was no significant change in filter paper activity, endoglucanases, or cellobiohydrolases, but the crude enzymes of T. reesei OEswo1-1 and OEswo1-2 were applied to hydrolyze corn stover with a solid loading of 5%, and the hydrolysis efficiencies were increased by 6.98% and 13.93%, respectively. Furthermore, the X-ray diffraction (XRD) and the scanning electron microscope demonstrated that the enhanced hydrolysis efficiency was primarily triggered by the decrease in the crystallinity of lignocellulose. Furthermore, the fiber structure exhibited varying degrees of loosening and disintegration. The results of this study have implications for the low content of auxiliary protein that degrade cellulose in cellulase systems synthesized by T. reesei with MGD as an inducer.
  • XUE Siya, ZHANG Runfeng, WANG Qing, CHEN Shan
    Food and Fermentation Industries. 2023, 49(14): 67-72. https://doi.org/10.13995/j.cnki.11-1802/ts.033074
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    This research used ultrasonic-assisted technique to prepare thyme essential oil nanoemulsion with different concentrations and droplet sizes, then explored the inhibitory effects of concentration and droplet size of nanoemulsion on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. It was observed by transmission electron microscopy that the nanoemulsions were perfectly spherical with the average droplet size of 50 nm, and were comparable to the results measured by particle size analyzer. Fourier transform infrared spectroscopy showed that the change of ultrasonic power could adjust the droplet size of the nanoemulsion but had no effect on the chemical structure of its components. Antimicrobial activity research proved that the concentration of thyme essential oil nanoemulsion had a significant effect on the antibacterial of different strains (P<0.05), while the droplet size had difference on antibacterial effect of different strains. P. aeruginosa had the most significant effect (P<0.05), while that of E. coli was not as apparent as that of P. aeruginosa. The results showed that the optimal combination of the concentration and droplet size of the thyme essential oil nanoemulsion could enhance its antibacterial effect.
  • WANG Yuting, AN Kejing, XU Yujuan, PENG Jian, YU Yuanshan, WU Jijun, XIAO Gengsheng
    Food and Fermentation Industries. 2023, 49(14): 73-79. https://doi.org/10.13995/j.cnki.11-1802/ts.032074
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To study the effect of different desugaring pretreatment on litchi fruit powder, the effects of washing and yeast fermentation on the desugaring effect, dispersion characteristics, and nutritional quality of litchi fruit powder were studied. Results showed that the total sugar content of the untreated fruit powder was 155.61 mg/g and the desugaring rates of the fermentation and washing treatment were 84.02% and 71.59%, respectively. Compared with the untreated fruit powder, the bulk density, slide angle, and the angle of repose of litchi powder after washing and fermentation pretreatment decreased, and the bulk density, slide angle, and the angle of repose of litchi powder after fermentation treatment were lower than those of the washed fruit powder, indicating that the fermented litchi fruit powder had better mobility. The total phenolic content of fermented litchi fruit powder was 20.70 mg/g, followed by 19.82 mg/g in untreated fruit powder and 17.66 mg/g in water-washed fruit powder. DPPH radical and ABTS cationic radical scavenging activities were similar to the trend of total phenolic content, indicating that fermentation desugaring pretreatment better preserved the nutritional function characteristics of fruit powder. Scanning electron microscope results showed that the untreated fruit powder was uneven in size and sticky. The fermented fruit powder was smooth and wrinkled and the size of the particles was more uniform as the crushing time increased. However, the surface of the fruit powder pretreated by water washing was not smooth and had shrinkage, and with the increase in crushing time, the fruit powder appeared agglomeration. Therefore, the fermented desugaring treatment had better applications than the water-washing treatment.
  • LI Yunchang, WANG Longqiong, HE Xiaoling, DING Desheng, WANG Yuankai, ZHOU Fenglan, CAI Tian, CHEN Kewei
    Food and Fermentation Industries. 2023, 49(14): 80-87. https://doi.org/10.13995/j.cnki.11-1802/ts.033086
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Vitamin A and its precursors in breast milk play an important role in the growth and development of infants and young children. In this study, the qualitative and quantitative analyses of β-carotene, vitamin A, and vitamin A esters in breast milk under different lactation stages were performed with ultra-high performance liquid chromatography/quadrupole time of flight mass spectrometry. Their intrinsic patterns of distribution in breast milk were investigated by variance analysis, principal component analysis, correlation analysis, and cluster heat map analysis. Results showed that the contents of β-carotene and vitamin A in breast milk gradually decreased with the extension of the lactation period, and the two contents were correlated (Pearson correlation coefficient was 0.416). The content of vitamin A esters in breast milk was relatively stable, and a total of nine vitamin A esters were detected under the three lactation stages, with long-chain retinyl esters being the main form present and retinyl behenate having the highest content. There was a certain clustering among different vitamin A esters, and the metabolic correlation between retinyl caprylate, retinyl docosanoate, and retinyl docosahexanoate was the strongest (Pearson correlation coefficient was 0.91). The results of this study can provide a reference for formula research and the development of infant formula food.
  • SU Hongxia, GAO Jing, JIANG Min, JIA Yao
    Food and Fermentation Industries. 2023, 49(14): 88-93. https://doi.org/10.13995/j.cnki.11-1802/ts.033408
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Cyclocarya paliurus is a kind of precious homologous plant for medicine and food. Polysaccharide from C. paliurus CP50 is the pectinic acidic polysaccharide with strong biological activity. Xanthine oxidase (XO) is a purine catabolism enzyme associated with hyperuricemia and gout. In this study, according to the orthogonal experiments, the extraction condition parameters of the polysaccharide CP50 from the dried leaves of C. paliurus were optimized as follows: extraction temperature 100 ℃, solid-liquid ratio 1∶40(g∶mL), extraction time 2 h, and the polysaccharide yield was 1.67%. The inhibition mechanism of CP50 was explored by using XO as the target and combined with molecular simulation methods. The results showed that CP50(0-1 mg/mL) haD a certain inhibitory effect on XO, and its inhibitory activity was concentration-dependent, with IC50 of 0.825 mg/mL. The molecular docking results showed that, CP50 molecules were inserted into the hydrophobic cavity of XO and occupied the catalytic center, bringing the inhibition activity of XO and the effects of reducing uric acid. The purpose of this study is to provide method reference and data support for the application of CP50 as a useful dietary polysaccharide in daily diet of patients with hyperuricemia.
  • LI Liuruolan, ZHANG Chengcheng, LIU Bingshu, YU Leilei, TIAN Fengwei, CHEN Wei, ZHAI Qixiao
    Food and Fermentation Industries. 2023, 49(14): 94-102. https://doi.org/10.13995/j.cnki.11-1802/ts.035507
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The utilization of carbohydrates by Bifidobacterium has always been a research focus, but the overall study on the utilization ability of carbohydrates by Bifidobacterium pseudocatenulatum is still lacking. In order to deepen the understanding of carbohydrate utilization in B. pseudocatenulatum, the growth curve and in vitro fermentation experiments were used to study the utilization ability of 12 strains of B. pseudocatenulatum to 17 carbohydrates. B. pseudocatenulatum can achieve high biomass in glucose, lactose, galactooligosaccharides (GOS) medium (OD600≥0.99), and can reach a high growth rate (generation time≤150 min) in sucrose and GOS medium. The study also revealed significant inter-strain differences in carbohydrate utilization among the 12 strains, with FJSNT36M3 utilizing only five carbohydrates, while three strains (FHNBA14M1, FGZ16I1M1, FXJWS49M21) utilize more than 10 carbohydrates. On the generation time and OD600 indicator, three strains (FGSYC12M4, FGZ16I1M1, FFJND17M1) has a strong ability to utilize nine kinds of carbohydrates. Most of the glycoside hydrolases encoded by the 12 strains of B. pseudocatenulatum degrade plant-derived carbohydrates. The differences in carbohydrate utilization among strains may due to the differences in the enzymes that degrade and transport carbohydrates. In vitro fermentation experiments of human feces proves that the difference in carbohydrate utilization ability of B. pseudocatenulatum relates to its potential to occupy abundance in gut.
  • HU Yunfeng, CHEN Yanan, CHEN Junran, HU Ying
    Food and Fermentation Industries. 2023, 49(14): 103-109. https://doi.org/10.13995/j.cnki.11-1802/ts.032184
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    This paper aimed to study the quality changes of astaxanthin eggs with pullulan coating during storage and establish a dynamic model of egg quality decay. In the experiment, the pullulan-coated astaxanthin eggs were stored at temperatures of 15 ℃, 25 ℃, and 35 ℃, and the changes in quality indicators such as egg mass, Hough unit, air chamber diameter, and astaxanthin content were measured. The obtained data were fitted, and a quality decay dynamic model was established. Results showed that the zero-order kinetic model was suitable for fitting the changes in air chamber diameter, egg yolk coefficient, egg white pH, concentrated protein ratio, and astaxanthin content, and the first-order kinetic model was suitable for fitting the mass index, and the mean R2 was greater than 0.95. The correlation between astaxanthin content and Hough unit was strong, and R2 was 0.976. The relative error of the shelf-life prediction model of pullulan-coated eggs with high astaxanthin content was less than 10% based on the astaxanthin content. It indicated that the model could well predict the remaining shelf life of pullulan-coated eggs with high astaxanthin content stored at 15-35 ℃.
  • SHEN Yuping, HE Xi, ZHOU Ziwei, HE Chunlan, ZHANG Zujiao
    Food and Fermentation Industries. 2023, 49(14): 110-117. https://doi.org/10.13995/j.cnki.11-1802/ts.035417
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Salidroside is a high value-added natural product with great potential of application and development. However, the current de novo synthesis of salidroside in Escherichia coli suffers from poor yield and high proportion of intermediate tyrosol. Combination of the phenylpyruvate decarboxylase mutant ARO10F138L//D218G obtained in our previous study, and screening of the key enzymes of alcohol dehydrogenase and uridine diphosphate glucose glycosyltransferase in salidroside synthesis pathway, the titer of salidroside reached up to (605.8±16.8) mg/L. Furthermore, the titer of salidroside was increased by 95.0% to (1 156.3±44.2) mg/L with deletion of feaB and overexpression of pgm and galU for enhancing supply of uridine diphosphate glucose. To overcome the problems of high accumulation of intermediate tyrosol and low cell density in salidroside fermentation, a quorum-sensing circuit was used to dynamically control the salidroside synthesis pathway, which alleviated the toxic effect of tyrosol on chassis cells and lowered the proportion of tyrosol successfully. To this end, the titer of salidroside was increased by 36.3% to (1 484.6±21.4) mg/L, the OD600 increased by 31.6 % to 8.18±0.23. In a 2 L fermenter, the production of salidroside in the engineered strain dynamically regulated by quorum-sensing reached 4.38 g/L with an OD600 of 46.74. Compared with those in the engineered strain statically regulated by induced expression, the yield of salidroside was increased by 48.2%, the OD600 was enhanced by 26.6%, and the proportion of tyrosol in fermentation broth was also reduced from 33.7% to 17.9%, which showed great potential for production of salidroside. In the current study, the results revealed that dynamic quorum-sensing regulation could effectively reduce the accumulation of intermediates and alleviate the inhibitory effect of metabolite toxicity on chassis cells. This study has important reference significance and great application value for the biosynthesis of other glycoside natural products or chemicals with high toxicity.
  • DANG Wenqian, LIU Mei, WU Xiaonan, MA Hao, SUN Le, LI Jie, ZHENG Xueling, LI Limin, LIU Chong
    Food and Fermentation Industries. 2023, 49(14): 118-125. https://doi.org/10.13995/j.cnki.11-1802/ts.032118
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Nowadays, people have high requirements for the nutrition and health of food, and the fried dough sticks with high oil content do not conform to the concept of healthy life in China, so it is necessary to develop fried dough sticks with low oil content. In this study, three kinds of flour with different quality grades were selected, and yeast was used as a starter to explore the effects of the freezing storage process on oil content, specific volume, texture, and sensory evaluation of pre-fried and direct-fried quick-frozen dough sticks. After freezing and thawing, the hardness and chewiness of the two kinds of quick-frozen pre-made fried dough sticks increased, while the elasticity and resilience decreased, the specific volume decreased, and the sensory evaluation value decreased. Compared with pre-fried quick-frozen dough sticks, direct-fried quick-frozen dough sticks had lower oil content, larger specific volume, higher hardness, and slightly lower sensory evaluation value. Flour with high protein content and high wet gluten content was helpful to improve texture characteristics and sensory evaluation of fried dough sticks. Fried dough sticks made of flour with low ash content and high precision tended to have low oil content. Direct-fried quick-frozen dough sticks could greatly reduce the oil content of fried dough sticks. In this study, dry yeast fermentation was used to reduce the frying times, and the quick-frozen fried dough sticks with lower oil content were developed, which provided some ideas for the production of quick-frozen fried dough sticks.
  • CHEN Jinfeng, LI Min, CUI Yanli, ZHANG Shenggui
    Food and Fermentation Industries. 2023, 49(14): 126-131. https://doi.org/10.13995/j.cnki.11-1802/ts.033024
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    In order to explore the changes in physicochemical and structural characteristics of wheat starch during the fermentation and baking of bread processing, the high gluten wheat flour Lantian 45 from Qingshui County, Gansu Province was used for bread making in this study. Wheat starch was extracted at different stages of dough fermentation and bread baking, and its physicochemical and structural characteristics were determined and characterized. The results showed that the particle morphology and crystal type of starch remained unchanged at different stages of dough fermentation. The relative crystallinity, degree of order, and double helicity of starch molecules increased from 39.77%, 1.19, 1.14 to 47.85%, 1.28, 1.23, respectively. During the different stages of bread baking, with the extension of baking time, the starch granules gradually collapsed, and the adhesion between the granules gradually formed a sheet structure. The average volume diameter D[4,3] value of starch increased significantly from 16.49 to 22.63 μm, the relative crystallinity of starch molecules decreased from 47.85% to 25.28%, and the thermal stability of starch increased. Fourier transform infrared spectroscopy showed that the functional groups of starch molecules were not changed during fermentation and baking. This study indicated that the physicochemical and structural characteristics of wheat starch changed significantly at different stages of dough fermentation and bread baking, which in turn affected the final quality of bread.
  • WANG Jingwen, FANG Zhijia, GAO Yuan, GUAN Wenhao, SUN Lijun, LIU Ying
    Food and Fermentation Industries. 2023, 49(14): 132-137. https://doi.org/10.13995/j.cnki.11-1802/ts.032593
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To explore the protective effect and mechanism of cysteine (Cys) on cadmium-induced liver injury in mice. SPF Kunming mice were divided into control group, cadmium (Cd) group (intraperitoneal injection of cadmium chloride), cadmium and low-dose Cys co-treatment group (intraperitoneal injection of cadmium chloride plus oral administration of low-dose Cys), cadmium and high-dose Cys co-treatment group (intraperitoneal injection of cadmium chloride plus oral administration of high-dose Cys). After the treatment, the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and the contents of total cholesterol (TC) and triacylglycerol (TG) in liver tissue were determined; The pathological changes of liver tissue were observed via HE staining. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) in liver tissue were detected by microvolume assay; The levels of IL-6, IL-1β and TNF-α in liver were detected by ELISA. The expression levels of genes related to lipid metabolism in liver were measured by qRT-PCR. The results showed that the activities of serum ALT and AST were significantly increased in Cd-exposed mice compared to the control group (P<0.01). The liver tissue had obvious pathological changes and the contents of TC and TG in the liver were significantly increased (P<0.01). The level of MDA in liver tissue was significantly increased, and the activities of SOD and CAT were significantly decreased (P<0.01). The supplementation of Cys can significantly improve the adverse changes of the indicators above (P<0.05 or P<0.01). In addition, Cys supplementation enhanced anti-inflammatory potential during Cd exposure and normalized pro-inflammatory cytokine levels. Cys also normalized the expression of Cd-disturbed lipid metabolism-related genes to improve lipid accumulation, which helped reduce liver damage. The treatment of Cys reduced oxidative stress and inflammatory responses, which may help to prevent Cd-induced lipid metabolism disorders and liver injury.
  • LIU Hui, SHAN Mei, SONG Jiaqi, LI Jiaxu, WANG Hanting, LI Yongye
    Food and Fermentation Industries. 2023, 49(14): 138-146. https://doi.org/10.13995/j.cnki.11-1802/ts.032136
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The direct stacking of agro-processing by-products will cause serious environmental pollution. This study applied the single factor optimization method to clarify the potential of agro-processing by-products rice bran, rice husk, wheat bran, and distillers′ grains as water pesticide triadimefon pollution remediation agents. Results showed that rice bran had the best effect on the removal of triadimefon, and rice husk was the least suitable as a triadimefon remover. Among them, material mass and particle size were the most important factors affecting the removal rate of triadimefon. The optimal conditions for removing triadimefon from water by rice bran were particle size 60 mesh, mass 5 g, treatment temperature 40 ℃, and time 5 min. The change of material mass significantly affected the adsorption capacity of triadimefon to substances in water. Through the isothermal adsorption modeling, it was found that only the distillers′ grains group conformed to both Langmuir and Freundlich models at 30 ℃ by changing the material mass. The simulation effect of Freundlich for the other three materials was better, indicating that the adsorption process belonged to the heterogeneous adsorption of multi-molecular layers. No enantioselective removal of triadimefon was observed except for the 7 g rice bran-treated group. However, from the isothermal adsorption modeling, the four materials had higher adsorption potential for S-(+)-triadimefon. The results can provide a theoretical basis for the remediation of triadimefon pollution using low-cost agro-processing by-products.
  • SHI Zhijiao, WEI Guifang, HE Xuhua, XU Han, PENG Xiaowei, KAN Huan, LIU Yun
    Food and Fermentation Industries. 2023, 49(14): 147-153. https://doi.org/10.13995/j.cnki.11-1802/ts.032027
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The release amounts of polyphenols and flavonoids from Aronia melanocarpa leaves and their activities of antioxidant and sugar digestion enzyme inhibition were studied by simulated digestion in vitro, in which correlation was analyzed. Results showed that the release amounts of polyphenols and flavonoids in leaves at different digestive stages were as follows: oral digestive fluid > gastric digestive fluid > small intestinal digestive fluid > large intestine digestive fluid. Compared with undigested fluid, DPPH radical and ABTS cationic radical scavenging rate, ferric reducing power, α-glucosidase, and α-amylase inhibition rate of each digestive fluid were all decreased after simulated digestion in vitro, while hydroxyl radical scavenging rate increased significantly in the small intestine (P<0.001). Furthermore, the activity indexes except hydroxyl radical scavenging rate were positively correlated with the release of polyphenols and flavonoids in the correlation analysis. In conclusion, polyphenols and flavonoids were gradually released from the leaves of A. melanocarp during simulated digestion in vitro and showed high biological activity.
  • SHI Meimei, YANG Kai, LYU Pengjun, ZHANG Qisheng, CHEN Gong, WANG Yong, WANG Dongdong, YIN Liguo, WU Yalong
    Food and Fermentation Industries. 2023, 49(14): 154-160. https://doi.org/10.13995/j.cnki.11-1802/ts.033418
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Starting from Lactobacillus plantarum, Leuconostoc mesenteroides, and Weissella cibaria, using pH, total acid, volatile flavor components, and sensory evaluation as indicators, the effects of three single-bacteria and multi-bacteria inoculation on the quality of Sichuan pickles were studied. The results showed that, compared with natural fermentation, inoculation fermentation could start fermentation quickly and stabilize the quality of pickles; the acid-producing rates of three single bacteria were: L. mesenteroidesW. cibariaL. plantarum. A total of 85 volatile flavor components were detected by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), including 27 alcohols, 17 aldehydes and ketones, 16 esters, two acids, 13 hydrocarbons, two sulfur-containing compounds, five benzene rings, and three other compounds. A total of 16 important volatile components were analyzed, including dimethyl trisulfide, dimethyl disulfide, eucalyptol, linalool, α-terpineol, nonanal, octanal, n-decanal, acetoin, allyl isothiocyanate, 3-butenyl isothiocyanate ester, 3-methylthiopropyl isothiocyanate, 4-(methylthio)butyl isothiocyanate, phenylethyl acetate, D-limonene, and anethole. According to the analysis of volatile flavor components and sensory evaluation, the quality of pickles inoculated with L. plantarum and W. cibaria was the best, and the flavor components were close to natural fermentation in general. When the ratio of L. plantarumW. cibaria was 6∶4, the flavor of pickles was good and the acceptance degree was high. The research results lay a foundation for further application in multi-round fermented pickles.
  • MENG Yuwei, CHEN Weibo, ZHOU Jun, LI Xiangzhou
    Food and Fermentation Industries. 2023, 49(14): 161-168. https://doi.org/10.13995/j.cnki.11-1802/ts.032312
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The interaction of the mulberry leaf protein (MLP) with resveratrol (Res) and chlorogenic acid (Cla) was investigated by ultraviolet-visible (UV-Vis), fluorescence, and Fourier transform infrared (FT-IR) spectroscopies. The in vitro digestion and antioxidant activities of the complexes were also investigated. Results indicated that resveratrol and chlorogenic acid could quench the fluorescence of mulberry leaf protein, which was static quenching. At three different temperatures, the binding constants (KA) and site numbers (n) were approximately 104 L/mol and 1, respectively. The interaction between resveratrol, chlorogenic acid, and mulberry leaf protein was dominated by the van der Waals force and hydrogen bonds. Resveratrol and chlorogenic acid interacted with mulberry leaf protein to form new complexes and the mulberry leaf protein structure was altered. Furthermore, the hydrolysis rate was reduced after the addition of mulberry leaf protein and polyphenols, and it was difficult to hydrolyze by digestive enzymes such as pepsin and trypsin. The DPPH free radical and ABTS cationic radical scavenging abilities of the two complexes dropped but remained higher than those of the mulberry leaf protein itself. Resveratrol and chlorogenic acid were bound with mulberry leaf protein at varying degrees, affecting its in vitro digestion and antioxidant abilities. These findings provide a theoretical foundation for the development and application of mulberry leaf functional products.
  • ZHANG Suqi, YUN Jinhu, ZHAO Pupu, DI Changyi, WEI Ziqiu, ZHANG Wangxiang
    Food and Fermentation Industries. 2023, 49(14): 169-177. https://doi.org/10.13995/j.cnki.11-1802/ts.032371
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To explore the quality difference and the value of crabapple leaf black tea in different varieties and different periods, the study made crabapple black tea from tender raw materials of seven crabapple varieties in April, June, and August, analyzed the differences of their main components, antioxidant activity, and sensory scores, and then integrated the results of principal component analysis (PCA) and sensory score evaluation to obtain the better crabapple variety tea and period. Results showed that ‘King Arthur’ and ‘Sparkler’ had the highest contents of soluble sugar and tannic acid in June, and ‘Sparkler’ had the highest contents of free amino acids, polyphenols, total flavonoids, and procyanidins in April. The highest scavenging rates of DPPH free radicals, hydroxyl radicals, nitrites, and ferric reducing power were ‘King Arthur’ in June, ‘John Downie’ in August, and ‘Sparkler’in April and June, respectively. Phenolic substances were mainly phlorizin, and ‘John Downie’ had the highest content of phlorizin in June. The top three varieties with sensory scores were ‘Pink Ballet’, ‘John Downie’, and ‘Sparkler’, with the highest sensory scores in April. The top three varieties in the principal component analysis (PCA) composite score were ‘Sparkler’, ‘Pink Ballet’, and ‘John Downie’, with the highest overall score in April. Therefore, the crabapple varieties with excellent quality in black tea were ‘Sparkler’, ‘Pink Ballet’, and ‘John Downie’, and the picking period was the best in April.
  • LIN Yi, LAI Jiaqi, XU Huiqing, GAO Zhiheng, LIN Jingyu, LI Yun
    Food and Fermentation Industries. 2023, 49(14): 178-185. https://doi.org/10.13995/j.cnki.11-1802/ts.033356
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Staphylococcus aureus contamination is a risk factor for food safety in quick-frozen dumpling products. Biofilms represent a potential source of bacterial contamination in food processing. In order to study biofilm formation characteristics of S. aureus in quick-frozen dumpling products and the resistance to disinfectants, the investigations were carried out using strains isolated from the products. Biofilm forming ability of the isolates were investigated including biofilm formation on the surface of polystyrene (PS) and stainless steel (SS), presence of adhesion genes, effects of environmental conditions, and bactericidal effects of three disinfectants against biofilm cells. The results showed that the isolates formed stronger biofilms on the surface of SS than PS. More than four adhesion genes were detected in each isolate. The presence rate of eno and clfB genes were 100%, whereas bap gene was not detected in any of the isolates. On the surface of SS, the presence of sasG showed highly significant positive correlation with biofilm formation (P<0.01), and clfA and fnbA showed significant positive correlation with biofilm formation respectively (P<0.05), while that of icaAD, icaBC and fib showed highly significant negative correlation (P<0.01). On the surface of PS, the presence of sasG showed a significant positive correlation with biofilm formation (P<0.05), and fib showed a significant negative correlation (P<0.05). The optimal growth temperature of 37 ℃ was favorable for the formation of more biofilms. At room temperature (25 ℃), the strains still formed relatively high biomass of biofilm. The largest amount of biofilm was observed to form at pH 7. Under weak acidic and weak alkaline conditions, the biofilm formation was less affected. At NaCl<5%, a certain stimulating effect was observed on the film formation. The biofilm greatly enhanced the resistance of the cells wrapped inside to the disinfectant tested. Among the three disinfectants tested, peracetic acid was found to be the most effective against biofilms cells. The results provide new data for the study of biofilms formed by S. aureus in quick-frozen dumplings, laying the basis for further prevention and reduction of the harm of the biofilm.
  • YU Pengxin, LIU Li, ABULIKEMU Adili, WANG Yuhe, LI Junchen, GAO Xinyue, ZHANG Wanhe, BATUER Abulikemu
    Food and Fermentation Industries. 2023, 49(14): 186-192. https://doi.org/10.13995/j.cnki.11-1802/ts.032019
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To clarify the differences in muscle quality characteristics among different breeds of southern Xinjiang multi-foetus mutton sheep, 6-month-old Hu sheep, Hotan sheep, and Pishan Red sheep were selected as the research objects, and the longissimus dorsi muscle was selected after slaughter, and the food quality, main nutritional components, amino acid, and fatty acid contents of longissimus dorsi were determined and analyzed. Results showed that the muscle pH of the three sheep was between 5.24-5.83. The color of Hu sheep was significantly higher than that of Hotan sheep and Pishan red sheep (P<0.05). Hotan sheep had the highest tenderness and had a significant difference from the other two breeds (P<0.05). The cooking loss rate was 39%-45%. Hu sheep and Pishan red sheep had a smaller cooking loss, which was significantly different from Hotan sheep (P<0.05). The water and protein contents of Hu sheep were significantly higher than those of the other two sheep (P<0.05). The fat content of Hotan sheep was significantly lower than that of Hu sheep and Pishan red sheep (P<0.01). The cholesterol content was within the range of low-cholesterol foods specified in nutrition and was lower than 100 mg/100 g. A total of 16 different amino acids were detected in muscle. However, the percentage of essential amino acids/total amino acids and the percentage of essential amino acids/non-essential amino acids were slightly lower than the recommended values of FAO/WHO. The content of amino acids in Hu sheep was the most abundant, except valine, other scores were higher than FAO/WHO recommended values. A total of 33 kinds of fatty acids were detected, including palmitic acid, trans oleic acid and myristic acid. The proportion of unsaturated fatty acids in Lake mutton was the highest, which was significantly higher than that of the other two varieties (P<0.05), and the ratio of saturated fatty acids/unsaturated fatty acids (SFA/UFA) was closer to the recommended value of 1∶1. In conclusion, Hu sheep shows good quality in edible quality, amino acids, and fatty acids.
  • LIU Hongji, YU Yougui, WU Qiang, ZHANG Xu, WAN Yong, XIONG Xiang, YANG Zhilong, TAN Wenjun
    Food and Fermentation Industries. 2023, 49(14): 193-198. https://doi.org/10.13995/j.cnki.11-1802/ts.035367
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To investigate the structural characteristics of the dominant bacterial community and its correlation with the main differential metabolites in sauce-flavor Baijiu, 16S rRNA and GC-MS non-targeted metabolomics combined with multivariate statistical analysis were used to determine and compare the bacterial composition and differential metabolites among 30 fermented grains collected from different fermentation cycles. Results showed that 74 differential metabolites among them were screed out. They were classified as organic oxygen compounds, carboxylic acids and derivatives, and fatty acyl compounds. Additionally, five of the eight dominant bacterial genera, particularly such as Lactobacillus, Kroppenstedtia, and Sphingomonas, played a synergistic role in key metabolic pathways including ABC transport protein metabolism, starch and sucrose metabolism, pentose phosphate pathway, and the mutual transformation of pentose and glucose esters. This study can clarify the community composition of dominant microorganisms sauce-flavor Baijiu collected from different fermentation cycle and clear their interrelationship with the major differential metabolites, which provide a theoretical basis for artificially improving the fermentation microecology and targeting characteristic metabolite production during sauce-flavor Baijiu brewing.
  • WU Yongxiang, QIN Mengsi, HUA Chun, LI Chen, WANG Faqian, GUO Xin, WAN Zhibing, SHE Xinsong
    Food and Fermentation Industries. 2023, 49(14): 199-206. https://doi.org/10.13995/j.cnki.11-1802/ts.032178
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Polygonati rhizoma tea was prepared by two different processing techniques of hot air drying and baking, and the texture properties, volatile components, sensory evaluation, and antioxidant activities were compared and analyzed. It was found that the different processing techniques had a great effect on the color parameters but had no significant effect on the rehydration rates, shrinkage rates, and microstructure. The results of solid-phase microextraction combined with gas chromatography-mass spectrometry (SPME/GC-MS) analysis showed that the main volatile components of dried and baked Polygonati rhizoma tea were similar but different in contents. The volatile components were mainly alkanes and aromatic compounds, and phenethyl alcohol, methyl eugenol, and eucalyptol might be the characteristic flavor substances. The sensory quality, total polyphenols, flavonoid contents, and ABTS cationic radical and DPPH free radical scavenging activities of Polygonati rhizoma tea processed by hot air drying were relatively better. This research can provide a theoretical basis for the effective development and utilization of Polygonati rhizoma.
  • LUO Guoliu, SUN Yan, ZHONG Jinfeng, LIU Xiong, QIN Xiaoli
    Food and Fermentation Industries. 2023, 49(14): 207-213. https://doi.org/10.13995/j.cnki.11-1802/ts.032433
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Moringa oleifera seed oil is prone to oxidation and degradation at high temperature during processing, which reduces its quality. Therefore, it is important to investigate the thermal stability of Moringa oleifera seed oil for expanding the use of the oil. Palm oil was used as a reference to study the thermal stability of Moringa oleifera seed oil extracted by aqueous enzymatic extraction. With increasing heating temperature and heating time, the total oxidation value and an acid value of Moringa oleifera seed oil and palm oil were increased, while tocopherol content was decreased. After heating at 200 °C, the total oxidation values of Moringa oleifera seed oil and palm oil increased by 2.1 meq/kg and 12.0 meq/kg, respectively, the acid values of Moringa oleifera seed oil and palm oil were 1.1 times and 1.3 times of their initial acid values, respectively, and the retention of α-tocopherol in Moringa oleifera seed oil was 1.2 times of that in palm oil. When heating after 120 min, the acid value of Moringa oleifera seed oil and palm oil increased by 23.8% and 52.6%, respectively, and the retention of α-tocopherol in Moringa oleifera seed oil was 12.4% higher than that of palm oil. Thermogravimetric analysis showed that the initial decomposition temperature of Moringa oleifera seed oil was 15.9 ℃ higher than that of palm oil, and the mass loss of Moringa oleifera seed oil was 3.1% lower than that of palm oil. Therefore, Moringa oleifera seed oil has better thermal stability than palm oil and has significant potential in frying oil.
  • YANG Feng, HU Chenggang, YAN Ling, LI Xianzhi, CHEN Yanhe, HU Yang
    Food and Fermentation Industries. 2023, 49(14): 214-220. https://doi.org/10.13995/j.cnki.11-1802/ts.032379
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    In recent years, consumers are paying more and more attention to health, and the demand for health care products is also increasing. Health wine, as a kind of health care product, is more and more favored by consumers, but at the same time, most consumers lack accurate understanding of the efficacy of some health wine. In order to meet the needs of consumers, increase consumers′ awareness of health wine, guide their healthy and reasonable consumption, and promote the high-quality development of health wine. This study took Zhuifenbazhen wine as the object, studied the trace components in Zhuifengbazhen wine, and analyzed its efficacy, so as to provide a scientific basis for the function of Zhuifengbazhen wine and increase consumers′ understanding of health wine, guiding their scientific and reasonable consumption.
  • MA Chenyang, HUANG Pimiao, DUAN Ping, WANG Lu, FENG Xiya, KAN Jianquan
    Food and Fermentation Industries. 2023, 49(14): 221-228. https://doi.org/10.13995/j.cnki.11-1802/ts.032179
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    This study aimed to explore the active ingredients of the rosemary perfume extract to remove the fishy smell of silver carp meat. After removing the polyphenols, polysaccharides and proteins from the extract of rosemary perfume, the experiment was carried out to soak the deodorant of the silver carp meat. The gas chromatography-mass spectrometry (GC-MS) was used to detect the content of volatile components. To determine the main deodorizing components of rosemary perfume extract, the changes in volatile components and sensory odor value were taken as indicators. Qualitative and quantitative analysis of phenolic substances in rosemary perfume extract was performed by liquid chromatography-mass spectrometry (LC-MS/MS), and deodorization experiments with different concentrations of phenolic monomers were carried out. Results showed that compared with the treatment group without rosemary perfume extract, the smell value and volatile matter content of the silver carp meat significantly reduced after using rosemary extract. The highest fishy smell value dropped by about 57% and the volatile component content decreased by about 40%-45%. There was no significant difference in the deodorization effect between the protein group and polysaccharide group and the control group, and the lower the content of polyphenols in the polyphenol group, the worse the deodorization effect. Four kinds of phenolic compounds were detected in the extract of rosemary perfume. Rosmarinic acid (RA) was selected for the deodorization experiment. The deodorization effect of RA on silver carp meat was positively correlated with its concentration. The above results showed that polyphenols were the main deodorant components of rosemary perfume extract, and RA was the main deodorant active substance of rosemary perfume extract.
  • WANG Yurong, GE Dongying, CAI Wenchao, LIU Zhongjun, SONG Ziyun, GUO Qianzhang, GUO Zhuang
    Food and Fermentation Industries. 2023, 49(14): 229-234. https://doi.org/10.13995/j.cnki.11-1802/ts.033531
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    In this study, the fungal flora structure of low-temperature Daqu in Changzhi area was firstly analyzed by high-throughput sequencing technology, and then the flavor quality was evaluated by electronic nose, finally, the correlation between fungal flora and flavor quality was analyzed. High-throughput sequencing showed that the fungal genera present in all samples with an average relative content greater than 1.0% were Rhizopus, Aspergillus, Dipodascus, Hyphopichia, Lichtheimia, Rhizomucor, and Saccharomycopsis. It was found by electronic nose detection that the volatile substances in low-temperature Daqu were mainly organic sulfides, terpenoids, and ethanol. The correlation analysis showed that Lichtheimia was significantly positively correlated with Rhizomucor (P<0.05) and Hyphopichia was significantly positively correlated with Saccharomycopsis (P<0.05). As for fungal genera and flavor quality, Rhizomucor showed a significant positive correlation with organic sulfide (P<0.05), and Lichtheimia showed a significant positive correlation with aromatic substances (P<0.05). Thus, it can be seen that low-temperature Daqu in Changzhi area was rich in fungal taxa and there was a correlation between fungal genera and flavor quality.
  • CHEN Xiaoqin, MU Yingchun, SU Wei
    Food and Fermentation Industries. 2023, 49(14): 235-241. https://doi.org/10.13995/j.cnki.11-1802/ts.033657
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Dendrobium Xiaoqu was used as starter to study the flavor changes of glutinous rice beverage during fermentation. The physicochemical indexes, organic acids and volatile flavors in different fermentation stages (0, 12, 24, 36, and 48 h) by chemical methods, HPLC, headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MC) combined with orthogonal partial least squares discriminant analysis (OPLS-DA) and odor activity value (OAV). The results showed that the pH and starch content of fermented grains decreased significantly (P<0.05); total acid, amino acid nitrogen, reducing sugar and sugar content were opposite. Seven kinds of organic acids were found during fermentation, and the total content increased significantly (36.16-422.4 mg/L); lactic acid, succinic acid, and citric acid were the main organic acids; oxalic acid and acetic acid were added after fermentation. HS-SPME-GC-MS showed that 58 volatile flavor compounds were detected during fermentation, mainly alcohols and esters; 33 flavor compounds screened by OPLS-DA had significant differences during fermentation; by calculating OAV, it was found that 1-octene-3-ol, phenylethanol and nonaldehyde were the characteristic aroma compounds of glutinous rice beverage. This paper provides a reference value for the study of the flavor of glutinous rice beverage, and also provides a new research direction for the further processing and expanding application of dendrobium.
  • CHEN Min, YIN Mingyu, ZHAO Yimeng, WANG Xichang
    Food and Fermentation Industries. 2023, 49(14): 242-249. https://doi.org/10.13995/j.cnki.11-1802/ts.033208
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To investigate the effects of stevia extract on lipid metabolism, oxidative stress and intestinal microecology. We chose healthy Kunming mice, built models of lipid metabolic disorders, and examined changes in blood lipids, oxidative stress, and intestinal flora. Stevia extract significantly reduced serum lipids indices (triglycerides, cholesterol, low-density lipoprotein, and free fatty acids), considerably increased serum levels of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), and decreased aldehyde content. Histopathological observation showed that stevia extract significantly reduced liver and colon tissue damage, such as data from high-throughput sequencing showed the stevia extract lowered the number of Firmicutes and raised the number of Bacteroidetes in the intestine. Stevia extract has prebiotic effect and can control lipid metabolism from anomalies, oxidative stress from out-of-balance, and liver fatty deposition.
  • ZHANG Qiaozhen, DING Yanan, GU Fengying, GUO Qin, QU Yang, LI Tian, WANG Feng, WANG Qiang
    Food and Fermentation Industries. 2023, 49(14): 250-256. https://doi.org/10.13995/j.cnki.11-1802/ts.033385
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Lactic acid preservation is a commonly used antibacterial process in the industrial processing of instant wet noodles. Acid-reducing leads to the growth of microorganisms. In order to identify the dominant spoilage microorganisms and their growth characteristics in slightly acidified preserved instant wet noodles using lactic acid, slightly acidified and acidified preserved instant wet noodles were used as the research objects to analyze the microbial diversity in this study. At the same time, combining with the traditional separation and culture methods, the dominant spoilage bacteria in lactic acid preserved instant wet noodles were obtained through morphological and molecular biological identification. The growth characteristics of dominant spoilage bacteria were also studied. The microbial diversity analysis showed that the dominant spoilage microorganisms in slightly acidified preserved instant wet noodles were Bacillus sp. Four strains of Bacillus amyloliquefaciens and one strain of Peribacillus frigoritolerans were isolated from the spoilage slightly acidified preserved instant wet noodles. B. amyloliquefaciens were the dominant spoilage bacteria in the cultivable microorganisms of slightly acidified preserved instant wet noodles. They were non-hemolytic facultative aerobic bacterium with a maximum tolerance of pH 4.70 and a maximum salt tolerance of 12%. Spores could survive after heat treatment at 100 ℃ for 5 min, with a survival rate of 84.99%-92.71%. This study provided a theoretical basis for the improvement of industrial acid reduction technology and the development of preservation technology for fresh instant wet noodles.
  • CHEN Dongjie, LI Huisheng, SUI Qing, YUN Yanling, ZHANG Yuhua, DUAN Yupeng, SUN Chongde
    Food and Fermentation Industries. 2023, 49(14): 257-264. https://doi.org/10.13995/j.cnki.11-1802/ts.035314
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To explore the differences in the quality and volatile aroma of different durian varieties during postharvest, this study collected samples of seven common species of durian on the market, namely, D13, D24, D197, D198, D201, D200, and D159, and measured their nutrient qualities such as soluble solids, vitamin C, hardness, soluble protein, and total acid. Gas chromatography on mobility spectroscopy (GC-IMS) and electronic nose technology were used to collect the odor fingerprints and volatile organic compounds (VOCs) of seven species of durian, combining analysis of variance, principal component analysis (PCA), and linear discriminant analysis (LDA). Analytical methods such as fingerprint similarity analysis (FSA) distinguished the flavor differences of seven species of durian. Results showed that there were differences in nutritional quality indicators such as soluble solids content, hardness, soluble protein, and total acid in the flesh of seven species of durian. By analyzing volatile substances from seven species of durian, 58 volatile substances were identified, including esters, aldehydes, alcohols, ketones, sulfides, pinenes, acids, thiazoles, pyrazines, and furans. Among them, esters were the highest in D159 durian, aldehydes were the highest in D197, alcohols were the highest in D198 durian, and ketones were the highest in D24. LDA analysis could better distinguish the differences between the flesh flavors of seven species of durian. Using 3D-PCA and matching matrix analysis, the flavor differences of seven species of durian could also be well distinguished. This study provides support for the introduction of new durian varieties, commercial planting, and localized hybrid breeding in China.
  • LI Xianyu, LUO Mingbiao, WANG Weimin, CHENG Xuemeng, ZENG Kai, DONG Yunya, YANG Mei
    Food and Fermentation Industries. 2023, 49(14): 265-271. https://doi.org/10.13995/j.cnki.11-1802/ts.032871
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    An ultrasound-assisted dilute acid extraction method for the simple, rapid and accurate determination of trace cadmium (Cd) in rice was established based on the self-developed miniature hydride generation atomic fluorescence spectrometer. The best auxiliary extraction way was obtained by comparing the stability of four auxiliary extraction ways, including ultrasonication, centrifugation, shaking, and resting. The effects of ultrasonic time, ultrasonic power, hydrochloric acid concentration, thiourea concentration, and cadmium sensitizer concentration on the extraction of cadmium were investigated by single-factor experiments to obtain the optimal extraction conditions. Results showed that ultrasound-assisted extraction had the best way and stability. The optimal extraction conditions were as follows: sonication time of 10 min, sonication power of 80%, hydrochloric acid concentration of 4% (volume ratio), thiourea concentration of 5.0 g/L, and cadmium sensitizer concentration of 2.5 g/L. After optimization, the linearity of Cd was good in the range of 0.05-0.25 ng/mL, and the correlation coefficient was 0.999 8. The detection limit of the method was 0.000 61 mg/kg, the recovery rate was 93.3%-106.7%, and the relative standard deviation was 1.62%-4.06%. The method was verified by rice standard substances GSB-1 and GSB-22, and the results were accurate and reliable. Compared with the traditional method, the method has simple pretreatment, short time consumption, and accurate determination, and is suitable for the determination of trace cadmium in rice.
  • LIANG Jing, ZHAO Xiangmin, LI Lulu, KANG Jing, TANG Defu, LIU Jia, GUO Yiwen, YANG Tao, HAO Shengyan, DOU Xiaoli, SONG Shuzhen
    Food and Fermentation Industries. 2023, 49(14): 272-279. https://doi.org/10.13995/j.cnki.11-1802/ts.034102
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    This study applied near-infrared spectroscopy combined with chemometric methods to rapidly predict the nutrient content of Minqin lamb (a geographical indication product), aiming to provide a theoretical basis for the quantitative detection of the nutrient content of Minqin lamb and technical support for the nutritional properties and product value of Minqin lamb. A total of 269 meat samples were collected from the longest back muscle, foreleg meat, and hind leg meat of 90 sheep in Minqin, and near-infrared spectra were collected. The calibration models of lamb muscle fat, protein, and fatty acid content were established by using various pretreatment methods, including principal component regression (PCR), partial least squares (PLS), and modified partial least squares (MPLS). Except that the best modeling method of stearic acid was partial least squares, the best modeling method of other indicator models was improved partial least squares. Among them, the RSQ of fat, protein, and monounsaturated fatty acid models were higher than 0.9, which were 0.936, 0.916, and 0.911, respectively. The RSQ values of palmitic, oleic, linoleic, saturated, and polyunsaturated fatty acid models were greater than 0.8 and less than 0.9, which were 0.806, 0.843, 0.883, 0.852, and 0.895, respectively, while the RSQ values of myristic acid and stearic acid models were less than 0.7, which were 0.323 and 0.561, respectively. The application of the near-infrared technique combined with chemometric methods can effectively predict the nutritional composition of lamb muscle.
  • JIANG Chunyang, DENG Qiaosheng, WANG Dan, XU Lingfeng, YANG Qingshan, ZHANG Hua, YANG Qin, SHI Rujie, ZHOU Nong
    Food and Fermentation Industries. 2023, 49(14): 280-287. https://doi.org/10.13995/j.cnki.11-1802/ts.033311
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    To optimize the determination method for the total flavonoids in Lycopus lucidus, this study compared the absorption spectra of 6 reference substances and the samples in the three color reaction systems of NaNO2-Al(NO3)3-NaOH, AlCl3-Kac, and triethylamine. The response surface-Box-Behnken design optimization was carried out to optimize the influence of the color reagent dosage on the measurement results of total flavonoids in color reaction system of NaNO2-Al(NO3)3-NaOH. The uncertainty evaluation of the preparation of the test solution, the preparation of the reference solution, the color reaction system, and the standard curve fitting was carried out. The combined uncertainty also was calculated, and the expanded uncertainty and the confidence level of the measurement result were given. Through adaptability analysis, NaNO2-Al(NO3)3-NaOH color reaction system was finally selected in the experiment, and the optimal color reaction conditions were as follows: 0.9 mL of 50 g/L sodium nitrite, 0.6 mL of 100 g/L aluminum nitrate, 4.8 mL of 1 moL/L hydrogen sodium oxide, and color reaction temperature was 42 ℃. The content of total flavonoids was measured at (91.052±2.75) mg under optimal conditions, which provided a new reference method for the determination of total flavonoids.
  • HOU Yingying, ZHANG Hongjie, MA Junyi, ZHU Jianzhao, MIAO Xin, HE Qingyan
    Food and Fermentation Industries. 2023, 49(14): 288-294. https://doi.org/10.13995/j.cnki.11-1802/ts.033281
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Sixteen varieties of Zanthoxylum bungeanum from the germplasm bank of the Economic Forest Research Institute of Longnan City, Gansu Province were used as the test materials to construct gas chromatography-mass spectrometry (GC-MS) fingerprints. The volatile components of 16 varieties of Zanthoxylum bungeanum were analyzed and identified by GC-MS, and 16 varieties of Zanthoxylum bungeanum were classified and analyzed by clustering analysis and principal component analysis. The fingerprint was constructed by the Computer Similarity Evaluation System of Traditional Chinese Medicine Chromatographic Fingerprint (2012A version). Results showed that the volatile components of 16 varieties of pepper were similar to little difference, which could be divided into alcohols, olefins, esters, ketones, aldehydes, and benzenes, among which the kinds of alcohols and olefin were more and the relative content was higher. The classification results of cluster analysis and principal component analysis showed that there were no significant differences among 16 varieties of Zanthoxylum bungeanum. The GC-MS fingerprints of volatile components of Zanthoxylum bungeanum were established and 11 common peaks were identified as characteristic peaks of Zanthoxylum bungeanum. Combined with Angle cosine and Pearson correlation coefficient analysis, the similarity of them was all above 0.9, which indicated that the fingerprint establishment method was effective. The research could provide theoretical support for the identification of production area, quality detection, variety classification, and evolutionary relationship of Wudu Zanthoxylum bungeanum.
  • ZHANG Aolin, LI Shen, ZOU Yingfang, WANG Jifen, ZHANG Zhen, SUN Yijian
    Food and Fermentation Industries. 2023, 49(14): 295-301. https://doi.org/10.13995/j.cnki.11-1802/ts.033379
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    The spectral information of 70 groups of coffee containing sibutramine in 0-2.5 THz band was collected by terahertz time-domain spectroscopy. Three pattern recognition methods, including random forest, support vector machine, and Bayesian discriminant analysis, were established and compared. The model recognition accuracy without preprocessing was lower. The first derivative, second derivative, different types of Butterworth filters, and Pearson feature selection fusion spectroscopy method were selected for spectral signal processing. The accuracy of the Bayesian discriminant analysis model based on first derivative processing was 98.6%, the classification accuracy of the random forest model based on the high-pass Butterworth filter was 94.2%, and the classification accuracy of the fusion spectral support vector machine (SVM) model based on feature extraction was 100%. The SVM model with optimal pretreatment was selected to further identify adulterated coffee in different areas of the same brand, and the accuracy rate was 100%. The two-level feature recognition of “brand-origin” was realized, which could provide certain help for the public security organs to crack down on food safety crimes involving coffee.
  • WANG Jiaying, LIU Jia, LEI Lin, ZHAO Guohua, YE Fayin
    Food and Fermentation Industries. 2023, 49(14): 302-310. https://doi.org/10.13995/j.cnki.11-1802/ts.032756
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Cereal grains are raw materials for making processed staples of human diets, in which the main constituent is starch. Due to the characteristics of convenience and green, the dry heating treatment (DHT) process has been used increasingly for starch modification in the food industry. It has been proved that DHT effectively improves both the properties of cereal starches and the quality of cereal foods. This review focused on the research advances in cereal starch modifications by DHT. It not only described the principle of DHT as a method of altering starch properties but also outlined the effect of dry heating on the composition, structure, and physicochemical properties of cereal starches, and the benefits of added ingredients (such as ionic gums, amino acids, sugars, and so on) on assisting dry heating treatments of cereal starches. The applications of DHT as an alternative for cereal starch modification in the food industry were also summarized. In addition, the challenges in the dry heating modification improving the functionalities of cereal starches prospected. At the end of the paper, the direction of the future research was discussed.
  • ZHU Jiamin, WU Yi, ZHAO Linjing, SHEN Fanni, CAO Tingwei, LIU Xiaohui, LIU Xijian, LI Hongsen, LI Qi, FENG Meiqing
    Food and Fermentation Industries. 2023, 49(14): 311-320. https://doi.org/10.13995/j.cnki.11-1802/ts.035592
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Hericium erinaceus polysaccharide is a kind of biological macromolecule extracted from the fruiting body, mycelium or fermentation broth of the culinary and medicinal mushroom of H. erinaceus. In recent years, more and more studies have shown the roles of H. erinaceus polysaccharide in immune regulation, alleviating inflammatory bowel disease, and improving nervous system and metabolic diseases by mediating gut microbiota. H. erinaceus polysaccharide possess a variety of health promoting effects by enriching the intestinal probiotics, inhibiting the growth of pathogenic bacteria, improving the production of short-chain fatty acids, enhancing intestinal immune responses, and activating the specific signaling pathways. These biological activities are related to the structure of H. erinaceus polysaccharide. This review summarized the structural characteristics of H. erinaceus polysaccharide from different sources and the influence on gut microbiota diversity, the effects and potential mechanism on promoting health by regulating intestinal flora, as well as the applications and development prospects in various fields. It could provide a theoretical basis for further study of H. erinaceus polysaccharides on gut microbial homeostasis, and give a reference for developing and utilizing H. erinaceus polysaccharides as promising prebiotics.
  • LI Jianjun, QU Chunyun, LIU Gongliang, BAI Weidong, XIAO Gengsheng, GAO Hong, FANG Fang, ZHENG Yu
    Food and Fermentation Industries. 2023, 49(14): 321-329. https://doi.org/10.13995/j.cnki.11-1802/ts.035315
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Higher alcohols are a type of flavoring substance widely existed in alcoholic beverage, which could enrich the taste of beverages at moderate amount. However, it may cause headaches, discomforts, and serious harms to humans after consumption at high levels. This paper highlights both the mechanism of harmful effects of higher alcohols on humans, and the non-genetic engineering methods to reduce the content of higher alcohols in beverage wines, especially stating the effect of the yeast assimilable nitrogen sources addition on higher alcohols synthesis, as well as its regulation mechanism. At the same time, methods to reduce the content of higher alcohols are also proposed, including both fermentation process optimization and post-processing methods to establish a feasible and easy-to-use scheme to reduce higher alcohols for industrial brewing, which could provide a reference and the technical support for industrial brewing and scientific drinking.
  • LIANG Xinfu, DONG Qingliang, GAO Jiatao
    Food and Fermentation Industries. 2023, 49(14): 330-337. https://doi.org/10.13995/j.cnki.11-1802/ts.033424
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Type 2 diabetes mellitus(T2DM) is an epidemic chronic metabolic disease with an increasing number of patients year by year. Due to factors such as impaired metabolic function, it can generally cause a variety of complications, which seriously threatens human health. As the oligosaccharide with the highest content in traditional Chinese medicine Rehmannia glutinosa, modern research has shown that it also has the function of lowering blood sugar. Moreover, stachyose can also have a variety of positive effects on the human body by proliferating intestinal probiotics, which can improve the complications of T2DM, and has the potential to become a new natural hypoglycemic drug. This article expounds the research progress and potential mechanism of stachyose and T2DM, in order to provide a theoretical reference for the development of stachyose products for the treatment of T2DM and related treatments.
  • LIU Chunjie, ZHANG Tao, ZENG Zhiguo, SHANG Yongbiao
    Food and Fermentation Industries. 2023, 49(14): 338-345. https://doi.org/10.13995/j.cnki.11-1802/ts.032821
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Livestock and poultry meat is the main component of animal food consumption in China, including pork, beef, mutton, chicken, duck, and goose, rabbit meat, etc. Livestock and poultry meat usually has a certain fishy taste, if it is not processed properly, it will affect the quality of the product and thus affect the acceptance of the product by consumers. Previous research on fishy substances has mainly focused on the field of aquatic processing, and in recent years, the research on fishy substances in livestock and poultry meat has also attracted more and more attention. In this paper, the main components and contents of fishy substances in different livestock and poultry meats, the detection methods and removal methods of fishy substances were reviewed to provide a reference for subsequent research on livestock and poultry meat, and also provide scientific guidance on how to reduce the fishy taste during the processing of livestock and poultry meat.
  • XIAO Yuanyuan, XU Chenfeng, WANG Lulu, ZHANG Chi, DENG Lingli, SHANG Longchen
    Food and Fermentation Industries. 2023, 49(14): 346-356. https://doi.org/10.13995/j.cnki.11-1802/ts.035226
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Dietary fiber (DF) is a carbohydrate polymer mainly found in plant cell walls, which possesses physiological effects such as enhancing satiety, reducing cholesterol absorption, delaying blood glucose elevation, and regulating intestinal flora. However, the physiochemical properties of the natural DF, including solubility, rheological properties, and interfacial properties, are often difficult to meet the growing demands of the food industry. Therefore, how to accurately and efficiently modify the main physicochemical of the DF has become a research hotspot in the DF processing field. Increased researchers are favored in physical modification for its efficiency, greenness, and easy operation. These physical treatments are often applied to improve the DF′s functional properties to expand their applications in the food industry. The present paper briefly described the structure and classification of DF and reviewed the physical basis of the nutritional effects of DF and the effects of different physical modification techniques on the DF′s physical and functional properties, which was expected to provide some theoretical support and ideas for the nutritional study of the DF′s physical properties.
  • LIAO Yuesheng, YAN Caiqing, LUO Changwei, LIU Hongwu
    Food and Fermentation Industries. 2023, 49(14): 357-361. https://doi.org/10.13995/j.cnki.11-1802/ts.032661
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Sports nutrition supplement is an important means for athletes to improve sports performance, prevent sports injury, and speed up the recovery of sports fatigue. Reasonable planning of sports nutrition supplements is the key to improving sports performance. In recent years, a plant-based diet has received extensive attention in the field of sports nutrition. It is found that a plant-based diet could provide a wide range of health benefits for athletes, including promoting athletes′ health and improving sports performance. However, there is no systematic study on the relationship between plant-based diet and exercise. Therefore, this paper combed and summarized relevant research and application results through databases such as CNKI, Wanfang, VIP, Web of Science, PubMed, etc., providing some references for the application of plant-based diet in the field of sports nutrition.
  • ZHANG Congchao, YANG Bo, YANG Guang, CEN Tao
    Food and Fermentation Industries. 2023, 49(14): 362-368. https://doi.org/10.13995/j.cnki.11-1802/ts.032688
    Abstract ( ) Download PDF ( ) HTML   Knowledge map   Save
    Accelerated aging of paddy can make fresh paddy complete the quality requirements of paddy that need to be stored naturally for more than one year in a short period. The aging of paddy is mainly influenced by temperature, moisture, and time, and accelerated aging mainly starts from these three factors. At this stage, the main methods of accelerated aging are wet heat treatment, microwave treatment, radiation treatment, high-temperature fluidized bed combined with tempering treatment, chemical pretreatment, etc. Accelerated aging has a certain degree of influence on the pasting properties, thermodynamic properties, digestibility, and cooking properties of rice grains. Compared with different accelerated aging methods from microstructure, starch, protein, lipid, and other component interactions on functional property changes and aging mechanism previously explored and analyzed, the aging mechanism of rice is still not fully understood. Now, a systematic review of the research progress of accelerated aging of rice was made, hoping to provide a theoretical basis for shortening the aging time of rice and better application of rice and to provide some theoretical help for researchers.