25 May 2021, Volume 47 Issue 10
    

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  • LI Jiayi, FANG Jiaxing, LI Yalong, SUN Wenjia, LIU Ping, WANG Qin
    Food and Fermentation Industries. 2021, 47(10): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.025302
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    In order to explore the effect of protein modification on the properties of complex coacervation, hydrolyzed soy protein isolate (HSPI) and sodium alginate (SA) with a degree of hydrolysis at 2% was used as complex wall materials to prepare multipolymer and complex orange oil microcapsules. Taking the ζ-potential and turbidity titration curves as the main indicators, the optimal conditions for complex coacervation were m(HSPI)∶m(SA)=7∶1, pH 3.5, and 25 ℃. Taking SPI-SA complex coacervation microcapsules (ISC) as a control, the characteristic of HSPI-SA microcapsules (HSC) was determined, where the moisture content, emulsification stability and angle of repose HSC were all less than ISC, the effective load and HSC in emulsification activity index were higher than those of ISC. Thermogravimetric analysis showed that the heat resistance of HSC was better than that of ISC. Using solid phase micro-extraction-gas chromatography-mass spectrometry to analyze the HSC volatile substances under different heating times at 80 ℃, it was found that the content of seven sweet orange oil characteristic flavor substances decreased with the extension of the heating time. According to the lowest content, HSC can withstand heating in a water bath within five hours at 80 ℃.
  • JI Mengmeng, JIANG Jing, FAN Liuping, LI Jing
    Food and Fermentation Industries. 2021, 47(10): 8-13. https://doi.org/10.13995/j.cnki.11-1802/ts.025551
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    Compared with the traditional food thermal processing, far infrared irradiation (FIR) was more efficient and energy savings without chemical residues, which had a broad application prospect in food sterilization. The objectives of this study were to evaluate the effect of FIR on the inactivation of Aspergillus flavus spores inoculated in rice and the reduction of aflatoxin B1 production. In addition, changes of the cell morphological of A. flavus spores were studied after FIR treatment. When the moisture content of rice was 20% and 30%, FIR heating at 115 ℃ for 5 min resulted in (2.04±0.17) and (2.96±0.28)-log reductions of A. flavus spores, respectively. The total amount of AFB1 decreased by 56.61% and 63.34%, respectively. And the amount of AFB1 produced by unit dry weight of the mycelium decreased by 27.64% and 38.94%, respectively. After FIR treatment, MTT measurement showed a decrease in the viability of spores. A significant increase in number of cells stained by propidium iodide was observed using fluorescence microscopes. Cell membrane damage and the leakage of intracellular substances of A. flavus spores were observed by SEM. Results of circular dichroism spectroscopy (CD) suggested protein denaturation accelerated and the relative content of random curl increased when FIR combined with tempering treatment, which induced the inactivation of A. flavus spores. The FIR treatment affected the growth and metabolism process of A. flavus spores with the destruction of cell structure. As a result, the risk of A. flavus spores and AFB1 infection in grain was reduced after FIR treatment.
  • ZHENG Kai, ZHANG Min, FANG Jiaqi, LING Yu, LI Qixun, JIA Miao
    Food and Fermentation Industries. 2021, 47(10): 14-21. https://doi.org/10.13995/j.cnki.11-1802/ts.026114
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    The effects of step heating treatment on storage quality and reactive oxygen species metabolism of postharvest zucchini fruits during cold storage [ (4±0.5)℃, (80±5)% RH] for 15 days were investigated in this paper. 129 fresh “Lvfeng” zucchinis were divided into three groups. One group of zucchinis were immersed in hot water at 32 ℃ for 14 min and then with the treatment at 44 ℃ for 14 min (step heating treatment). The other two groups of zucchinis were immersed separately in 44 °C hot water (constant temperature treatment) and then in 20 °C (CK) clear water of for 28 min. The results showed that compared with constant temperature treatment, step heating treatment powerfully stimulated the high activity of superoxide dismutase (SOD) in the first 5 days and peroxidase (POD) in the last 5 days which resulted in the increase of glutathione (GSH), the reduction of malondialdehyde (MDA) (P<0.05) and the activity of superoxide anion (O-2·). In addition, it also delayed chilling injury and tissue softening, inhibited the increase of weight loss rate, maintained better peel color (P<0.05) and highly soluble solid content. However, there was no significant difference in stimulating high activity of catalase (CAT). Furthermore, the results showed that the heating rate of tissue was more sensitive than the temperature of tissue in characterizing temperature field change. Based on the analysis of the temperature field of zucchini, the heating rate of tissue may be an important factor affecting storage quality and reactive oxygen species metabolism of zucchini during low-temperature storage.
  • WANG Ziyu, WANG Zhiying, LUO Gang, LEI Ailing, CHEN Hourong, ZHANG Fusheng
    Food and Fermentation Industries. 2021, 47(10): 22-29. https://doi.org/10.13995/j.cnki.11-1802/ts.026543
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    Recently, a large number of consumers have paid attention to the taste of orange juice. To improve the taste of orange juice and provide the theoretical basis for processing, high pressure homogenization (HPH) at 0-100 MPa was applied to treat orange juice. Then the changes of its rheological properties were measured, the pectin and other dietary fibers which had a major influence on the rheological properties were also been determined. The results showed that, the flow coefficient n value of orange juice increase by 19.94%-43.2% after treatment. The viscosity coefficient K value and apparent viscosity (at 0.1 rad/s shear rate) showed a downward trend which decreased by 22.86%-77.1%, 2.52%-74.7% respectively. The critical gelation point was in advance with the storage modulus G′ and the loss modulus G″ presented a decreasing trend. Moreover, time and temperature scanning had similar results with apparent viscosity. The friction coefficient μ value at the slip rate of 0.2 m/s decreased by 85.1%-92.2%. And the HPH treatment caused the lower particle size, the more narrow distribution, and the higher Zeta potential value. The molecular weight (Mw) of soluble dietary fiber and cellulose in orange juice decreased by 0.024%-1.87% and 9.14%-59.97% respectively. Furthermore, the results of Fourier infrared (FTIR) and scanning electron microscopy (SEM) measurement also proved that the destruction of the polysaccharide solute particle structure by HPH caused the changes in the rheological properties of orange juice. It is proved that HPH treatment can change the rheological properties of orange juice by reducing the viscosity and viscoelasticity to improve drinking comfort.
  • YOU Yuming, WANG Yugui, ZHANG Jie, ZHENG Jiong, WU Liangru
    Food and Fermentation Industries. 2021, 47(10): 30-36. https://doi.org/10.13995/j.cnki.11-1802/ts.025880
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    In this study, the effects of high-pressure homogenization (HPH) treatments on the physicochemical and structural properties of dietary fiber from bamboo shoots were explored. Results showed that the particle size of bamboo shoots dietary fiber (BSDF) decreased significantly (P<0.05) by the treatment of HPH, however, the water holding capacity (WHC), oil holding capacity (OHC), swelling capacity (SC), and glucose and nitrite ion absorption capacity were increased remarkedly (P<0.05). When the pressure of HPH was 100 MPa, the particle size (630.56±28.09) nm of BSDF was smallest, WHC, OHC and SC were increased by 42.26%, 53.30% and 52.47% respectively, and the adsorption capacity of glucose and nitrite also reached maximum. Results of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and thermogravimetric analysis showed that the crystallinity of BSDF was decreased after HPH, the cellulose, hemicellulose and lignin partially degraded, the thermal stability was decreased, and the surface structure was loose. However, the agglomeration occurred. In conclusion, HPH treatment can effectively improve the physicochemical and structural properties of BSDF, which can be used as an excellent method to modify dietary fiber.
  • LIANG Qingwen, ZHOU Chaohui, LI Tieqiao, LU Liling, LIU Jiale, FANG Fang
    Food and Fermentation Industries. 2021, 47(10): 37-42. https://doi.org/10.13995/j.cnki.11-1802/ts.025431
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    The presence of spoilage bacteria in raw materials may have adverse effect on food quality during the processing and/or storing. To identify the microbes that may cause the liquefaction and change the quality of oyster sauce by starch degradation, the bacterial compositions in oyster sauce and its main raw materials (oyster juice and flavorant) were analyzed using the Illumina Miseq sequencing platform. The results showed that the dominant bacteria in oyster sauce were Planomicrobium (55.17%), Xanthomonas (16.49%) and Bacillus (13.78%). Planomicrobium (45.83%), Tetragenococcus (15.35%), Bacillus (13.63%) and Lactobacillus (11.66%) were the dominant genera in oyster juice, while Planomicrobium (55.03%), Tetragenococcus (20.36%) and Bacillus (6.88%) were the major bacteria in flavorant. The dominant bacteria in semi-finished oyster sauce prepared by mixing gelatinized starch with oyster juice and flavorant were Planomicrobium and Bacillus, respectively. Five starch utilizing strains were isolated from starch-hydrolyzed semi-finished oyster sauce and were identified as Staphylococcus hominis S1, Bacillus velezensis S2 and S4, Bacillus subtilis S3 and S5. Moreover, these strains were also found in oyster juice and flavorant, but their content increased significantly in starch-hydrolyzed semi-finished oyster sauce. These results provide references for the control of spoilage bacteria in manufacturing starch containing condiments, which is of great significance to establish the traceability technology of microorganism related to food spoilage.
  • YE Xin, WANG Li, JIANG Chao, DU Xiaojing, DING Xiaoyu, WANG Hongxin
    Food and Fermentation Industries. 2021, 47(10): 43-49. https://doi.org/10.13995/j.cnki.11-1802/ts.025625
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    In order to efficiently extract caffeine from waste tea resources and promote the development and utilization of tea caffeine, the raffinate after extracting tea polyphenols by liquid-liquid extraction was used as raw material to purify caffeine through sublimation. Effects of pretreatment agents and sublimation conditions on caffeine yield were investigated, and response surface methodology was used to optimize the process parameters, followed by analyzing the inhibition of caffeine on collagenase and elastase in vitro. The optimum sublimation conditions were as follows: sodium carbonate was used as pretreatment agent, the mass ratio of sodium carbonate addition to solidified raffinate was 2.3∶1, the sublimation temperature was 214 ℃, and the sublimation time was 28 min. Under these conditions, the yield of caffeine was 76.21%, and the purity of caffeine exceeded 98.5%. Furthermore, tea caffeine had an excellent inhibitory activity on collagenase and elastase, and the inhibition rates at 1 mg/mL were 46.72% and 38.43%, respectively. Compared with the traditional process, this process not only greatly improves the yield of caffeine, but also is much more economical and safer, and has a great industrial application prospect. Meanwhile, the obtained tea caffeine can be used as a natural collagenase and elastase inhibitor, and get a further utilization in related skin care products.
  • LI Xiaonan, LI Yuhui, LU Shiling, WANG Jungang, LI Baokun, LIU Shilin
    Food and Fermentation Industries. 2021, 47(10): 50-57. https://doi.org/10.13995/j.cnki.11-1802/ts.025978
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    In this experiment, we optimized lipase-producing lactic acid bacteria in 23 handmade cheese samples from Yili, Hami and Tacheng regions in Xinjiang. Traditional cultivation of lactic acid bacteria combined with tributyrin plate and copper soap method were used for screening. Four strains of lipase-producing lactic acid bacteria were screened out. Among them, B2-5 was identified as Lactobacillus plantarum, T1-5 and T1-3 was Weissella confuse, H1-6 was Lactobacillus helveticus. And the enzyme production characteristics and enzymatic properties of these strains were studied. The results showed that: when the inocu lum amount was 2%, the initial pH was 7 and the fermentation medium was added with polyvinyl alcohol (PVA), and fermented at 37 ℃ for 48 h L. plantarum B2-5 had the highest enzyme production, the enzyme activity reached 5.29 U/mL. L. helveticus H1-6 had an enzyme activity of 3.46 U/mL, W. confuse T1-5 and T1-3 produced large amounts of enzymes and had good tolerance to fermentation conditions. The addition of K+, Na+, Fe2+, Mn2+ to the fermentation broth significantly promoted the enzyme production of the four lactic acid bacteria (P<0.05). The lipases were all acidic and medium-temperature lipases, which performed better under acidic conditions.
  • ZENG Lingjie, HUANG Jinxiang, FENG Pixue, AN Jiaxing, SI Zaiyong, LONG Xiufeng, YI Yi
    Food and Fermentation Industries. 2021, 47(10): 58-65. https://doi.org/10.13995/j.cnki.11-1802/ts.025766
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    Formic acid, a small molecule acid, is a representative cell inhibitor in lignocellulose hydrolysate, which can inhibit cell growth during the alcohol fermentation of lignocellulose. The differentially expressed genes (DEGs) of Saccharomyces cerevisiae before and after 1.8 g/L formic acid treatment were analyzed by RNA-seq analysis. The results indicated that under formic acid stress, a total of 1 504 DEGs were identified, of which 797 were up-regulated genes and 707 were down-regulated genes. The enrichment analysis of differential genes through GO and KEGG found that these DEGs mainly involved in the pathways including ribosome pathway, meiosis-yeast, MAPK signaling pathway, glycolysis/gluconeogenesis pathway, starch and sucrose metabolism pathway, cysteine and methionine metabolism pathway, and purine metabolism pathway. This study preliminarily revealed the molecular response mechanism of S. cerevisiae under formic acid stress, and can provide a theoretical basis for further research on methods to improve the tolerance of cell inhibitors in lignocellulose hydrolysate.
  • CHEN Jinfeng, ZHANG Shenggui, MA Yunxiang, WANG Yue, XU Xiaoqin, CUI Yanli
    Food and Fermentation Industries. 2021, 47(10): 66-71. https://doi.org/10.13995/j.cnki.11-1802/ts.025557
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    In order to study the effect of potato starch on dough gelatinization, rheology and texture, 0%, 5%, 10%, 15% and 20% potato starch were added to low gluten wheat flour by substitution method. The thermodynamic properties, dynamic rheological properties, texture properties, microstructure and secondary structure of gluten were measured. The results showed that by adding potato starch, the gelatinization starting temperature and peak temperature decreased significantly, meanwhile, the gelatinization enthalpy increased significantly. The dough began to gelatinize when the temperature was higher than 50°C. The more potato starch was added, the more dough tended to be solid material. The hardness, chewability and elasticity of dough decreased significantly with the addition of potato starch. The microstructure of dough showed that when the amount of potato starch was less than 15%, the damage of gluten network was relatively small and the structure was relatively stable. Considered comprehensively, when the amount of potato starch is 10%, the dough has good gelatinization, rheology, texture and gluten network structure.
  • ZHOU Yanqing, LIU Ting, ZHOU Wanting, GUO Lidan, ZHAO Shuaidong, WANG Liping
    Food and Fermentation Industries. 2021, 47(10): 72-78. https://doi.org/10.13995/j.cnki.11-1802/ts.025765
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    Acetic acid bacteria (AAB) is an important microorganism for the production of acetic acid and its flavor derivatives during fruit vinegar brewing. High acetic acid-yield AAB with ethanol or temperature tolerance is of great significance to improve the quality of fermented fruit vinegar in China. In this study, nine high-yield AAB were isolated by enrichment, calcium circle, qualitative and quantitative analysis from nine kinds of fruit rot parts in different regions of China. Under different stress environments (high ethanol concentration or high temperature), the growth ability of high-yield acid strains was compared using an automatic growth curve instrument. The strain with excellent tolerance was applied to plum vinegar brewing and identified by morphology and 16S rDNA molecular. The results showed that Acetobacter sp. LO2 (nos. MN602472) not only produced more acid than Acetobacter pasteurianus AS1.41, but also grew well at 37 ℃ or 11% (V/V) ethanol (culture medium), with its OD600 up to 0.56 and 0.61, respectively. Under suitable, high ethanol [8% (V/V) ethanol] and high temperature (37 ℃) conditions, the plum vinegar produced by LO2 strain not only had high acid production (53.88, 59.80 and 46.64 g/L), but also had good taste. Therefore, Acetobacter sp. LO2 is a special high-yield AAB with excellent tolerance for fruit vinegar, and has a certain application prospect in the field of fruit vinegar brewing.
  • LIU Jiaqi, DU Haibo, CHEN Yiwen, ZHANG Caiwen, XIN Di, SHI Liang, LI Jinxia, CUI Shenghui, YAO Su
    Food and Fermentation Industries. 2021, 47(10): 79-86. https://doi.org/10.13995/j.cnki.11-1802/ts.025740
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    The growth characteristics of test strains Escherichia coli CICC 24176 and CICC 24652 on different medium were studied to evaluate the feasibility of quality control for medium in food microbiological examination. According to the national standards GB 4789.28—2013, the growth rate, selectivity and specificity of these two test strains were determined on specified media, and ATCC 25922 was used as the control strain. The media was consisted of 58 kinds of medium which can be divided into six types: non-selective separation and counting solid medium, selective separation and counting solid medium, selective enrichment medium, selective liquid counting medium, suspension medium and identification medium. Results showed that the growth rates of strain CICC 24176 and CICC 24652 on separation and counting solid medium were all greater than 0.7. Strains grew well on selective liquid counting medium, and growth was effectively inhibited on selective solid and suspension medium. And typical biochemical characteristics were observed on specific medium. The strain CICC 24176 was effectively inhibited on 8 kinds of selective enrichment media except SC liquid medium, while CICC 24652 was effectively inhibited on all selective enrichment media. Strain CICC 24176 had typical biochemical characteristics on 18 kinds of identification media, while strain CICC 24652 had typical biochemical characteristics on 16 kinds of identification media except for SIM medium and motility nitric acid test medium. In conclusion, the test strains can be used as quality evaluation of the microbial performance indicators on corresponding medium.
  • YE Zi, SHANG Zhixun, LI Meiqi, REN Hongbing, QU Yonghan, HU Xiaosong, YI Junjie
    Food and Fermentation Industries. 2021, 47(10): 87-95. https://doi.org/10.13995/j.cnki.11-1802/ts.025920
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    Fermented Xiaomila (Capsicum frutescens L.) is one of the traditional food, which is favored by consumers in Southwest China. In order to study the quality characteristics of Xiaomila, nine cultivars were selected. The color, texture, pungency, VC, and free amino acid of Xiaomila before and after fermentation were quantitatively analyzed, and multivariate data analysis was applied to investigate the effect of cultivars on the quality of fermented Xiaomila. The results showed that the quality indicators were L* value (61.06-69.32), a* value (-6.62--4.02), b* value (44.26-52.20), hardness (1.87-12.92 N), capsaicin (0.24-6.19 mg/g), dihydrocapsaicin (0.13-3.15 mg/g), VC (49.20-69.83 mg/100g), total free amino acids (274.44-467.79 mg/100g). After fermentation, the L* value decreased, and the a* and b* values increased. In addition, chlorophyll, hardness, chewiness, capsaicin, and VC content decreased, but the content of carotenoid, total free amino acids and taste-active amino acids increased. The results of multivariate data analysis showed that fermentation significantly changed the quality characteristics of Xiaomila and reduced the differences among different cultivars to a certain extent. Comprehensive analysis showed that P5 and P6, with high hardness and low spiciness, were suitable for the development of fermentation products with low spiciness. P1, with moderate spiciness and the highest content of taste-active free amino acids, was more suitable for the processing of traditional fermented pepper.
  • CAO Yu, DENG Zeyuan, WEI Changhao, YU Chengwei, LI Hongyan
    Food and Fermentation Industries. 2021, 47(10): 96-102. https://doi.org/10.13995/j.cnki.11-1802/ts.025704
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    Seven dominant strains were screened from the naturally fermented Cuocai (traditional Chinese fermented vegetables) to ferment vegetables. Strains with good fermentation performances and without antagonism effects were selected. The optimal proportion of selected strains was obtained according to the texture analysis, color determination and sensory evaluation. Finally, the volatile flavor compounds of the natural fermented and selected compound starter fermented Cuocai were compared. Results showed that these seven strains had good fermentation performance, and the comprehensive evaluation of fermented Cuocai was the best under the conditions of Pichia kudriavzevii Kazachstania bulderi =1∶2, Lactobacillus buchneriLactobacillus plantarumLactobacillus brevisLactobacillus sunkii=1∶1∶1∶2. The varieties and relative contents of flavor substances including alcohols, ketones, esters and acids in the compound starter fermented Cuocai were higher than those in natural fermented Cuocai.
  • WU Zhen, WU Yuzhang, CHEN Li, WANG Sha, LI Rongyuan, LU Hongmei
    Food and Fermentation Industries. 2021, 47(10): 103-108. https://doi.org/10.13995/j.cnki.11-1802/ts.025936
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    The mulberry pomace vinegar was fermented by solid state fermentation with mulberry pomace as main material and mulberry leaf as auxiliary material. The changes of organic acids and volatile flavor substances during the fermentation of mulberry pomace vinegar were investigated and compared with the mulberry vinegar fermented by conventional liquid fermentation. The results showed that the content of non-volatile acid, total polyphenol and flavonoid in mulberry pomace vinegar was higher than that in mulberry juice vinegar. During the process of mulberry pomace vinegar fermentation, the content of oxalic acid, tartaric acid and malic acid gradually decreased, while the content of succinic acid increased. The content of other organic acids in mulberry pomace vinegar was higher than that of mulberry juice vinegar except for acetic acid and tartaric acid. GC-MS analysis showed that acids, esters and alcohols changed obviously during fermentation, and the composition of the main flavor substances in samples were quite different in each stage. A total of 132 flavor substances were detected in mulberry pomace vinegar, which were more abundant than mulberry juice vinegar (86 flavor substances), and the flavor characteristics were more obvious. This study provides new ideas and technical support for the resource utilization of mulberry by-products.
  • CAO Shiyu, ZHANG Xiaoning, ZHAI Xijiao, ZAHNG Weixing, LIU Tingting, WEI Xuyang, ZHENG Shaowen, LEI Fengjin
    Food and Fermentation Industries. 2021, 47(10): 109-115. https://doi.org/10.13995/j.cnki.11-1802/ts.026410
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    By studying the effects of different drought stress on physiological and biochemical indexes and secondary metabolites of Sonchus oleraceus L., the relationship between functional components metabolism and drought stress was clarified. The results showed that drought stress increased the ratio of root-shoot, and the root-shoot ratio under MST and SST was significantly higher than that of the CK after 10- and 15-days treatment. The soluble protein of SST leaves was significantly higher than that of CK after 10 days drought stress treatment. The activities of SOD, POD and CAT increased under drought stress, however, it decreased with the increase of stress time. After 10 days, the activities of three enzymes under SST were significantly higher than those of CK. When MST and SST were under stress for 10 days, the contents of total phenols and total triterpenes in leaves were significantly higher than CK (P<0.05). In addition, the content of flavonoids in MST and SST were significantly higher than the CK (P<0.05) when the stress was treated for 5 days. Moreover, under mild drought stress for 10 days, the plant could maintain normal physiological and biochemical metabolism, and the content of total phenols and total triterpenoids in leaves increased significantly. The results could be used as the theoretical basis for the irrigation of Sonchus oleraceus L. in Shanxi and improve the functional quality of Sonchus oleraceus L..
  • ZHANG Qiang, WANG Mengjin, MA Yuhan, SUN Yujun, WANG Songhua
    Food and Fermentation Industries. 2021, 47(10): 116-123. https://doi.org/10.13995/j.cnki.11-1802/ts.025831
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    The cytoprotective effects and safety of Morchella protein hydrolysate (MPH) and its selenized derivative (Se-MPH) were studied in this paper.The cytoprotective experiment, performed by employing an H2O2-induced Caco-2 cell model, revealed that both MPH and Se-MPH could protect Caco-2 cells from oxidative injury by alleviating oxidative stress and inhibiting apoptosis.This protective effect may be mainly attributed to the activation of the Nrf2 signaling pathway and the regulation of apoptotic-related protein expressions.The safety evaluation, conducted via cytotoxicity analysis, erythrocyte hemolysis assay, Ames test, and CAM assay, demonstrated no cytotoxicity, hemolytic activity, and mutagenic potential for MPH and Se-MPH.They neither caused visible morphological changes in blood vessels nor led to bleeding in the CAM assay.All these findings suggest that MPH and Se-MPH possess remarkable cytoprotective effects and biological safety, and therefore are expected to be developed and utilized as a potential nutraceutical, functional, or pharmaceutical ingredients.
  • WANG Lijun, LIAO Suqi, LONG Hairong, XIA Xianghua, CHEN Qianping, LIANG Jie, WEI Shugen
    Food and Fermentation Industries. 2021, 47(10): 124-131. https://doi.org/10.13995/j.cnki.11-1802/ts.025882
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    The peel and pulp of a novel fruit, Kadsura coccinea fruit were used as the research objects to compare their nutritional components (including carbohydrate/monosaccharide composition, lipid/fatty acids, crude protein/amino acids, essential oil and minerals). The results showed that the total sugar of the peel and pulp of K. coccinea fruit was 21.14% and 24.15%, and the polysaccharide content was 5.97% and 2.64%, respectively. The analysis of the monosaccharide composition of polysaccharides showed that the peel and pulp polysaccharides all contain 9 kinds of monosaccharides, including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose. However, there were differences in the molar ratio of monosaccharides. The content of fatty acids in the peel and pulp was relatively low, but the unsaturated fatty acids account for a relatively high proportion of 56.21% and 29.39%, and the main components of unsaturated fatty acids were linoleic acid and α-linolenic acid. The K. coccinea fruit contained 17 kinds of amino acids, including 7 kinds of essential amino acids, and the amino acid score results showed that the protein quality of the pulp was better. There were many types of volatile components in the peel and pulp, and terpenes were the main volatile components. In addition, the K. coccinea fruit was also rich in mineral elements, including trace elements such as iron, zinc, manganese and selenium, which were necessary for the human body, and the content of heavy metals was lower than the limit specified by the standard. In summary, the peel and pulp of K. coccinea fruit are rich in nutrients and have good nutritional value. This study provides a reference for further development and utilization of the K. coccinea fruit.
  • HU Li, WANG Fangmei, LYU Mingshan, DING Shuaijie, AIHEMAITIJIANG Aihaiti, BAI Lin, SHI Mengqi, XU Bingjie, WANG Xuguang, XU Xiaopei, RUXIANGULI Maimaitiyiming, WANG Liang
    Food and Fermentation Industries. 2021, 47(10): 132-138. https://doi.org/10.13995/j.cnki.11-1802/ts.025912
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    The present study was conducted to evaluate the quality changes of chicken paste during storage at different temperatures and to establish the shelf life prediction model of the chicken paste by using a first-order kinetic equation and Arrhenius equation. The vacuum-packed chicken paste was pasteurized and stored at different temperatures (4 ℃,25 ℃,37 ℃) for 4 weeks. During the storage period, the total viable counts (TVC), total volatile base nitrogen (TVB-N), acid value (AV) and thiobarbituric acid reactive substances (TBARS) of each sample increased in different degrees, and these amounts increased as storage temperature increased significantly. The water activity of chicken paste in the later stage of storage at 25 ℃ and 37 ℃ was significantly higher than that in the early stage. Compared with the samples stored at 4 ℃, the sensory deterioration of the other two groups was significantly fast and reached an unacceptable level on the 16th day and 12th day, respectively. In addition, the total coliform counts (TCC) index was negative (most probable number, MPN(3) in all samples. Finally, the predictive model of chicken paste with TVC and the relative errors of this model between predicted and actual values of TVC were less than ±10%, indicating that the model is reliable.
  • XU Yongxia, BAI Xuting, QU Shiyao, ZHAO Honglei, LI Xuepeng, BU Ying, LI Jianrong, XIE Jing, JI Guangren
    Food and Fermentation Industries. 2021, 47(10): 139-144. https://doi.org/10.13995/j.cnki.11-1802/ts.025833
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    Pollock was used as raw material to prepare fish soup, and the effect of the different addition amount of crab mushroom on the flavor characteristics of fish soup was studied. Electronic nose, electronic tongue, automatic amino acid analyzer, high-performance liquid chromatography combined with sensory evaluation were used to analyze the flavor difference of fish soup with different amounts of crab mushroom. The results showed that with the increase amount of crab mushroom, the sensory scores except bitter taste and color increased first and then decreased, and the sensory score was the highest when the addition amount was 18%. The smell and taste of fish soup with different amounts of crab mushroom could be effectively distinguished by electronic nose and electronic tongue. With the increase of the number of crab mushrooms, the contents of organic acid and nucleotide in the fish soup increased significantly (P<0.05). The total amount of free amino acids increased firstly and then decreased, and the umami and sweet amino acids were the main flavor amino acids in mushroom fish soup. The addition of crab mushroom reduced the percentage content of umami and sweet amino acids in fish soup, while the percentage content of bitter amino acids increased. Combined with the results of sensory evaluation, the best flavor and taste of the fish soup was obtained when the addition amount of crab mushroom was 18%.
  • YANG Yongan, LI Jingjing, LIU Jianfu, TIAN Jiyuan, ZHU Yiqun
    Food and Fermentation Industries. 2021, 47(10): 145-150. https://doi.org/10.13995/j.cnki.11-1802/ts.025815
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    In this study, ultra-low temperature (-50±0.1) ℃ frozen salmon were used as a control to study the effect of different temperature fluctuations (±2, ±1, ±0.5 ℃) under low temperature (-18 ℃) environmental conditions on the texture of salmon (hardness,elasticity, chewiness, tenderness), color difference, sensory and other physical and chemical indicators in order to explore the impact of different temperature fluctuations on the quality of frozen salmon, and compared the quality of frozen salmon with ultra-low temperature (-50±0.1) ℃. The result showed that the chromatic aberrations of salmon increased with the extension of frozen storage time, and the texture characteristics such as chewiness, hardness and elasticity of salmon decreased significantly with the extension of storage time. The temperature fluctuation range under -18 ℃ frozen storage conditions had a significant difference on the chromatic aberrations and texture (elasticity, hardness, chewiness, tenderness) of salmon (P<0.05), and compared with (-18±2) ℃ and (-18±1) ℃ group, (-18±0.5) ℃ could extend the storage time by 1-2 months. In short-term storage, reduced the temperature fluctuation range to±0.5 ℃, and under the condition of ensuring the same freezing rate, the frozen storage temperature of salmon could be increased from -50 ℃ to -18 ℃. Moreover, the texture (hardness, elasticity, chewiness, tenderness) of salmon affect each other, and the correlation coefficients were all greater than 0.84. Taking frozen storage time and temperature fluctuation range as independent variables, and elasticity, chewiness and tenderness as dependent variables, a multiple linear regression fitting equation for salmon texture characteristics were established to provide a basis for establishing shelf-life prediction.
  • XIONG Zeyu, XIE Chen, CHEN Baike, LI Hui, JIN Sulaiman, BAO Hairong
    Food and Fermentation Industries. 2021, 47(10): 151-158. https://doi.org/10.13995/j.cnki.11-1802/ts.025943
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    In this study, the non-rinsed large yellow croaker surimi was used as raw material and was added 4 groups of additives including 6% starch + 0.5% NaHCO3, 6% starch + 0.5% Na4P2O7, and 6% soybean protein isolate (SPI)+0.5% NaHCO3 and 6% soy protein isolate +0.5% NaHCO3. Its gel strength, hardness, water holding capacity, cooking loss, whiteness, pH, water migration changes and dynamic rheological properties were measured. The results showed that, compared with the non-rinsing control group (ck), the addition of the additives in the 4 groups all enhanced their gel strength, texture profile analysis, water retention capacity, pH dynamic rheological properties G′, etc. to vary degrees. The addition of starch made gel strength of the Na4P2O7 group to the maximum value of 358.65 g·cm. Moreover, the starch and NaHCO3 group improved the gel hardness and water migration ability most significantly (P<0.05); the dynamic rheological results showed all 4 groups of additives could help increase the storage modulus (G′) of surimi gel. However, the addition of 4 groups of additives also reduced the whiteness of the gel to a certain extent. Comprehensive indicators showed that compared with soy protein isolate, adding starch could better improve the gel properties and water retention capacity of non-rinsed large yellow croaker surimi, while adding NaHCO3 and Na4P2O7 could improve the water retention capacity of the gel and the effect is equivalent.
  • ZHU Daoyang, WEI Chunyu, YU Xiaobin
    Food and Fermentation Industries. 2021, 47(10): 159-163. https://doi.org/10.13995/j.cnki.11-1802/ts.025998
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    This study established rapid high performance liquid chromatography method for the detection of pyrroloquinoline quinone (PQQ). In order to screen the strains that produce PQQ, and increase the fermentation yield of PQQ, a selective medium with methanol was adopted as the sole carbon source. Spectroscopy was used for preliminary screening of the samples, HPLC was used for re-screening, and the fermentation medium of the selected strains to produce PQQ was optimized. The results showed that 56 PQQ-producing strains were screened from the samples, and the highest yield was 20.6 mg/L. Through 16s rDNA sequencing analysis, the strain was identified as Methylopila sp.. The methanol content in the fermentation medium was also optimized. When the methanol volume concentration was 2.5%, the PQQ yield reached 26.3 mg/L, which was 28% higher than that in the initial medium with 1.0% methanol. The HPLC method established in this study provided a rapid detection method for the screening of PQQ high-yielding strains. The selected strains had high methanol tolerance and PQQ production potential, which could provide a reference for further PQQ fermentation research.
  • WAN Jinjuan, XIA Aijun, XUE Hui, SHAO Junjie, ZHANG Meiqin, SHEN Meifang, ZHAO Yanhua, SUN Mengling
    Food and Fermentation Industries. 2021, 47(10): 164-169. https://doi.org/10.13995/j.cnki.11-1802/ts.026091
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    In this study, the effects of 4, -20 and -80 ℃ storage conditions on the Hypophthalmichthys molitrix muscle quality and nutritional components were investigated. Water holding capacity, textural properties, conventional nutritive composition and amino acids content of Hypophthalmichthys molitrix were determined when stored at different temperature for 48 h. The results showed that, compared to fresh muscle, the drip loss, stored loss and frozen leakage rate of muscle stored at those three temperatures tended to increase; however, the hardness, springiness, chewiness and the content of moisture, crude protein, crude fat and total amino acids tended to decrease. Moreover, the drip loss of Hypophthalmichthys molitrix at -20 and -80 ℃ were significantly lower than of the sample at 4 ℃, while the content of crude protein, crude fat and total amino acids were significantly high (P<0.05). The stored loss and frozen leakage rate of muscle were significantly lower than that at 4 and -20 ℃, while the hardness and springiness were significantly high (P<0.05). And compared to lower storage temperature, the chewiness of muscle at 4 ℃ was significantly increased (P<0.05). In conclusion, the muscle quality and nutritional components of Hypophthalmichthys molitrix decreased after 48 h storage at 4, -20 and -80 ℃, and lower temperature (-20 and -80 ℃) storage is conducive to retard the quality deterioration.
  • SHU Na, WANG Bei, OUYANG Ke, MENG Qing, TONG Huarong
    Food and Fermentation Industries. 2021, 47(10): 170-177. https://doi.org/10.13995/j.cnki.11-1802/ts.026031
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    High-performance liquid chromatography (HPLC) and colorimeter were used to study the variation of main lipophilic pigments and the changes of color during the green tea manufactory process. Meanwhile, partial least squares regression (PLSR) was performed on the connection between the pigments and color quality of green tea. The results showed that the numbers of chlorophyll species increased obviously during the pan-fire period, while carotenoid species were decreased during baking. Furthermore, the total content of chlorophylls and carotenoids decreased during the green tea manufactory process. The main steps, in which chlorophyll a, chlorophyll b and carotenoids decomposed, pheophytins formed greatly, a* rose significantly, L* and b* decreased significantly, were pan-fire and first drying. The content of lipophilic pigments decreased continuously and the color became dark gradually during baking. Analysis of PLSR indicated that there were 15, 14 and 15 key lipophilic pigments that affected L*, a* and b*, respectively. The contents of chlorophyll a and chlorophyll b exhibited a significant positive correlation with L*, but had a significant negative correlation with a*. Moreover, the contents of pheophytin had a significant negative correlation with L*, but a significant positive correlation with a*. Reducing the damage of heat treatment to chlorophyll a and chlorophyll b in green tea processing can improve the color quality of dry tea.
  • WEN Anyan, QIN Likang, ZENG Haiying, ZHU Yi
    Food and Fermentation Industries. 2021, 47(10): 178-184. https://doi.org/10.13995/j.cnki.11-1802/ts.025754
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    Our previous study found that high-yield tetramethylpyrazine (2.97 mg/g DW) and fibrinolytic enzyme (720.84 U/g) were obtained in Bacillus subtilis BJ3-2-fermented polished adlay. Taking dehulled, polished and broken adlay as raw materials and B. subtilis BJ3-2 as fermentation strain, the fermentation conditions for high-yield tetramethylpyrazine and fibrinolytic enzyme production were optimized by single factor and Box-Behnken experiments in this study. Results showed that the tetramethylpyrazine yield and fibrinolytic enzyme activity of B. subtilis BJ3-2-fermented dehulled adlay were 6.15 mg/g DW and 2236.47 U/g when inoculation amount, fermentation temperature and fermentation time were 9.0%, 40 ℃ and 93 h. The tetramethylpyrazine yield and fibrinolytic enzyme activity of B. subtilis BJ3-2-fermented polished adlay were 6.94 mg/g DW and 2142.18 U/g when inoculation amount, fermentation temperature and fermentation time were 8.1%, 38 ℃ and 96 h. The tetramethylpyrazine yield and fibrinolytic enzyme activity in B. subtilis BJ3-2-fermented broken adlay were low even after optimization. Additionally, the tetramethylpyrazine yield of B. subtilis BJ3-2-fermented adlay was higher than that of B. subtilis BJ3-2-fermented soybean and CICC 20637-fermented adlay. Therefore, considering the processing cost of adlay, B. subtilis-fermented dehulled adlay could be the optimal fermentation system to obtain high-yield tetramethylpyrazine and fibrinolytic enzyme.
  • WANG Zichao, GUO Jiayuan, GAO Wenshuo, CUI Jingwen, YANG Qingqing, ZHU Jinfan, ZHANG Huiru
    Food and Fermentation Industries. 2021, 47(10): 185-191. https://doi.org/10.13995/j.cnki.11-1802/ts.025890
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    Due to the serious resource waste and environmental pollution induced by wheat straw in China, wheat straw was used by Chaetomium globosum CGMCC 6882 to produce exopolysaccharide in present work. Meanwhile, the structure of the exopolysaccharide and its antioxidant activity were investigated. Results showed that monosaccharide composition of the polysaccharide produced by C. globosum CGMCC 6882 was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46∶1.58∶1.11∶55.15∶36.37∶7.04∶7.34, and its molecular weight was 3.538×104 Da. Antioxidant assays in vitro revealed that the polysaccharide had strong scavenging effects on DPPH radical, hydroxyl radical, superoxide anions and ABTS radical in a dose-dependent manner. At the concentration of 5 mg/mL, the corresponding scavenging abilities of the polysaccharide were (83.08±2.58)%, (81.39±1.47)%, (79.38±2.03)% and (85.69±1.62)%, respectively. Results of cell antioxidant test showed that the activity of MDA decreased from (15.8±0.25) μmol/g to (7.5±0.31) μmol/g, which was negatively correlated with the concentration of polysaccharide. Furthermore, the activity levels of SOD, CAT and GSH-Px in H2O2 induced Caco-2 cells increased from (147.28±4.82), (11±1.15) and (18.08±1.96) U/mg to (238.89±3.91), (39.12±0.76) and (41.35±2.18) U/mg, and those were positively correlated with the concentration of polysaccharide. Overall, this work provides an important reference and theoretical basis for the efficient utilization of wheat straw.
  • LI Jianhua, WANG Fengcheng, WEI Xue, HAN Wenyan, QIAO Jiajia
    Food and Fermentation Industries. 2021, 47(10): 192-197. https://doi.org/10.13995/j.cnki.11-1802/ts.025764
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    Two kinds of wheat flour with wet gluten content of 20% and 36% were formulated into nine wheat flours with different wet gluten contents to study the effects of physicochemical properties, farinograph characteristics and tensile properties of these flours on the quality of northern-style steamed stuffed bun (Baozi). The results showed that the farinograph characteristics and tensile properties changed significantly with the increase of wet gluten content. The specific volume of Baozi increased first and then decreased and the whiteness gradually decreased with the increase of wet gluten content. The increase in dough water absorption, formation time, stabilization time, and farinograph quality index promoted the increase of the specific volume of Baozi when wet gluten content was increased from 20% to 32%. Besides, the degree of softening was significantly negatively correlated with the specific volume of Baozi and the stretched area was positively correlated with the specific volume of Baozi at a wet gluten content of 20%-32%. The specific volume of Baozi reached the maximum, the elasticity and cohesion were high and the sensory scores were all above 85 at a wet gluten content of 28%-32%. The sensory score reached the highest at a wet gluten content of 30%. Therefore, wheat flour with a wet gluten content of 28%-32% is suitable for making high-quality northern-style steamed stuffed bun.
  • GUO Lipan, ZHANG Lu, DUAN Xiaoting, WANG Ronghui, XU Xiao
    Food and Fermentation Industries. 2021, 47(10): 198-202. https://doi.org/10.13995/j.cnki.11-1802/ts.026099
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    A method for determination of 15 kinds of elements of K, Na, Ca, Mg, P, Fe, Mn, Cu, Zn, Al, As, Pb, Cd, Cr, Se in oyster sauce by inductively coupled plasma optical emission spectrometry/inductively coupled plasma mass spectrometry (ICP-OES/ICP-MS) was established, and the nutritive value and possible risks of health and safety were assessed. The samples were digested by nitric acid and hydrogen peroxide. The spectral interference of ICP-OES was eliminated by selecting different analysis lines, and the matrix effect of ICP-MS was diminished by adding internal standard elements of Ge, Rh, and Re online, and the mass spectral interference was diminished by kinetic energy discrimination (KED). In addition, scallop reference material (GBW 10024) was used to verify the accuracy of the method. The results showed that the detection limits of ICP-OES were 0.125-0.987 mg/kg with 89.3%-103.5% the average recoveries, and the relative standard deviations (RSD) were 0.2%-3.7%. While the detection limits of ICP-MS were 0.001-0.014 mg/kg, the average recoveries were 87.2%-107.8% and the RSD was 0.2%-2.7%. The linear relationship of standard curves was good (R2>0.999). The contents of macro-elements of K, Na, Ca, Mg in the oyster sauce were high, while heavy metals of As, Pb, Cd, Cr were low. This method is accurate, efficient and sensitive. It can meet the requirements for the determination of elements in oyster sauce.
  • GUO Ting, CHEN Jinhang, ZHOU Hongyuan, ZHANG Yuhao, MA Liang
    Food and Fermentation Industries. 2021, 47(10): 203-206. https://doi.org/10.13995/j.cnki.11-1802/ts.025904
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    Zearalenone (ZEN) is a kind of toxin produced by Fusarium fungi. It has reproductive toxicity, immunotoxicity, hepatorenal toxicity and carcinogenicity, and poses a serious threat to human health. In this work, a facile and sensitive fluorescence sensing system was developed for the detection of ZEN. Aptamer as recognition element could recognize the target ZEN. Genefinder was chosen as the sensing signal, the fluorescence signal will increase due to the interaction between Genefinder and double DNA which formed by ZEN aptamer and its complementary strand. But in the presence of ZEN, the aptamer could recognize the ZEN, leading to decrease of fluorescence. The results showed that the sensing system was high selectivity for ZEN, and the detection linear range was 0.1-200 μg/L with 0.1 μg/L the detection limit under the optimized condition. For real corn flour sample, the standard recovery ranges from 100.4% to 105.8%. The fluorescence sensing system has been successfully used for the detection of ZEN in the real sample which showed a wide application potential.
  • YANG Fang, FAN Chengmeng, JIA Hongfeng, REN Fang
    Food and Fermentation Industries. 2021, 47(10): 207-213. https://doi.org/10.13995/j.cnki.11-1802/ts.026579
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    The differences of physical and chemical properties and flavor were studied in Morchella spp. samples from four habitats,including Changbaishan, Linzhi, Qingchuan and Shangri-la. The results showed that the contents of protein, crude polysaccharide, and total phenol in Linzhi Morchella spp. were higher than that in the other three habitats, and the content of protein was significantly different (P <0.05). Seventeen kinds of amino acids, including 7 kinds of essential amino acids, were detected in 4 samples. Glutamic acid, alanine, and arginine were significant differences (P <0.05) and with the highest content in umami, sweet and bitter flavors respectively. The total amount of free amino acids and total amount of essential amino acids in Changbaishan Morchella spp. was the highest. Moreover, the results of gas chromatography-ion mobility spectrometry on the detection of volatile flavor compounds in each sample showed that the characteristic peaks of flavor compounds in Changbaishan and Linzhi Morchella spp. were quite different from those in Morchella spp. from other habitats. The characteristic flavor compounds of Linzhi Morchella spp. mainly included 2-butanone, iso-butanol, etc.; the characteristic flavor compounds of Shangri-la Morchella spp. mainly included γ-butyrolactoneetc; Qingchuan Morchella spp. had similar flavors to Shangri-la Morchella spp. Furthermore, the results of principal component analysis showed that the sum of PC_1 [43%] and PC_2 [33%] was 76%, which could be used to distinguish Morchella spp. from different habitats well. The results of heat map cluster analysis were consistent with the results of PCA analysis. Therefore, it is feasible to analyze the flavor compounds of Morchella spp. based on GC-IMS and distinguish Morchella spp. from different habitats.
  • REN Chunchun, JIA Yulong, LOU Yilong, QIAO Shiyu, XU Wenjing, QIN Likang
    Food and Fermentation Industries. 2021, 47(10): 214-221. https://doi.org/10.13995/j.cnki.11-1802/ts.026330
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    A comparative analysis of the main nutrients including dietary fiber, protein, free amino acids, total sugar, crude fat, tannin, minerals and functional components of polyphenols, flavonoids, polysaccharide, phytosterols and γ-aminobutyric acid of the square bamboo shoots from different regions in Guizhou. And principal component analysis was used to comprehensively evaluate its quality. The results showed that in the 9 fresh bamboo shoot tested samples, the dietary fiber, protein and total free amino acid (TFAA) content content were 17.87-38.11 g/100g DW, 32.225-42.30 g/100g DW and 821.27-568 3.72 mg/100g DW respectively. Moreover, the highest content of essential free amino acid (EAA) was Q-5 (205 8.74 mg/100g DW) and the best ratio of EAA/TFAA was Q-1 (37.20%). For minerals, potassium was the highest ( 3 571.43-9 620.00 mg/100g DW). Besides, polyphenol, flavonoid, Phytosterols like β-sitosterol and stigmasterol content were 602.83-854.07 mg/100g DW, 299.35-638.86 mg/100g DW,208.99-306.77 and 81.89-130.36 mg/100g DW respectively. And γ-aminobutyric acid content was 70.06-704.94 mg/100g DW. Furthermore, Q-1 was significantly higher than other fresh bamboo shoots (P<0.05). The polysaccharide content was 817.93-179 8.31 mg/100g DW. By using PCA analysis, six principal components (water, β-sitosterol, γ-aminobutyric acid , flavonoids, polysaccharides, Ca) were proposed and the cumulative variance contribution rate was 93.377%. The top three bamboo shoots in terms of quality were Q-1, Q -6 and Q-3. The square bamboo shoots have great development potential because they were rich in nutrients and functional ingredients.
  • TANG Shijie, YIN Mingyu, RYOSUKE Matsuoka, XI Yinci, WANG Xichang
    Food and Fermentation Industries. 2021, 47(10): 222-228. https://doi.org/10.13995/j.cnki.11-1802/ts.025841
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    This study aimed to evaluate the comprehensive quality of 6 kinds of salad dressings, which named BL、HS、HM、QB、KF、WM, sold in the market by principal component analysis and cluster analysis. Six kinds of salad dressing samples were analyzed by free amino acid analyzer under optimized conditions. There were 17 kinds of free amino acids were detected with the average total amount of 1 155.54 mg/100g. There were significant differences in the contents of free amino acids, total essential amino acids, taste-active amino acids and limiting amino acids. QB contained taste-active amino acids and essential amino acids, and WM contained more limiting amino acids. Glutamic acid contributed the most to the taste characteristics with TAV(Taste activity values)values ranged from 17.35 to 7 878.75. Two principal components were extracted by principal component analysis, and the cumulative variance contribution rate was 94.28% which indicated the effect of free amino acids on different salad dressings. And the highest comprehensive score was WM. Moreover, the results of cluster analysis showed that the 6 kinds of salad dressings could be divided into three groups which showed the same trends in principal component analysis. So, principal component analysis and cluster analysis can better analyze the composition of free amino acids in salad dressing, which can be used as the quality evaluation index of salad dressing. High or low free amino acid content will reduce the quality of salad dressing. The best quality salad dressing in this study is WM.
  • SUN Qing, XU Wenhua, ZHOU Huakun
    Food and Fermentation Industries. 2021, 47(10): 229-234. https://doi.org/10.13995/j.cnki.11-1802/ts.025851
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    The aim of this study was to evaluate the potential application value of the berry resources of ribes genus including wild Ribes himalense, Ribes qingzangense, Ribes menyuanense, and Ribes alpestre. The methods in the national food safety standards and the laboratory self-built methods were used to determine and comprehensively analyze the general nutrients, vitamins, amino acids, minerals, heavy metals, and biologically active components of the fruits. The results showed that the nutrient content of ribes fruit had low fat (1.83±0.39)%, high VC (58.20±8.77) mg/100g, high VE (1.53±1.28) mg/100g, high potassium (271.50±64.14) mg/100g and other characteristics, which could be used to develop new food sources. The active ingredients detected in the fruit were total flavonoids (0.18±0.11)%, polyphenols (0.22±0.20)%, polysaccharides (3.34±3.09)%, anthocyanins (0.09±0.07)%, etc. It provides a theoretical basis for the future development and utilization of Ribes berry resources.
  • LIANG Guangyan, JIANG Yangming, ZHOU Mei, XIA Yu, LI Chunyan, HU Enming, YAN Qiuxiao, WANG Daoping
    Food and Fermentation Industries. 2021, 47(10): 235-239. https://doi.org/10.13995/j.cnki.11-1802/ts.025926
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    A quantitative analysis using q1H-NMR was developed for the determination of ethanol and acetic acid in fermented drinks. The NMR experimental conditions were as follows: using DMSO-d6 as solvent, TMSP as internal standard, 90° pulse, scanning times NS (16), relaxation time D1 (20 s), sampling time AQ (4.5 s), constant temperature test at 298 K. Using ethanol (1.10 ppm), acetate acid (1.80 ppm) and TMSP (0.00 ppm) peaks as quantitative peaks, the content of ethanol and acetic acid in five fermented drinks were determined. The linear range and recovery rate of ethanol was 64.05-0.32 mg/mL and 99.68%-100.65%, and those for acetate acid was 19.51-0.20 mg/mL and 98.38%-99.24%. The repeatability RSD of ethanol and acetic acid were 0.11% and 0.99% (n=6), respectively, and the test results were accurate and reliable within 48 hours. The high universality, accuracy, reproducibility, and efficiency of the q1H-NMR method demonstrated in this work suggest that this method could serve as a highly potential quantification alternative for quality assessment of alcohols and acids.
  • SI Bo, YUAN Wenwen, GU Huihui, JIA Mengwei, LU Yongling, LYU Lishuang
    Food and Fermentation Industries. 2021, 47(10): 240-245. https://doi.org/10.13995/j.cnki.11-1802/ts.025794
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    Reactive carbonyl species (RCS) are toxic and highly reactive materials in liquor fermentation process which bring potential safety hazards to liquor products. In this study, a high performance liquid chromatography analysis method was established to simultaneously determine acrolein, methylglyoxal, crotonaldehyde, 5-hydroxymethylfurfural, formaldehyde and acetaldehyde in Chinese Baijiu. A comprehensive analysis of different flavor liquor samples and wine samples during processing was carried out. The diluted sample was derivatized with 2, 4-dinitrobenzene hydrazine at 50 ℃ for 1.5 h. A Beconsil C18 chromatographic column (250mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile and water under gradient elution at a flow rate of 1.0 mL/min. The column temperature was set to 40 ℃, and the diode array detector was selected as dual-channel detection, with detection wavelength of 360 nm (ACR, MGO, CRO, FOR, ACE) and 400 nm (5-HMF), respectively. This method exhibited good linearity with correlation coefficients all above 0.999. The detection limits were in the range of 1-8 ng/mL and the quantitate on limit were in the range of 3-25 ng/mL. The spiked recoveries ranged from 85.39%-105.78%, with RSDs less than 4%. The level of RCS in Maotai-flavor Baijiu and sesame-flavor Baijiu was higher, which was speculated to be related to the high temperature fermentation. In the process of 8 rounds of fermentation and 7 times of liquor extraction, the RCS concentration in Maotai-flavor Baijiu was the highest in the 3rd, 4th and 5th rounds. The RCS of strong-flavor liquor rose to the highest in the process of “accumulation out of cellar”, and the RCS level in the sample of “the double bottom of liquid fermentation” and “the middle layer of liquid fermentation” were ranked first and second, respectively. Our method is stable and sensitive, which is easy to operate, and suitable for detecting RCS in liquor and semi-finished products in fermentation processing.
  • WANG Yan, LI Demei, SUN Zhiwen, WANG Zongyi
    Food and Fermentation Industries. 2021, 47(10): 246-250. https://doi.org/10.13995/j.cnki.11-1802/ts.025728
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    In order to detect the content of several phenolic substances that affect the bitterness and astringency of cabernet Sauvignon dry red wine, five flavane-3-alcohol monomers and two proanthocyanidin dimers in wine were measured by HPLC(Shimadzu LC-20AD) after extraction with ethyl acetate. The detection conditions were as follows: chromatogram column: Platisil ODS(250 mm×4 mm,5 μm), flow rate 1.0 mL/min, column temperature 30℃, wavelength 280 nm, gradient elute, solvent A: V(water)∶V(acetic acid)=98∶2, solvent B: acetonitrile. The concentration of these seven components had good linear relationships with their peak areas within a certain range, the correlation coefficient was between 0.997 3-0.999 9, the detection limit was between 0.008 3-0.187 5 mg/L, and the average recovery was between 91.12%-108.84%. The relative standard deviation of the retention time of standard and sample was within 1%, and that of the peak area was within 4%. Seven phenolic substances were detected in all dry red wines from five different seed regions of the Eastern Foot of Helan Mountain in Ningxia, epigallocatechin was not detected in some wine samples. The contents of epicatechin and procyanidin B1 were higher than other substances. The highest total phenol content was 237.528 mg/L, and the lowest was 127.237 mg/L.
  • HUANG Chen, XIE Jing, XUE Bin, SHAO Zehuai, GAN Jianhong, LI Xiaohui, SUN Tao
    Food and Fermentation Industries. 2021, 47(10): 251-258. https://doi.org/10.13995/j.cnki.11-1802/ts.025781
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    The physical properties, biological activities and preservation applications of soybean polysaccharide film was reviewed. Firstly, the effects of modifier, such as nanoparticles, essential oils, gelatin, on the physical properties of soybean polysaccharides film were discussed. Furthermore, the effects of natural bacteriostatic agents and nanoparticles on the antimicrobial and antioxidant properties of the film were reviewed. Finally, the application of soybean polysaccharide film in the food preservation was summarized. The work will provide some reference for the research of soybean polysaccharide film.
  • LAN Weiqing, ZHAO Xinyu, LIU Jiali, MEI Jun, XIE Jing
    Food and Fermentation Industries. 2021, 47(10): 259-264. https://doi.org/10.13995/j.cnki.11-1802/ts.025500
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    Plant polyphenols are plant-specific secondary metabolites, which mainly presented in the tissues and organs of plants such as fruits, skins, roots and leaves. Plant polyphenols have antioxidative, free radicals scavenging, antibacterial, anti-inflammatory, antiviral, anti-cancer and anti-cardio-cerebrovascular diseases activities. Because of its rich sources, natural ingredients and diverse effects, the research on plant polyphenols has attracted widespread attention. Based on the comparison of main advantages and disadvantages of common preservation methods in aquatic products, the advantages of biological preservation were clarified in this paper, the main antimicrobial mechanism of plant polyphenols in plant-derived biological preservatives and the research progress of their application in the preservation of aquatic products were reviewed, the existing problems and solutions were proposed. The developmental trend of plant polyphenols was also put forward.
  • LIU Weiwei, WANG Hongxia, ZHANG Yuhao, MA Liang, DU Jie, PENG Lin
    Food and Fermentation Industries. 2021, 47(10): 265-270. https://doi.org/10.13995/j.cnki.11-1802/ts.025997
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    With consumers′ attention to health and environmental protection, especially for inks applying into food fields, the study of green, environmentally-friendly, non-toxic inks is emerging. Gelatin has good physical and chemical properties including edibility, degradability, viscous property and film-forming property, and it is widely used in various fields including food, agriculture, industry, which can be used to prepare gelatin-based inks. This paper discussed the progress of gelatin-pigment (red, yellow, blue, white, black, etc.) composite, summarized the application of gelatin based ink in food decoration and electronic products (based on food), and pointed out the development direction of gelatin in environmentally-friendly ink, hoping to provide the corresponding theoretical basis for gelatin based ink in food.
  • LI Hui, BAO Hairong
    Food and Fermentation Industries. 2021, 47(10): 271-277. https://doi.org/10.13995/j.cnki.11-1802/ts.025839
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    The natural polysaccharide is a kind of biopreservative with abundant sources and low prices. It has various biological activities such as antibacterial and anti-oxidation, no residue, no pollution and high safety. It has broad application aspects in the field of aquatic product preservation. This paper reviewed the forms of natural polysaccharides used in the refrigeration and preservation of aquatic products, their fresh-keeping mechanisms and various application patterns. The existing problems were also been summarized, and natural polysaccharide preservatives could be compounded with other preservation technologies in the future were proposed. This paper aimed to provide a reference for the practical application of natural polysaccharide preservatives in the refrigeration and preservation of aquatic products.
  • HU Xiaomin, HUANG Peng, LIU Wenxin, WU Xinye, JING Xianyu, QIN Wen
    Food and Fermentation Industries. 2021, 47(10): 278-284. https://doi.org/10.13995/j.cnki.11-1802/ts.026062
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    Fresh-cut fruit and vegetables, as a kind of fresh, convenient, nutritious and healthy food, are favored by consumers. However, processing could destroy the complete organizational structure of fruit and vegetables, easily lead to enzymatic browning, microbial pollution and other adverse effects, thus accelerating the deterioration of quality, shortening the shelf-life of products and seriously affecting their commercial value. Therefore, it is imperative to preserve fresh-cut fruit and vegetables. Non-thermal physical technologies have been widely used in fresh-cut fruit and vegetables due to their advantages of simplicity, efficiency, environmental protection, safety and pollution-free. It has attracted much attention and recognition at home and abroad. This paper reviewed the preservation principle, advantages and application effects of six kinds of novel non-thermal physical technologies in fresh-cut fruit and vegetables. The possible mechanisms of these techniques in killing microorganisms, inhibiting enzymatic browning and promoting secondary metabolism were analyzed. The problems existing in the practical application of these techniques were summarized. In addition, the most suitable application level of each technology is compared and analyzed. The aim of this review is to provide reference for scientific research and industrial production.
  • MA Jing, SUN Lu
    Food and Fermentation Industries. 2021, 47(10): 285-290. https://doi.org/10.13995/j.cnki.11-1802/ts.026655
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    Yak milk and its products (yogurt, Qula, butter, etc.) are precious high-end resources on the Qinghai-Tibet Plateau. As an important part of the Tibetan diet, it is suitable for people of all ages. Its chemical composition and nutritional composition are hotspots. Protein is a key factor with multiple functions in milk. With the development of bioinformatics technology, relying on proteomics technology, the work of studying the composition, molecular structure and biological activity function of milk and product protein is gradually deepening. This article reviewed and discussed the current development of proteomics technology, the application of protein composition and the research of proteomics technique in yak milk.
  • WU Renzhi, HU Xinjie, HAN Guoquan, YI Yan, SHU Jiaxin, CAO Yang, YU Dongmei, ZHAO Junmei, ZHANG Yi, ZHANG Yuwei
    Food and Fermentation Industries. 2021, 47(10): 291-296. https://doi.org/10.13995/j.cnki.11-1802/ts.025849
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    Staphylococcus aureus is one of the most common pathogens causing food poisoning, early and rapid detection of food contamination is the key to food safety and quality control. Traditional detection technologies are complicated and time-consuming, this article introduces current applications from the aspects of physiological and biochemical technology, immunoassay technology, molecular biology technology, protein-based detection technology and biosensors, etc. The rapid detection technology is expected to provide ideas for the selection and development of food-borne S. aureus rapid detection methods.
  • TAN Fengling, CHU Ruichao, SI Mengya, WANG Baoshi, ZHANG Mingxia
    Food and Fermentation Industries. 2021, 47(10): 297-303. https://doi.org/10.13995/j.cnki.11-1802/ts.025786
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    Trace amounts of volatile sulfide compounds (VSCs), such as mercaptan, hydrogen sulfide, thioether, mercaptan ester, sulfur-containing fuel oil, and heterocyclic compounds, are found in wine. These VSCs have an important influence on the aroma, color, and taste of wines. This paper systematically analyzes the latest research progress of VSCs in wine from the perspective of source, sensory characteristics and analysis methods. Recently, gas chromatography-mass spectrometry and high-performance liquid chromatography-mass spectrometry have played an important role in the detection of VSCs. However, due to the complexity of wine substrates, the difficulties of VSCs detection, such as high volatility, poor stability and low detection threshold, so it is urgent to develop efficient and convenient detection methods for VSCs in complex samples. This review aims to provide reference for the effective control and regulation of VSCs in wine and the development of high-quality wines.
  • MAO Bifei, ZHANG Bingcong
    Food and Fermentation Industries. 2021, 47(10): 304-308. https://doi.org/10.13995/j.cnki.11-1802/ts.026530
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    In order to improve the ideological and political quality of college students majoring in food and cultivate high-quality personnel, this paper studies the ideological and political teaching reform mode of Food Biotechnology course under the background of innovation and entrepreneurship. The traditional "Classroom Instillation" single ideological and political mode was reformed, and a new curriculum ideological and political mode of "Classroom-Experiment-Practice" was constructed. By combining classroom ideological and political education with experiment and practice, it can effectively enhance the effect of ideological and political education, improve the teaching quality and promote innovation and entrepreneurship, and thereby realize the organic unity of value guidance, knowledge education and ability training, and finally serve the country and society with practical personnel.