25 March 2022, Volume 48 Issue 6
    

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  • LI Yangyang, LIU Song, YIN Xiaoqiang, DU Guocheng
    Food and Fermentation Industries. 2022, 48(6): 1-7. https://doi.org/10.13995/j.cnki.11-1802/ts.028442
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    Xylanase catalyzes the decomposition of xylan into xylooligosaccharides and xylomaccharides, which is of great significance for the efficient utilization of xylan. In this study, secretory expression of β-D-1,4 endoxylanase (xynA) from Aspergillus niger AG11 was achieved in Escherichia coli BL21 (DE3). And the optimal expression conditions of xynA were determined as follows: the concentration of inducer (isopropylthiogalactoside) 0.1 mmol/L, induction temperature of 20℃, and induction initiation OD600 of 0.5. Under these conditions, the extracellular xynA activity reached 15.8 U/mL, 85.9% higher than that of the initial condition. The recombinant xynA was purified to a single band by nickel column and its enzymatic properties were analyzed. It was showed that the specific enzyme activity, optimum reaction temperature, and pH were 175.1 U/mg, 50 ℃ and 4.0, respectively. The half-life of recombinant xynA was 182 min at 40 ℃, and the activity of recombinant xynA remained above 70% when incubated at pH 2.0-9.0 for 1 h. Using birchwood xylan as a substrate, the Km and kcat values of recombinant xynA were 3.45 g/L and 58.51 s-1, respectively. The results lay a foundation for the screening of excellent mutants and industrial applications.
  • YANG Guoshuai, XU Ying, ZHAN Xiaobei, JIANG Yun, LI Zhitao, GAO Minjie
    Food and Fermentation Industries. 2022, 48(6): 8-14. https://doi.org/10.13995/j.cnki.11-1802/ts.028973
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    Low-molecular-weight xanthan gum (LMWXG) has biological activities such as anti-oxidation, and anti-bacterium, it can be applied in food and agriculture fields. Endoxanthanase with high-level expression is a prerequisite for enzymatic preparation of LMWXG. The endoxanthanase gene from Microbacterium sp. XT11 was expressed in Pichia pastoris GS115 for the first time. The cultivation conditions of the recombinant Pichia pastoris were optimized. The enzymatic properties of the endoxanthanase were also studied. The optimal fermentation conditions for the recombinant P. pastoris with GAP promoter were 30 ℃, pH 6.0, 220 r/min, and 5% inoculation. Under these conditions, the maximum activity of the recombinant endoxanthanase reached 1 230 U/L in a 7-L bioreactor. The enzyme was relatively stable at pH 5.5-7.5 and 20-45 ℃. Its optimal pH and temperature were 6.0 and 40 ℃, respectively. It could directly hydrolyze the main chain of xanthan gum to form LMWXG (1 400 Da). Therefore, this study may provide a feasible way for the industrial production of LMWXG.
  • WU Juandi, ZHANG Bo, HAN Liting, YANG Bo, WANG Yingjie, HAN Shunyu, CHEN Jianjun, ZHANG Junqiang, ZHANG Zhulin
    Food and Fermentation Industries. 2022, 48(6): 15-24. https://doi.org/10.13995/j.cnki.11-1802/ts.028158
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    To explore the effect of metal ion, metal ion concentration and phenolic acid treatment on the pigment-metal complex in wines, K+, Mg2+ and Al3+ were assessed in simulated wine solutions by ultraviolet spectrophotometry and CIELab method. The results showed that the addition of Mg2+ and Al3+ copigments was beneficial to obtain darer light (L*), redder (a*) and more saturated (C*ab) colors with the increase of metal ion concentrations. The Al3+ would lead to a noticeable color difference at high concentration (>1∶50 mol/L). In contrast, the K+ treatment showed a poor copigmentation ability. The copigmentation effect of pigment-K+/Al3+complexes could be enhanced by vanillic and protocatechuic acids addition, but these phenolics could reduce the effect of Mg2+ treatment, leading an anti-copigmentation phenomenon. In addition, compared with the protocatechuic acid, the effect of vanillic acid was superior. The results also showed that these three metal ions could demonstrate anti-copigmentation at lower concentration of phenolic acids. However, with the increase of phenolic acid concentration, the effect of these three kinds of metal ions gradually appeared, and had a positive impact on the color of simulated wine. These results provide data support for the study of the color reaction of pigment-metal complex and their role in improving wine color quality.
  • TANG Li, WEI Wenli, ZHAO Yajiao, YU Wenping, WU Zhengyun, ZENG Li, ZHANG Wenxue
    Food and Fermentation Industries. 2022, 48(6): 25-31. https://doi.org/10.13995/j.cnki.11-1802/ts.028275
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    High-throughput sequencing was used to analyze the diversity and succession of fungal community in the fermentation process of industrial radish Paocai in two different factories (J and W), and the dynamic changes of physicochemical properties were detected, and redundancy analysis (RDA) was performed. According to the characteristics of fungal community structure, the whole fermentation process can be divided into initial point (0 d), early stage (30-60 d), middle stage (60-180 d) and late stage (180-240 d) according to the principal component analysis. The dominant phylum was Ascomycota, followed by Basidiomycota. At genus level, the relative abundance of Candida decreased first and then increased during the fermentation of radish Paocai in both plants. Debaryomyces was the dominant genus in the early stage and Starmerella was the dominant genus in the middle stage of fermentation. Pichia was the dominant fungus genus peculiar to the 0 d fermentation in J plant, and Sterigmatomyces and Tausonia were the dominant fungus genus peculiar to the 0 d and 30 d radishes in W plant, respectively. RDA showed that total acid and pH had significant effects on the fungal community structure of radish Paocai fermentation in plant J, while tartaric acid was the main factor driving the change of the fungal community structure of radish Paocai in plant W. This result provides a theoretical basis for the quality control of Sichuan radish Paocai and the optimization of fermentation process.
  • YE Chen, ZHANG Bao, YAO Xi, XUN Hang, TANG Feng
    Food and Fermentation Industries. 2022, 48(6): 32-38. https://doi.org/10.13995/j.cnki.11-1802/ts.028325
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    Variation of 17 mineral elements in the aging process of bamboo liquor was studied using ICP-MS. Results showed that the aging process significantly affected the content of mineral elements in bamboo liquor. At the beginning of aging, potassium content increased exponentially, reaching a peak of 330.83 mg/L at 14th day, then gradually decreased, and stabilized at about 190 mg/L after 28th day. The content changes of calcium, sodium, chromium and arsenic followed to the first-order kinetic equation, and the half-lives were 26.05 days, 9.40 days, 9.59 days and 11.92 days, respectively. Exogenous flavonoids affected the variation of mineral elements in bamboo liquor to different degrees. Treatment with 2.5 mg/mL exogenous flavonoids significantly increased the peak value of potassium content, reaching 1 044.90 mg/L at 14th day, which was 3.16 times higher than that without addition. Under the treatment of 10 mg/mL, the potassium content increased fastest and reached the peak at 10th day. Arsenic content raised with the increase of exogenous flavonoids addition, while chromium content showed an opposite trend. Except for cobalt and molybdenum, 15 mineral elements were detected in bamboo liquor, and all the contents met the requirements of relevant food limit standards.
  • WANG Ting, YUN Jianmin, BI Yang, AI Duiyuan, ZHAO Fengyun, ZHANG Wenwei
    Food and Fermentation Industries. 2022, 48(6): 39-45. https://doi.org/10.13995/j.cnki.11-1802/ts.027524
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    To explore the change rule of flavor in the process of quality deterioration of Agaricus bisporus after harvest, headspace solid-phase micro extraction-gas chromatography-mass spectrometry technology combined with relative odor activity value (ROAV) and principal component analysis (PCA) methods were used to dynamic analysis of volatile flavor compounds in the process of postharvest quality deterioration of A. bisporus. In this way, the key volatile flavor substances and characteristic flavor substances were identified, and the changes of free amino acids, flavoring nucleotides, organic acids and other flavoring substances were dynamically analyzed by high-performance liquid chromatography-mass spectrometry technology. The results showed that in the process of postharvest quality deterioration of A. bisporus, the content of various volatile substances showed a certain change pattern, and the total content of volatile substances showed a trend of first decreasing and then increasing. ROAV analysis showed that 1-octene-3-ol, 1-octene-3-one, and 3-octanone were characteristic flavor substances in the process of quality deterioration of A. bisporus after harvest. PCA analysis showed that alcohols and aldehydes were flavors had the greatest impact; various flavoring substances also showed certain changes: the content of free amino acids and organic acids first increased and then decreased, and the content of flavor nucleotides slowly decreased. According to the change law of flavor components, the quality deterioration process of A. bisporus after harvest could be effectively distinguished, and provide a certain theoretical basis for the rapid quality detection, flavor identification and shelf-life prediction of A. bisporus.
  • JIANG Bo, HUANG Jianhua
    Food and Fermentation Industries. 2022, 48(6): 46-51. https://doi.org/10.13995/j.cnki.11-1802/ts.027925
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    The aim of this study was to explore the content characteristics of nutrient elements in tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) and establish the relationship between nutrient elements and producing area. Tumorous stem mustard samples were digested by microwave system, and the major nutrient elements (Mg, P, S, Ca, K) and trace nutrient elements (B, Mo, Mn, Fe, Cu, Zn, Sr) were simultaneously analyzed in the sample solution by inductively coupled plasma optical emission spectrometry (ICP-OES). After the measurement data were standardized, the principal component analysis was used to extract the principal factors to identify the characteristic elements of tumorous stem mustard. Cluster analysis was used to establish a discriminant model to trace the origin of tumorous stem mustard in different regions. The results showed that the cumulative variance contribution rate of the five principal components extracted by principal component analysis was 85.971%. Factor analysis identified Mg, P, S, K, B, Mn, Zn, and Cu as the characteristic elements of tumorous stem mustard. Cluster analysis showed that 16 tumorous stem mustard samples could be classified into three groups. The contents of nutrient elements in different samples have obvious regional distribution characteristics, which can be used for the identification of the origin of tumorous stem mustard. This research could provide a scientific theoretical basis for the quality evaluation and origin traceability of tumorous stem mustard.
  • CUI Ning, LIU Xiaolan, LI Guanlong, ZHENG Xiqun
    Food and Fermentation Industries. 2022, 48(6): 52-57. https://doi.org/10.13995/j.cnki.11-1802/ts.028001
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    Corn glutenin hydrolysate (CGH) was prepared by hydrolyzing corn glutenin with protamex and alcalase. The antioxidant activity of CGH in vitro was studied. The oxidative damage model of LO2 cells induced by ethanol was used to study the antioxidant stress of CGH. The results showed that CGH had good antioxidant activity in vitro. When the protein concentration was 2 mg/mL, the scavenging rates of ·OH and DPPH free radical were (86.69±0.022)% and (60.56±0.369)%, respectively, and the chelating capacity of Fe2+ was (91.58±0.025)%.CGH significantly decreased (P<0.05) the content of reactive oxygen species (ROS) in LO2 cells injured by ethanol, and significantly increased (P<0.05) the activities of superoxide dismutase (SOD) and glutathione reductase (GR), and the level of reduced glutathione (GSH) in LO2 cells injured by oxidative stress. This study laid a theoretical foundation for the application of CGH as a natural antioxidant in food industry.
  • ZHENG Yuqian, YU Yongxin, ZHAO Haixia, TANG Xiao, ZHANG Huaqing, CHEN Shu
    Food and Fermentation Industries. 2022, 48(6): 58-63. https://doi.org/10.13995/j.cnki.11-1802/ts.028214
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    16S rDNA amplification sequencing was applied to study the bacterial diversity of low-temperature yogurt in Shanghai supermarkets. A total of 16 yogurt samples were collected from local supermarkets, of which eight samples were from the same brand, and the rest were from eight different brands. Sixteen main bacterial operational taxonomic units (OTUs) sequences were identified from these samples, among them, the dominant OTUs were lactic acid bacteria from the starter cultures. In the meantime, seven other bacteria strains from the environment were also detected. The possible contamination sources were analyzed in the producing process, including raw milk collection, fermentation, packaging, storage and transportation. The results could provide theoretical basis for risk prevention and quality control of commercial yogurt.
  • ZENG Xianchun, ZHU Yaqin, WANG Mingming, LI Jiawen, LIU Manqiu, ZHUANG Weiwei
    Food and Fermentation Industries. 2022, 48(6): 64-70. https://doi.org/10.13995/j.cnki.11-1802/ts.028017
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    The impact of different Lactobacillus casei doses on the intestinal microbiota in mice was evaluated in this experiment. Seventy-two mice weighing (20±2) g were selected and randomly divided into six groups: normal control group, model control group, positive drug control group, L. casei low-dose group, medium-dose group and high-dose group, respectively. Mice were fed with compound diphenoxylate to establish a constipation model. Mice in the normal control group were fed with saline, and mice in the model control group were fed with compound difenoxil and saline, meanwhile, mice in the positive control group were fed with compound difenoxil and Streptococcus thermophilus, and mice in the remaining groups with constipation were fed with compound difenoxil and corresponding concentrations of L. casei. After 3 days of gavage, feces were collected from the intestinal tract of the mice for analysis of flora composition. The results showed that the abundance of Lactobacillus and Alistipes in the intestinal flora of constipated mice was reduced to different degrees, while the abundance of Prevotella and norank_f_Muribaculaceae was increased compared with the normal control group. After 3 days of Lactobacillus casei intervention, the dominant flora were norank_f_Muribaculaceae, Lactobacillus, Prevotella and Cryobacillus. Compared with the model control group, the abundance of Prevotella and norank_f_Muribaculaceae in the intestinal flora of mice in L. casei groups decreased, and the abundance of Alistipes increased; the abundance of Lactobacillus in the L. casei medium-dose group and high-dose group increased. It is suggested that L. casei can improve the intestinal flora and relieve constipation by inhibiting the growth of Prevotella and promoting the growth of Lactobacillus and Alistipes in the intestinal tract.
  • FAN Kuanxiu, LI Qingming, WANG Feng, LI Wenjia, HE Ruiyang, SU Xiaojun
    Food and Fermentation Industries. 2022, 48(6): 71-76. https://doi.org/10.13995/j.cnki.11-1802/ts.029598
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    For exploring the effect of extrusion treatment on the instant solubility and physicochemical properties of yam flour, the flour was processed using a dual-screw extrusion machine and characterized using the scanning electron microscope, X-ray diffraction, Fourier infrared spectrometer, rapid viscosity analysis, etc. Additionally, the instant solubility and main components were analyzed to reveal the internal relationship of instant solubility to the structure changes and main components. The results showed that extrusion treatment remarkably enhanced the yam flour instant solubility; that was, the solubility, water absorption index and water solubility index increased from 10.37%, 251.78% and 9.07% to 58.05%, 278.41% and 57.22% respectively. Mechanically, the extrusion process cracked the starch granules with intact structure into irregular small fragments and adhered together with each other which resulting in the increase of surface area from 0.38 m2/g to 0.65 m2/g and the decrease of average particle size from 65.5 μm to 39.7 μm. On the other hand, the crystal structures of yam flour were damaged by extrusion treatment, and the ordered crystalline regions were turned into disordered and water-binding-prone amorphous regions, these resulted in the reduction of relative crystallinity, gelatinization viscosity and enthalpy. Besides, the contents of starch and amylopectin were decreased, and the contents of dextrin and reducing sugar were increased.
  • HU Ling, YU Mengling, ZHANG Sanshan, ZHANG Jun, LEI Ji
    Food and Fermentation Industries. 2022, 48(6): 77-83. https://doi.org/10.13995/j.cnki.11-1802/ts.026930
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    Fresh noodles are easy to deteriorate and mildew due to their high-water content, and therefore it can be stored only for 5-7 d at 4 ℃. Browning also occurs during storage. To extend the shelf life of fresh noodles, this study try to explore the effects of water activity reducers, preservatives, and acidity regulators on the moisture distribution, water activity, microorganisms, color, sensory score, and shelf life of fresh noodles. The results showed that based on the conventional formula of fresh noodles, 3.0 g sorbitol, 0.1 g capryl monoglyceride, 0.2 g citric acid were added to 100 g microwave-treated wheat flour, and the well-formed noodles were sprayed with 3 mL of 75% edible alcohol, the sealed package of noodles could be safely stored for 56 d at 4 ℃. The combination usage of water activity reducers, preservatives, acidity regulators and spraying alcohol can effectively prolong the shelf life of fresh noodles and solve the browning problem.
  • ZHANG Zixi, MIAO Yanlong, XIAO Yitong, WANG Kai, ZHAO Lei, HU Zhuoyan
    Food and Fermentation Industries. 2022, 48(6): 84-89. https://doi.org/10.13995/j.cnki.11-1802/ts.027452
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    The aim of this study was to evaluate the effects of different concentration techniques on the color and volatile components of longan concentrated juice obtained from atmospheric heating (AH), vacuum concentration (VC), vacuum concentration-baking (VC-B), and dried longan extracts-vacuum concentration (DL). Spectrophotometry and colorimeter were used to analyze the color changes of four kinds of concentrated longan juices, and electronic nose and headspace solid-phase microextraction-gas chromatography-mass spectrometry were used to investigate the volatile components. Results indicated that the color of longan concentrated juice was altered by different concentration processing methods, especially VC-B concentrated juice showed the decrease of L* value and the increase of a* and b* value, which resulting in a reddish-brown and decreased transparency in appearance. The relative content of terpenes was high in the main volatile components of longan concentrated juice. Twenty-four kinds of volatile components were detected in fresh longan juice. However, volatile components of longan concentrated juice obtained by VC-B were increased to 29, and the baking flavor aroma composition of 2-methylpyrazine with 6.54% relativity content and dried longan aroma compound of α-caryophyllene with 0.64% relativity content were detected in this longan concentrated juice. There are unique colors and more volatile components in longan concentrated juice obtained by the vacuum concentration-baking process.
  • LI Qiongshuai, TANG Shanhu, LI Sining, MO Ran, LI Jinjin
    Food and Fermentation Industries. 2022, 48(6): 90-97. https://doi.org/10.13995/j.cnki.11-1802/ts.027602
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    The objective of this study was to explore the effects of pomegranate peel extract (PPE) on the quality and oxidation of yak minced meat products. Minced yak meat treatments were added with 0, 0.01, 0.05, 0.10, or 0.20% (quality fraction) of PPE, and 0.01% (quality fraction) butylated hydroxy toluene (BHT) was served as control. The color differential values, pH, water retention, total bacterial count, carbonyl and total sulfhydryl content, thiobarbituric acid reactive substances (TBARS) value characterization and rheological properties were evaluated. The results showed that PPE could reduce b* value(P<0.05), and which had a certain negative effect on a* value and L* value. PPE could reduce the pH of minced meat and significantly improved water retention, inhibited microbial growth(P<0.05)and improved rheological properties. During storage, protein and fat oxidation occurred continuously in yak meat, and the antioxidant effects of 0.01% and 0.05% were close to those of BHT. Compared with the blank control group, 0.10% and 0.20% PPE significantly delayed the loss of total sulfhydryl group and increased the TBARS value(P<0.05), but the PPE(0.20%) with high mass fraction promoted the formation of carbonyl compounds. In summary, PPE had a good antioxidant capability and the addition amount of 0.20% had the best effect. PPE could significantly improve the physico-chemical properties and rheological properties of yak minced meat products during storage and has a good application prospect in the exploitation of natural preservatives or antioxidants.
  • GAO Jia, TANG Yueming, LUO Fangyao, TIAN Yuxiao, LUO Jinghong
    Food and Fermentation Industries. 2022, 48(6): 98-102. https://doi.org/10.13995/j.cnki.11-1802/ts.027576
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    Temperature is an important factor that affected the shelf-life quality of fresh-cut vegetables. Used yam as the research object, the dynamic changes of O2 and CO2 content, surface browning index, pulp hardness, over visual quality, total bacterial count, polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) in packaged fresh-cut yams under five common shelf-life temperatures of 1, 4, 8, 12 and 20 ℃ during 14 d storage were tested. The results showed that shelf-life temperature significantly affected the dynamic change trend of each measurement index during the storage period. And 1 ℃ was more conducive to maintain the shelf-life quality of fresh-cut yams than other treatments, and its appearance color remained well after 14 d storage. However, the appearance of fresh-cut yams deteriorated rapidly at 20 ℃ and lost its edible value on the 7th day. The total bacterial count of all samples which had no exogenous antibacterial treatment was higher and increased rapidly with the extension of storage time. The activity of PPO and POD were more than 10 times of PAL in fresh-cut yams during shelf-life storage. This research clarifies the quality deterioration law of fresh-cut yams under different shelf-life temperatures and provides information for making reasonable shelf-life sales plan in production.
  • GAO Wenjing, LEI Qiao, WU Hao, CAO Qinglong, WANG Zi
    Food and Fermentation Industries. 2022, 48(6): 103-109. https://doi.org/10.13995/j.cnki.11-1802/ts.027674
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    The 30 W low-temperature plasma was used to scan the surface of the whey protein isolate-sodium caseinate-glycerol composite protein films for 0, 5, 10, 15 and 20 min. The effects of protein secondary structure, microscopic morphology, surface water-oil affinity, mechanical properties, barrier properties, thermal stability and sterilization ability of films were studied. The results showed that after plasma treatment for 5-10 min, the structure, the degree of α-helix and β-sheet in the secondary structure of the protein increased about 15%. In addition, the surface of the film became denser, rougher and showed less porous, and the glass transition temperature (Tg) rose to 150.60 ℃, decomposition temperature (Td) rose to 197.08 ℃. In terms of packaging performance, the tensile strength increased by 24.10%, the elongation at break decreased by 27.13% respectively. At the same time, the barrier properties of the film increased, and the oxygen transmission rate was reduced to 1.20 cm3/(m2·d), the water vapor transmission coefficient was reduced to 5.555×10-12 g·cm/(cm2·s·Pa), and the surface hydrophobicity and oleophobicity were significantly reduced due to their apparent changes (P<0.05). Besides, the light transmittance was reduced by 20%, and the water solubility was also slightly reduced. Good potential in the application of film sterilization treatment was showed that the total number of film colonies was reduced by about 93.93%. Therefore, plasma treatment with a lower energy rate could effectively improve the packaging characteristics of the film and the stability of the protein, enhance its surface roughness to facilitate the composite with other materials.
  • LI Qingfeng, WANG Shiqi, ZHAO He, LI Hong, ZHU Youmin
    Food and Fermentation Industries. 2022, 48(6): 110-116. https://doi.org/10.13995/j.cnki.11-1802/ts.027762
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    This study aimed to investigate the difference in amino acid content and composition of different varieties of Antheraea pernyi and evaluate the nutritional value of tussah pupa comprehensively. The pupae of five tussah varieties in Liaoning province were studied, and the contents of nutrients, fatty acids and amino acids were detected. The nutritional value and flavor of tussah pupae were assessed by the amino acid score (AAS), chemical score (CS), nutritional index (NI), essential amino acid index (EAAI) and biological value (BV). The results showed that all tussah pupae were rich in nutrients and contain 18 amino acids, so they were ideal animal protein resources. The contents of essential amino acids and total amino acids of Liaocan582 and Kangda were higher. The mass fraction of crude fat of Kangda was the lowest, but the mass fraction of crude protein and the relative content of unsaturated fatty acid of Kangda was the highest. At the same time, Kangda riched in delicious amino acids, and the proportion of amino acids of Kangda was more suitable for human needs. Liaocan582 showed the highest EAAI, NI and BV values, having high relative nutritional value and protein digestibility. The comprehensive evaluation of nutritional components and flavor showed that Kangda and Liaocan582 had a higher nutritional value and better flavor. The results provide a theoretical basis for breeding and food development of tussah pupae with high nutritional value.
  • SUN Tongsheng, WANG Jinzhi, WU Huidong, LING Feng
    Food and Fermentation Industries. 2022, 48(6): 117-124. https://doi.org/10.13995/j.cnki.11-1802/ts.029234
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    To improve the quality of Lentinus edodes after hot air drying, fresh Lentinus edodes were used as raw materials for the treatment of variable temperature drying and intermittent drying. Experimental tests were carried out in five aspects: temperature, color, moisture content, energy consumption and rehydration ability, and compared with the constant temperature drying. The results showed that the temperature distribution of Lentinus edodes dried by variable temperature drying was more uniform. Not only the temperature difference of a single Lentinus edodes was small, but also the temperature rise of different Lentinus edodes was the same. The temperature field uniformity of intermittent drying was the second. After variable temperature drying and intermittent drying, the color parameters of Lentinus edodes changed little. The brightness value, red-green value and yellow-blue value decreased only slightly, and the maximum color difference values were 7.42 and 7.87 respectively. However, the maximum color difference value of Lentinus edodes after constant temperature drying was as high as 22.38. After constant temperature drying, the water content of Lentinus edodes decreased rapidly, and the rehydration rate was the lowest, at 92.59%. The rehydration rate of variable temperature drying was the highest, up to 143.65%. The energy consumption per unit time of intermittent drying was 0.056 kW·h, which was lower than 0.105 kW·h of constant temperature drying and 0.083 kW·h of variable temperature drying. Therefore, variable temperature drying has a significant effect on improving the quality of Lentinus edodes, while intermittent drying has an obvious energy-saving effect. The results can provide a reference for the optimization of the hot air-drying process under different drying requirements.
  • LIU Yuan, ZHU Xuran, WANG Jian, LIU Yang, WAN Yuping, WU Xiaosheng, LI Na, WANG Jiying, LIU Jia, MIAO Yutian
    Food and Fermentation Industries. 2022, 48(6): 125-133. https://doi.org/10.13995/j.cnki.11-1802/ts.026502
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    The purpose of the research is to investigate the effects of electrolyzed functional water (EFW) treatment on the removal of organophosphorus (phorate and chlorpyrifos), pyrethroid (lambda-cyhalothrin and cyfluthrin) and fungicide (procymidone and chlorothalonil) from the surface of fresh-cut cabbage, broccoli and color pepper. The simulating pollution was carried out by soaking three fresh-cut vegetables in a mixture of pesticides. And the vegetable samples were washed by different treatments, and after which the pesticide removal rates were determined by QuEChERS combined with GC-MS. The effects of washing solution, the ratio of sample to washing solution, washing temperature, washing method and washing time on pesticide removal rates were studied. The results showed that acidic electrolyzed water (AcEW) could efficiently remove pesticide from cabbage and broccoli comparing with other traditional washing solutions, and alkaline electrolyzed water (AlEW) had a better removal effect on pesticides in color pepper. Besides, AcEW showed higher degradation efficiency for pyrethroid and organophosphorus, whereas AlEW was more sensitive to fungicides. The optimal ratio of sample to washing solution for fresh-cut cabbage, broccoli and color pepper was 10∶400, 10∶1 600 and 10∶800, respectively. The pesticide removal rates at 25 ℃ were not significantly different from those at 45 ℃ (P>0.05), but they were significantly higher than those at 4 ℃ (P<0.05). In general, the optimal washing method for fresh-cut cabbage was intermittent oscillation, and the corresponding method for broccoli and color pepper was continuous oscillation. With the treatment of these conditions for 20 minutes, the removal rates of 6 pesticides in fresh-cut cabbage, broccoli and color pepper were 72.28%-91.04%, 72.28%-90.11% and 72.24%-88.12%, respectively. The change patterns of pesticide reduction obtained in this study provide theoretical and technical support for the application of EFW in pesticide residues removal from postharvest leafy, flower and fruit vegetables.
  • LIU Yuxuan, LI Binbin, HUANG Xiaohong, LAN Qinjie, DENG Lin, PEI Huijie, WANG Yilun, LIU Shuliang, YANG Yong
    Food and Fermentation Industries. 2022, 48(6): 134-140. https://doi.org/10.13995/j.cnki.11-1802/ts.028344
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    In order to explore whether exogenous addition of diamine oxidase has influence on the sensory quality of Sichuan-style sausage, and its ability to degrade biogenic amines, Sichuan-style sausage with exogenous addition of diamine oxidase was used as the experimental materials, and the sensory quality and biogenic amine contents were measured. Results showed that there was no significant difference between the control group and experimental group based on sensory assessment, electronic nose and tongue, texture profile as well as color analysis, indicating that the addition of diamine oxidase had no significant effect on the sensory quality of Sichuan-style sausage. In addition, the total biogenic amine content of Sichuan-style sausage decreased from 316.50 to 160.91 mg/kg after adding diamine oxidase, in which histamine, tyramine, cadaverine and putrescine decreased by 54.47%, 52.68%, 43.69%, 6.29%, respectively. Exogenous addition of diamine oxidase had no obvious effect on the sensory quality of Sichuan-style sausage, but it could significantly reduce the contents of total biogenic amines and four main biogenic amines, thereby improving the safety of Sichuan-style sausage.
  • WANG Xiaochao, CHENG Ni, DUAN Qianqian, ZHAO Cheng, XU Yuanyuan, CHEN Sinan, CAO Wei
    Food and Fermentation Industries. 2022, 48(6): 141-146. https://doi.org/10.13995/j.cnki.11-1802/ts.028587
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    Bee pollen is rich in phenolic compounds, which owns the capabilities of antioxidant, antibacterial, anti-inflammatory. Therefore, the absorption and utilization of phenolic compounds in bee pollen are closely related to human health. In this study, the total phenolic, total flavonoids content, as well as antioxidant activity of three kinds of bee pollen (rape bee pollen, Schisandra chinensis bee pollen and Camellia japonica pollen) were measured in different digestion stages and fermentation times by gastrointestinal digestion and colonic fermentation in vitro, then, the bioaccessibility of the phenolic compounds was further analyzed. The results showed that the total phenolic content, total flavonoids content and antioxidant activities of these three kinds of bee pollen decreased after digestion in vitro, and their bioavailability also diminished. However, in colonic fermentation, with the prolongation of fermentation time, the total phenolic content, total flavonoids content and antioxidant activities of the three bee pollens significantly increased (P<0.05). The total phenolic content of rape bee pollen, Schisandra chinensis bee pollen and Camellia Japonica pollen reached 5.99, 7.17 and 6.38 mg/g after 24 h of colonic fermentation, and the bioaccessibility of phenolic compounds reached 126.96%, 139.74% and 120.54%, respectively. These findings suggested that colonic fermentation could promote the release of phenolic compounds and improve the bioavailability of phenolic compound in bee pollen, which is the key point in the role played by bee pollen polyphenols in vivo. This study will provide a theoretical basis for exploiting new bee pollen products by probiotics fermentation.
  • ZHAO Shiguang, XIE Dongbao, CHU Xinying, ZHANG Yu, HU Zimin, GE Litao, QIAN Senhe, WEI Ming
    Food and Fermentation Industries. 2022, 48(6): 147-153. https://doi.org/10.13995/j.cnki.11-1802/ts.029560
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    Using tea seeds as the raw material, the tea seed polypeptides (TSP) were prepared from the two-stage mixed fermentation with Ganoderma lucidum UIM-281 and Bacillus amyloliquefaciens PI142. Meanwhile, TSP antioxidant activities were studied. Plackett-Burman design and response surface experiment were used to optimize the fermentation conditions. The broth was separated by ultrafiltration. In vitro antioxidant activities of TSP were determined. The results showed that the optimum mixed fermentation conditions were as follows: tea seed 75.23 g/L; glucose 4.0 g/L; UIM-281 inoculum size 1.0%; initial pH 9.20; 24 h of the first stage fermentation; PI142 inoculum size 0.5%; 24 h of the second stage fermentation, and the whole process was at constant temperature of 40 ℃ and shaking speed of 120 r/min. Under these conditions, the TSP yield was up to 8.97 mg/mL. The fraction Ts with molecular weight of 1-5 kDa was obtained by ultrafiltration. The IC50 values of Ts toward ABTS, DPPH and ·OH free radical scavenging rates were 0.358, 0.164 and 3.537 mg/mL, respectively. TSP exhibited notably higher antioxidant activity than that of non-fermented tea seeds, which could provide theoretical guidance for the high-value utilization of tea seeds and tea meal resource.
  • HU Song, WANG Penghui, LIU Binjie, SU Jingqian, TAN Qingqing, YANG Minhe, WANG Guohong
    Food and Fermentation Industries. 2022, 48(6): 154-159. https://doi.org/10.13995/j.cnki.11-1802/ts.029772
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    To compare the physicochemical properties of fermentation broth of black tea, green tea and Ginkgo biloba leaves fermented by kombucha, explore its antibacterial effects on pathogens and multi-drug resistant bacteria, and compare the effects of fermentation time on the antibacterial effects of ginkgo kombucha, the changes of fermentation broth appearance, biofilm, pH value, total acidity and reducing sugar content were measured and compared after fermented for 7 days. The inhibitory effects of different kombucha broth on pathogens were determined. Results showed that there were significant differences in the appearance and biochemical properties among these three kinds of kombucha after 7 days of fermentation. The pH value of fermentation broth decreased to around 3, and the total acidity was more than 4 g/L. Compared with black tea kombucha and ginkgo kombucha, the growth of bacteria in green tea kombucha broth was the best, with thicker biofilm formation. Black tea kombucha had inhibitory effects on sensitive strains of Propionibacterium acnes, Staphylococcus aureus and Pseudomonas aeruginosa to some extent. However, there was no activity against Bacillus subtilis and three multi-resistant bacteria strains such as Acinetobacter baumannii, Escherichia coli T5 and Escherichia coli P1-D6. Fermentation time had significant effect on the antibacterial activities of ginkgo kombucha. Fermentation broth on the 17th day had the strongest inhibitory effects against P. aeruginosa and E. coli T5. The ranking of antibacterial activities was green tea kombucha>ginkgo kombucha>black tea kombucha. Fermentation broths of green tea kombucha and ginkgo kombucha had the activity of inhibiting Streptococcus pneumoniae, multi-resistant isolates such as E. coli and Acinetobacter baumannii. These results can provide experimental basis for the development of new food preservatives and antibacterial drugs.
  • LI Yufang, SHI Yanan, LI Xiang, SHI Chongying, HUANG Aixiang
    Food and Fermentation Industries. 2022, 48(6): 160-167. https://doi.org/10.13995/j.cnki.11-1802/ts.028267
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    To explore the influence of fermentation time on the volatile flavor compounds of Dahe black pig ham during traditional processing (salted for 21 d, dehydrated for 60 d, fermented for 90-630 d), solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) technology combined with partial least squares-discriminant analysis (PLS-DA) and relative odor activity value (ROAV) were used to analyze the volatile flavor compounds of Dahe black pig ham fermented for 90, 150, 210, 270, 450, 630 d. The results showed that a total of 135 volatile flavor components were identified during these six fermentation periods, including aldehydes, alcohols, acids, hydrocarbons, ketones, esters, and other compounds, among which the most abundant flavor families were aldehydes. The volatile flavor components of four different fermentation periods (210, 270, 450, 630 d) were similar. It indicated that the principal flavor compounds had formed at 210th days during fermentation. A total of 17 key flavor compounds were identified during the fermentation, including hexanal, heptanal, octyl, aldehyde, nonanal, 2-octenal, and other substances. Fermentation time had a certain impact on the formation of volatile flavor compounds in ham, and the processing time of Dahe black pig ham could be set as 10-12 months. This research provides a theoretical basis for the formation mechanism of volatile flavor compounds in Dahe black pig ham.
  • QIAN Zhenwen, WU Zongwen, WU Dianhui, LU Zhendong, XIE Guangfa, HU Zhiming, QIU Zheling, MAO Qingzhong
    Food and Fermentation Industries. 2022, 48(6): 168-173. https://doi.org/10.13995/j.cnki.11-1802/ts.028232
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    Insufficient acidity is a common problem in the production of low-alcohol Huangjiu. In order to produce high-acidity Huangjiu for acidity adjustment in low-alcohol Huangjiu production, bioaugmentation fermentation with lactic acid bacteria in Huangjiu brewing was studied. Through single factor and uniform design experiment, the optimal parameters for producing high-acidity Huangjiu were as follows: the addition time of lactic acid bacteria was the same as the inoculation time of yeast, lactic acid bacteria inoculum size of 5.3%, wheat Qu dosage of 17%, ratio of rice to water of 1∶2.5, and 24 ℃. Under these conditions, the content of total acidity and alcohol of high-acidity Huangjiu reached 12.5% vol and 17.5 g/L, respectively. Compared with the control group, the levels of total acidity and lactic acid increased by 326.8% and 397.2%, respectively. While the content of biogenic amines decreased by 34.5% and the content of higher alcohols decreased by 35.3%. Therefore, the product quality and safety of low-alcohol Huangjiu can be improved by acidity adjustment with high-acidity Huangjiu, which was fermented through bioaugmentation with lactic acid bacteria.
  • ZHAO Hongzhou, DU Muying, ZALAN Zsolt, HEGYI Ferenc, WU Liangru, ZHANG Tongfang, ZHENG Jiong
    Food and Fermentation Industries. 2022, 48(6): 174-180. https://doi.org/10.13995/j.cnki.11-1802/ts.029771
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    To explore the effect of bamboo shoot (Chimonobambusa quadrangularis) dietary fiber (BSDF) as prebiotics on the growth of lactic acid bacteria, the anti-digestibility of BSDF in simulated gastric and intestinal fluid, and the impact of BSDF on pH value, total acid content and growth curve of Lactobacillus fermentum (L1) and Lactobacillus plantaum (L2) during growth were investigated with inulin as control. The results showed that the total dietary fiber, insoluble dietary fiber, and soluble dietary fiber content in BSDF was 63.90%, 58.12%, and 6.78%, respectively, and the monosaccharides with relatively higher content were glucose (39.72%), arabinose (12.77%), and xylose (12.69%). BSDF had anti-digestibility in gastric and enteric fluid, and the hydrolysis rate of BSDF after simulated digestion in gastric fluid (pH=3.5) and enteric fluid (pH=7) for 6 h was 15.54% and 31.08%, respectively, lower than that of inulin. For L1, the concentration of bacteria in the medium increased with the increase of BSDF addition. BSDF was more beneficial to L1 growth than inulin. For L2, with the increase of BSDF, the total acid content increased, and the probiotic effect of lactic acid bacteria was also enhanced. This study can provide theoretical reference for BSDF as a prebiotic on lactic acid bacteria.
  • LIU Ting, ZHAO Liangzhong, ZHOU Xiaohu, HE Chuman, ZHANG Yanyan, ZHANG Xuejiao
    Food and Fermentation Industries. 2022, 48(6): 181-187. https://doi.org/10.13995/j.cnki.11-1802/ts.029778
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    To investigate the substrate utilization and changes of soybean isoflavone metabolism and flavor substances during the fermentation of soybean milk, different kinds and concentrations of carbon sources were added to soybean milk. Acidity and sensory score were taken as evaluation indexes. The results showed that with the increase of sucrose, lactose and glucose ratio, the sensory scores of taste, smell and comprehensive acceptance improved significantly (P<0.05), while the sensory scores of texture and color were not affected. The contents of isoflavones glycoside decreased linearly, and fermented soybean milk with 0.03 g/mL gluconate had the highest decreasing rates of isoflavones daidzein and genitin, up to 92.62% and 88.13%, respectively. The aglycone isoflavones of fermented soybean milk with 0.03 g/mL sucrose were transformed firstly. During the fermentation of soybean milk, a total of 72 volatile compounds were detected by GC-MS, including seventeen esters, sixteen acids, fifteen ketone, eleven alcohols, four aldehydes. The substances with unpleasant flavor, such as 1-octene-3-ol and hexanal, could not be detected after fermentation, and new pleasant flavors were formed.
  • LIN Yukuan, WU Shengwen, YOU Yong, LIAO Chang, LIN Pei, DING Zhongtao, WU Xiaoyu
    Food and Fermentation Industries. 2022, 48(6): 188-194. https://doi.org/10.13995/j.cnki.11-1802/ts.027808
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    Differences in microbial diversity and physicochemical indices between Daqu produced by traditional culture technique (TT) and new culture techniques (NT) were analyzed at the Daqu workshop of Site Liquor Co. Ltd. using high-throughput sequencing and physicochemical index assays. The temperature of NT Daqu fermentation products increased slowly in early stage of fermentation, and cooled slowly later. Cellulase and acid protease levels, saccharifying and liquefying power, carbon and nitrogen biomass of NT Daqu were higher than those in TT Daqu at the end of fermentation. Based on high-throughput sequencing, the relative abundance of Lactobacillus in late stage of NT Daqu fermentation (20 d) was much higher than that in TT Daqu. Considerable differences were observed in dominant fungal genera between NT and TT Daqu. The relative abundances of Rhizopus, Hyphopichia, and Candida in late stage of NT Daqu fermentation (20 d) were higher than those of TT Daqu. Redundancy analysis (RDA) between the Daqu microbial community and its physicochemical indices further indicated that its physicochemical indices were closely related to the structure and abundance of its microbial genera. Comparison of these two techniques revealed that the new technique was superior to traditional technique in terms of Daqu culture temperature, physicochemical indices, microbial diversity, and dominant flora, which could be a theoretical foundation for technical improvements in the cultivation process, enhancing the quality of Daqu.
  • DING Feihong, GENG Yunlong, FAN Shide, PENG Shuangshuang, ZOU Xi, ZHAO Yifeng, CHEN Teng, GAO Zhaojian
    Food and Fermentation Industries. 2022, 48(6): 195-203. https://doi.org/10.13995/j.cnki.11-1802/ts.028471
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    To isolate antagonistic Bacillus strains from traditional sausage, and to purify antibacterial substances produced by the strains and analyze their antibacterial characteristics, Bacillus strains producing antibacterial substances were screened by plate confrontation method. The antibacterial substances were separated by organic solvent extraction, Sephadex LH-20 column chromatography, semi preparation reverse high performance liquid chromatography (RP-HPLC), and identified by Fourier transform infrared spectroscopy (FT-IR) and thin layer chromatography (TLC)-bioautography. A strain producing broad-spectrum antibacterial substance was isolated and identified as Bacillus pumilus (named XZK-18). Strain XZK-18 began to synthesize antibacterial substances after 8 h of fermentation, and the yield reached the maximum at 24 h. The antimicrobial active component was identified as lipopeptide by TLC, and it was mainly composed of surfactant and a small amount of iturin. TLC-bioautography showed that the surfactant had significant antibacterial activity against Staphylococcus aureus and Escherichia coli. FT-IR further confirmed that the antibacterial active component was lipopeptide. The results of scanning electron microscope demonstrated that the lipopeptide killed E. coli by destroying the integrity of cell envelope, perforating the cell wall and increasing the permeability of cell membrane. The lipopeptide had broad-spectrum antibacterial activity, and had significant antibacterial activity against Gram-positive bacteria such as Bacillus cereus, Bacillus subtilis, S. aureus, Micrococcus gaminerae and Listeria monocytogenes; it also had certain antibacterial effect on Gram-negative bacteria such as Pseudomonas aeruginosa, E. coli, Salmonella typhi, Vibrio parahaemolyticus and Shigella flexneri. It had no antimicrobial activity to the tested fungi. The lipopeptide was stable below 110 ℃ for 30 min, and its antibacterial activity remained stable at pH 3-11 for 10 d. These characteristics indicate that the lipopeptide synthesized by strain XZK-18 has potential as a natural food preservative in food production and processing.
  • GONG Lianjin, BO Jiahui, ZAHNG Tiantian, SUN Hongyu, CHEN Yiqin, PEI Ruoyun, XIAO Lizheng
    Food and Fermentation Industries. 2022, 48(6): 204-209. https://doi.org/10.13995/j.cnki.11-1802/ts.027870
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    In order to explore the dynamic changes of β-glucosidase(β-GC), alcohol dehydrogenase(ADH), lipoxygenase(LOX) and main aroma components in the processing of withering, rolling, and fermentation of Huangjincha black tea, this study used Huangjincha with one bud and two leaves as raw materials and processed according to black tea technology, and then the activities of β-GC, ADH, LOX and the changeable regularities of aroma components during the processing were measured. The results showed that the activities of β-GC, ADH, and LOX all reached the highest in the withering processing, and then continued to decline in rolling and fermentation. Two hundreds and sixty-three kinds of aroma components were detected, including 53 alcohols, 53 alkenes, 70 alkanes, 36 esters, 14 ketones, 17 aldehydes and 20 other compounds, among which the relative contents of alcohols and esters were much higher than those of other aroma types. In addition, aroma components with relatively high content were pheneylethanol, geraniol, trans-nerolidol, linalool, methyl salicylate, (4E)-4E-hexyl hexanoate, dihydroactinidiolide, benzaldehyde, (E)-oct-2-enal, heptadienal, β-cyclocitral, α-farnesene, (-)-α-cubebene, cis-jasmone, and β-ionone. The fragrance of Huangjincha black tea belonged to linalool and its oxide-dominated fragrance. The results enriched the basic chemical theory of the flavor quality of Huangjincha black tea, and provided a scientific evidence for the production of high-quality Huangjincha black tea.
  • WANG Jieping, LIU Rui, DING Shiyong, LU Qun
    Food and Fermentation Industries. 2022, 48(6): 210-217. https://doi.org/10.13995/j.cnki.11-1802/ts.028169
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    Lyophilized powder of queen bee larvae was used as raw material, the antioxidant peptides were prepared by mixed fermentation, and their antioxidant activity and structure were studied. Taking peptide yield and protease activity of the fermentation broth as evaluation indexes, the optimum fermentation process was determined by single factor and response surface experiments. The crude peptides were separated by ultrafiltration. The amino acid composition of crude peptides and the in vitro antioxidant activity of the components of crude peptides were studied. In addition, the amino acid sequence of ultrafiltration component with the best antioxidant activity was identified by LC-MS/MS. The results showed that the optimum fermentation conditions were as follows: the ratio of Bacillus subtilis to Aspergillus niger, 2.4∶1; inoculation amount of 6.5%; fermentation time of 57.5 h. Under these conditions, the peptide yield was 15.49%, and the protease activity was 133.55 U/mL. The content of antioxidant amino acids and hydrophobic amino acids in the crude peptides of queen bee larvae was 58.11% and 47.2%. Five components F1-F5 were obtained by ultrafiltration. Component F1 with molecular weight less than 1 kDa had the best antioxidant activity. Its IC50 values of DPPH free radical and ABTS cation ion scavenging rate was (1.06±0.01) and (0.165±0.004) mg/mL, respectively, and iron reducing power OD700=0.389 (2 mg/mL). The F1 was separated and identified by LC-MS/MS and compared with Mascot peptide database. Nine possible peptides with molecular weight ranging from 600 to 1 100 Da were obtained. The results provide experimental basis for the development of antioxidant peptide products of queen bee larvae.
  • YIN Linlin, ZHANG Xue, LU Shifeng, WANG Xuanyan, MAO Liping
    Food and Fermentation Industries. 2022, 48(6): 218-224. https://doi.org/10.13995/j.cnki.11-1802/ts.026775
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    The aim of this research was to explore the preservation effect of short-wave ultra-violet (UV-C) irradiation combined with 15 g/L chitosan coating (CH-C) on fresh-cut sugarcane. The untreated group (CK) was used as controls, the indexes of individually treated groups and synergistically treated (UV-C+CH-C) fresh-cut sugarcane were analyzed including pH value, soluble solids(SSC), browning degree, total bacteria count (TBC), total mold and yeast count (MYC), total phenol, malondialdehyde (MDA), polyphenol oxidase (PPO)and peroxidase (POD) during cold storage at 4 ℃. The results showed that the UV-C, CH-C, and UV-C+CH-C groups could effectively maintain the quality of fresh-cut sugarcane compared with the CK group for 30 days. Among which, pH value and SSC stability improved obviously and speed of browning reduced by 33.6%, 65.6% and 70% respectively. In addition, TBC increased 19.5% and 3.5% in UV-C and CH-C, but reduced 36.3% in UV-C+CH-C groups. Moreover, MYC, total phenol content, MDA content, PPO with POD activity were reduced in different degree respectively. UV-C+CH-C could extend the cold storage shelf life of vacuum-packed fresh-cut sugarcane from 6 d to 30 d using TBC and MYC combined with pH and sensory changes as indicators. UV-C+CH-C treatment has a better preservation effect on fresh-cut sugarcane. This study could provide a reference for the effective and economic preservation of fresh-cut fruits and vegetables.
  • BU Junzhi, XU Xun, TANG Zhenxing, BAI Feng
    Food and Fermentation Industries. 2022, 48(6): 225-232. https://doi.org/10.13995/j.cnki.11-1802/ts.026638
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    This study explored the influence of sous vide-processing on the quality of carrots, the nutritional, structural and sensory properties of carrot processed with sous-vide and compared with other traditional processing treatments such as boiled carrot and fried carrot. The results indicated that in contrast to the conventionally processed carrots, a slight reduction in the content of protein, total carbohydrate, total phenol, carotene was observed for the sous-vide carrot. Sensory evaluation results showed that sous-vide samples had a better appearance, aroma and taste than traditionally processed ones. The result of volatile flavor substances analyses revealed that terpenes which were characteristic flavor substances of carrots, were better preserved in sous-vide than conventionally processed carrots. The comprehensive analyses show that the sous-vide process can be used for the treatment vegetables in order to get better quality products.
  • YANG Huiru, GUO Jun, Gusilengtu, WANG Yuenan, ZHANG Chunhua, SUN Haizhou
    Food and Fermentation Industries. 2022, 48(6): 233-240. https://doi.org/10.13995/j.cnki.11-1802/ts.027899
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    A total of 55 representative milk samples of Holstein cow(cow), Mongolian horse(horse), Bactrian camel, goat, buffalo and yak were collected for the determination of 18 amino acids(AA) by traditional descriptive statistics, nutritional evaluation and cluster analysis. The results showed that the amino acids absolute content (mg/g) were significantly different (P<0.01). Total amino acid(TAA)of the six animal milk were 1.7-5.4 times higher than that of mix human milk released from Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO). And in which, TAA of yak and buffalo were the highest, followed by the goat, camel, cow and horse milk. Principal component analysis(PCA) on percentage data of AA (AA/TAA, %) showed that clustering of six livestock milk samples in 3D space conformed to taxonomy class of the animal’s species, four Bovidae animal milk samples clustered in the same region, while the cluster of horse and camel milk samples were far in another two regions. Human milk was close to horse milk. Trp, Cys, Asp and Arg in human milk were relatively insufficient in livestock milk. The amount of Trp in Goat was close to human milk. Essential amino acid (EAA) pattern of livestock milk was significantly different from human milk (P<0.01). PCA showed that the milk of six livestock was relatively together, while the human milk was far away from them, which implied that the EAA pattern of human milk was significantly different from that of animals. Goat and horse milk were relatively close to human milk according to their EAA pattern. Moreover, soft independent modeling of class analogies species discrimination model was established based on milk AA% data, and the internal and external verification accuracy rates were 100% and 92.76% respectively. External validation errors appeared within the Bovidae milk samples. Human milk cannot be classified into any one of the six animals which indicating that the amino acids of human milk and animals were different. Chemometrics cluster analysis is more visual and holographic than traditional descriptive statistics and protein amino acids nutritional evaluation. This study can provide innovative and creative strategies, principles and methods for the evaluation of nutrition and authenticity of dairy products.
  • CHENG Yujiao, LI Guijie, OUYANG Zhu, WU Houjiu, LIANG Guolu, WANG Hua
    Food and Fermentation Industries. 2022, 48(6): 241-249. https://doi.org/10.13995/j.cnki.11-1802/ts.028167
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    Clementine is a kind of mandarin hybrid fruit with unique flavor and rich nutritional values. The volatiles were analyzed qualitatively and quantitatively in clementine juice at five different stages during maturity by the combination of headspace solid phase micro extraction (HS-SPME) and gas chromatography-mass spectrometry/pulsed flame detector (GC-MS/PFPD). The flavor variation of clementine juice at different maturity stages was investigated. The results showed that 41 volatile compounds were identified in clementine juice. Terpenes accounted for 91.89%-96.63% of total volatiles, among which, D-limonene and β-myrcene were the highest. Principal component analysis (PCA) showed that the distribution of ripening stage 1 was closely related to terpenes, while the distribution of other ripening stages was closely related to terpenes, alcohols, aldehydes and esters. According to aroma activity value (OAVs), 29 characteristic flavor components (OAV≥1) were screened out, and the number of special flavor components in ripening stage 4 was higher than that in other stages. In addition, the processing promoted the formation of methanethiol and methylene sulfide, and the odor was best at ripening stage 4. The ripening period affects the processing flavor quality of clementine juice. This study provides a theoretical basis for defining the picking time of clementine which has the suitability for processing.
  • LUO Xiaoye, ZHAO Haojing, BAN Shidong, WANG Xiaodan
    Food and Fermentation Industries. 2022, 48(6): 250-256. https://doi.org/10.13995/j.cnki.11-1802/ts.027229
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    The conventional cultivation formula of Lentinus edodes, including 78% sawdust, 18% bran, 1% gypsum and 1% sugar, was used as control, and the proportion of wheat bran was taken place by rapeseed cake in the cultivation substrate (25%, 15%, 10%, 5%, 2%) in order to investigate the differences of volatile flavor components in Lentinus edodes. Headspace solid-phase microextraction-gas chromatography-mass was used to determine the volatile flavor components. The results showed that 55 kinds of volatile flavor compounds were determined and analyzed in six kinds of Lentinus edodes samples with different multi-potency compound formulas, which including alcohols, aldehydes, ketones, esters, ethers, alkanes and olefins. When the replacement ratio of rapeseed cake was 10%, the varieties and total contents of volatile flavor components were increased, which were 50 and 97.5%, respectively. The principal component analysis showed that there were some differences in the flavor quality of Lentinus edodes cultivated with different multi-potency compound formulas, and formula 3 (F=15.841) had the best flavor quality. By cluster analysis, six different formulas were divided into three groups according to the content of flavor components. The rapeseed cake of the first cluster (formula 1) and the second cluster (formula 2) was added more than ordinary level, which made the volatile substances of alcohols, aldehydes, esters and alkanes lower than those of other formulations. This study analyzed the volatile flavor components of Lentinus edodes cultivated with different multi-potency compound formulations and provided a theoretical basis for improving the flavor quality of Lentinus edodes in conventional production.
  • FENG Jing, HUANGFU Jie, DONG Jianhui, HAN Ying, LIU Rong, WANG Cheng, SONG Tao, WANG Lihua, ZHANG Jiaojiao, HAN Xinglin, WANG Deliang
    Food and Fermentation Industries. 2022, 48(6): 257-262. https://doi.org/10.13995/j.cnki.11-1802/ts.027554
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    Lujiu is a type of traditional Chinese liquor made from distilled liquor or fermented wine, and is blended, flavored or reproduced with additives, edible food materials, and/or medical herbs. The present study focused on consumers’ sensory perception and acceptance of Lujiu. Explicit self-report and implicit method using facial expression analysis were incorporated in this study. Consumers were invited to evaluate three Lujiu samples with different alcohol content and sugar content. They were video recorded during each evaluation session and their facial expressions were analyzed using FaceReader. The evaluation process was divided into four sessions of looking, smelling, tasting and after-tasting. ANOVA and MFA (Multiple Factor Analysis) were used in data analysis. The result showed that consumers gave high positive emotional responses such as happiness and surprise when tasting samples with high sugar content (95 g/L), which was consistent with the results of the preference questionnaire, indicating that products with higher sugar content were more popular. MFA results showed that sweetness, mouthfeel and fruitiness were closely related to acceptance, which was in accordance with the higher scores of the samples with high sugar content in sweetness, fruity aroma, soft and harmonious taste. The incorporation of facial expression and sensory evaluation was proven to be an effective and comprehensive method in measuring consumers’ affective response in Lujiu evaluation. This study established an effective method to evaluate the consumer acceptance of Lujiu through facial expression analysis technology, and provided a basis for further research.
  • GE Liang, LI Na, YANG Minghan, TIAN Shuge
    Food and Fermentation Industries. 2022, 48(6): 263-269. https://doi.org/10.13995/j.cnki.11-1802/ts.030123
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    A high-performance thin-layer chromatography (HPTLC) method was established for the qualitative and quantitative analysis of malic acid and quinic acid in Hippophae rhamnoides L. fruits, and an ultraviolet method was established to determine the clearance rate of Hippophae rhamnoides L. fruits to DPPH free radicals, ABTS cationic free radicals and superoxide anion radicals(·O-2). Took V(dichloromethane)∶V (ethyl acetate)∶V (formic acid) =5∶6∶2.5 as the developing reagent, the CamagTLC3 scanner was used to determine the content of malic acid and quinic acid in Hippophae rhamnoides L. fruits from 10 different producing areas. The antioxidant ability of Hippophae rhamnoides L. fruits was evaluated by DPPH method, ABTS method, ·O-2 method. The qualitative and quantitative analysis results showed that the Rf value of malic acid was 0.59±0.05, and the Rf value of quinic acid was 0.45±0.05. The methodological investigation results showed that the analytical method of HPTLC was good. Furthermore, the antioxidant results showed that within the test concentration range, the IC50 of S7-S10 Hippophae rhamnoides L. fruits extract DPPH free radicals was 0.11-0.13 mg/mL and the maximum clearance rate was 93.71%-96.27%. The IC50 of ABTS cationic free radicals was 0.11-0.61 mg/mL and the maximum clearance rate was 92.18%-99.39%. And the IC50 of ·O-2 was 1.80-2.90 mg/mL and the maximum clearance rate was 81.92%-100.07%. The clearance rates of DPPH free radicals, ABTS cationic free radicals, and·O-2 were all less than vitamin C. It was showed that the Hippophae rhamnoides L. fruit extract had a good dose-dependent DPPH free radical, ABTS cationic free radicals and ·O-2 antioxidation ability within a certain concentration range. The method established was simple and could be widely promote to the rapid detection of Hippophae rhamnoides L. fruits and provided a certain reference for the quality control of Hippophae rhamnoides L. fruits. This study showed that Hippophae rhamnoides L. fruits was medicinal and food plants with high antioxidant properties.
  • ZHANG Yuan, LIAO Weifang, MIAO Lihong, YANG Tuanyuan, LIU Pulin, YANG Yibin
    Food and Fermentation Industries. 2022, 48(6): 270-275. https://doi.org/10.13995/j.cnki.11-1802/ts.027843
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    Lactobacillus jinshani is a functional strain widely existing in the Baijiu brewing system. In order to rapidly and accurately detect the quantity and the quality of L. jinshani in Baijiu brewing, a quantitative real-time PCR (q-PCR) detection method was established by selecting and optimizing specific amplification primers of L. jinshani. In this study, a pair of specific primers with the size of 266 bp were designed according to the whole genome sequence of Baijiu-making strain L. jinshani. The tests of specificity, sensitivity and repeatability were carried out. Only L. jinshani could be detected among 13 common Baijiu brewing microorganisms, and the minimum detection amount was 3.08×103 copies/μL, with the coefficient of variation of reproducible results≤3.06%. Furthermore, the samples of Daqu and different rounds of fermented grains were tested, and it was found that no L. jinshani was detected in Daqu and the first round of fermented grains, the content of L. jinshani in the second round of fermented grains was (8.9±0.11) lgcopies/g, the content of L. jinshani in the fourth round of fermented grains was (9.38±0.11) lgcopies/g, and the content of L. jinshani in the fifth round of fermented grains reached (9.43±0.11) lgcopies/g, which was consistent with the results of high-throughput sequencing. The results showed that the fluorescence quantitative PCR method can be used for rapid identification and quantitative detection of L. jinshani in Baijiu brewing system.
  • ZENG Dingling, FANG Changfa, GU Yaping, PAN Jianlei, LIN Huichun, ZHANG Xiaoqiang, ZHANG Xiaohong, WANG Yuxin
    Food and Fermentation Industries. 2022, 48(6): 276-280. https://doi.org/10.13995/j.cnki.11-1802/ts.026945
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    A method for the determination of avermectin residues in fruits and vegetables was established in this study. Four matrices were treated with modified QuEChERS and liquid chromatography-tandem mass spectrometry (LC-MS-MS) with atmospheric pressure chemical ionization (APCI). The stability, matrix effect and method accuracy of the method was ed. The stability in 5 hours of the standard solution was below 5% tested every 1 hour. The results showed that correlation coefficients of standard curves in four matrices were greater than 0.99 in the linear range of 0.012 5-0.40 mg/L; the matrix effect on the APCI mode was between 0.97 and 1.05. The spiked recovery results of avermectin in four matrices were 93.3% and 100.8% with the RSD of 1.8% to 5.7% (when in the adding amount of 0.01, 0.1 and 0.2 mg/kg). The proposed method was more suitable for the quantitative analysis of avermectin in agricultural products for its stability, convenience, accuracy and less matrix effect.
  • LIU Jianhua, ZENG Qianhua, XU Xia, DING Yuting
    Food and Fermentation Industries. 2022, 48(6): 281-289. https://doi.org/10.13995/j.cnki.11-1802/ts.028172
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    Fish is an important part of the daily dietary which with high nutritional value. But during the storage process, due to the fragility of fish tissues, the negative effect of endogenous enzymes and psychrophilic bacteria, the smell, flavor and texture of fish change which result in the quality of fish deteriorates rapidly. Therefore, rapid, convenient and non-destructive detection of fish freshness has become a research hotspot. Based on summarized and compared the traditional detection methods of fish freshness, the new detection methods were summarized, including odor fingerprint technology, intelligent packaging for fish freshness detection, proteomics, biosensor technology and spectrum technology, and their advantages and disadvantages were compared. Finally, their development and application trends also have been prospected. It lays a foundation for the development of new detection technology and promotes its standardization.
  • MA Yaqin, ZHANG Chen, DENG Tujing, ZHOU Jia
    Food and Fermentation Industries. 2022, 48(6): 290-298. https://doi.org/10.13995/j.cnki.11-1802/ts.027781
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    Aroma, as a key index for consumers to measure the quality of fruits and their processed products, can significantly affect consumers' acceptance of fruits and their processed products. The aroma mainly exists in the form of free state and bonded state, and glycoside bonded aroma substances are not easy to be perceived directly. The study of glycoside bonded aroma substances is very important for improving, balancing and restoring the flavor of fruits and their processed products. This article reviewed the structure, extraction and analysis methods, aroma release methods, and the odor and retention index of some common aroma compounds in fruits and their processed products, in order to provide a theoretical reference for the industrial application of fruit aroma in the future.
  • YANG Xudong, GUO Chuo, YUAN Kai, YANG Xi, WANG Xiaoyu, MENG Yonghong, GUO Yurong
    Food and Fermentation Industries. 2022, 48(6): 299-308. https://doi.org/10.13995/j.cnki.11-1802/ts.028025
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    With the rapid development of the modern food industry and the gradually increasing health awareness of the public, developing the novel food products with good health, nutrition and sensory attributes has become an urgent task in the food industry. Low methoxyl pectin can form gels only in the presence of calcium ions, with sucrose not required. This property is highly appreciated in producing low calorie-based food products. This paper firstly reviewed the structure characteristics and gelation mechanism of low methoxyl pectin, highlighting the effects of various factors on low methoxyl pectin gels. Furthermore, the application of low methoxyl pectin in developing novel food products was also summarized, which providing the foundation for further applications of low methoxyl pectin in the food industry.
  • LI Mengyu, YANG Ziyi, CHEN Jia, ZHAO Guohua
    Food and Fermentation Industries. 2022, 48(6): 309-315. https://doi.org/10.13995/j.cnki.11-1802/ts.027976
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    As an indispensable part of the human daily diet, edible vegetable oil can provide energy and other nutrients to the human body. The moisture content in edible vegetable oil is low, which will accelerate the rancidity and microbial growth of edible vegetable oil, and seriously affects the nutritional quality and economic value of edible vegetable oil. Therefore, moisture detection plays a pivotal role in the quality control and quality assurance of edible vegetable oils. Based on the introduction of the classic Karl Fischer method, electrochemistry and chromatography and other edible vegetable oil moisture detection methods, this article focuses on new edible vegetable oil moisture detection methods such as near-infrared spectroscopy and fluorescence probe method. These methods have the advantages of high detection accuracy, fast speed, simple equipment and easy portability, and have good application prospects in real-time monitoring the quality of edible vegetable oil products.
  • PANG Zixuan, WU Jiheng, YAN Hao, WANG Zhiyuan, LI Ye, BAI Zhonghu
    Food and Fermentation Industries. 2022, 48(6): 316-326. https://doi.org/10.13995/j.cnki.11-1802/ts.028069
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    Polyketides are a class of secondary metabolites produced by plants or microorganisms and have a wide range of sources in nature. At present, tens of thousands of polyketides have been discovered. Polyketides have been widely used in agriculture, food industry, medical care and other fields based on their complex chemical structures and abundant physiological activities, including antibacterial activity, antitumor activity, antioxidant activity and so on. In recent decades, many studies have been carried out to deeply understanding the mechanism of polyketide biosynthesis. However, natural polyketides cannot fully meet the needs of society, so the construction of unnatural polyketides has become a research hotspot. Therefore, this review mainly summarized the research progress on the classification, structure and catalytic mechanism of polyketide synthase; some natural polyketide compounds and their hosts; the construction technology and application examples of unnatural polyketide compounds, and the biological activities of polyketide compounds. Finally, the shortcomings of current research on polyketide compounds were pointed out, and the development, as well as the application direction of polyketide compounds in the future were prospected.
  • CHEN Maoshen, XIA Yixun, XU Feifei, LIU Fei, ZHONG Fang
    Food and Fermentation Industries. 2022, 48(6): 327-332. https://doi.org/10.13995/j.cnki.11-1802/ts.030044
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    Under the background of “double first-class” subject construction and “ideological and political curriculum”, the course of food sensory evaluation opened nearly 40 years has been reformed and new course of Food Perception has been opened in the major of Food Science and Technology in Jiangnan university. The teaching framework included food stimuli and their material base of perception, emotion recognition triggered by food stimuli, and future of food design driven by consumer cognition. The ideological and political elements were integrated into the teaching in order to achieve the purpose of improving teaching effect and moral education. In view of the main deficiencies existing in the past teaching process, the preliminary exploration and practice were carried out through the reform and innovation of teaching methods and assessment methods. At present, better teaching effects have been achieved.